• Title/Summary/Keyword: EXTN-1

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Sequential Sampling Plan for Aphis gossypii (Hemiptera: Aphididae) based on Its Intra-plant Distribution Patterns in Greenhouse Cucumber at Different Growth Stages (온실재배 오이의 생육단계별 목화진딧물의 주내 분포 특성에 기초한 축차표본조사법)

  • Chung, Bu-Keun;Song, Jeong-Heub;Lee, Heung-Su;Choi, Byeong-Ryul
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.401-407
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    • 2015
  • This study describes the development of a method for monitoring Aphis gossypii in greenhouse cucumber fields that was used during 2013 and 2014. The dispersion pattern of A. gossypii was determined by commonly used methods: Taylor's power law (TPL) and Iwao's patchiness regression (IPR). The sample unit was determined by linear regression analysis between mean density of sample unit versus whole plant. The optimum sample unit for different plant growth stages was two leaves (median and the lowest + 1 leaf) when the total number of leaves was less than nine, and three leaves (4th, 7th from canopy, and the lowest +1 leaf) when the total number of leaves was greater than nine. A. gossypii showed an aggregated distribution pattern, as the slopes of both TPL and IPR lines were greater than 1. TPL provided a better description of the mean-variance relationship than did IPR. The slopes and intercepts of TPL and IPR from leaf samples did not differ between the surveyed years. Fixed precision levels (D) for a sequential sampling plan were developed using Green's and Kuno's equations based on the number of aphid in a leaf sample. Green's method was more efficient than Kuno's to stop sampling. The number of samples needed to estimate the density of A. gossypii increased at higher D levels and lower mean densities. The cumulative number of aphids needed to stop sampling increased at higher D levels and with fewer plants sampled. Thus to estimate 10 aphids per leaf, 13 plants needed to be sampled, and the cumulative number of aphids to stop sampling was 131.

Effect of Skin Sooty and Decay Disease Control on ‘Niitaka’ Pear Fruit for Storage (신고배 저장중 과피얼룩 및 부패병에 대한 방제 효과)

  • Lee, Jung-Sup;Choi, Jin-Ho;Park, Jong-Han;Kim, Dae-Hyun;Han, Kyung-Sook;Han, You-Kyoung
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.230-235
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    • 2009
  • Postharvest skin sooty dapple and decay disease of pear fruit often originates at small stain symptoms that occurred during harvest and handling. Experiments were conducted to characterize the effect of timing of application of disease control materials, and to evaluate sequential postharvest applications of fungicides or fungicides and bio-control agents. Fungicides and bio-control agents were increasingly less effective when the period between harvest and application was prolonged. Thiabendazole (TBZ) applied to fruit without artificial wounding or inoculation effectively reduced skin sooty and decay disease when applied within 3 weeks or 6 weeks in 2 years of study. TBZ, Fludioxonil and pyrimethanil were effective in controlling skin sooty and decay disease at artificial wounds inoculated with Cladosporium tenuissimum up to 14 days after inoculation. Application of TBZ at harvest followed 3 weeks later by application of Fludioxonil was superior to application of TBZ at harvest alone. Two bacterial biocontrol agents reduced skin sooty and decay disease at pear wounds inoculated with C. tenuissimum up to 14 days after inoculation with C. tenuissimum, but were ineffective when applied at 28 days after inoculation. Of possible sequential arrangements of fungicide and bio-control treatments, application of the most effective material promptly after harvest generally resulted in the highest level of disease control.

Control of Colletotrichum acutatum and Plant Growth Promotion of Pepper by Antagonistic Microorganisms (길항균주를 이용한 고추탄저병균(Colletotrichum acutatum) 방제 및 식물생장촉진효과)

  • Han, Joon-Hee;Kim, Moon-Jong;Kim, Kyoung Su
    • The Korean Journal of Mycology
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    • v.43 no.4
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    • pp.253-259
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    • 2015
  • Anthracnose caused by Collectotrichum acutatum is the most devastating disease of pepper plants in Korea. In this study, we evaluated the effect of selected antagonistic bacteria on control of anthracnose and plant growth promotion of pepper plants under field conditions. Four different bacterial isolates used in the current study were isolated from the pepper rhizosphere (GJ01, GJ11) and tidal flat (LB01, LB14) in previous studies. Four bacterial isolates, together with a control strain (EXTN-1), showed antifungal activity against C. acutatum in a dual culture assay. To test for plant growth promotion effect, seedling vigor index and growth parameters of pepper were measured under field condition. As a result, all four bacterial isolates were effective for improving plant growth promotion. The strain GJ01 was the most effective in improving the seedling vigor on pepper, but the strain GJ11 in increasing the pepper fruit yield. The incidence of anthracnose was inhibited in the range of 63.2~72.5% by treatment of four bacterial isolates. The current study indicated that the four bacterial isolates could be used as potential biological control agents of anthracnose disease of pepper.

Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici

  • Elena, Volynchikova;Ki Deok, Kim
    • The Plant Pathology Journal
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    • v.39 no.1
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    • pp.123-135
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    • 2023
  • Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO4 solution (untreated control). In diluted (nutrient-deficient) V8 juice broth, the tested strain populations were maintained at >108 cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 106 cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.