• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.386-392
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• 2015

A novel recombinant bacmid, bEasyBm, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. In bEasyBm, attR recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. Moreover, the extracellular RNase gene from Bacillus amyloliquefaciens, barnase, was expressed under the control of the Cotesia plutellae bracovirus early promoter to negatively select against the nonrecombinant background. The bEasyBm bacmid could only replicate in host insect cells when the barnase gene was replaced with the gene of interest by in vitro transposition. When bEasyBm was transposed with pDualBac-EGFP, the resulting recombinant virus, EasyBm-EGFP, showed high levels of EGFP expression efficiency compared with that of non-purified recombinant virus BmGOZA-EGFP, which was constructed using the bBmGOZA system. In addition, nonrecombinant backgrounds were not detected in unpurified EasyBm-EGFP stocks. Based on these results, a high-throughput system for the generation of multiple recombinant viruses at a time was established.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.6 no.2
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• pp.113-118
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• 2007

KPCS internal antenna in this paper, is designed and fabricated using the theory based on the folded monopole antenna. I propose folded monopole internal antenna which has high gain low profile structure with easy installation into the handset of mobile phone. Design frequency is 1.8 GHz that is a center frequency of Korea PCS frequency band, and the impedance variances due to change of antenna structure as a length and width are simulated. Measurements show that input VSWR range under 2:1 is 1.72 - 1.88 GHz, and radiated H-plane pattern is omnidirectional under passive state. Active sensitivities and output power of phone applied antenna are $-105\;{\sim}\;-108\;dBm$ and $22.5\;{\sim}\;23.6\;dBm$ max., respectively.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.16 no.8 s.99
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• pp.797-802
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• 2005

In this paper, we design a UHF band RFID tag antenna which is conjugate matched to an impedance of a chip and also mountable on conductive materials. The proposed tag antenna is very compact($50{\times}30{\times}4mm$) with a modified PIFA shape. The proposed tag antenna has an advantage of easy matching to various chip input impedances. The performance of the antenna is evaluated by monitoring RCS in the reader direction. The RCS of the designed tag is $-10.2\;dBm^2$ when the chip is shorted and is $-21\;dBm^2$ when the chip impedance is a complex conjugate of the antenna impedance.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.12 s.115
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• pp.1240-1248
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• 2006

This paper presents an UHF band(911 MHz) RFID tag antenna which has near-isotropic radiation pattern and easy conjugate impedance matching characteristics to any commercial chips of usual practice through the application of an inductively-coupled feeding. The proposed antenna of compact size $40{\times}46mm\;(0.12{\times}0.14{\lambda})$ has, at normal incidence, the maximum RCS of $-18.5dBm^2$ and the 3 dB RCS bandwidth of 9 MHz(1 %) in case of short chip load. It has the maximum and minimum RCS' of $-16.9dBm^2\;and\;-21.4dBm^2$ depending on the incident angles. The difference of about 4.5 dB is relatively small compared with that (about 70 dB) of a pure dipole antenna. The designed antenna has been fabricated and its RCS' have been measured varying the angles of incidence. The measured RCS' have been found to have good agreement with the simulated ones.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.315-319
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• 2003

A novel method is developed to yield virus titers in 10 h, is easy to .perform using 96-well plates, and applicable to both any Autographa californica nucleopolyhyderovirus (AcNPV) and Bombyx mori nucleopolyhedrovirus (BmNPV)-based recombinant baculovirus. This assay uses an antibody to a DNA-binding protein to detect the infected cells via immune-staining. The titer is determined by counting foci produced due to infection of virus under a fluorescent microscopy. The required incubation period was shortened considerably because infected cells expressed viral antigens at the post infection time of 4 h. Therefore, 10 hours were enough to estimate the virus titer including virus infection time, insect cell culture, and estimation of virus titer.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.5 no.2
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• pp.61-67
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• 2012

In this paper, In this paper, we consider a dynamic range in the frequency converter to obtain a high conversion gain and linearity while operating area proposed to broaden the design. Super-heterodyne RF Front-End style was applied to the active mixer stage, GaAs devices were used. Circuit design easy and simple forms benefit circuit is constructed in the drain mixer, passive mixer with the operating area were compared and analyzed. The simulation results of the conversion gain of 2.4dB and 0.2dBm about a gain-compression point, and showed the dynamic range of 71.9dB, when compared with passive mixers, dynamic range of approximately 6dB improvement was identified. Measurements of an approximately 2dB conversion gain and-1.0dBm of the gain-compression point, and confirmed that the active area of 71.1dB. When compared with passive mixers, dynamic range of is reduced by approximately 8dB has been improved.

• Journal of the Institute of Convergence Signal Processing
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• v.13 no.1
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• pp.39-43
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• 2012

60GHz band RF transceiver was made with the NRD waveguide structure for the point- to-point communication. A dielectric line that of comprising NRD waveguide was the milling process was not easy because a material gets soft, and also compression and expansion according to a temperature were serious, so this line was not suitable for the device in which the resonance characteristic was important. In addition, the thing for comprising amplification module was difficult in the NRD waveguide structure. In this paper, a way in which to overcome mentioned in upper part, the transceiver was made by below technology. Components in which the resonance characteristic was not important were made with the NRD waveguide hybrid IC, and components in which the resonance characteristic was important were made with waveguide. An amplifier packaged and modularizing the bare chip, it equipped at the NRD waveguide within. Manufactured transceiver communicated with FDD method, and it had 10dBm output power, and -60dBm minimum receive sensitivity.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.42 no.4
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• pp.889-891
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• 2017

Visible light communication technology, which is a communication using LED lighting, is defined by IEEE 802.15.7 WG and active research is under way. Visible light communication is advantageous not only to avoid interference with existing RF communication but also to provide location based service through accurate positioning by utilizing LOS (Line of Sight) characteristic. Therefore, it is very easy and efficient to locate and track the user's location. In this paper, we implemented a visible light communication transceiver using USB interface for easy application to portable devices. It supports the mobility of mobile devices through internet protocol and showed BER performance of less than $10^{-3}dBm$ at over 1m, which is the height of lighting and smart device during walking.

• Culinary science and hospitality research
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• v.21 no.6
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• pp.242-254
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• 2015

Barley is a main food source, along with rice, in our dietary life that is easy to buy and process. It is required to develop a beverage base and barley syrup for desserts in order to raise utilization of barley beyond its present use in Sikhye (sweet fermented rice drink) and Jocheong (grain syrup) production. In pursuit of the goal to increase the usability of barley as an ingredient of processed food, this study examined the optimal preparation conditions of barley mash for barley syrup. In addition, the study prepared a barley syrup using saccharified barley liquid, analyzed quality characteristics, and conducted a sensory evaluation. Saccharified barley liquid(barley mash) was prepared with ratios of adding malt of unhulled barley germinated as 0 g(CON), 10 g(BM10), 20 g(BM20), and 30 g(BM30). The results of measuring pH under the varied conditions of saccharification temperatures from $50^{\circ}C$, $60^{\circ}C$, and $70^{\circ}C$, as well as time from 1 to 8 hours, treveal that the optimum condition for malt saccharification is $60^{\circ}C$ for 5 hours. By adding oligosaccharides and lemon juice to the saccharified barley liquid at the different ratios of added malt, this study measured quality characteristics(color value, viscosity, pH, sweetness) of barley syrup warmed up for 1 hour. As the result of measuring color in the barley syrup prepared by saccharified barley liquid, higher levels of added malt saw L-value decreased while a-value and b-value both increased. BMs30 showed the highest viscosity of $1,202.67{\pm}3.06$. As for pH, BMs30 was the highest at pH $3.57{\pm}0.02$. The result of the sensory evaluation of barley syrup showed the superior sensory characteristics of BMs20 in terms of color, flavor, sweetness, viscosity and overall quality.

• Journal of electromagnetic engineering and science
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• v.5 no.1
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• pp.8-13
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• 2005

The InGaP/GaAs hetero-junction bipolar transistor(HBT) monolithic voltage-controlled dielectric resonator oscillator(VCDRO) is first demonstrated for a Ku-band low noise block down-converter(LNB) system. The on-chip voltage control oscillator core employing base-collector(B-C) junction diodes is proposed for simpler frequency tuning and easy fabrication instead of the general off-chip varactor diodes. The fabricated VCDRO achieves a high output power of 6.45 to 5.31 dBm and a wide frequency tuning range of ]65 MHz( 1.53 $\%$) with a low phase noise of below -95dBc/Hz at 100 kHz offset and -115 dBc/Hz at ] MHz offset. A]so, the InGaP/GaAs HBT monolithic DRO with the same topology as the proposed VCDRO is fabricated to verify that the intrinsic low l/f noise of the HBT and the high Q of the DR contribute to the low phase noise performance. The fabricated DRO exhibits an output power of 1.33 dBm, and an extremely low phase noise of -109 dBc/Hz at 100 kHz and -131 dBc/Hz at ] MHz offset from the 10.75 GHz oscillation frequency.