• Animal cells and systems
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• v.15 no.1
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• pp.37-43
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• 2011

The Korean freshwater crayfish, Cambaroides similis, has recently suffered from range reduction and habitat degradation caused by environmental changes and water pollution. For the conservation and restoration of this species, it is necessary to understand the current population structures of Korean C. similis using estimation of their genetic variation. In this study, eight micro satellite loci were developed and characterized from 49 individuals collected from four locations: one population from Mt. Bukhan (BH) and three populations from Mt. Gwanak (GA) in Seoul, Korea. As a result, the number of alleles per locus ranged from 2 to 12. The observed heterozygosities and expected heterozygosities ranged from 0.000 to 0.833 and from 0.125 to 0.943, respectively, and the former values were significantly lower than the latter ones expected under the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was revealed between any of the locus pairs after Bonferroni correction. From the pairwise Fst results over all samples, higher differentiation between GA-BH population pairs (mean 0.1789) was observed than between GA population pairs (mean 0.0454). This was also supported by Mantel's test showing that the genetic distances of these crayfish populations were significantly correlated with geographic distances. This result may show the regional differentiation caused by restricted gene flow between northern (BH) and southern (GA) populations within Seoul. These micro satellite markers have the potential for use in analyses of the genetic diversity and population structure of C. similis species, with implications for its conservation and management plans.

• Journal of Fashion Business
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• v.18 no.5
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• pp.25-41
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• 2014

As the smart phone market grows rapidly, the significance of the application (app) market also increases. The purposes of this study were to identify market segments according to technological innovativeness and clothing involvement, and to compare the differences in lifestyles, benefits of fashion information app sought, and intention to use. The data were collected from smart phone users in their 10s to 30s in 2013, and a total of 292 data sets were analyzed. The findings of this study were as follows. The respondents were divided into three groups, innovative group, clothing involvement group, and passive group. Significant differences were found among three segments in terms of age, marital status, education level, and monthly income. With respect to five lifestyle factors, innovative group and clothing involvement group had higher mean scores for rational purchase, personal relationship, and personality pursuit than passive group. However, these two groups showed differences in brand pursuit factor. The benefits of the fashion information app were generated into two factors, information benefit and enjoyment benefit. Innovative group and clothing involvement group had higher values than passive group in these two factors. Finally, innovative group had the highest value on the intention to use fashion information app. This study attempts to provide fundamental information about the potential market segment for managers or marketers developing fashion information apps.

• Proceedings of the KSRS Conference
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• 2009.03a
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• pp.354-359
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• 2009

Land cover types of Hustai National Park (HNP) in Mongolia, a hotspot area with rare species, were classified and their temporal changes were evaluated using Landsat MSS TM/ETM data between 1994 and 2000. Maximum likelihood classification analysis showed an overall accuracy of 88.0% and 85.0% for the 1994 and 2000 images, respectively. Kappa coefficients associated with the classification were resulted to 0.85 for 1994 and 0.82 for 2000 image. Land cover types revealed significant temporal changes in the classification maps between 1994 and 2000. The area has increased considerably by $166.5km^2$ for mountain steppe. By contrast, agricultural areas and degraded areas affected by human being activity were decreased by $46.1km^2$ and $194.8km^2$ over the six year span, respectively. These areas were replaced by mountain steppe area. Specifically, forest area was noticeably fragmented, accompanied by the decrease of $\sim400$ ha. The forest area revealed a pattern with systematic gain and loss associated with the specific phenomenon called as forest free-south slope. We discussed the potential environmental conditions responsible for the systematic pattern and addressed other biological impacts by outbreaks of forest pests and ungulates.

• Research in Community and Public Health Nursing
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• v.12 no.1
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• pp.49-59
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• 2001

Purpose: This study was intended to develop the framework of public health center based 'Child growth and developmental clinic' and analyze the efficiency of services. This was the part of Mother-Child Health Guidance Project by the Public Health Center in Seoul. The purposes of this project were to improve children's health and development with early detection of developmental delay by screening test, and to assist and guide the parent for child care and rearing, so that children can reach their maximum potential. Method: Framework and standards for operation of 'Child growth and development clinic' were developed. We selected screening tool. developed parent education materials, and educated the personnel of public health center. Result: 243 children were enrolled for developmental screening, and we detect the children with developmental delay and referred them for further evaluation. The proportion of developmental delayed children was approximately 2% of the enrolled children. Also, we analyzed the characteristics of enrolled children, and their parental needs and attitude on child rearing. Some problems in operation of developmental clinic were discussed. Conclusion: Though we can found some limitations, we are confident that pubic health center can be charge of important role for improving child development for the majority. This study will be the basis of children's developmental clinic running by public health center.

• Biomaterials and Biomechanics in Bioengineering
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• v.3 no.2
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• pp.105-114
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• 2016

The purpose of this study is to evaluate the effects of cryopreservation on dental pulp-derived stem cells (DPSC) viability over a period of three years. Dental pulp-derived stem cells were isolated and cultured from thirty-one healthy teeth. DPSC isolates were assessed for doubling-time and baseline viability prior to cryopreservation and were assessed again at three time points; one week (T1), 18 months (T2), and 36 months (T3). DPSC can be grouped based on their observed doubling times; slow (sDT), intermediate (iDT), and rapid (rDT). Viability results demonstrated all three types of DPSC isolates (sDT, iDT and rDT) exhibit time-dependent reductions in viability following cryopreservation, with the greatest reduction observed among sDT-DPSCs and the smallest observed among the rDT-DPSC isolates. Cryopreserved DPSCs demonstrate time-dependent reductions in cellular viability. Although reductions in viability were smallest at the initial time point (T1) and greatest at the final time point (T3), these changes were markedly different among DPSC isolates with similar doubling times (DTs). Furthermore, the analysis of various DPSC biomarkers - including both intracellular and cell surface markers, revealed differential mRNA expression. More specifically, the relative high expression of Sox-2 was only found only among the rDT isolates, which was associated with the smallest reduction in viability over time. The expression of Oct4 and NANOG were also higher among rDT isolates, however, expression was comparatively lower among the sDT isolates that had the highest reduction in cellular viability over the course of this study. These data may suggest that some biomarkers, including Sox-2, Oct4 and NANOG may have some potential for use as biomarkers that may be associated with either higher or lower cellular viability over long-term storage applications although more research will be needed to confirm these findings.

• Journal of Sensor Science and Technology
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• v.7 no.4
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• pp.271-278
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• 1998

A ISFET-based glucose sensor has inherent problems such as low sensitivity, drift effect and long response time. For that reason, a amperometric actuation technique was introduce to make a highly sensitivity of the ISFET glucose sensor with a Pt actuator, which electrolyzes $H_2O_2$, one of the by a by-products of the oxidation reaction of glucose. Moreover, a potential-step measurement method detecting response by only the electrolysis of $H_2O_2$ was developed for eliminating a drift problem. The operation characteristics of ISFET-based glucose sensor was improved by using the amperometric actuation and a measurement techniques. The fabricated ISFET glucose sensor is shown good operation such as characteristics(30mM PBS, about 26mV/decade) and linearity. A portable glucose meter with a highly resolution by using the fabricated ISFET-based glucose sensor with Pt actuation was developed and its characteristics investigated.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.49-58
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• 2009

This study was done to provide a case model with a concept of environmental integration based on the water circulation system. Area of interest (AOI) is the Eastern Pangyo New Town area, which has several advantages in adaptation of a water circulation system. The AOI has a potential of maintaining water resources, and has a good condition to construct the water circulation system. Research done for the purpose of the establishment of the water circulation system in the Eastern Pangyo New Town shows the following. The main sources of water supply in the water circulation system in the Eastern Pangyo New Town is from two subway stations and runoff water, along with the natural water flowing from the mountains, rain water, and stream water. It was determined that more than 35,000 tons of water would be needed for the creation of water circulation system at the Eastern Pangyo. If the creation of infrastructure for the use of runoff and rain water as well as the periodic management can be provided, it can serve as the new model for a new city with water circulation system. In addition, since the Eastern Pangyo New Town water circulation system can secure enough amount of water resources, natural drainage system (NDS) in which it can be in dry condition in non-rainy days, is applied and connected to the typical waterways. Such water circulation system has many positive aspects including the wise use of water resources, and providing wild Life animals corridors and habitats. Also, the water circulation system can lead to the environmental education to the residents and visitors on environmental awareness of the water circulation system and their environment.

• Broadcasting and Media Magazine
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• v.11 no.4
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• pp.118-131
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• 2006

With rapid development in ultra fast communication and digital multimedia, the realistic broadcasting technology, that can stimulate five human senses beyond the conventional audio-visual service is emerging as a new generation broadcasting technology. In this paper, we introduce a haptic broadcasting system and related core system and component techniques by which we can 'touch and feel' objects in an audio-visual scene. The system is composed of haptic media acquisition and creation, contents authoring, in the haptic broadcasting, the haptic media can be 3-D geometry, dynamic properties, haptic surface properties, movement, tactile information to enable active touch and manipulation and passive movement following and tactile effects. In the proposed system, active haptic exploration and manipulation of a 3-D mesh, active haptic exploration of depth video, passive kinesthetic interaction, and passive tactile interaction can be provided as potential haptic interaction scenarios and a home shopping, a movie with tactile effects, and conducting education scenarios are produced to show the feasibility of the proposed system.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.123-129
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• 2014

The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2'-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC ($1.9172{\mu}g/mL$) > OSC ($1.5218{\mu}g/mL$) > CPR ($1.0611{\mu}g/mL$) > OSR ($0.6782{\mu}g/mL$). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.

• Journal of the Korean Society of Safety
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• v.23 no.6
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• pp.14-20
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• 2008

A large number of accidents have been occurred in the domestic shipbuilding industry. Its number of accidents is approximately twice than that of manufacturing industry and three times larger than that of whole industry in general. There are several reasons that make it hard to control the accidents. First, it is because the shipbuilding industry is composed of many subcontractors who may need improvement in safety education and safety management. Second, the shipyard environments are exposed to lots of hazards such as high elevation works, isolated workspaces, heavyweight and huge structures/components. Last, the shipbuilding industry has grown too rapidly in a short period. For that reason, the safety management standards and safety consciousness could not catch up the speed of its development. In this study, it is suggested to use a more efficient and easier way to conduct safety management using a web-based safety management system for the shipbuilding industry. This system is composed of four main parts, which is available for self-auditing with legal support. Those are checklists based safety and health evaluation, safety inspection, user shared accident database and user shared bulletin board. It is expected that this system can help us to reduce risks in the shipbuilding industry considerably.