• Journal of Broadcast Engineering
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• v.20 no.4
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• pp.619-631
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• 2015

In this paper, we propose a new online content editing system to edit the broadcasting and personal contents. The proposed editing system consists of the content management server and the content editor, in which the content management server is used for storing the contents, while the content editor is used for editing the contents via the user interface. Unlike the existing content editing methods which edit the downloaded contents, the proposed editing system edits the contents stored in the content management server while the content editor plays them by using the streaming technology. However, it is not effective to edit the whole segments of contents while playing them by using the streaming technology. To resolve the problem, the proposed editing system performs the scene detection of the contents, and the detected scene information is used for playing and editing of contents. After completing the content editing, the edited information is uploaded to the content management server as a metadata format. In the proposed editing system, both scene and edited information are represented only as the metadata format, and the physical content segmentation according to the scene and edited information is not performed. The implementation results show that the proposed editing system provides similar performances with the existing content editing methods which use the content download and editing methods.

• Journal of Korea Multimedia Society
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• v.16 no.8
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• pp.972-981
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• 2013

Non-linear editing is an editing method that, unlike linear editing, enables users to insert a new frame between existing frames. Non-linear editing systems are systems that support such method, and are composed of a computer, a software and a capture board that inputs/outputs video signals. Non-linear editing is used for modern television broadcasts and movie productions. Recorded footage and various graphic sources are combined into one visual content through a non-linear editing system. In this paper, we will look into functions of various non-linear editing softwares (mainly the most common of all; Adobe Premiere) as well as their merits and demerits during the editing process, and will also discuss their future Improvement direction.

• Journal of the Korean Graphic Arts Communication Society
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• v.13 no.1
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• pp.57-72
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• 1995

The function and technological merit of electronic image editing system using time-code addressing method are investigated. The method shortens the editing time and gives high accuracy. It is possible to digitize the editing system for high resolution and to vary and to systemize the VTR editing technique by this method.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1522-1529
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• 2024

CRISPR-Cas system is being used as a powerful genome editing tool with developments focused on enhancing its efficiency and accuracy. Recently, the miniature CRISPR-Cas12f1 system, which is small enough to be easily loaded onto various vectors for cellular delivery, has gained attention. In this study, we explored the influence of temperature conditions on multiplex genome editing using CRISPR-Cas12f1 in an Escherichia coli model. It was revealed that when two distinct targets in the genome are edited simultaneously, the editing efficiency can be enhanced by allowing cells to recover at a reduced temperature during the editing process. Additionally, employing 3'-end truncated sgRNAs facilitated the simultaneous single-nucleotide level editing of three targets. Our results underscore the potential of optimizing recovery temperature and sgRNA design protocols in developing more effective and precise strategies for multiplex genome editing across various organisms.

• The Journal of Engineering Research
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• v.3 no.1
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• pp.21-27
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• 1998

This paper describes the design and implementation of system for editing SGML document instance using document structure information of SGML DTD. For make use of structure window for logical structure expression of document to SGML document editing without editing mistake of user and easy update the using support to editing process of elements, attributes, entities tools and product document, and valid using SGML parser. Also, in order to support Korean and English text using KS 5601. In this paper, the proposed SGML document editing system is used common controls support of window 95 for window user interface

• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.903-911
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• 2021

Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.194-194
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.200-200
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• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• International Journal of Stem Cells
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• v.17 no.1
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• pp.1-14
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• 2024

The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

• Clinical and Experimental Pediatrics
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• v.64 no.6
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• pp.269-279
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• 2021

Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) is an ancient prokaryotic defense system that precisely cuts foreign genomic DNA under the control of a small number of guide RNAs. The CRISPR-Cas9 system facilitates efficient double-stranded DNA cleavage that has been recently adopted for genome editing to create or correct inherited genetic mutations causing disease. Congenital heart disease (CHD) is generally caused by genetic mutations such as base substitutions, deletions, and insertions, which result in diverse developmental defects and remains a leading cause of birth defects. Pediatric CHD patients exhibit a spectrum of cardiac abnormalities such as septal defects, valvular defects, and abnormal chamber development. CHD onset occurs during the prenatal period and often results in early lethality during childhood. Because CRISPR-Cas9-based genome editing technology has gained considerable attention for its potential to prevent and treat diseases, we will review the CRISPR-Cas9 system as a genome editing tool and focus on its therapeutic application for CHD.