• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.176-177
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.72-73
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.74-75
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• 2005

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.268-273
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• 2003

To identify the variation of the RAPD patterns between two Atractylodes species, 52 kinds of random primers were applied to each eight of A japonica and A. macrocephala genomic DNA. Ten primers of 52 primers could be used to discriminate between the species and 18 polymorphisms among 67 scored DNA fragments (18 fragments are specific for A. japonica and A. macrocephala) were generated using these primers, 26.9% of which were polymorphic. RAPD data from the 10 primers was used for cluster analysis. The cluster analysis of RAPD markers showed that the two groups are genetically distinct. On the other hand, to identify the variation of the AFLP patterns and select the species specific AFLP markers, eight combinations of EcoRI/MseI primers were applied to the bulked A. japonica and A. macrocephala genomic DNA. Consequently, three combinations of EcoRI/MseI primers (EcoRI /Mse I ; AAC/CTA, AAC/CAA, AAG/CTA) used in this study revealed 176 reliable AFLP markers, 42.0% of which were polymorphic. 74 polymorphisms out of 176 scored DNA fragments were enough to clearly discriminate between two Atractylodes species.

• Korean Journal of Medicinal Crop Science
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• v.22 no.4
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• pp.270-275
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• 2014

This study was conducted to select and develop effect of eco-friendly organic materials for the eco-friendly prevention of Anthracnose occurred in the ginseng. Anthracnose on ginseng is occurred by Colletotrichum gloeosporioides and the crop damage is severe especially from July to August after rainy season. The test results showed that control effect by test products materials on the three years ginseng and four years ginseng field was lower in eco-friendly organic materials than that of chemical pesticide. However, the control effect of bordeaux mixture was higher with 71.3% and 73.8% levels than those of mineral matter, microbial agent, and developed plants extract mixtures (Eugenol, Curcumin, Wood vinegar, etc). On the other hand, three types of developed plants extract mixtures (3 types) showed control effect in a range of from 58.1% to 63.6% against Anthracnose which was higher as compared with plant extract alone and sodium silicate regardless of ages of ginseng. The results of this study would attribute in verifying the control effect of eco-friendly materials against Anthracnose for ginseng through investigating antimicrobial compounds contained in the plants body. Also, it would be used as control method against Anthracnose occurred in ginseng by judging the right control time through monitoring occurrence of disease.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.178-181
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• 2011

The test was carried out at a test field at the Wanju Eco-Farming Complex from 2009 to 2010 to figure out the proper application of fertilizers when growing rice at the Eco-Farming Complex. The result showed that when compared to the basal application of compost as fertilizer, applying supplementary compost after natural re-seeding of chinese milk vetch (CMV) helped balance soil nutrition and maintained rice yields.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.3
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• pp.164-173
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• 2017

Alternative cropping systems would be one of climate change adaptation options. Suitable areas for a crop could be identified using a climate suitability model. The EcoCrop model has been used to assess climate suitability of crops using monthly climate surfaces, e.g., the digital climate map at high spatial resolution. Still, a high-performance computing approach would be needed for assessment of climate suitability to take into account a complex terrain in Korea, which requires considerably large climate data sets. The objectives of this study were to implement a script for R, which is an open source statistics analysis platform, in order to use the EcoCrop model under a parallel computing environment and to assess climate suitability of maize using digital climate maps at high spatial resolution, e.g., 1 km. The total running time reduced as the number of CPU (Central Processing Unit) core increased although the speedup with increasing number of CPU cores was not linear. For example, the wall clock time for assessing climate suitability index at 1 km spatial resolution reduced by 90% with 16 CPU cores. However, it took about 1.5 time to compute climate suitability index compared with a theoretical time for the given number of CPU. Implementation of climate suitability assessment system based on the MPI (Message Passing Interface) would allow support for the digital climate map at ultra-high spatial resolution, e.g., 30m, which would help site-specific design of cropping system for climate change adaptation.

• Korean Journal of Medicinal Crop Science
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• v.19 no.3
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• pp.198-204
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• 2011

This study was performed to enhance antifungal activity of anthracnose in chili pepper by nanopaticles of thiamine di-lauryl sulfate (TDS) through high pressure homogenization process. Yield of TDS was 79.14% by reaction of thiamine hydrochloride and sodium lauryl sulfate. TDS nanopaticle solution was manufactured through high pressure homogenization process. The turbidity of nanoparticles solution was increased with increasing the concentration of TDS, and nanoparticles solution of 100 ppm was showed the highest turbidity with absorbance of 3.212. The size of nanoparticles solution was measured as average 258.6 nm by DLS. Nanoparticles solution of 100 ppm showed growth inhibition activity with higher than about 80% compared to the control group against Colletotrichum gloeosporioides. Finally, nanoparticles solution was increased effectively the penetration of the TDS nanopaticles on attached cell membrane of hyphae and started to destruct the cells under microscope observation. Consequently, we suggested that the TDS nanoparticle solution by high pressure homogenization process might be suitable biochemical pesticides for improving the antifungal activities against anthracnose in pepper.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.1
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• pp.53-56
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• 2001

Barley Yellow Dwarf Virus (BYDV), an aphid-borne luteovirus, is a major plant pathogenic disease causing a huge economic loss in the grain production of a wide range of Gramineae species throughout the world. It has been recently reported that BYDV also occurred frequently in wheat field of Korea. Here, we performed to develop the detection and classification methods of BYDV strains that were accomplished by reverse transcription-polymerase chain reaction (RT-PCR). Since there are high variations among BYDV strains, three pairs of primers were designed to detect BYDV strains such as PAV (Vic-PAV and CN-PAV) and MAV (primer A) simultaneously, specifically Vic-PAV(primer B), and MAV (primer C) based on the genomic RNA sequences of BYDV strains previously published. The validity of the primers was confirmed using several BYDV strains obtained from CIMMYT. Though three BYDV strains were able to be detected using primer A, PCR products were not distinguished between two PAV strains. It was possible to separate them with a restriction enzyme, EcoRI, whose restriction site was present in the amplified DNA fragment from Vic-PAV, but not from CN-PAV.

• Research in Plant Disease
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• v.18 no.4
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• pp.399-401
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• 2012

In order to select the optimum rice cultivars for the use in processing of eco-friendly-grown germinated brown rice (GBR), disease incidences and yields in rice cultivars bred in National Institute of Crop Science were investigated in eco-friendly paddy fields (Gokseong, Jeonnam Province, Korea) during the years 2009-2011. The incidences of rice sheath blight and blast on the cultivar Samgwang were higher than those on one reference cultivar Chucheong, but much lower than those on the other reference cultivar Gosihikkali. The cultivars Keunnun and Haiami selected as special rices showed disease incidences and yields similar to the reference rice cultivars Chucheong and Gosihikkali. These results indicated that the cultivars Samkwang, Keunnun, and Haiami can be selected as rice cultivars for the use of in processing of eco-friendly grown GBR because their disease incidences and yields in Gokseong may be similar to the reference cultivars.