• 방송공학회논문지
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• 제20권4호
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• pp.619-631
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• 2015

본 논문에서는 방송 콘텐츠와 개인 콘텐츠를 편집하기 위한 새로운 온라인 콘텐츠 편집 시스템을 제안한다. 제안하는 편집 시스템은 콘텐츠를 저장하고 관리하기 위한 콘텐츠 관리 서버와 사용자 인터페이스를 통해 콘텐츠 편집을 수행하는 콘텐츠 편집기로 구성된다. 편집 대상 콘텐츠를 다운로드 받아 편집을 수행하는 기존의 콘텐츠 편집 방법과는 달리, 제안하는 편집 시스템에서는 콘텐츠 관리 서버에 저장되어 있는 콘텐츠를 콘텐츠 편집기에서 스트리밍 기술을 이용하여 재생하며 편집을 수행한다. 그러나 콘텐츠 편집기가 스트리밍 기술을 이용하여 콘텐츠의 전체 구간을 재생하며 편집을 수행하는 것은 비효율적이다. 이러한 문제점을 해결하기 위해, 제안하는 편집 시스템에서는 편집 대상 콘텐츠의 씬 검출을 수행한 후, 검출된 씬 정보를 콘텐츠 재생 및 편집에 사용한다. 콘텐츠 편집기를 통해 콘텐츠 편집이 완료되면, 편집 정보는 메타데이터 형태로 콘텐츠 관리서버에 업로드된다. 제안하는 편집 시스템에서는 콘텐츠의 씬 정보와 편집 정보가 메타데이터 형태로만 존재하며, 씬 단위 혹은 편집 분절 단위의 물리적인 콘텐츠 분할은 수행되지 않는다. 구현 결과는 제안한 편집 시스템이 편집 대상 콘텐츠를 다운로드한 후 편집하는 기존의 콘텐츠 편집 방법을 사용하지 않고서도 기존의 방법과 유사한 성능을 제공함을 보여준다.

• 한국멀티미디어학회논문지
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• 제16권8호
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• pp.972-981
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• 2013

비선형 편집(Non-linear Editing)이란 전통적인 선형 편집(Linear Editing) 방법과 달리 편집자가 원하는 대로 프레임 사이에 새로운 프레임을 삽입할 수 있는 편집 방법이다. 비선형 편집 시스템이란 이러한 편집방법을 지원하는 시스템으로 하나의 컴퓨터와 편집 소프트웨어 그리고 영상의 입 출력을 담당하는 캡처보드(Capture Board)로 구성된다. 현재 TV방송과 영화제작 과정에서는 이러한 비선형 편집 시스템을 사용하고 있다. 촬영된 영상이나 다양한 그래픽 소스는 비선형 편집 시스템을 통하여 하나의 영상콘텐츠로 제작된다. 본 논문에서는 많은 편집자들이 사용하고 있는 어도비 프리미어(Adobe Premiere)를 중심으로 비선형 편집 소프트웨어의 기능과 영상 편집 과정에서의 장 단점을 살펴보고 앞으로의 비선형 편집 소프트웨어의 개선 방향에 대하여 논의한다.

• 한국인쇄학회지
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• 제13권1호
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• pp.57-72
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• 1995

The function and technological merit of electronic image editing system using time-code addressing method are investigated. The method shortens the editing time and gives high accuracy. It is possible to digitize the editing system for high resolution and to vary and to systemize the VTR editing technique by this method.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1522-1529
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• 2024

CRISPR-Cas system is being used as a powerful genome editing tool with developments focused on enhancing its efficiency and accuracy. Recently, the miniature CRISPR-Cas12f1 system, which is small enough to be easily loaded onto various vectors for cellular delivery, has gained attention. In this study, we explored the influence of temperature conditions on multiplex genome editing using CRISPR-Cas12f1 in an Escherichia coli model. It was revealed that when two distinct targets in the genome are edited simultaneously, the editing efficiency can be enhanced by allowing cells to recover at a reduced temperature during the editing process. Additionally, employing 3'-end truncated sgRNAs facilitated the simultaneous single-nucleotide level editing of three targets. Our results underscore the potential of optimizing recovery temperature and sgRNA design protocols in developing more effective and precise strategies for multiplex genome editing across various organisms.

• 공학논문집
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• 제3권1호
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• pp.21-27
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• 1998

본 논문에서는 SGML DTD(Document Type Definition)의 문서 구조정보를 이용하여 SGML 실례문서를 편집하기 위한 시스템을 설계 및 구현하였다. 이를 위해 문서의 논리구조 표현을 위한 구조 창을 이용하여 SGML 문서를 편집할 수 있어 SGML에 대해 모르는 사용자도 편집오류 없이 문서를 생성할 수 있고 엘리먼트(element)와 속성(attribute), 엔티티(entity)를 지원하는 도구를 이용하여 엘리먼트 등을 손쉽게 수정 가능하고, 생성된 문서를 SGML 파서(parser)를 이용하여 검증할 수 있도록 시스템을 설계하였다. 또한 본 시스템은KS 5601코드를 사용하여 한글과 영문 텍스트를 모두 지원한다. 본 논문에서 설계한 SGML 문서 편집 시스템은 윈도우 사용자 인터페이스를 위해 윈도우95 시스템 환경 하에서 구현하였다.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.903-911
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• 2021

Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.194-194
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.200-200
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• International Journal of Stem Cells
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• 제17권1호
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• pp.1-14
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• 2024

The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

• Clinical and Experimental Pediatrics
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• 제64권6호
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• pp.269-279
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• 2021

Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) is an ancient prokaryotic defense system that precisely cuts foreign genomic DNA under the control of a small number of guide RNAs. The CRISPR-Cas9 system facilitates efficient double-stranded DNA cleavage that has been recently adopted for genome editing to create or correct inherited genetic mutations causing disease. Congenital heart disease (CHD) is generally caused by genetic mutations such as base substitutions, deletions, and insertions, which result in diverse developmental defects and remains a leading cause of birth defects. Pediatric CHD patients exhibit a spectrum of cardiac abnormalities such as septal defects, valvular defects, and abnormal chamber development. CHD onset occurs during the prenatal period and often results in early lethality during childhood. Because CRISPR-Cas9-based genome editing technology has gained considerable attention for its potential to prevent and treat diseases, we will review the CRISPR-Cas9 system as a genome editing tool and focus on its therapeutic application for CHD.