• Journal of Microbiology and Biotechnology
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• v.32 no.4
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• pp.437-446
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• 2022

In this study, to obtain icaritin with high pharmacological activities from icariin, which has a content ratio of over 58% in the total flavonoids of Epimedium herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in a medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30℃ for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the optimal pH and temperature were 5.0 and 40℃. The enzyme Km and Vmax values for icariin were 15.63 mM and 55.56 mM/h. Moreover, the enzyme hydrolyzed the 7-O-glucosides of icariin into icariside II, and finally hydrolyzed 3-Orhamnoside of icariside II into icaritin. The enzyme also hydrolyzed 7-O-glucosides of epimedin B to sagittatoside B, and then further hydrolyzed terminal 3-O-xyloside of sagittatoside B to icarisiede II, before finally hydrolyzing 3-O-rhamnoside of icarisiede II into icaritin. The enzyme only hydrolyzed 7-O-glucoside of epimedin A or epimedin C into sagittatoside A or sagittatoside C. It is possible to prepare icaritin from the high-content icariin in Epimedium herb using this enzyme. When 2.5% icariin was reacted at 40℃ for 18-20 h by the low-cost crude enzyme, 5.04 g icaritin with 98% purity was obtained from 10 g icariin. Also, the icaritin molar yield was 92.5%. Our results showed icaritin was successfully produced via cost-effective and relatively simple methods from icariin by crude enzyme. Our results should be very useful for the development of medicines from Epimedium herb.

• Journal of The Korean Association of Information Education
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• v.26 no.2
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• pp.141-151
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• 2022

Recently, basic literacy education related to digital literacy and data literacy has been emphasized for students who will live in a rapidly developing future digital society. Accordingly, demand for education to improve big data and data literacy is also increasing in general universities and universities of education as basic knowledge. Therefore, this study designed and applied big data analysis courses for pre-service teachers and analyzed the impact on data literacy. As a result of analyzing the interest and understanding of the input program, it was confirmed that it was an appropriate form for the level of pre-service teachers, and there was a significant improvement in competencies in all areas of 'knowledge', 'skills', and 'values and attitudes' of data literacy. It is hoped that the results of this study will contribute to enhancing the data literacy of students and pre-served teachers by helping with systematic data literacy educational research.

• The Journal of Asian Finance, Economics and Business
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• v.9 no.8
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• pp.19-27
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• 2022

Graduate well-being is foundational to academic success, and they are becoming more and more vulnerable. This is as they suffer from mental health challenges like anxiety and depression at rates six times higher than the general population. When the nature of their educational experience changes, such as when they had to stay in their homes during the COVID-19 pandemic, the stress on their mental health increases. The number of cases of emotional wellness among university students is considered a public health problem, but these young people often do not seek appropriate treatment. This study, therefore, aims to identify the influence of health behavior factors on graduate emotional wellness. This study used a questionnaire with a cross-sectional survey design. Questionnaires were distributed online to graduates from selected Private and Public Higher Education Institutions in Malaysia. The Partial Least Square Equation Model (PLS-SEM) was used to analyze the results of the study. Overall findings indicate that the health behavior factors have a significant influence on graduate emotional wellness. The findings from this study will benefit the management, academics, counselors, and other entities, including the Students' Representative Council, in identifying ways to improve services and upgrade the necessary facilities to enhance the graduate's emotional wellness.

• The Journal of the Korea Contents Association
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• v.22 no.6
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• pp.78-91
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• 2022

The importance of emotional intelligence education in the development of children's augmented reality education content is overlooked. Therefore, in-depth research is needed to develop children's emotional intelligence. This study was conducted through theoretical consideration and case analysis. The proposal of this paper is that the augmented reality type for children aged 2 to 7 is suitable for indoor activities with marking recognition technology. To promote an understanding of emotions, a large screen is selected, and emoticon dolls or emoticon books are recommended for learning content. Children aged 7 to 11 are suitable for indoor activities of non-marker recognition technology, and can induce emotional control and emotional recognition through active manipulation. For the learning content, "3D art teaching content" and "Online Classic Musical" are recommended. Children after the age of 11 are suitable for non-marker recognition technology outdoor activities and improve each element of emotional intelligence through interaction with nature and society. For the learning content, 'Forest Play Activity through Art' and 'EQ Theater Play' are recommended. Through this paper, we intend to promote the development of children's augmented reality emotional intelligence education.

• Journal of People, Plants, and Environment
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• v.23 no.1
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• pp.47-53
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• 2020

This study conducted a horticultural therapy program for trainees entrusted to the Juvenile Classification Review Center in order to fill the educational gap while providing emotional stability during the one-month period of commissioned education. The effects of horticultural therapy were examined by the pretest and posttest changes of the emotional items such as anxiety, self-esteem, self-efficacy and stress index. As the research method, this study used the one-group pretest-posttest experimental design on 16 female trainees of commissioned education staying at the Juvenile Classification Review Center in the juvenile reformatory located in A city. The horticultural therapy program was carried out in four sessions: two on flower arrangement and two on planting. The scores before and after the horticultural therapy program were measured using the Revised Children's Manifest Anxiety Scale (RCMAS) to examine the effects of horticultural therapy on the reduction of anxiety for the adolescents staying in the Juvenile Classification Review Center. The results show that there was significant decrease in the mean of anxiety from 62.5 (SD = 6.8) to 57.6 (SD = 8.1) points after the program (p = .002). There was no significant change in self-esteem, which was 76.9 (SD = 11.2) before the horticultural therapy and 78.3 (SD = 8.7) after the therapy (p = .420). In self-efficacy, there was no significant change from 72.9 (SD = 10.9) before and 75.1 (SD = 11.0) after horticultural therapy (p = .178). In order to examine the physiological changes in such emotional functions, this study measured the stress index using the uBioMacpa as the tool. The result of the measurement showed that there was a significant change in the mean from 33.8 (SD = 2.3) before to 31.1 (SD = 2.2) after the horticultural therapy (p < .001).

• Journal of Korea Society of Industrial Information Systems
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• v.27 no.4
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• pp.115-126
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• 2022

Under the COVID-19 pandemic, the importance of Official Development Assistance(ODA) in health and medical service is emphasized more. The number of cases is expected to increase in the future. Therefore, it is necessary to provide differentiated education and training for each type of occupation as a follow-up to realize the operations management and maintenance of medical equipment supported by the ODA project. In this study, for an effective follow-up and operations management of medical equipment supported by the ODA project, the differences in educational training achievement between users and maintainers were analyzed through a t-test. As a result of this study, there were significant differences between users and maintainers of medical equipment in use and manage six types of priority management target medical equipment(Q5) and an instruction manual understanding of medical equipment(Q6). However, there was no difference in the need and purpose of education and training, satisfaction with an instructor, and the need for cross-professional communication.

• Journal of The Korean Association of Information Education
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• v.26 no.1
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• pp.1-10
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• 2022

With the development of artificial intelligence, society is moving toward another world that has never existed before. As a result, interest in artificial intelligence education is also increasing, and research on artificial intelligence education is being conducted more actively in Korea. However, many studies have been conducted focusing on the upper grades of elementary school, and curriculum and programs for the lower grades are still insufficient. Therefore, in this study, a total of 6 sessions of artificial intelligence programs were developed to understand image recognition for the lower grades of elementary school. The validity was secured by conducting expert validity for 8 experts, and the effectiveness was verified through the pre-post-response sample t-test by applying it to the experimental group. As a result, both artificial intelligence understanding and artificial intelligence attitude showed statistically significant results, and both the interest and difficulty of educational programs were found to be suitable for lower grade students. Based on the contents of this study, it is necessary to review its application and effectiveness in various environments through follow-up studies in the future.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.447-456
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• 2022

The present study was carried out to investigate the effect of Arctigenin on cell growth and the mechanism of cell death elicited by Arctigenin were examined in FaDu human pharyngeal carcinoma cells. To determine the apoptotic activity of Arctigenin in FaDu human pharyngeal carcinoma cells, cell viability assay, DAPI staining, caspase activation analysis, and immunoblotting were performed. Arctigenin inhibited the growth of cells in a dose-dependent manner and induced nuclear condensation and fragmentation. Arctigenin-treated cells showed caspase-3/7 activation and increased apoptosis versus control cells. FasL, a death ligand associated with extrinsic apoptotic signaling pathways, was up-regulated by Arctigenin treatment. Moreover, caspase-8, a part of the extrinsic apoptotic pathway, was activated by Arctigenin treatments. Expressions of anti-apoptotic factors such as Bcl-2 and Bcl-xL, components of the mitochondria-dependent intrinsic apoptosis pathway, significantly decreased following Arctigenin treatment. The expressions of pro-apoptotic factors such as BAX, BAD and caspase-9, and tumor suppressor -53 increased by Arctigenin treatments. In addition, Arctigenin activated caspase-3 and poly (ADP-ribose) polymerase (PARP) induced cell death. Arctigenin also inhibited the proliferation of FaDu cells by the suppression of p38, NF-κB, and Akt signaling pathways. These results suggest that Arctigenin may inhibit cell proliferation and induce apoptotic cell death in FaDu human pharyngeal carcinoma cells through both the mitochondria-mediated intrinsic pathway and the death receptor-mediated extrinsic pathway.

• Journal of Korean Academy of Nursing
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• v.52 no.5
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• pp.535-549
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• 2022

Purpose: The study aimed to design and develop an automated personalized self-care (APSC) program for patients with type 2 diabetes mellitus. The secondary aim was to present a clinical protocol as a mixed-method research to test the program effects. Methods: The APSC program was developed in the order of analysis, design, implementation, and evaluation according to the software development life cycle, and was guided by the self-regulatory theory. The content validity, heuristics, and usability of the program were verified by experts and patients with type 2 diabetes mellitus. Results: The APSC program was developed based on goal setting, education, monitoring, and feedback components corresponding to the phases of forethought, performance/volitional control, and self-reflection of self-regulatory theory. Using the mobile application, the participants are able to learn from educational materials, monitor their health behaviors, receive weekly-automated personalized goals and feedback messages, and use an automated conversation system to solve the problems related to self-care. The ongoing two-year study utilizes a mixed method design, with 180 patients having type 2 diabetes mellitus randomized to receive either the intervention or usual care. The participants will be reviewed for self-care self-efficacy, health behaviors, and health outcomes at 6, 12, 18, and 24 months. Participants in the intervention group will be interviewed about their experiences. Conclusion: The APSC program can serve as an effective tool for facilitating diabetes health behaviors by improving patients' self-care self-efficacy and self-regulation for self-care. However, the clinical effectiveness of this program requires further investigation.

• International Journal of Computer Science & Network Security
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• v.21 no.7
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• pp.211-220
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• 2021

The aim of the study is to implement a factor analysis of the determinants of pricing in a state-regulated competitive market using economic and mathematical modelling methods and to develop ways to improve the pricing environment of the market under study. The purpose of the work defines the main objectives: (i) to investigate the features of the competitive model of the Ukrainian flour market; (ii) to analyse the current price conjuncture of the flour market and the dynamics of the main determinants of pricing; (iii)to develop ways of improving the price situation on the flour market on the basis of the factor analysis on the results of economic and mathematical modelling. In order to ensure the reliability and validity of the research results, the following methods were applied: the logical-dialectical method of scientific knowledge in the study of the main theoretical aspects of flour market functioning, the method of logical generalisation and synthesis, comparison, factor analysis, correlation and regression analysis, the graphical method, etc. It has been shown that pricing in a state-regulated competitive market has its own characteristics. For example, in the flour market the price of goods cannot be influenced by producers (sellers) by any methods, therefore determinants of pricing by indirect influence have been taken into account. The five-factor power model of wheat flour price has been constructed. It was substantiated that the price of wheat flour in Ukraine is mostly influenced by consumer price index (0.92 %). The received complex model of wheat flour price may be used also for medium-term forecasting and working out the ways of price formation optimization in the flour market.