• Animal Bioscience
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• v.37 no.6
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• pp.1001-1006
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• 2024

Objective: This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4℃. Methods: Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1,000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4℃ for 24, 72, 120, and 168 h. Sperm acrosome integrity rate was analyzed by Giemsa staining at 24, 72, and 120 h, and mitochondrial membrane integrity was analyzed by Mito-Tracker Red CMXRos. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of spermatozoa were measured after 120 h of preservation. Results: The sperm viability and forward-moving sperm under 200 mg/L CPP were significantly higher than that in the control group at 72 h (61.28%±3.89% vs 52.83%±0.70%, 51.53%±4.06% vs 42.84%±1.14%), and 168 h (47.21%±0.85% vs 41.43%±0.37%, 38.68%±0.87% vs 31.68%±0.89%). The percentage of fast-moving sperm (15.03%±1.10% vs 11.39%±1.03%) and slow-moving sperm (23.63%±0.76% vs 20.29%±1.11%) in the 200 mg/L group was significantly higher than control group at 168 h. The mitochondrial membrane integrity of the sperm in the group with 200 mg/L CPP was significantly higher than those in the control group after storage at 4℃ for 120 h (74.76%±2.54% vs 65.67%±4.51%, p<0.05). The acrosome integrity rate in the group with 200 mg/L (87.66%±1.26%) and 400 mg/L (84.00%±2.95%) was significantly higher than those in the control group (80.65%±0.16%) after storage for 24 h (p<0.05). CPP also increased T-AOC and decreased the MDA concentration after preservation at 4℃ (p<0.05). Conclusion: Adding CPP could improve the T-AOC of sperm, inhibit lipid peroxidation, and facilitate semen preservation.

• International Journal of Stem Cells
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• v.15 no.3
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• pp.247-257
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• 2022

Background and Objectives: Although human-induced pluripotent stem cells (hiPSC) can be efficiently differentiated into cardiomyocytes (CMs), the heterogeneity of the hiPSC-CMs hampers their applications in research and regenerative medicine. Retinoic acid (RA)-mediated signaling pathway has been proved indispensable in cardiac development and differentiation of hiPSC toward atrial CMs. This study was aimed to test whether RA signaling pathway can be manipulated to direct the differentiation into sinoatrial node (SAN) CMs. Methods and Results: Using the well-characterized GiWi protocol that cardiomyocytes are generated from hiPSC via temporal modulation of Wnt signaling pathway by small molecules, RA signaling pathway was manipulated during the differentiation of hiPSC-CMs on day 5 post-differentiation, a crucial time point equivalent to the transition from cardiac mesoderm to cardiac progenitor cells in cardiac development. The resultant CMs were characterized at mRNA, protein and electrophysiology levels by a combination of qPCR, immunofluorescence, flow cytometry, and whole-cell patch clamp. The results showed that activation of the RA signaling pathway biased the differentiation of atrial CMs, whereas inhibition of the signaling pathway biased the differentiation of sinoatrial node-like cells (SANLCs). Conclusions: Our study not only provides a novel and simple strategy to enrich SANLCs but also improves our understanding of the importance of RA signaling in the differentiation of hiPSC-CMs.

• Korean Journal of Organic Agriculture
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• v.27 no.3
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• pp.307-325
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• 2019

This study aims to analyze the regional variations in which appear during the formation process of educational community in rural villages from the perspective of regional innovation theory. The study results revealed that the activities to form an educational community of the Songak village significantly influenced the variations across the social, economic and environmental aspects beyond an innovation of public education within the region based on interrelation between schools and villages. In other words, the activities including interactions and group learnings have been actively promoted among the various relevant stakeholders, thereby resulting in a variety of positive variations such as expanded regional welfare base, activated voluntary residents community gatherings based on the unity and cooperation, broadened socioeconomic opportunities, satisfaction on educational and cultural desires, and construction of healthy ecological environment in the region. Therefore, it is thought that regional development policy suitable for each region needs to be established by embracing the fact that activities to form a village educational community entail significant implication and value as the comprehensive regional innovation activities across the entire domains in each region.

• The Korean Journal of Health Service Management
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• v.12 no.1
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• pp.1-11
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• 2018

Objectives : The purpose of this study was to identify the relationship between decision-making types, public entrepreneurship, and performance management of local public hospital directors. Methods : A questionnaire survey was carried out to assess the dependent variables of directors' decision-making types and public entrepreneurship. The analysis of management performance was carried out through a comparison between 2016 results of, data of variation rate on medical revenue and change rate on medical profit and results in 2015. Results : Results indicated that local public hospital directors who used rational decision-making showed better performance management. The analysis showed that enterprise had a greater positive effect (+) on variation rate of medical revenue than that of innovation. However, innovation had a higher positive effect (+) on change rate of medical profit than that of enterprise. These results suggest that innovation and enterprise have a major influence on performance management. Conclusions : The survey used for this study suggests that an education and training program is needed to improve public hospital directors' ability for rational decision-making, public entrepreneurship and performance management. Additionally, the policy change guaranteeing autonomy within the proper range is demanded that Local Public Hospital Director having spirit of innovation and enterprise achieves peak capacity and have responsibility for management.

• The Journal of the Convergence on Culture Technology
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• v.4 no.2
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• pp.61-67
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• 2018

The purpose of this paper is to explore the educational direction and present improvements of the local college of engineering through the business feasibility and analysis of the universities that conducted financial support projects to improve engineering education. For this study, we analyzed a three-year of survey on the business satisfaction of the university that implemented aid for innovation in engineering education. A result of statistical analysis on the performance of the education programs and operations that performed by the participants in the project shows that the overall satisfaction rate of the support projects has increased from 73.8% to 83.4%, but the average rate of the related industries has relatively decreased. The research shows that the satisfaction level of creativity and problem-solving ability of new employees in task performance was low so that the overall satisfaction level of the business decreased. We are going to come up with improvement plan to develop creative problem solving skills through the design of program for engineering education innovation in the future.

• Journal of Korea Technology Innovation Society
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• v.13 no.3
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• pp.423-445
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• 2010

This study purposes to suggest a next generation research and education environment which is possible by the linkage of two sectors based on cyberinfrastructure. It is to design a new research-education linkage system which can apply the results of R&D to the higher education in natural science and engineering areas. literatures were reviewed on new paradigms of R&D triggered by the advent of cyberinfrastrcuture. A couple of overseas and domestic cases of such paradigm were introduced. With the backgrounds of case studies and theoretical models, a new research-education linkage environment system was suggested. Specially e-AIRS system was introduced as an example of new model in Korea. In addition, some policy issues were discussed as related to the successful promotion of such programs.

• Journal of Service Research and Studies
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• v.8 no.4
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• pp.1-12
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• 2018

This study suggests a way to implement shared economic model of educational service in service era. As most shared economic models are innovating high-cost, low-quality structures in existing economies, there is a need for innovation models that improve high-cost low-quality structures to high-quality, low-cost structures in education services. Especially, since higher education and lifelong education are in urgent need of innovation, this study studied shared university model focused on higher education and lifelong education. In order to provide educational services at low cost and high quality, it is necessary to use idle resources, so it is necessary to introduce a shared economic model. Shared universities require a more efficient and effective supplier quality control, as the existing limited supplier group become widespread. Normative quality control is also needed to cultivate students as competent talent. In addition, in order to provide high-quality higher education services at a low cost, it is necessary to base the process quality management using advanced science and technology. Therefore, educational service quality management is a process of quality control that is well-balanced in terms of suppliers, customers, and processes.

• Journal of Engineering Education Research
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• v.18 no.5
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• pp.22-31
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• 2015

The purpose of this study is to suggest the general education contents for undergraduate students at engineering related departments through need analysis in order to develop creative and integral talents. For this study, the need analysis was conducted by using focus group interviews, Borich's educational need equation and the Locus for Focus Model, and highly ranked priorities on both methods proposed as the highest priorities. As a result, 'drawing up a letter of self-introduction and a portfolio', 'understanding fields', 'understanding careers related to the major', 'presentation skills', 'knowledge for certificated exams', 'method to deduct creative ideas' were high on the list of top five priorities. The selected items by Locus for Focus Model show that undergraduate students at engineering related departments need general education contents associated with career development and communication by priority.

• Journal of Engineering Education Research
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• v.16 no.2
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• pp.37-49
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• 2013

In this paper, articles in full body of JEER in Korea and JEE in U.S. were classified into two categories- Research area and Key words-to endeavor a comparative study of the engineering education research(EER) topics and their temporal changes. In the recent 4 year term, 'Accreditation of Engineering Education' and 'Courses & Curriculum' were the most common key words appearing in JEER and 'Researches on EER' and 'Learner's Factor' in JEE. If we put curriculum/teaching materials/teaching as the input elements and learning/assessment as the output elements for education achievement, JEE demonstrates that many of the research topics have shifted from input to output in the recent years, while JEER research is in the transient stage.

• Journal of Engineering Education Research
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• v.21 no.3
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• pp.46-55
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• 2018

The purpose of this study was to develop the character index instrument for engineering college students. For this, literature review and survey were used. The survey was conducted with 275 engineering college students in D university. Finally, draft of character index instrument for engineering college students was developed with 10 factors and 47 questions. Based on the results, the investigation of engineering college students' needs is necessary to conduct for developing character education programs. And it is necessary to research validity of the draft of character index instrument for engineering college students. It is necessary to develop various convergence subjects by experiencing the character substantially for engineering college students.