• International Journal of Oral Biology
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• 제44권1호
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• pp.14-19
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• 2019

The present study aimed at evaluating serum immunoglobulin G (IgG) avidity to Porphyromonas gingivalis in elderly patients with mild and severe chronic periodontitis. The avidity of antibodies against P. gingivalis present in the sera of 18 patients with mild chronic periodontitis and 18 patients with severe chronic periodontitis was evaluated using an ammonium thiocyanate-dissociated enzyme-linked immunosorbent assay (ELISA). The results showed that the mean absorbance value in serum IgG antibody titers was significantly higher in the severe chronic periodontitis group than in the mild chronic periodontitis group ($198{\pm}35ELISA$ unit [EU] vs. $142{\pm}32EU$, p < 0.01). However, there was no significant difference between the two groups in antibody avidity ($65{\pm}57EU$ vs. $54{\pm}27EU$). These findings suggest that humoral immune responses to P. gingivalis between mild and severe chronic periodontitis in elderly patients are characterized by the differences in the quantity rather than the quality of the antibodies.

• 한국의류학회지
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• 제34권5호
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• pp.844-855
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• 2010

An important issue for the improvement of product quality is to identify and understand the customer-required attributes of a product. This paper identifies and analyzes customer-required attributes of formal knitwear for middle age and elderly women through the use of the Kano model in order to develop competitive products. Various research methods were used in this study. Customer voices for formal knitwear were collected by means of brainstorming and in-depth interviews. The collected customer voices were transformed into customer-required attributes with the use of an affinity diagram. In addition, the data was collected by means of a questionnaire and was statistically analyzed to examine the dimensions of customer-required attributes on the basis of the Kano model. The results are as follows. Five categories of customer-required attributes were identified (symbolism, aesthetic, fitness, usefulness, and maintenance). The analysis based on the Kano model identifies a strong one-dimensional quality in all of the categories of customer-required attributes and the attractive quality that includes a one-dimensional quality in the categories of fitness and usefulness. The findings of this study demonstrate the applicability of the affinity diagram for apparel that educates formal knitwear developers of the customer-required attributes that they should strategically focus on in order to develop competitive products.

• 디지털융복합연구
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• 제15권2호
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• pp.519-525
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• 2017

본 연구는 고령친화 요양산업을 대상으로 정책의 활성화시킬 수 있는 요인과 전략방안들을 도출하는 것이 연구의 목적이다. 분석방법은 DEMATEL기법을 통해 주요 요인들 간의 인과성을 분석하였다. 이는 고령친화 요양산업을 구성하는 활성화 요인 및 그 요인 간의 관련이 복잡 불명확한 것을 정책강화의 우선순위 도출을 통해 보다 명확하게 확인하고자 하였다. 분석결과 종합강도가 가장 높게 나타난 활성화 요인으로는 식사서비스(22.095)가 가장 종합강도가 높은 것으로 분석되었다. 그리고 다음으로 건강증진시설(19.97), 건강관리(17.726) 등의 순으로 높게 나타났다. 가장 낮은 요인은 스포츠(15.896)이다. 따라서, 이러한 요인의 개선 및 증진을 위해서 시설요양서비스, 재가요양서비스, 예방지원서비스와 밀접한 연관성을 가지고 지속적인 정책방안을 모색하여야 할 것이다.

• 대한화학회지
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• 제58권6호
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• pp.553-559
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• 2014

Alzheimer's disease (AD) is a devastating neurodegenerative disease that represents the most common form of dementia among the elderly population. The deposition of aggregated ${\beta}$-amyloid ($A{\beta}$) senile plaques in the human brain is a classic observation in the neuropathology of AD, yet an understanding of the mechanism of their formation remains elusive. $A{\beta}$ is formed through endoproteolysis of the amyloid precursor protein (APP) by ${\beta}$-secretase (BACE1, ${\beta}$-site APP-cleaving enzyme) and ${\gamma}$-secretase. In this study, BACE1 protein was successfully over-expressed, purified, and refolded and utilized in a binding study with hispidin. We developed a simpler refolding method using a urea gradient and size-exclusion gel filtration to purify an active BACE1 protein variant, in larger quantities than that reported previously, and measured the binding affinity of hispidin to the BACE1 protein variant through isothermal titration calorimetry.

• International Journal of Oral Biology
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• 제39권4호
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• pp.221-228
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• 2014

Glucosamine is commonly taken by the elderly without prescription as a nutritional supplement to attenuate the progression or symptoms of osteoarthritis. Previous studies demonstrated that glucosamine shows anti-inflammatory effects in tissues such as blood vessels and the heart. However, there have been few reports about the effects of glucosamine on oral inflammatory diseases. Therefore, in this study, the effects of glucosamine on lipopolysaccharide (LPS)-induced inflammatory responses were investigated using human periodontal ligament fibroblasts (HPDLFs). HPDLFs were incubated in the presence and absence of glucosamine (10 mM) for 24 h, followed by treatment with E. coli LPS (100 ng/ml) or vehicle. Quantitative RT-PCR and ELISA results showed that LPS exposure significantly increased the levels of IL-6 and IL-8 mRNA and protein, while the effect was significantly suppressed by glucosamine treatment. Glucosamine did not attenuate, but slightly increased, the LPS-induced activation of mitogen activated kinases (ERK, p38, JNK). However, it suppressed the LPS-induced increase in the DNA binding affinity and transcriptional activity of NF-${\kappa}B$. These results suggest that glucosamine exerts anti-inflammatory effects on HPDLFs exposed to LPS via inhibition of NF-${\kappa}B$ activity, necessitating further studies using animal periodontitis models.

• Toxicological Research
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• 제17권
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• pp.47-57
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• 2001

Alzheimer's disease (AD) is the most common cause of dementia in the elderly, and its pathology is characterized by the presence of numerous numbers of senile plaques and neurofibrillary tangles. Several genetic and transgenic studies have indicated that excess amount of $\beta$-amyloid protein (A$\beta$) is produced by mutations of $\beta$TEX>$\beta$-amyloid precursor protein and causes learning impairment. Moreover, $A\beta$ has a toxic effect on cultured nerve cells. To prepare AD model animals, we have examined continuous (2 weeks) infusion of $A\beta$ into the cerebral ventricle of rats. Continuous infusion of $A\beta$ induces learning impairment in water maze and passive avoidance tasks, and decreases choline acetyltransferase activity in the frontal cortex and hippocampus. Immunohistochemical analysis revealed diffuse depositions of $A\beta$ in the cerebral cortex and hippocampus around the ventricle. Furthermore, the nicotine-evoked release of acetylcholine and dopamine in the frontal cortex/hippocampus and striatum, respectively, is decreased in the $A\beta$-infused group. Perfusion of nicotine (50 $\mu\textrm{M}$) reduced the amplitude of electrically evoked population spikes in the CA1 pyramidal cells of the control group, but not in those of the $A\beta$-infused group, suggesting the impairment of nicotinic signaling in the $A\beta$-infused group. In fact, Kd, but not Bmax, values for ［$^3H$］ cytisine binding in the hippocampus significantly increased in the $A\beta$-infused rats. suggesting the decrease in affinity of nicotinic acetylcholine receptors. Long-term potentiation (LTP) induced by tetanic stimulations in CA1 pyramidal cells, which is thought to be an essential mechanism underlying learning and memory, was readily observed in the control group, whereas it was impaired in the $A\beta$-infused group. Taken together, these results suggest that $A\beta$ infusion impairs the signal transduction mechanisms via nicotinic acetylcholine receptors. This dysfunction may be responsible, at least in part, for the impairment of LTP induction and may lead to learning and memory impairment. We also found the reduction of glutathione- and Mn-superoxide dismutase-like immunoreactivity in the brains of $A\beta$-infused rats. Administration of antioxidants or nootropics alleviated learning and memory impairment induced by $A\beta$ infusion. We believe that investigation of currently available transgenic and non-transgenic animal models for AD will help to clarify the pathogenic mechanisms and allow assessment of new therapeutic strategies.

• 한국콘텐츠학회논문지
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• 제16권12호
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• pp.667-676
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• 2016

본 연구는 한국의 사회적기업이 사회적 경제의 핵심조직으로 급격히 성장하고 있는 상황에서 사회적기업과 영리기업종사자들을 대상으로 조직문화의 차이를 확인하고, 조직성과에 미치는 영향을 평가하였다. 주요 결과는 다음과 같다. 첫째, 사회적기업과 영리기업종사자의 특성차이 분석결과 사회적기업이 여성비율이 많고, 연령이 높았으며 연봉은 영리기업이 많았다. 사회적기업이 저임금의 여성 노인을 많이 채용하고 있음을 알 수 있다. 둘째, 조직문화차이 분석결과 합리문화는 영리기업이 높고, 집단문화는 사회적기업이 높았다. 영리기업이 생산성과 효율성, 계획수립과 목표설정, 목표달성 위주의 평가, 실적위주의 평가를 중요시하는 반면 사회적기업이 친화와 참여, 종업원 개개인의 개발, 집단사기와 응집력, 상호협조 및 신뢰감을 중요시하고 있었다. 또한, 사회적성과와 경제적성과 모두 사회적기업이 영리기업에 비하여 높았다. 사회적기업이 종사자 참여와 이해에 기반하여 투명하게 조직을 운영하고 있어서 경제적성과도 높게 인식하는 것으로 판단된다. 셋째, 사회적성과는 영리기업은 개발문화 집단문화, 사회적기업은 개발문화 집단문화 위계문화가 높을수록 증가하였다. 경제적성과는 영리기업은 합리문화가 낮을수록 증가하였고, 사회적기업은 집단문화가 높을수록 증가하였다. 아울러 사회적기업 종사자들이 영리기업종사자들에 비하여 사회적성과와 경제적 성과가 높다고 인식하고 있었다. 요컨대, 한국 사회적기업이 영리기업과 차별적인 조직문화를 형성해나가고 있으며, 이러한 조직문화가 조직성과를 달성하는데 기여하고 있는 것으로 판단된다.