• 대한물리치료과학회지
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• 제9권4호
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• pp.53-61
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• 2002

This study was carried out to investigate the effects of electrical stimulation on the change oxytocin(OT) immunoreactive cells in the hypothalamus of the male SD rats. Fifteen healthy and normal male rats were divided into three groups, 5 SD rat in each group. The one group has been stimulated by electro-acupuncture(EA, 2Hz) for 30 min and the other group by EA for 1hr 30 min and control group has not been stimulated. The results were summarized as follows. OT immunoreactive cells were found in the Paraventricular nucleus (PVN), Supraoptic nucleus(SON) and Lateral Magnocellular Area(LMA). The number of OT immunoreactive cells of PVN were significantly increased after 30 min as compared with control group (p<0.05) and were also significantly increased after 1hr 30min than 30 min(p<0.05). The number of OT immunoreactive cells of SON were significantly increased after 30 min and 1hr 30 min(p<0.05) as compared with control group, but decreased after 1hr 30 min than 30 min. The number of immunoreactive hells of LMA significantly increased after 30 min(p<0.05) as compared with control group, but significantly decreased after 1hr 30 min than 30 min(p<0.05). These results show that OT is released into the hypothalamus in response to electrical stimulation.

• The Journal of Korean Physical Therapy
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• 제11권3호
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• pp.45-55
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• 1999

This study was compared with each 2 groups (Intermittent traction therapy alone group1: Control group and Intermittent traction therapy plus Auriculotherapy group 2: Experimental group) on 20 HIVLD(Hemiated Intervertebral Lumbar Disk) patients with sciatics Each subjects were divided to control and experimental. Each group were applied Intermittent traction therapy (HOld:25, Rest:15) for 15 minute and control group were not received auricular stimulation. However experimental groups were applied auricular stimulation simultaneosly. Auricular acupuncture points(max-17, min=13) were stimulated with low frequency. high intensity Electro-Acupunture stimulator for 20 seconds per each point. Treatment and measurements(R.P and VAS) was administered to each patient during the 10 times therapy per 2 weeks. The results were significant differences between the two groups. Experimental group appeared significantly greater than before in pain, and pain relief, and significant improvement in centralization phenomenon of radiating pain.

• 한방안이비인후피부과학회지
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• 제14권2호
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• pp.224-230
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• 2001

In the Clinical study on 1 Case of Patient with Obstruction of the retinal artery, the results were as follows. 1. Obstruction of the retinal artery is acute disease that vision isdiminished fastly and become a blindness. 2. It belong to the cathegory of Pock Maeng(暴盲) in Oriental Medicine. 3. The causes of Obstruction of the retinal artery were the yin-deficiency of liver and kidney, the stagnation of vital energy and blood stasis, the abundance of phlegm-heat, the yang-deficiency of spleen and kidney, the yin-deficiency of spleen and kidney, the nonfastness of primordial energy etc. 4. We could get the effective result by providing the patient with Jinkansikpung-tang-gami(鎭肝熄風湯加味) in the acute period 5. In Acupuncture treament and other treament, Electro-acupuncture theraphy ana Lodestone theraphy were useo for neurologic stimulation, Body acupuncture and Auricula-acupuncture were used for promoting the circulation of vital energy and blood.

• 동의신경정신과학회지
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• 제21권1호
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• pp.59-69
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• 2010

Objectives: The study aims to look into how the application of EA(Electro-acupuncture) in the initial step of ischemic brain injury affects the changes in Bax and Caspase-3 expression among forebrain apoptosis. Methods: It caused GI by using common carotid artery occlusion and conducted the test by dividing into the no applied EA group after 6 hours and 12 hours, the LI 4 EA group that applied EA to LI4, and GV 20 EA group that was applied to GV 20. After that, the following results were obtained by comparing Bax and Caspase-3 expression, which were apoptosis-related factors, with its application time through immunohistochemical staining after extracting brain of each group. Results: According to each group's Bax expression by the application time, the order of expression increase after 6 hours is shown to be the no applied EA group, LI 4 group, and GV 20 group. The stimulation after 12 hours was most lowly expressed in the GV 20 group, and the no applied EA group and LI 4 group showed similar level. Conclusions: EA stimulation in the initial step after ischemia seems to affect positively Bax and Caspase-3 expression that are index of forebrain apoptosis.

• Korean Journal of Acupuncture
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• 제29권1호
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• pp.83-92
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• 2012

Objectives : The purpose of this research was to develop the magnetic acupuncture system which used solenoid coil for magnetizing acupuncture needle. The system could generate the meridian electric potential (MEP) similar to the potential by manual acupuncture. Thus, we tried to confirm the therapeutic effect that is caused by the MEP generation. Methods : To confirm the MEP, we stimulated the magnetic acupuncture with at 2Hz, $92.7{\pm}2mT$, PEMFs (Pulsed Electro-Magnetic Fields) at ST37 and measured the evoked potential between ST36 and ST41. Also, we conducted a fatigue recovery test using isokinetic exercise in order to identify the therapeutic effect on musculoskeletal disorders. We chose LR9 as a stimulation point. To observe the state of fatigue, we measured the EMG and analyzed median frequency and peak torque for 20minutes. Results : We observed that MEP which incurred from magnetic acupuncture was higher than he reported MEP induced by manual acupuncture. Moreover, its modes were divided into two types by the direction of magnetic flux. When generating magnetic flux in the direction of acupoint, the positive peak voltage of the MEP was generated. In contrast, negative peak voltage of the MEP was generated whenever meganetic flux generated in the outward direction. As a result of fatigue recovery, the median frequency (MF) of the magnetic acupuncture group were recovered faster than that of the non-stimulation group. However, the peak torques of both groups were not restored until after 20 minutes. Conclusions : We confirmed that the magnetic acupuncture system can lead to the MEP similar to manual acupuncture. Moreover, the MEP had a therapeutic effect on the musculoskeletal disorders.

• 대한물리치료과학회지
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• 제7권2호
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• pp.513-521
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• 2000

This study was carried out to investigate the effects of electrical stimulation on the change Tyrosine-Hydroxylase immunoreactive(TH-IR) cells in the Central gray and retrorubral field of the male SD rats. 9 healthy and normal rats were divided into three groups, 3 SD rat in each group. The one group has been stimulated by electroacupuncture(EA, 2Hz) for 30min and the other group by EA for 1hr 30min and control group has been stimulated. TH-IR cells were found in the Central gray(CG) and Retrorubral field(RF). The numbers of TH-IR cells of CG and RF were significantly increased after 30 min (CG $6.2{\pm}0.83$, RF $1.4{\pm}0.55$)as compared with control group(CG $24{\pm}3.16$, RF $6.4{\pm}0.55$) and were also significantly increased after 1hr 30min(CG $75.6{\pm}4.51$, RF $18.8{\pm}0.89$) than 30min. These results show that TH is related into CG and RF in response to electrical stimulation.

• 대한한의학회지
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• 제30권3호
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• pp.70-78
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• 2009

Objectives : This study was aimed at examining the effects of the application of EA (electroacupuncture) at GV20 and LI4 in the early cerebral ischemia on the size of cerebral infarction, COX-2 and IL-6. Methods : For this experiment, 21, six-week-old male S-D (Sprague - Dawley) rats weighting 160g to 200g were selected and randomly classified into 3 groups, seven rats in each group. Brain ischemia was simulated using a modified Koizumi method which was performed on each rat. In the GV20 group, the GV20 of the SD rats was stimulated for thirty minutes with acupunctural electrode low frequency stimulator five hours after inducement of ischemia. For the LI4 group, the LI4 was stimulated as above, while for the Ischemia group, no stimulation was applied. Twenty-four hours after the experiment, stained cerebral tissues were examined and an immuno-histological test was done to examine inflammatory reaction Results : Out of the three groups, the LI4 group showed the smallest size of cerebral infarction and the Ischemia group showed the highest COX-2 (cyclooxygenase-2) expression value in the cortex of the cerebrum. In addition, the LI4 group showed the lowest COX-2 expression value in unknown putamen out of the three groups. Conclusions : We infer that EA, applied at LI4 and GV20 in early ischemia, is effective in delaying the expression of IL-6 (interleukin-6) and COX-2, the inflammatory agents manifested from stroke. In addition, application at LI4, rather than GV20, can lower the expression value of the inflammatory agents. Further, EA can be an effective way to block early inflammatory reaction in stroke.

• 대한물리치료과학회지
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• 제6권2호
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• pp.977-988
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• 1999

This study compared the effects of Intermittent traction therapy alone(Group1=Control group) and Intermittent traction therapy plus Auriculotherapy (Group2=Experimental group) to 20 Sciatica patients. Subjects were assigned to two groups with each 10. The two groups received Intermittent traction therapy (Hold:25, Rest:15) for 15 minute and experimental group did received auricular stimulation. Auricular acupuncture points(max=17, min=13) were stimulated with low frequency, high intensity Electro-Acupunture stimulator for 20 seconds per a each point. Treatment and measurements(SLR and VAS) was administered to each patient during the two weeks of 10 times therapy. The results were significant differences between the two groups. Experimental group produced significantly greater pain relief, and significant improvement of limited SLR.

• Journal of Acupuncture Research
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• 제34권1호
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• pp.23-30
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• 2017

Objectives : In this study, the roles of Interleukin (IL)-4 and Signal transducer and activator of transcription 6 (STAT6), which have been reported to play a role in the pathogenesis of inflammation and cancer, were evaluated in snake venom toxin (SVT)-induced apoptosis. Methods : Inflammation was induced in human HaCaT kerationocytes, by lipopolysaccharide (LPS; $1{\mu}g/mL$) or tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), followed by treatment with SVT (0, 1, or $2{\mu}g/mL$). Cell viability was assessed by MTT assays after 24 h, and the expression of levels of IL-4, STAT6, and the apoptosis-related proteins p53, Bax, and Bcl-2 were evaluated by western blotting. Electro mobility shift assays (EMSAs) were performed to evaluate the DNA binding capacity of STAT6. Results : MTT assays showed that inflammation-induced growth of HaCaT cells following LPS or TNF-${\alpha}$ stimulation was inhibited by SVT. Western blot analysis showed that p53 and Bax, which promote apoptosis, were increased, whereas that of Bcl-2, an anti-apoptotic protein, was decreased in a concentration-dependent manner in LPS- or TNF-${\alpha}$-induced HaCaT cells following treatment with SVT. Moreover, following treatment of HaCaT cells with LPS, IL-4 concentrations were increased, and treatment with SVT further increased IL-4 expression in a concentration-dependent manner. Western blotting and EMSAs showed that the phosphorylated form of STAT6 was increased in HaCaT cells in the context of LPS- or TNF-${\alpha}$-induced inflammation in a concentration-dependent manner, concomitant with an increase in the DNA binding activity of STAT6. Conclusion : SVT can effectively promote apoptosis in HaCaT cells in the presence of inflammation through a pathway involving IL-4 and STAT6.

• Korean Journal of Acupuncture
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• 제21권2호
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• pp.1-28
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• 2004

Objectives : Human physiological changes in the state of qigong has been measured using EEG(Electroencephalography), functional MRI(functional Magnetic Resonance Image), EAV(Electro-Acupuncture according to Voll) and SQUID(Superconducting Quantum Interference Device) measurements. Methods & Results : EEGs were measured to study the differences between Qigong masters and Qi receiver on the changes of EEG. During Qigong, an alpha waves were increased. The power spectra indicate that the peak frequency of alpha waves increased during Qigong. Qi receiver's EEG signals seemed to affected by the state of himself. Brain activation did not observed when qigong master concentrates the Qi at Laogong(P8). But a localization of fMRI signal in the sensory cortex was observed by electric acupuncture stimulation at Laogong(P8). Five phase deviation of EAV were clearly changed in the both cases of Qigong master and Qi receiver. When a Qigong master concentrates the Qi at Yintang, Laogong(P8), Qihai(CV6) meridian points during Qigong state, the change of magnetic field around acupoints Yintang, Laogong points has been measured using 40-Channel DROS-SQUID apparatus. After smoothing process of the continuously measured magnetic signal around acupoints for a few minutes, we could observe that a series of peaks, magnitude of -1.0~2.5pT appeared. But there was no significant difference in changes of magnetic signal around acupoints. Physical signals of magnetocardiogram has been measured by using 2-Channel DROS SQUID(Magnetocardiogram). Physical signals of magnetocardiogram were clealy changed at the ST segments after S-wave when qigong master concentrates the Qi.