Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis

  • Goo, Youn-Kyoung (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Shin, Won-Sik (Department of Obstetrics and Gynecology, Shinsegae Women's Hospital) ;
  • Yang, Hye-Won (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Joo, So-Young (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Song, Su-Min (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Ryu, Jae-Sook (Department of Environmental Biology & Medical Parasitology, Hanyang University College of Medicine) ;
  • Kong, Hyun-Hee (Department of Parasitology, Dong-A University College of Medicine) ;
  • Lee, Won-Ki (Center of Biostatistics, Kyungpook National University School of Medicine) ;
  • Chung, Dong-Il (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Hong, Yeonchul (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine)
  • Received : 2016.03.20
  • Accepted : 2016.04.16
  • Published : 2016.06.30
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Abstract

Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus, emphasizes the importance of improved access to early and accurate detection of T. vaginalis. In this study, a rapid and efficient loop-mediated isothermal amplification-based method for the detection of T. vaginalis was developed and validated, using vaginal swab specimens from subjects suspected to have trichomoniasis. The LAMP assay targeting the actin gene was highly sensitive with detection limits of 1 trichomonad and 1 pg of T. vaginalis DNA per reaction, and specifically amplified the target gene only from T. vaginalis. Validation of this assay showed that it had the highest sensitivity and better agreement with PCR (used as the gold standard) compared to microscopy and multiplex PCR. This study showed that the LAMP assay, targeting the actin gene, could be used to diagnose early infections of T. vaginalis. Thus, we have provided an alternative molecular diagnostic tool and a point-of-care test that may help to prevent trichomoniasis transmission and associated complications.

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References

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