• 한국응용곤충학회지
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• 제48권2호
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• pp.237-243
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• 2009

양배추 유묘에서 도둑나방 유충의 경제적 피해허용수준과 요방제 수준을 설정하기 위하여 도둑나방 유충 접종 밀도에 따른 양배추의 생육, 피해 및 수량을 조사하였다. 포장실험에서 파종 40일 양배추 유묘에 주당 0, 1, 4, 8, 16마리의 도둑나방 2령 유충들을 접종하고 30일 후 수량조사결과 처리 간 사이가 없었고, 양배추는 도둑나방 가해에 대하여 보상적 반응을 보였다. 반면, 유리온실 실험에서 정식 25일 양배추 유묘에 주당 0, 2, 5, 8, 12마리의 도둑나방 2령 유충들을 접종한 결과 15일 후 양배추의 평균생체중은 무처리에서 38.3g, 2마리 접종구에서 36.7g, 5마리 접종구에서 21.7g, 8마리 접종구에서 23.3g, 12마리 접종구에서 16.7g으로 도둑나방 유충의 접종수가 증가할수록 양배추의 생체중은 유의하게 줄어들었다. 도둑나방 유충 발생과 수량손실과의 관계를 구명하기 위하여 누적발생일수(cumulative insect days)와 양배추 유묘의 손실률(%)과의 관계를 회귀분석한 결과 비선형식인 logistic 모형에 잘 적용되었다. 이 식으로부터 수익한계인(gain threshold) 5%와 양배추 상품화율(91%)을 감안했을 때, 수량 감소율 14%에서 도둑나방 2령 유충의 경제적피해수준은 44 CID 이었다. 또한 요방제 밀도는 경제적 피해수준의 80%가 되는 35 CID로 추정되었다. 본 결과는 양배추 유묘시기에 도둑나방 유충을 방제하기 위한 경제적인 방제적기를 제공함으로써 본 해충의 효율적인 관리에 활용될 수 있을 것으로 판단된다.

• Animal cells and systems
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• 제14권1호
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• pp.31-36
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• 2010

The pinewood nematode Bursaphelenchus xylophilus causes pine wilt disease and is a serious economic concern for the forest industry of South Korea. To achieve effective control with limited resources, it is necessary to clarify the transmission routes and mechanisms of dispersal of this organism. Highly polymorphic and easy-to-use molecular markers can be used for investigating this aspect. In this study, we evaluated the usefulness of amplified fragment length polymorphisms (AFLPs) for investigating the genetic variations of B. xylophilus and related individuals from China, Japan, and South Korea. The AFLP patterns obtained in our study were similar to the microsatellite patterns reported in a previous study; our AFLP patterns indicated high genetic variability and cryptic genetic structure, but did not indicate any peculiar geographic structure. Moreover, the genetic distances between individuals suggested that the Korean population was affected to a greater extent by the Chinese population than the Japanese population. Further, the gene flow among the related species appeared to be limited; however, there may be also the possibility of genetic introgression among species. These results confirm the usefulness of AFLPs for understanding the epidemiology of pine wilt disease, thereby contributing to the effective control of this disease.

• Asian-Australasian Journal of Animal Sciences
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• 제12권4호
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• pp.642-650
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• 1999

Options available for utilization of animal wastes include sources of plant nutrients, feed ingredients for farm animals, substrate for methane generation, and substrate for microbial and insect protein synthesis. The wastes have the most economic value for use as animal feed. Performance of animals fed diets containing animal wastes is similar to that of animals fed conventional diets. Processing of animal wastes to be used as animal feed is necessary for destruction of pathogens, improvement of handling and storage characteristics, and maintenance or enhancement of palatability. Feeding of animal waste has not adversely affected the quality and taste of animal products. In the USA copper toxicity has been reported in sheep fed high-copper poultry litter, but this is not a serious problem with cattle. Potential pathogenic microorganisms in animal wastes are destroyed by processing such as heat treatment, ensiling and deep stacking. Incidents of botulism, caused by Clostridium botulinum, have been reported in cattle in some countries, and this problem was caused by the presence of poultry carcasses in litter. This problem has not occurred in the USA. With appropriate withdrawal, heavy metal, pesticide or medicinal drug accumulation in edible tissues of animals fed animal wastes is not a problem. Feeding of animal wastes is regulated by individual states in the USA. The practice is regulated in Canada, also. With good management, animal wastes can be used safely as animal feed.

• International Journal of Industrial Entomology and Biomaterials
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• 제19권2호
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• pp.265-268
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• 2009

Wild silkmoths can be utilised sustainably in the production of silk as an income for resource-poor rural communities. However, attack by parasitoids and predators affect the quality of cocoons and quantity of raw silk produced. A laboratory experiment was undertaken to quantify the effect of parasitoids' (dipteran and hymenopteran) and predators' (ants) exit and ingress holes, respectively, on silk production. The mean number of shells required to produce fifty grams of raw silk was highest with cocoons parasitised by a dipteran and lowest with unattacked cocoons (but with moths already emerged). Degumming loss was highest in parasitised and lowest in unttacked cocoons, but both were not different from cocoons predated by ants. Shell weight was highest in unattacked cocoons, followed by hymenopteran-parasitised and predated cocoons, with the dipteran parasitized ones being the least. Single cocoon weight was greater in hymenopteran-parasitised and predated cocoons than the dipteran-parasitised and unattacked cocoons. Shell ratio or raw silk, floss and yarn weights were higher in unattacked than parasitised and predated cocoons. The total loss in raw silk attributable to attack by parasitoids and predators ranged between 17.4~31.2%. The results offer baseline information for assessment of economic losses in wild silk farming due to parasitoids and predators in the field.

• 한국환경농학회지
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• 제28권3호
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• pp.301-309
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• 2009

The use of bioactive materials derived from microorganisms and plants has played a role in pest management in environmentally-friendly agriculture (EFA) system. In Korea, a number of agricultural agents for the control of insect pests have been registered officially as biopesticides and marketed widely. However, most of the biopesticides has a limitation in the resource availability of bioactive materials, which has been one of main problems related to the commercialization of agricultural agents. Plant materials and microbial metabolites are the best sources as starting components to commercialize natural-occurring agricultural agents for pest management. The lack of modernized system for the standardization and quality control of the starting materials, however, has also received as a main problem related to the commercialization of agricultural agents. Considered that EFA business has kept growing bigger and bigger with global economic status, the commercialization of agricultural agents is necessary to meet the required number of agricultural agents officially available in EFA. This study describes the status and future prospects of pest control agents in EFA. A number of main issues hindered in the commercialization of agricultural agents are discussed in order to present a promising approach to successful commercialization.

• Journal of the Korean Wood Science and Technology
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• 제42권2호
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• pp.149-156
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• 2014

Termites are wood pests that cause vast economic damage every year. They digest both cellulose and starch, but the enzymes for starch digestion have not been well characterized. We obtained complete amino acid sequence information on the KME1 ${\alpha}$-amylase from Reticulitermes speratus KMT1 through analysis of total mRNA sequences. The KME1 enzyme has two ${\alpha}$-amylase domains and is 68% identical to the ${\alpha}$-amylase from Blattellager manica, its closest relative in the GenBank database. Some unique features of its conserved region and its distant evolutionary relationship to other insect ${\alpha}$-amylases suggest that KME1 is a new type of ${\alpha}$-amylase.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.29-37
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• 2011

Commercially managed bees are available for pollination services and are used in large commercial fields, small gardens, or enclosures such as greenhouses and screen houses. This paper describes the current status and agricultural utilization of commercially managed bumblebees as well as bumblebee rearing techniques in Korea. We surveyed the use rate and number of bumblebees for the pollination of 10 major horticultural crops and fruit trees in Korea; in 2009, the use rates were approximately 7.9% and 2.8%, respectively. The use number of bumblebees as pollinators was more than 64,345 colonies, which included 51,400 colonies for 10 major horticultural crops and 12,945 colonies for 10 major fruit trees in 2009. The value of bumblebees as pollinators in 2009 in Korea was estimated at more than 5,100,000,000 won. We also describe feeding, rearing room conditions, colony initiation, mating and diapause breaks to establish year-round mass rearing techniques of the bumblebee Bombus ignitus.

• Journal of the Korean Wood Science and Technology
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• 제47권3호
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• pp.371-380
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• 2019

Termites are pests that cause serious economic and cultural damage by digesting wood cellulose. Termites are arthropods and have an epidermis surrounded by a chitin layer. To maintain a healthy epidermis, termites have chitinase (${\beta}$-1,4-poly-N-acetyl glucosamidinase, EC 3.2.1.14), an enzyme that hydrolyzes the ${\beta}$-1,4 bond of chitin. In this study, the amino acid sequence of the gene, which is presumed to be termite chitinolytic enzyme (NCBI accession no. KC477099), was obtained from a transcriptomic analysis of Reticulitermes speratus KMT001 in Bukhan Mountain, Korea. An NCBI protein BLAST search confirmed that the protein is a glycoside hydrolase family 18 (GH18). The highest homology value found was 47%, with a chitinase from Araneus ventricosus. Phylogenetic analysis indicated that the KC477099 protein has the same origins as those of arthropods but has a very low similarity with other arthropod chitinases, resulting in separation at an early stage of evolution. The KC477099 protein contains two conserved motifs, which encode the general enzymatic characteristics of the GH18 group. The amino acid sequences $Asp^{156}-Trp^{157}-Glu^{158}$, which play an important role in the enzymatic activity of the GH18 group, were also present. This study suggests that the termite KC477099 protein is a new type of chitinase, which is evolutionarily distant from other insect chitinases.

• 한국잠사곤충학회지
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• 제2권
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• pp.49-61
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• 1962

1. 경과습성 1) 수원지방에 있어서 뽕나무 순집이혹파리(Diplosis mori Yokoyama)는 5세대를 마친다. 제 1세대는 6월 하순경 제 2세대는 9월 하순경에 출현한다. 2) 성충은 오후 7~8시경에 출현하며 그 수명은 2~5일이며 ♀(자)가 ♂(웅)보다 약간 긴 듯하다. 3) 유충은 제 2~제 3 탁엽 AB 내면과 제 2~제 3 미개엽 이면에 기생한다. 따라서 생장점 부근에 피해를 준다. 4) 변태별 기간은 기상조건에 따라서 차가 심하며 특히 용은 한발시는 우화하지 못한다. 5) 유충부화시는 유백색이지만 등홍색으로 변하여 고숙하며 고숙유충은 15~20cm의 도요능력을 가지고 토중 15~30cm에 잠입화용한다. 그 크기는 0.3mm로부터 2.0mm 내외까지 발육한다. 6) 용기간은 하계는 7~8일 내외이며 조잡한 견에 쌓여있다. 7) 월동상태는 원칙적으로 용태월동을 하나 때에 따라서 유충월동도 있다. 2. 방제법 1) B.H.C. 분제 토양살포는 고숙유충이 입토 4~5일 후나 우화하기 전에 반당 2%액 400~600$\ell$(20~30두) 이상을 시용함이 효과적이라고 생각된다. 2) 매몰시험에 있어서는 심도 5cm 이상이면 성충우화를 방지할 수 있다고 인정된다.

• 한국산림과학회지
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• 제62권1호
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• pp.87-95
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• 1983

솔잎혹파리가 소나무 생장(生長)에 미치는 영향(影響)을 그 발생경과년수(發生經過年數)가 서로 다른 충청남도(忠清南道) 서천군(舒川郡), 보령군(保寧郡), 홍성군내(洪城郡內) 9개(個) 지역(地域)에서 조사(調査), 비교(比較)하였다. 이 조사(調査)는 피해허용한계(被害許容限界)를 알기 위한 첫 단계(段階)로 소나무피해(被害)의 개황(槪況)을 본 것으로 얻어진 결과(結果)는 다음과 같다. 1) 솔잎혹파리가 발생(發生)된 소나무의 피해(被害)는 4~5년간(年間) 지속(持續)되는데 2~3년차(年次)에 가장 심(甚)하였다. 2) 솔잎혹파리의 충영형성(蟲廮形成)은 소나무의 직경생장(直径生長)과 수고생장(樹高生長)의 저해(沮害)를 동시(同時)에 일으킨다. 3) 소나무의 직경생장(直径生長)과 수고생장간(樹高生長間)에는 밀접(密接)한 상관관계(相關關係)가 있었으며 솔잎혹파리 피해(被害)에도 불구하고 이 관계(關係)는 교란되지 않았다. 따라서, 주신초(主新梢)의 생장저해(生長沮害) 상황(狀況)을 가지고 직경생장(直径生長)의 저해정도(沮害程度)를 추정(推定)할 수 있을 것이다. 4) 솔잎혹파리의 충영율(蟲廮率)은 북향(北向)에서 월등(越等)하게 높아 소나무의 고사(枯死)도 북향(北向)에서 높았다. 5) 솔잎혹파리로 인(因)한 13년생(年生) 소나무의 재적생장손실(材積生長損失)은 수확표와 비교(比較)할 때 75~85% 이상(以上)이 되고 이 피해(被害)는 정상적(正常的)인 생장(生長)을 되찾아도 복구(復舊)되기 어려울 것 같다.