• 한국전자통신학회논문지
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• 제18권2호
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• pp.373-378
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• 2023

희귀질환은 증상이 전형적이지 않고 진단정보가 부족하여 전문의들조차 증상을 기반으로 질환을 의심하거나 질환명을 떠올리는 데에 어려움을 겪는다. 따라서 증상이 시작한 시점에서부터 정확한 진단을 받기까지 많은 시간 및 비용이 발생하며, 이는 환자의 신체적, 정신적, 경제적 부담을 심각하게 초래한다. 환자의 증상정보를 통해 의심되는 희귀질환을 제시하여 의사의 진단에 활용할 수 있도록, 본 논문에서는 웹 크롤링 및 텍스트마이닝을 활용한 희귀질환 조기 발견 보조시스템을 제안하고 이를 구현한다.

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.329-334
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• 2016

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

• 대한수의학회지
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• 제43권2호
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• pp.239-246
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• 2003

Polymerase chain reaction (PCR) was evaluated for the early detection of Aujeszky's disease virus (ADV) DNA from virus-infected cell cultures. For the purposes, the Korean ADV NYJ1-87 was propagated in swine kidney (SK) cells and subjected to the amplification of DNA (217 bp) by PCR using sense and antisense primers specific to gp50 gene of the ADV. In detection of cell-associated viral DNA, reliable PCR conditions were determined as 30 cycles of reaction consisting 1 minute each of denaturation at $94^{\circ}C$, annealing at $55^{\circ}C$ and polymerization at $72^{\circ}C$. The PCR encountered best results with reagent mixtures of $50{\mu}l$ containing $200{\mu}M$ dNTPs, $0.2{\mu}M$ each sense and antisense primers, 1 mM $MgCl_2$ and 10% (v/v) template DNA in the final concentrations. ADV-specific DNAs were detected as early as 6, 6, and 9 hours post-infection, respectively, from lysates of the SK cells infected with ADV of $10^3$, $10^2$ and $10^1\;TCID_{50}/ml$ by this condition. In culture supernatant, the DNAs were detected from ADV of as low infectivity as $10^ {-3}\;TCID_{50}/ml$ by the reduced reagent concentrations and 30 cycles of 1 minute each of denaturation at $94^{\circ}C$ and annealing at $55^{\circ}C$, and 2 minutes of polymerization at $72^{\circ}C$. The lowest amount of detectable ADV DNA was 1 fg. In conclusion, the PCR condition established in the present study was recognized as a feasible alternative to time-consuming procedures in isolation and characterization of the virus.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.100-108
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• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• Clinical and Experimental Pediatrics
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• 제57권8호
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• pp.357-362
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• 2014

Purpose: The incidence of Kawasaki disease (KD) is rare in young infants (less than 3 months of age), who present with only a few symptoms that fulfill the clinical diagnostic criteria. The diagnosis for KD can therefore be delayed, leading to a high risk of cardiac complications. We examined the clinical characteristics and measured the serum levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) levels of these patients for assessing its value in the early detection of KD. Methods: We retrospectively reviewed the data of young infants diagnosed with KD from 2004 to 2012. The control group included 20 hospitalized febrile patients. Laboratory data, including NT-proBNP were obtained for each patient in both groups. Results: Incomplete KD was observed in 21/24 patients (87.5%). The mean fever duration on admission was $1.36{\pm}1.0$ days in the KD group. Common symptoms included erythema at the site of Bacille Calmette-Guerin inoculation (70.8%), skin rash (50.0%), changes of oropharyngeal mucosa (29.1%), and cervical lymphadenopathy (20.8%). The mean number of major diagnostic criteria fulfilled was $2.8{\pm}1.4$. Five KD patients (20.8%) had only one symptom matching these criteria. The incidence of coronary artery complications was 12.5%. The mean serum NT-proBNP level in the acute phase, in the KD and control groups, were $4,159{\pm}3,714pg/mL$ and $957{\pm}902pg/mL$, respectively, which decreased significantly in the convalescent phase. Conclusion: Incomplete KD was observed in 87.5% patients. Serum NT- proBNP might be a valuable biomarker for the early detection of KD in febrile infants aged <3 months.

• 한국컴퓨터정보학회논문지
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• 제28권12호
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• pp.67-77
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• 2023

본 연구는 스마트팜 환경에서 진행된 혁신적인 연구로, 딥러닝을 기반으로 한 질병 및 해충 탐지 모델을 개발하고, 이를 지능형 사물인터넷(IoT) 플랫폼에 적용하여 디지털 농업 환경 구현의 새로운 가능성을 탐색하였다. 연구의 핵심은 Pseudo-Labeling, RegNet, EfficientNet 등 최신 ImageNet 모델과 전처리 방식을 통합하여, 복잡한 농업 환경에서 다양한 질병과 해충을 높은 정확도로 탐지하는 것이었다. 이를 위해 앙상블 학습 기법을 적용하여 모델의 정확도와 안정성을 극대화했으며, 평균 정밀도(mAP), 정밀도, 재현율, 정확도, 박스 손실 등의 다양한 성능 지표를 통해 모델을 평가하였다. 또한, SHAP 프레임워크를 활용하여 모델의 예측 기준에 대한 깊은 이해를 도모하였고, 이를 통해 모델의 결정 과정을 보다 투명하게 만들었다. 이러한 분석은 모델이 어떻게 다양한 변수들을 고려하여 질병 및 해충을 탐지하는지에 대한 중요한 통찰력을 제공하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.6141-6147
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• 2015

Background: Cancer is a leading cause of death worldwide. A large proportion of cancer deaths are preventable through early detection but there are a range of social, emotional, cultural and financial dimensions that hinder the effectiveness of cancer prevention and treatment efforts. Cancer stigma is one such barrier and is increasingly recognized as an important factor influencing health awareness and promotion, and hence, disease prevention and control. The impact and extent of stigma on the cancer early detection and care continuum is poorly understood in India. Objectives: To evaluate cancer awareness and stigma from multiple stakeholder perspectives in North India, including men and women from the general population, health care professionals and educators, and cancer survivors. Materials and Methods: A qualitative study was conducted with in-depth interviews (IDIs) and focus group discussions (FGDs) among 39 individuals over a period of 3 months in 2014. Three groups of participants were chosen purposively - 1) men and women who attended cancer screening camps held by the Indian Cancer Society, Delhi; 2) health care providers and 3) cancer survivors. Results: Most participants were unaware of what cancers are in general, their causes and ways of prevention. Attitudes of families towards cancer patients were observed to be positive and caring. Nevertheless, stigma and its impact emerged as a cross cutting theme across all groups. Cost of treatment, lack of awarenes and beliefs in alternate medicines were identified as some of the major barriers to seeking care. Conclusions: This study suggests a need for spreading awareness, knowledge about cancers and assessing associated impact among the people. Also Future research is recommended to help eradicate stigma from the society and reduce cancer-related stigma in the Indian context.

• 생물정신의학
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• 제20권2호
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• pp.31-39
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• 2013

The geriatric patients with chronic physical diseases are frequently associated with the continuous clusters of depression including nonpathological sadness, subsyndromal depression, minor depressive disorder, and major depressive disorder. Because of the complex and reciprocal relationships among depression, elderly, and chronic physical diseases, screening approaches with specific nosological methods should be needed in the realm of early detection of depression. Cognitive decline is frequently manifested in geriatric depression with medical or neurological diseases. Also, somatic symptoms of depression or emotional symptoms of physical diseases can play a role as a hampering factor in the early detection of depression. Furthermore, after-care has been regarded as an essential factor of depression screening in the geriatric patients with chronic physical diseases. We reviewed the most popular examples of integrated medicine for depression in primary care. Thus, we propose a general hospital-based model for early detection of depression which includes favorable response loop between screening and therapeutic intervention. Our model can be a basis for evidence-based detection and after-care for depression in the geriatric patients with chronic medical diseases.

• 보건교육건강증진학회지
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• 제7권1호
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• pp.64-71
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• 1990

The purpose of this study is to identify the main factors influencing breast cancer self-examination, a preventive health behavior, thereby increasing self-examination compliance for early detection of the disease. The data on which this study was based were collected from a survey of 601 ladies, aged 20∼59 years and residing in Seoul, employing such mehtods as X²-test, ANOVA, t-test, F-test, Person's Correlation Coefficient and Stepwise Multiple Regression. The resulting conclusions are as follows; 1. Discrepancies in self-examination compliance rate are found in accordance with the differences of general characters of the surveyed persons. For instance, those who are well educated and better off are better compliers than those who are not (p<0.001), and those around whom breast cancer patients are better ones than who are not (p<0.01). 2. Self-examination compliers have higher health belief than non-compliers. Compliers have more knowledge in health and have higher susceptibility, barriers and health concern (p<0.001), and higher benefits (p<0.01), and higher seriousness (p<0.05) than non-compliers. 3. Whereas those who have loftier health belief show higher compliance rate (p<0.001), seriousness turned out to have no correlationship with self-examination compliance. 4. Stepwise Multiple Regression portray that following factors influence self-examination compliance in arder named. (1) barriers (2) susceptibility, (3) health concern, (4) age, (5) benefits, (6) education level. Even so, it turned out that these factors alone can explain only 20％ of self-examination compliance. Therefore study for the other factors ought to be continued. I submit following suggestions ending this study. 1. Since breast cancer self-examination is an essential health behavior needed for early detection of the disease, efficient and proper health education program eyed for regular and periodic self-examination is required to be developed, thus reducing the deaths and pains caused by the disease. 2. Proper policies of the government for the prevention of breast cancer is strongly urged to be formed in concrete manner. 3. Continuous study of the other factors affecting self-examination compliance must be carried on.

• Journal of Yeungnam Medical Science
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• 제37권4호
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• pp.332-336
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• 2020

Homozygous mutations in NUDT15 R139C are known as the major factor associated with thiopurine-induced early leukopenia, particularly in Asian patients. Therefore, NUDT15 genotyping is currently recommended before thiopurine treatment to identify patients who are NUDT15 poor metabolizers and consider the use of an alternative immunomodulatory therapy. We report a case of a 12-year-old Korean girl with Crohn's disease (CD), in whom thiopurine-induced leukopenia was prevented by initiation of azathioprine (AZA) therapy at a low dose (0.5 mg/kg/day) and early detection of significant hair loss and white blood cell (WBC) count decrease at 17 days from the start of AZA treatment. The WBC count dropped from 8,970/μL to 3,370/μL in 2 weeks, and AZA treatment was stopped because of concerns of potential leukopenia in the near future. Her WBC count recovered to 5,120/μL after 3 weeks. Gene analysis later revealed that she had a homozygous mutation in NUDT15 R139C, resulting in a poor metabolizing activity of NUDT15. In situations when NUDT15 genotyping is unavailable, initiation of AZA therapy at 0.5 mg/kg/day with close observation of hair loss and WBC counts within 2 weeks may be an alternative way to prevent thiopurine-induced early leukopenia in Asian children with CD.