• Animal Bioscience
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• 제34권6호
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• pp.1078-1087
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• 2021

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.42-42
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• 2022

• Structural Engineering and Mechanics
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• 제69권6호
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• pp.627-635
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• 2019

In order to study the axial compression performance of sand-lightweight concrete-filled steel tube (SLCFST) stub columns, three circular SLCFST (C-SLCFST) stub column specimens and three SLCFST square (S-SLCFST) stub column specimens were fabricated and static monotonic axial compression performance testing was carried out, using the volume ratio between river sand and ceramic sand in sand-lightweight concrete (SLC) as a varying parameter. The stress process and failure mode of the specimens were observed, stress-strain curves were obtained and analysed for the specimens, and the ultimate bearing capacity of SLCFST stub column specimens was calculated based on unified strength theory, limit equilibrium theory and superposition theory. The results show that the outer steel tubes of SLCFST stub columns buckled outward, core SLC was crushed, and the damage to the upper parts of the S-SLCFST stub columns was more serious than for C-SLCFST stub columns. Three stages can be identified in the stress-strain curves of SLCFST stub columns: an elastic stage, an elastic-plastic stage and a plastic stage. It is suggested that AIJ-1997, CECS 159:2004 or AIJ-1997, based on superposition theory, can be used to design the ultimate bearing capacity under axial compression for C-SLCFST and S-SLCFST stub columns; for varying replacement ratios of natural river sand, the calculated stress-strain curves for SLCFST stub columns under axial compression show good fitting to the test measure curves.

• Smart Structures and Systems
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• 제23권6호
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• pp.627-639
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• 2019

Passive control may not provide enough damping for a stay cable since the control devices are often restricted to a low location level. In order to enhance control performance of conventional passive dampers, a new type of damper integrated with a rotary electromagnetic damper providing variable damping force and a flywheel serving as an inertial mass, called the rotary electromagnetic inertial mass damper (REIMD), is presented for suppressing the cable vibrations in this paper. The mechanical model of the REIMD is theoretically derived according to generation mechanisms of the damping force and the inertial force, and further validated by performance tests. General dynamic characteristics of an idealized taut cable with a REIMD installed close to the cable end are theoretically investigated, and parametric analysis are then conducted to investigate the effects of inertial mass and damping coefficient on vibration control performance. Finally, vibration control tests on a scaled cable model with a REIMD are performed to further verify mitigation performance through the first two modal additional damping ratios of the cable. Both the theoretical and experimental results show that control performance of the cable with the REIMD are much better than those of conventional passive viscous dampers, which mainly attributes to the increment of the damper displacement due to the inertial mass induced negative stiffness effects of the REIMD. Moreover, it is concluded that both inertial mass and damping coefficient of an optimum REIMD will decrease with the increase of the mode order of the cable, and oversize inertial mass may lead to negative effect on the control performance.

• 한국주거학회논문집
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• 제13권1호
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• pp.29-36
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• 2002

This study was performed to investigate the awareness of Namak Eco-city, a willingness to move to an ecological city and their related variables. Data was collected through a questionnaire survey given to 491 residents who live in Mokpo, Kwangju and Muan in Chollanam Province. The awareness of Namak Eco-city was medium level and half of the respondents were not aware of that. Multiple regression analysis was conducted to estimate the effects of the variables on the awareness of Namak Eco-city. The most significant variable was the awareness of taco-city. Thirty eight percent of the respondents were willing to move to Namak Eco-City. Two variables were related with willingness to move to the city. Those who were aware of an Eco-city and had more commuting-oriented housing value had more willingness to move to the city.

• Smart Structures and Systems
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• 제24권1호
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• pp.83-94
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• 2019

Passive negative stiffness dampers (NSDs) that possess superior energy dissipation abilities, have been proved to be more efficient than commonly adopted passive viscous dampers in controlling stay cable vibrations. Recently, inertial mass dampers (IMDs) have attracted extensive attentions since their properties are similar to NSDs. It has been theoretically predicted that superior supplemental damping can be generated for a taut cable with an IMD. This paper aims to theoretically investigate the impact of the cable sag on the efficiency of an IMD in controlling stay cable vibrations, and experimentally validate superior vibration mitigation performance of the IMD. Both the numerical and asymptotic solutions were obtained for an inclined sag cable with an IMD installed close to the cable end. Based on the asymptotic solution, the cable attainable maximum modal damping ratio and the corresponding optimal damping coefficient of the IMD were derived for a given inertial mass. An electromagnetic IMD (EIMD) with adjustable inertial mass was developed to investigate the effects of inertial mass and cable sag on the vibration mitigation performance of two model cables with different sags through series of first modal free vibration tests. The results show that the sag generally reduces the attainable first modal damping ratio of the cable with a passive viscous damper, while tends to increase the cable maximum attainable modal damping ratio provided by the IMD. The cable sag also decreases the optimum damping coefficient of the IMD when the inertial mass is less than its optimal value. The theoretically predicted first modal damping ratio of the cable with an IMD, taking into account the sag generally, agrees well with that identified from experimental results, while it will be significantly overestimated with a taut-cable model, especially for the cable with large sag.

• 한국의류산업학회지
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• 제15권5호
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• pp.722-732
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• 2013

The purposes of this study were to investigate the needs for eco t-shirts of the new elderly consumers and to suggest strategies for eco t-shirts product development and marketing. Quantitative research was employed for research method. The data was collected from men and women in their age of 50-65, who lived in Busan, Ulsan, and Kyungnam province, during October, 2011. Respondents were selected by convenience sampling. A total of 307 responses among 350 were analyzed. Factor analysis, frequency analysis, t-test, chi-square, and regression were used for analysis. SPSS 20.0 was used for data analysis. Characteristics of the new elderly (lifestyle and environmental concern), consumption status of eco t-shirts (possession status and reasons of purchase/non-purchase), purchase intention for eco tshirts, consumer needs for t-shirts (usage situation, benefits sought, and dissatisfaction with t-shirts), preference for eco t-shirts (color, pattern, material, and shape), and demographics were surveyed. Based on the results, the vision of eco tshirts market for the new elderly consumers was positively prospected. In conclusion, specific strategies for eco t-shirts products development and marketing were suggested. Product development focused on practicality and material, design development reflecting consumers' preference, multipurpose product development, building up the product recognition, and moderate price strategy were suggested.

• Journal of Ginseng Research
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• 제43권4호
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• pp.600-605
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• 2019

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

• 한국조경학회지
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• 제32권3호
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• pp.32-42
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• 2004

This study aims at establishing an ecological planning for road construction. which is considered to be a main cause of damage to the natural environment in Korea. This study focuses on the Pyungtak-Eumsung Highway development project. It considers the ecological factors and status of the site and its surrounding area. The study site is a four-way highway with a width of 23.4m and a length of 5.7km that spans from Hyungok-ri, Anseong City, Kyeonggi Province to Jukhyun-ri, Jincheon-gun, Chungbuk Province. The objective of the plan is "the establishment of an eco-road in harmony with nature." The plan is divided into five detailed goals: 1) restoration of river morphology and ecosystem through ecological planning; 2) establishment of wet biotopes; 3) construction of ecological corridors; 4) restoration of damaged forest ecosystems; and 5) ecological restoration of the roadside slopes that are linked with the surrounding forest. A master plan has been developed based on the detailed goals. The master plan involves: 1) establishment of a natural river, wet biotopes, and ecological corridors that facilitate the movement of amphibians, wild fowls, mammalians and fish; 2) development of a planting plan for the visitor center, the tunnel entrance, and soundproof banks; and 3) the presentation of a planting model for restoring roadside slopes that are connected to the surrounding forest. The eco-road plan needs to entail ecological conservation and restoration plans. In addition, a monitoring plan for ecological corridors and habitats should be included in the comprehensive plans, along with the continuous development of environmentally friendly technologies.

• 한국조경학회지
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• 제34권4호
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• pp.37-47
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• 2006

The city of Siheung in the Kyunggi-do Province has various natural resources such as the ocean, mountains, wide farmland, various types of wetlands, streams and rivers. In addition, the city has a big greenbelt, which consists of two-thirds of the area, where development has been regulated. Since the city has a relatively well-preserved natural environment, it offers a great potential for regional development. The purpose of this study is to create an eco-tourism resource plan for the Mulwang reservoir, which offers many opportunities for ecotourism in the city of Siheung. This study includes a literature review for planning elements and suggests a comprehensive plan that includes conservation, eco-restoration, route program and practice program for ecotourism in and around the site. The plan also includes eco-farming, a visitor center, an ecovillage, the chance to experience livestock farming, opportunities to learn about and experience the forest, tracking, eco-learning, an environmental interpretation facility, fishing and aquatic-oriented leisure activities. This study's process and results show possibilities that can be applied to other areas where eco-tourism using natural resources is used for regional development.