• KSBB Journal
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• 제17권2호
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• pp.121-136
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• 2002

Immunosensors are of great interest because of their potential utility as specific, simple, label-free, direct detection means and provision of reduction in size, cost and time of analysis comparing with conventional immunoassay. In the last two decades, many reports have been published on the use of immunosensors for a wide range of applications to clinical diagnostics, pharmaceutical chemistry, environmental monitoring, biotechnology and food industries. There are also numerous transduction techniques developed such as electrochemical techniques, piezoelectric crystal, and surface plasmon resonance receiving much attention for the direct monitoring of immune reactions at solid surfaces. In this article, the principles, characteristics, structures, fonctions and clinical applications of immunosensors were reviewed

• 대한의용생체공학회:의공학회지
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• 제26권5호
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• pp.271-282
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• 2005

There are strong demands for accurate, fast, and inexpensive devices in the medical diagnostic laboratories, such as biosensors and chemical sensors. Biosensors can provide the reliable and accurate informations on the desired biochemical parameters, which is an essential prerequisite for a patient before going for a treatment. They can be used for continuous measurements of metabolites, blood cations, gases, etc. Of these, electrochemical biosensors play an important role in the improvement of public health, because rapid detection, high sensitivity, small size, and specificity are achievable for clinical diagnostics. In this paper, the clinical applications with electrochemical biosensors are reviewed. An attempt is also made to highlight some of the trends that govern the research and developments of the important biosensors that are associated to clinical diagnosis.

• 한국식품위생안전성학회지
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• 제32권2호
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• pp.83-88
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• 2017

본 연구의 목적은 screen printed 탄소계 전극에 기반하여 기능성 식품중에 포함된 소량의 Sildenafil과 Vardenafil(SDF 및 VDF)을 쉽게 검출하기 위한 전류계 면역센서를 개발하고자 하였다. 본 연구에서 개발한 면역센서는 horseradish peroxidase로 labeling 시킨 후 비경쟁적 샌드위치 ELISA 측정법에 의한 원리를 이용하였다. 개발된 센서는 그래핀 산화물 및 키토산(ErGO-CS) 복합체를 단순한 전기화학적 증착에 의해 screen printed 탄소계 전극을 이용하는 원리이며, 감지된 화학물질을 평가하고 센서특성을 최적화하기 위해 전기 화학적 임피던스분광법, 순환 전압 전류법 등을 포함한 일련의 전기화학적 실험방법에 기초하여 완성되었다. 본 연구에서 개발된 센서는 100 pg/mL ~ 300 ng/mL 농도의 SDF 및 VDF에 대하여 직선상의 농도-의존적인 반응을 나타내었다. 또한 최저 검출 한계는 55 pg/mL이었으며, 센서의 민감도는 $1.02{\mu}Ang/mL/cm^2$로 산출되었다. 센서의 성능은 7.1%의 상대 표준편차로 매우 우수한 재현성을 나타내었다. 본 연구에서 개발된 센서의 전략적 가치는 향후 건강기능식품중에 함유된 SDF 및 VDF과 같은 의약품의 미량을 현장에서 쉽게 분석 할 수 있어서 비용-효과적 측면에서 그 가치가 우수하다는 것을 제시한다.

• Bulletin of the Korean Chemical Society
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• 제33권3호
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• pp.839-842
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• 2012

We report for the first time the synthesis of niobium dioxide nanowires on a sapphire substrate by chemical vapor transport method. We identified single crystalline nature of as-synthesized nanowires by scanning electron microscopy and transmission electron microscopy. Niobium dioxide nanowires with their large surface-to-volume ratio and high activities can be employed for electrochemical catalysts and immunosensors. The Raman spectrum of niobium dioxide nanowires also confirmed their identity.

• 센서학회지
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• 제20권1호
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• pp.35-39
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• 2011

A disposable electrochemical immunosensor system has been developed for the detection of herbicide in aqueous samples. Disposable screen printed carbon electrodes(SPCE) were used as basic electrodes and an enzyme, horseradish peroxidase (HRP), and anti-herbicide antibodies was immobilised on to the working electrode of SPCE by using avidin-biotin coupling reactions. An herbicide-glucose oxidase conjugates have been used for the competitive immunoreaction with sample herbicides. The enzymatic reaction between the conjugated glucose oxidase and glucose added generates hydrogen peroxide, which was reduced by the peroxidase immobilised. The latter process caused an electrical current change, due to direct re-reduction of peroxidase by a direct electron transfer mechanism, which was measured to determine the herbicides in the sample. The optimal operational condition was found to be: $20\;{\mu}gl-1$ deglycosylated avidin loading to the working electrode and working potential +50 mV vs. Ag/AgCl. The total assay time was 15 min after sample addition. The detection limits for herbicides, atrazine and simazine, were found to be 3 ppb and 10 ppb, respectively.

• 전기학회논문지
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• 제56권8호
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• pp.1424-1429
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• 2007

This paper presents a simple and reliable one-touch type multi-immunosensing lab-on-a-chip (LOC) detecting antibodies as multi-disease markers using electrochemical method suitable for a portable point-of-care system (POCS). The multi-stacked LOC consists of a PDMS space layer for liquids loading, a PDMS valve layer with 50 im in height for the membrane, a PDMS channel layer for the fluid paths, and a glass layer for multi electrodes. For the disposable immunoassay which needs sequential flow control of sample and buffer liquids according to the designed strategies, reliable and easy-controlled on-chip operation mechanisms without any electric power are necessary. The driving forces of sequential liquids transfer are the capillary attraction force and the pneumatic pressure generated by air bladder push. These passive fluid transport mechanisms are suitable for single-use LOC module. Prior to the application of detection of the antibody as a disease marker, the model experiments were performed with anti-DNP antibody and anti-biotin antibody as target analytes. The flow test results demonstrate that we can control the fluid flow easily by using the capillary stop valve and the PDMS check valves. By the model tests, we confirmed that the proposed LOC is easily applicable to the bioanalytic immunosensors using bioelectrocatalysis.