• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.188-195
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• 1993

In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

• Journal of the Korea Concrete Institute
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• v.19 no.1
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• pp.47-56
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• 2007

Recently, tunnels are increasingly constructed in this country with the increased construction of highways, high-speed railways and subways. Shotcrete is one of the major processes in the tunnel construction. Many problems, however, exist in the current shotcrete practice. The purpose of the study is, therefore, to explore the trobles in the current shotcreting practice, and to develop high-quality silica fume shotcrete. For the purpose of security a long-term durability of shotcrete, this study conducted combined deterioration tests. In this study, a combined deterioration test in consideration of a variety of deterioration factors were proceeded. Especially, micro-silica fume that was used frequently in overseas because of a outstanding strength-promotion effect was applied to combined deterioration test, and a long-term durability of shotcrete was investigated according to additions mixing. As a result of test, the shotcrete mixed Micro-silica fume showed a good deterioration quality compared with the other mixes. And is shows that the Micro-silica fume has an outstanding strength-promotion effect and is effective to secure a long-term durability of shotcrete by means of decreasing a deterioration caused by steel fiber mixed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.2
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• pp.475-483
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• 2017

Geosmin and 2-methylisoborneol (2-MIB) produced by cyanobacteria during algal blooming in surface water are the major taste-and-odor-causing compounds in drinking water and need to be removed. Activated carbon is often used in treatment plants for the mitigation of odor problem. However, there is a lack of information on the effect of pore size distribution and particle size of activated carbon for adsorption of both odor compounds. Therefore, we studied the effect of pore size distribution and particle size of activated carbon on the adsorption of geosmin and 2-MIB. When comparing the adsorption of geosmin and 2-MIB between activated carbon fiber (ACF), powdered activated carbon (PAC) and granular activated carbon (GAC), the order of removal efficiency was PAC > ACF > GAC. As a result of comparing PACs with various pore distribution characteristics, well-developed micropores on activated carbon were found to be favorable for adsorption of geosmin and 2-MIB. For particle size, smaller was more effective for adsorption of geosmin and 2-MIB.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.213-217
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• 2000

In order to select more proper plants for phytoextraction at the heavy metal polluted areas, 11 species of non-edible plants were cultivated at the cadmium(Cd) and Lead(Pb) treated soils and analyzed the content of the absorbed Cd and Pb in each part of plants. Plants include three fibers(Linum usitatissimum, Cannabis sativa, Gossypium spp.), three flowers(Calendula officinalis, Rhododendron lateritium, Portulaca grandiflora), and five trees(Pinus thunbergii, Magnolia kobus, $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla). Yield of tree species were higher than that of fiber and flower species. Cd and Pb were highly accumulated in root rather than leaves and stems. The Cd content of plants was in the order Portulaca grandiflora > Calendula officinalis > Gossypium spp. > Linum usitatissimum, Pb was Cannabis sativa > Linum usitatissimum > Fraxinus rhynchophylla. Total absorbed Cd by each plant was in the order $Populus\;nigra\;{\times}\;P.$ maximowiczii > Euonymus japonica > Rhododendron lateritium, but Pb was $Populus\;nigra\;{\times}\;P.$ maximowiczii > Rhododendron lateritium > Euonymus japonica. Total absorbed Cd and Pb contents in plants were negatively correlated with the residual Cd and Pb in the treated soils. It was estimated that $Populus\;nigra\;{\times}\;P.$ maximowiczii, Euonymus japonica, Fraxinus rhynchophylla, and Rhododendron lateritium were the most effective species for phytoextraction in the polluted area considering yield and heavy metal uptake.

• Journal of Life Science
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• v.9 no.2
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• pp.231-240
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• 1999

Regular exercise is effective in preventing coronary disease such as angina pectoris and infarction, inside it can lower the blood pressure and aids in weight control and release of stress. Risk factors of arteriosclerosis is hypertension, hyperlipidemia, diabetes, obesity, physical inactivity and excessive smoking. Arteriosclerosis begins at young age worsens with age, particulary in male. For people with risk factors of arteriosclerosis, it is important to prevent arteriosclerosis-related disease with dietary, living pattern and exercise prescription. Dietary fibers promote exercise of the digestive tract and shortens the time food remains inside the digestive tract. It can prevent obesity, hyperlipidemia, arteriosclerosis and colin cancer by blocking the absorption of cholesterol. Various vegetables and sea foods are lichen unsaturated fats and prevent the absorption of cholesterol inside the digestive tract. Essential fatty acids and unsaturated fats which are contained in vegetable oils, promotes metabolism while preventing absorption. In fruits, pectin water-soluble fiber, is present and lowers the level of cholesterol. By consuming foods that low in cholesterol and saturated fats, and rich in unsaturated fats, aliomentotherapy alone can reduce the plasma cholesterol by 10~l5$\%$. For ideal exercise, it should be aerobic with intensity of 60~80$\%$ HRmax, duration of 15~60min/day. The frequancy of 3~6/week is desirable the better exercise prescription is endurance aerobic exercise. To get more effect exercise, exercise consistency is very important.

• Nutrition Research and Practice
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• v.4 no.6
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• pp.522-527
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• 2010

Cardiovascular disease is a leading cause of death and disability and remains so in the future. The aim of this study was to detect the impact of a 6-month diet intervention on cardiovascular risk factors in postmenopausal Iranian women. It was a randomized controlled trial that carried out in the East Health Clinic in Ahvaz-Iran. This study started on June 2007 and was completed on May 2008. A total 64 healthy postmenopausal women recruited and randomly assigned to the intervention group (35) and control (29). Over the six months follow-up, the intervention group received five educational sessions (two face to face and three lecture discussion classes with slide demonstration) at the first month. These sessions were about menopause, cardiovascular disease and healthy diet. Every participant in the intervention group received one face to face education session at the $3^{rd}$ month, and also received a telephone call at the end of each month starting with the second month to remain on the diet. Pre-intervention and post-intervention anthropometric measurements, blood pressure, blood lipids and dietary intake were assessed. Data analyzed using the SPSS version 15. Descriptive (means and SD), univariate (paired-t test, Chi-Square and Independent T-test) and multivariate (GLM Repeated Measure) statistics were used. Participants in the diet group had significantly lower weight (-0.9 kg), body mass index (-0.4 $kg/m^2$), and fasting blood sugar (-4.5 mg/dl). The diet group significantly increased their daily intake of fiber (+2.3 g, P=0.05), decreased their intake of sodium (-28 mg, P=0.04), and consumption of fruit and vegetable ${\geq}$ 5 serving a day (80%, P=0.03) compared to the control group. Post menopausal women are at a greater risk for cardiovascular disease. Healthy diet using educational intervention can be an effective means of reducing cardiovascular risk in postmenopausal women.

• The Korean Journal of Mycology
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• v.14 no.2
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• pp.131-139
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• 1986

Cultural and nutritional conditions for Lyophyllum decastes and its chemical composition in a synthetic medium were investigated. The optimal temperature and pH for the production of mycelium were $25^{\circ}C$ and pH 7.5. The mycelium yield was the highest on 15th day. Among the carbon sources, glucose and CMC were the best for the production of mycelium and their optimal concentrations were 3 and 6%, respectively. As an organic nitrogen, proteose peptone was the best and $(NH_4)_2HPO_4$ as an inorganic nitrogen was good. The optimal concentration of proteose peptone and $(NH_4)_2HPO_4$ were 2 and 0.2%, respectively. The optimal ratio of glucose to proteose peptone for production of mycelium was 10 : 1. Also, the optimal concentrations of $K_2HPO_4$ and $MgSO_4$ were 0.2 and 0.06%, respectively and that of $CaCl_2$ was 0.1%. Among the bioextracts, yeast extract was the most effective and its optimal concentration was 1.5%. In chemical components of the mycelium of Lyophyllum decastes, total sugar, crude protein and crude fat were 34.80, 28.35 and 2.50%, respectively. Its ash was 7.57% and crude fiber 11.99%.

• Journal of Pharmaceutical Investigation
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• v.33 no.3
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• pp.171-178
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• 2003

Drug interactions with food, on occasion, lead to serious nutritional and functional changes in the body as well as alterations of pharmacological effect. It, therefore, should be necessary to take drug interactions with food into consideration for effective and safe therapeutics. Diabetes mellitus is a heterogeneous group of disorders characterzed by abnormal glucose homeostasis, resulting in hyperglycemia, and is associated with increased risk of microvascular, macrovascular, and neuropathic complications. However, the precise mechanism of diabetes mellitus remains unclear. Three basic objectives in the care of diabetic patients are maintaining optimal nutrition, avoiding hypo- or hyperglycemia and preventing complications. Laminaria japonica is a brown macroalgae which can be used as a functional diet due to high content of diatery fiber. The purpose of this study was to investigate the effect of Laminaria japonica diet on the pharmacokinetics of metformin which are frequently used in the treatment of diabetes. Diabetic rats induced by streptozotocin were employed in this study. Blood concentrations of oral hypoglycemic agent, metformin, were measured by HPLC and resultant pharmacokinetic parameters were calculated by RSTRIP. The mechanisms of drug interaction with food were evaluated on the basis of pharmacokinetic parameters such as $k_{a},\;t_{1/2},\;C_{max},\;t_{max}$, and AUC. Administration of metformin in normal and diabetic rats treated with Laminaria japonica diet showed significant decrease in AUC, $C_{max},\;and\;k_a$, and increase in $t_{max}$, compared to those with normal diet. This might result from adsortion of metformin on components of Laminaria japonica, causing delayed absorption. The oral glucose test showed that Laminaria japonica diet could lower blood glucose level probably through either inhibiting the activity of disaccharidases, intestinal digestive enzymes, or delaying the absorption of glucose. More studies should be followed to fully understand pharmacokinetic changes of metformin caused by long-term Laminaria japonica diet.

• The Journal of Korean Physical Therapy
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• v.17 no.4
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• pp.589-600
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• 2005

The purposes of this study is to discuss and analyze the effect on the recovery from cut in sciatic nerve. This study used 9 weeks male rats of Sprague-Dawley family. Rat in groups 4 were treated with pulsed therapeutic ultrasound for 3 minutes. 3 times weekly at 3MHz respectively (intensity; $0.2W/cm^2,\;0.5W/Cm^2,\;10W/cm^2$); rat in group 1 received placebo ultrasound. In addition, changes of serum aspartate amino-transferase(AST) and creatine phosphokinase(CPK) levels were also demonstrated with diameter of individual muscle fasciculate and number of muscle fiber in each of three types of muscles located in gastrocnemius, soles. The results of comparing the changes in groups are as follows; 1. We found out that hypertrophic epineurium was present in sciatic nerve injured ultrasound treatment of groups. 2. In the gastrocnemius morphological investigation of the group I (control group), severe muscle atrophy were observed at the 7th days of the sciatic nerve injury. however, muscle atrophy of the group IV ($1.0W/cm^2$) were slightly recovered at the 14th days after treatment ultrasound. At the 28th days, muscular fibers were formed in polygon and were significantly recovered. 3. C-fos immunoreactive of the group II ($0.2W/cm^2$), III ($0.5W/cm^2$) were remarkably increased at the 1th day after treatment of ultrasound. Group IV were markedly deceased. 4. Brain-Derived Neurotrophic Factor(BDNF) immunoreactive of the group II, III were increased after 7 days of the sciatic nerve injury. Group IV were markedly increased from 14th days to 28th days after treatment of ultrasound. 5. A significant increase of serum AST levels were demonstrated in control group. However, serum AST levels of massage groups were significantly decreased compared to that of control group in followed order ; ($0.2W/cm^2<0.5W/cm^2<1.0W/cm^2$). 6. A significant increase of serum CK levels were demonstrated in control of group. However, serum CK levels of massage groups were significantly decreased compared to that of control group in followed order ; ($0.2W/cm^2<0.5W/cm^2<1.0W/cm^2$). The above results suggest that ultrasound treatment after peripheral nerve injury might reduce noxious stimuli, facilitate nerve recovery and effective in the functional improvement delaying muscle atrophy or degeneration.

• Journal of the Korean Recycled Construction Resources Institute
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• v.5 no.1
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• pp.53-58
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• 2017

FRP Hybrid Bar, composed of an embedded steel and the coated composites with epoxy and glass fiber, is an effective construction material with tension-hardening performance and lightweight. The epoxy exposed to UV(Ultra Violet Rays) and FT(Freezing and Thawing) action easily shows a surface deterioration, which can cause degradation of bonding strength between inside-steel and outside-concrete. In the present work, surface inspection for 3 different samples of normal steel, FRP Hybrid Bar before UV, and FRP Hybrid Bar after UV test was performed, then concrete samples with 3 reinforcement types were prepared for accelerated FT test. Through visual inspection on 3 typed reinforcement, no significant deterioration like chalking was evaluated. The results from FT test to 120 and 180 cycles showed FRP Hybrid Bar exposed to UV test has higher bonding strength than normal steel by 106.3% due to enlarged bond area by silica coating. The 3 cases showed a similar bond strength tendency with increasing FT cycles, however a relatively big deviations of bond strength were evaluated in FRP Hybrid Bar after UV test due to loss of silica coating.