• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.624-631
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• 2007

Thermostable protease is very effective to improve the industrial processes in many fields. Two thermostable extracellular proteases from the culture supernatant of the thermophilic fungus Chaetomium thermophilum were purified to homogeneity by tractional ammonium sulfate precipitation, ion-exchange chromatography on DEAE-Sepharose, and Phenyl-Sepharose hydrophobic interaction chromatography. By SDS-PAGE, the molecular mass of the two purified enzymes was estimated to be 33 kDa and 63 kDa, respectively. The two proteases were found to be inhibited by PMSF, but not by iodoacetamide and EDTA. The 33 kDa protease (PRO33) exhibited maximal activity at pH 10.0 and the 63kDa protease (PRO63) at pH5.0. The optimum temperature for the two proteases was $65^{\circ}C$. The PRO33 had a $K_m$ value of 6.6mM and a $V_{max}$ value of $10.31{\mu}mol/l/min$, and PRO63 l7.6mM and $9.08{\mu}mol/l/min$, with casein as substrate. They were thermostable at $60^{\circ}C$. The protease activity of PRO33 and PRO63 remained at 67.2% and 17.31%, respectively, after incubation at $70^{\circ}C$ for 1h. The thermal stability of the two enzymes was significantly enhanced by $Ca^{2+}$. The residual activity of PRO33 and PRO63 at $70^{\circ}C$ after 60min was approximately 88.59% and 39.2%, respectively, when kept in the buffer containing $Ca^{2+}$. These properties make them applicable for many biotechnological purposes.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.189-196
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• 1992

A $\alpha$-l, 4-D-glucan maltohydrolase $(\beta$-amylase), secreted by the mesophilic aerobic bacterium Bacillus polymyxa No.26, was purified and characterized. The enzyme production was increased after a logarithmic phase of bacterial growth and paralleled with the onset of bacterial sporulation. By applying anion exchange chromatography and gel filtration the enzyme was purified 16.7-fold and had a specific activity of 285.7 units/mg. Two enzyme activities were eluted on a column of DEAE-Sephadex chromatography, and they were designated as E-I for a major enzyme peak and E-II for a minor peak. Of them, E-I enzyme peak was further purified by using gel chromatography. The molecular mass of this enzyme was determined to be 64, 000 daltons and consisted of a single subunit, showing an isoelectric point of 8.9. The enzyme was able to attack specifically the $\alpha$-l, 4-glycosidic linkages in soluble starch and caused its complete hydrolysis to maltose and $\beta$-limited dextrin. This amylolytic enzyme displayed a temperature optimum at $45^\circ{C}$ and a pH optimum at 7.0. The amino acid composition of the purified enzyme was quite similar to the other bacterial $\beta$-amylases reported. Surprisingly, the purified enzyme from this aerobe only exhibited hydrolytic activity on soluble starch, not on starch granules. The degradation of from starch by $\beta$-amylase was greatly stimulated by pullulanase addition. These results differentiated from other $\beta$-amylases reported. Based on a previous result that showed the enzyme system involves in effective degradation of raw starch granules, this result strongly suggested that the purified enzyme (E-I) can be a synergistic part of starch granule-digestion and E-II plays a crucial role in digestion of starch granules.

• Transactions of the Korean Society of Automotive Engineers
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• v.21 no.1
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• pp.99-106
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• 2013

DME (Di-Methyl Ether) is synthetic product that is produced through dehydration of methanol or a direct synthesis from syngas. And it is able to save fossil fuel and reduce pollutants of emission such as PM and $CO_2$. In spite of its advantages it is difficult to design DME fuelled engine system because DME fuel may cause to severely generate cavitation and corrosion in fuel delivery system due to physical properties of DME. Therefore, in this study three-dimensional internal flow characteristics with consideration of cavitation were predicted in the DME injector using diesel and DME fuel. Moving grid technique was employed to describe needle motion and 1-D hydraulic simulation of injector was also simulated to obtain transient needle motion profiles. The results of simulation show that cavitations was generated at the inlet of nozzle near high velocity region both diesel and DME. And mass flow rate of DME is reduced by 4.73% compared to that of diesel at maximum valve lift because cavitation region of DME is much more larger. To increase flow rate of DME injector, internal flow simulation has been conducted to investigate the nozzle hole inner R-cut effect. The flow rates of diesel and DME increase as R-cut increases, and flow coefficient of DME fuel injector was increased by 6.3% on average compared with diesel fuelled injector. Finally, optimum shape of DME injector nozzle is suggested through the comparison of flow coefficient with variation of nozzle hole inner R-cut.

• Progress in Superconductivity and Cryogenics
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• v.16 no.3
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• pp.21-25
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• 2014

As wireless power transfer (WPT) technology using strongly coupled electromagnetic resonators is a recently explored technique to realize the large power delivery and storage without any cable or wire, this technique is required for diffusion of electric vehicles (EVs) since it makes possible a convenient charging system. Typically, since the normal conducting coils are used as a transmitting coil in the CPT system, there is limited to deliver the large power promptly in the contactless EV charging system. From this reason, we proposed the combination CPT technology with HTS transmitting antenna, it is called as, superconducting contactless power transfer for EV (SUWPT4EV) system. As the HTS coil has an enough current density, it can deliver a mass amount of electric energy in spite of a small scale antenna. The SUCPT4EV system has been expected as a noble option to improve the transfer efficiency of large electric power. Such a system consists of two resonator coils; HTS transmitting antenna (Tx) coil and normal conducting receiver (Rx) coil. Especially, the impedance matching for each resonator is a sensitive and plays an important role to improve transfer efficiency as well as delivery distance. In this study, we examined the improvement of transmission efficiency and properties for HTS and copper antennas, respectively, within 45 cm distance. Thus, we obtained improved transfer efficiency with HTS antenna over 15% compared with copper antenna. In addition, we achieved effective impedance matching conditions between HTS antenna and copper receiver at radio frequency (RF) power of 370 kHz.

• Fire Science and Engineering
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• v.28 no.2
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• pp.34-39
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• 2014

It is necessary to enhance its availability and reliability of performance based fire design of building with various type of database such as experiments, survey and fire properties and so on. In order to utilize to the performance based fire design, the present study has been performed a series of experiments to investigate the burning characteristics of building materials for two types of interior board and three types of interior louver. The burning test has been also conducted for different thickness because it may show different characteristics of burning behavior such as flame spread rate and flame propagation time. The result shows that the effective heat release per unit mass of interior materials were almost constant with 15.3~16.9 MJ/kg regardless of its thickness while the peak heat release rate and maximum $CO_2$ concentration was varied with thickness.

• Journal of Korean Association for Spatial Structures
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• v.12 no.2
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• pp.65-72
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• 2012

In this stury, control performance of tuned mass damper (TMD) with torsional rigidity for an eccentric structure showing torsional responses is investigated. To this end, an eccentric structure subjected to earthquake excitation is used to evaluate the control performance of torsional TMD by varying installed location and torsional rigidity of TMD, To reduce computational time required for repetitive time history analysis of an example structure having non-proportional damping system due to TMD, an equivalent analytical model is used in this study. Torsional properties of TMD usually neglected in typical TMD are verified to be effective in reduction of torsional responses of the eccentric structure. In the case of eccentric structures, it has been seen that the center of a plane of a structure may not be optimal location of TMD.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.100-106
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• 2020

Coix lacryma-jobi (Hanjeli) is known to posses anti-microbial properties. Therefore, phytochemical compounds of C. lacryma-jobi have been studied to produce novel antimicrobial agents as treatments against antibiotic-resistant bacteria.The objective of this study was to determine the phytochemical composition and antibacterial activity of the C. lacryma-jobi oil against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The phytochemical composition of the oil was determined via gas chromatography mass spectrophotometry (GC-MS). Moreover, agar disk and agar well diffusion were employed to screen the antibacterial activity of the oil. An agar well diffusion test was implemented to determinate MIC's (minimum inhibitory concentrations). Dodecanoic acid, tetradecanoic acid, 2,3-dihydroxypropylester, 1,3-dioctanoin, N-methoxy-N-methyl-3,4-dihydro-2H-thiopyran6-carboxamide, propanamide, 5-Amino-1-(quinolin-8-yl)-1,2,3-triazole-4-carboxamide, and pyridine were identified in the C. lacryma-jobi oil. The MIC value of the oil was 0.031 g/L and the MBC of the oil was 0.125 g/L effective in all test bacteria. Dodecanoic acid displayed inhibitory activity against gram-positive and gram-negative bacteria. Therefore, our research demonstrated C. lacryma-jobi (Hanjeli) oil exhibited antibacterial activity against E. coli, S. aureus, and B. subtilis. These research suggest that C. lacryma-jobi root oil could be used for medicinal purposes; however clinical and in vivo tests must be performed to evaluate its potential as an antibacterial agent.

• Journal of Microbiology and Biotechnology
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• v.32 no.1
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• pp.99-109
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• 2022

This study is the first report on production and characterization of the enzyme from an Ornithinibacillus species. A 4.2-fold increase in the extracellular protease (called L9^T) production from Ornithinibacillus caprae L9^T was achieved through the one-factor-at-a-time approach and response surface methodological optimization. L9^T protease exhibited a unique protein band with a mass of 25.9 kDa upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This novel protease was active over a range of pH (4-13), temperatures (30-80℃) and salt concentrations (0-220 g/l), with the maximal activity observed at pH 7, 70℃ and 20 g/l NaCl. Proteolytic activity was upgraded in the presence of Ag⁺, Ca²⁺ and Sr²⁺, but was totally suppressed by 5 mM phenylmethylsulfonyl fluoride, which suggests that this enzyme belongs to the serine protease family. L9^T protease was resistant to certain common organic solvents and surfactants; particularly, 5 mM Tween 20 and Tween 80 improved the activity by 63 and 15%, respectively. More importantly, L9^T protease was found to be effective in dehairing of goatskins, cowhides and rabbit-skins without damaging the collagen fibers. These properties confirm the feasibility of L9^T protease in industrial applications, especially in leather processing.

• Journal of the Korean Electrochemical Society
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• v.25 no.1
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• pp.32-41
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• 2022

Various steps in the electrode production process, such as slurry mixing, slurry coating, drying, and calendaring, directly affect the quality and, consequently, mechanical properties and electrochemical performance of electrodes. Herein, a new method of slurry coating is developed: Double-coated electrode. Contrary to single-coated electrode, the cathode is prepared by double coating, wherein each coat is of half the total loading mass of the single-coated electrode. Each coat is dried and calendared. It is found that the double-coated electrode possesses more uniform pore distribution and higher electrode density and allows lesser extent of particle segregation than the single-coated electrode. Consequently, the double-coated electrode exhibits higher adhesion strength (74.7 N m^-1) than the single-coated electrode (57.8 N m^-1). Moreover, the double-coated electrode exhibits lower electric resistance (0.152 Ω cm^-2) than the single-coated electrode (0.177 Ω cm^-2). Compared to the single-coated electrode, the double-coated electrode displays higher electrochemical performance by exhibiting better rate capability, especially at higher C rates, and higher long-term cycling performance. Despite its simplicity, the proposed method allows effective electrode preparation by facilitating high electrochemical performance and is applicable for the large-scale production of high-energy-density electrodes.

• Nuclear Engineering and Technology
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• v.55 no.4
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• pp.1218-1224
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• 2023

We investigated the radiation shielding competence for TeO₂-V₂O₅-MoO₃ glasses. The Phy-X software was used to report the radiation shielding parameters for the present glasses. With an increase in TeO₂ and MoO₃ content, the samples' linear attenuation coefficient improves. However, at low energies, this change is more apparent. At low energy, the present samples have an effective atomic number (Z_eff) that is relatively high (in order of 16.17-24.48 at 0.347 MeV). In addition, the findings demonstrated that the density of the samples is a very critical factor in determining the half value layer (HVL). The minimal HVL for each sample can be found at 0.347 MeV and corresponds to 1.776, 1.519, 1.391, 1.210 and 1.167 cm for Te1 to Te5 respectively. However, the highest HVL of these glasses is recorded at 1.33 MeV, which corresponds to 3.773, 3.365, 3.218, 2.925 and 2.908 cm respectively. The tenth value layer results indicate that the thickness of the specimens needs to be increased in order to shield the photons that have a greater energy. Also, the TVL results demonstrated that the sample with the greatest TeO₂ and MoO₃ concentration has a higher capacity to attenuate photons.