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사료 내 Phytase 첨가가 산란계의 분뇨 배설량에 미치는 영향 (Effect of Dietary Phytase on the Excreta Excretion of Laying Hens)

  • 홍의철;나승환;유동조;김학규;박미나;정기철;추효준;박희두;정완태;황보종
    • 한국가금학회지
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    • 제36권3호
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    • pp.257-264
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    • 2009
  • 본 시험은 사료 내 phytase 첨가가 산란계의 분뇨 배설량 및 질소와 인의 배설량에 미치는 영향을 구명하기 위해 수행되었다. 시험에 사용된 닭은 1일령 ISA Brown종 산란계(평균체중 $44.7{\pm}1.5\;g$) 360수이며, 65주령에 걸쳐서 분뇨 배설량 및 55주령에서 phytase의 첨가에 따른 총 배설량과 질소와 인의 배설량에 미치는 영향을 조사하였다. 시험 사료는 옥수수-대두박 위주의 육계 사료를 육성기와 산란기로 분류하고, 육성기는 초생추(0~5주령), 중추(5~12주령), 대추(12~16주령)와 산란 전(16주령~초란), 산란기는 산란 초기(초란~32주령), 전기(32~45주령), 후기(45~55주령), 말기(55주령~)로 나누어 급여했다. Phytase의 첨가에 따른 55주령의 산란계 총 배설량 및 질소와 인의 배설량에 미치는 영향을 조사하기 위하여 후기 기초사료에 phytase(0, 300, 600 FTU/kg)를 첨가하여, 총 3처리구, 처리구당 10수씩 대조구와 유사한 체중별로 선별하여 완전임의 배치하였다. 산란계의 전 시험기간 중 평균 체중과 사료 섭취량, 음수량 및 분뇨 배설량은 각각 1,622 과 105.7 g, 187.2 mL 및 124.7 g/수/일로 조사되었다. Phytase의 첨가에 따른 분뇨 배설량은 건물(33.2, 31.2, 30.5 g/일) 및 질소(0.46, 0.42, 0.40 g/일)와 인(0.51, 0.49, 0.48 g/일)으로서 Phytase의 첨가에 따라 감소하였으며, phytase의 첨가량에 따른 차이는 없었다. 그러나 건물 분뇨 배설량에서 phytase 무첨가구와 300 FTU/kg phytase 첨가구간에는 유의적 차이가 없었다. 본 시험을 통하여 산란계의 총 배설량 및 질소와 인의 배설량을 조사하고, 단기간의 phytase 첨가도 산란계의 분뇨 배설량 및 질소와 인의 배설량을 감소시킬 수 있을 것으로 사료된다.

유산균(L. reuteri)의 첨가가 돼지의 생산성, 분과 결장내 미생물균총 및 육등급에 미치는 영향 (Effects of Dietary Supplementation of Lactobacillus reuteri on Performance of Swine, Fecal and Rectumal Microflora and Carcass Grade)

  • 손장호;김상호
    • 한국유기농업학회지
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    • 제13권2호
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    • pp.185-195
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    • 2005
  • Two hundred [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs were used in a 117-d growth assay (including four growth stages) to determine the effects of dietary supplementation of Lactobacillus reuteri on performance of swine, fecal and rectumal microflora and carcass grade. Pig diet was divided tow types, commercial diet (Control group) and supplemention of 0.1% Lactobacillus reuteri (Treatmental group). There was tend to increased in average daily gain (ADG) and feed efficiency (Feed/gain) in treatmental group than control group during the whole experimental period. The number of Lactobacilius spp. into rectum and feaces and carcass rate tended to increase in treatmental group than control group. Ammonia emission from excreta were decrease by supplemention of 0.1% Lactobacillus reuteri in feed (P<0.05). These results indicated that the dietary Lactobacillus reuteri were effective in performance, increasing of Lactobacilius spp. into rectum and feaces, decreasing ammonia emission from excreta, and it had also effective the carcass rate in swine.

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Assessing Nitrogen and Phosphorus in Excreta from Grower-finisher Pigs Fed Prevalent Rations in Vietnam

  • Vu, T.K.V.;Sommer, G.S.;Vu, C.C.;Jorgensen, H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권2호
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    • pp.279-286
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    • 2010
  • Livestock production in Vietnam is, as in most Asian countries, increasing rapidly and changing into specialized highly intensified operations. The volume of animal excreta generated exceeds the capacity of the operation land base and cannot be utilized efficiently. As a consequence, there is a loss of plant nutrients from livestock farms that causes environmental pollution. This study carried out a feed and excretion experiment measuring fecal characteristic, daily fecal production, daily nitrogen and phosphorous excretion from grower-finisher pigs fed prevalent rations in Vietnam. Furthermore, equations for assessing the excretion were tested, which can be used in farm models for optimal recycling of manure while focusing on reducing pollution. The results indicated that fecal production and nutrient excretion were affected by the different rations tested. This study showed that five selected equations for predicting excretion from grower-finisher pigs in Danish conditions can also be used with precision in Vietnamese pig farming systems. The equations have been proven valid and can, therefore, be used as a much needed tool for assessing fecal production and nitrogen in excreta on pig farms. The study also showed that about 12% of nitrogen excreted was emitted during housing. Waste water contains more than half of the nitrogen excreted, mainly in ammonium form which has a high potential for gaseous emission.

겉뿌린 목초종자의 정착에 관한 연구 II. 각종 증량재 피복이 파종목초의 발아 , 정착 및 수량에 미치는 영향 (Studies on Establishment of Over sown Pasture Seed II. Effect of coating materials on germination and yield of pasture species)

  • 이효원;김훈기;김창호
    • 한국초지조사료학회지
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    • 제10권1호
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    • pp.10-14
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    • 1990
  • The effects of coating materials on germination, establishment and herbage production were investigated in a laboratory and field over 1988-1989. For this purpose, four treatments of lime 50 % + briquet ash 50 %, briquet ash, dairy excreta and fowldung as coating material were applied to orchardgrass and red clover with meth ylcellulose. The germination rate of lime 50 % + briquet ash 50 % (70.6 %), briquet ash (84.0 %), dairy excreta (66.0 %) and fowldung (61.3 %) in orchardgrass were, showing lower than that of the control (86.6 %), respectively and the rate of red clover ranged from 59.0 to 18.6 %. Establishment in orchardgrass were from 2.0 to 0.9 %, showing lower than the control, whole a little higher except dairy excreta and fowldung treatments in red clover than the control. The DM yield of lime 50 % + briquet ash 50 % treatment was almost same to the control and other treatment showed lower yield compared with the yield of the control. The benefical effect of seed coating on field performance was minimal especially in terms of establishment and yield increase.

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$^{14}C$ Glycine-Glucose Melanoidin 과 양조간장의 대사 (Metabolism of $^{14}C$ Glycine-Glucose Melanoidin and Soybean Sauce)

  • 문갑순
    • 한국식품영양과학회지
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    • 제23권2호
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    • pp.333-339
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    • 1994
  • The metabolic transit of three samples( 14C glycine-glyucose melanoidin, glycine-glucose melanoidin and soybean sauce ) were studied on rats. The radioactivity of various organs and excreta intubated 14C glycine-glucose melanoidin on rats at various intervals(1, 3, 8, 12, 24, 48, 72 and 96 hrs were detected . And the brown pigment contents and hydrogen donating activities in the excreta which is obtained from three samples were detected during the 7 days after intubated. The total amount of 14C excreted in the fecese were 53% meaning that the rest of 47% melanoidin seemed to be retained in the body or metabolized . The radioactive compound showed a small retention in the liver and kidney. The brown pigment contents and hydrogen donating activities in the urine and feces increased proportionally to the activity of 14C. When the soybean sauce and glycine glucose melanoidin were intubated, the brown pigment contents excreted in the feces were found to be the highest after 1 st day of intubation. In the urine, the model melanoidin was excreted mostly after 3 days of intubation. The brown pigment contents and hydrogen donating activities of three samples of excreta agreed with each other. The soybean sauce retained longer than model melanoidin in the body is telling that it might have antioxidative activity in vivo.

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Bacillus subtilis PNG-4의 단독 및 Lactobacillus acidophilus와의 혼합 사용이 산란계의 건물소화율, 혈액성상 및 계분의 악취 발생에 미치는 영향 (Effect of Bacillus subtilis PNG-4 with or without Lactobacillus acidophilus on malodorous gas emission of excreta in laying hens)

  • 김이수;차상우;조성경;김성복;이봉덕;이수기
    • 농업과학연구
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    • 제37권1호
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    • pp.53-60
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    • 2010
  • Two experiments were conducted to investigate the effect of probiotics on the malodor removal. In experiment 1, dietary effects (several malodorous gas concentration of excreta, dry matter metabolizability, and blood profiles) were determined using laying hens. A total of 30 Hy-line brown layers, 68-wk of age, were randomly allocated into 5 groups with 3 replicates of 2 birds each. The treatments were probiotics free, 0.2% and 0.4 % addition of mixed probiotics (Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS), and 0.2 and 0.4 % addition of single probiotics (Bacillus subtilis PNG-4). In experiment 2, the effects of mixing of probiotics into the excreta on the malodorous gas removal was investigated. There were three treatments (probiotics free, Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS, and Bacillus subtilis PNG-4) with three replicates. The malodorous gas concentrations were detected at 0, 3, 7 and 14 day of incubation. In experiment 1, ammonia concentration was significantly decreased by feeding mixed probiotics at 14th day of incubation. However, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan were not significantly affected by mixed probiotics. Dry matter metabolizability was significantly increased by feeding probiotics, but no significant differences between single and mixed probiotics. There was no significant differences in blood profiles. In experiment 2, mixing of probiotics into the excreta did not affect the concentration of ammonia, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan. Therefore, these experiments suggested that Bacillus subtilis PNG-4 + Lactobacillus acidophilus LAS supplementations could improve ammonia gas removal, and dry matter metabolizability in layers. Also, decrease of ammonia concentration was higher in mixed probiotics group compare to the single probiotics group. On the other hand, mixing of probiotics into the excreta appeared not to be a useful method.

양계사료의 TME측정에 영향하는 요인에 관한 시험 II. 사료투입량 및 사료급여형태가 옥수수와 대두박의 대사 에너지가에 미치는 영향 (Factors Affecting True Metabolizable Energy Determination of Poultry Feedingstuffs Effect of level of feed input and diet types on apparent and true metabolizable energy of corn and soybean meal.)

  • 이영철;강도환
    • 한국가금학회지
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    • 제10권1호
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    • pp.46-52
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    • 1983
  • 사료급여량이 옥수수와 대두박의 TME(AM $E_{F}$ )와 TME(AM $E_{FC}$ )가에 미치는 영향과 사료의 형태(단미와 배합)가 옥수수의 TME가에 미치는 영향을 검토하고자 실시한 결과는 다음과 같다. 1. 옥수수 대두박의 AME, AM $E_{F}$ 가는 공히 각급이수준간 현저한 유의차(P<.05)가 있었으며, 특히 50mg 급이수준에서 옥수수, 대두박 공히 가장 높았다. 반면 옥수수의 AME 가는 50과 75mg 합이수준간에는 유의차(P>.05)가 없었다. 2. 옥수수의 TME와 AM $E_{FC}$ 가는 각 급이수준간 유의차가 없었던(P>.05) 반면 대두박은 각 수준간 현저한 차가(P<.05) 있었다. 5 mg 급이수준에서 대두박의 AM $E_{FC}$ TME 가가 가장 높은 사실은 특히 대두박의 경우에는 TME 측정상 문제점이 있음을 지적하는 것이다. 3. 단미 또는 배합사료 형태로 측정한 옥수수의 TME가는 두 형태간 유의성은 없었다. (P>.05)

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Effect of dietary protein levels and age on growth performance, total excreta and nitrogen balance of laying hens during the growing phase

  • Jeseok Lee;Myunghwan Yu;Elijah Ogola Oketch;Shan Randima Nawarathne;Yu Bin Kim;Nuwan Chamara Chathuranga;Venuste Maniraguha;Bernadette Gerpacio Sta. Cruz;Eunsoo Seo;Haeeun Park;Hyunji Choi;Dong Jo Yu;Jung-Kon Kim;Jung Min Heo
    • 농업과학연구
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    • 제51권2호
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    • pp.193-203
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    • 2024
  • This study examined the effect of a dietary protein level and age on growth performance, total excreta, and nitrogen balance in laying hen during the growing period for 18 weeks. A total of 96 Hy-Line Brown laying hens were divided into two treatments according to the experimental diets, with each group consisting of six replicates. The experimental diets included: 1) an adequate metabolizable energy and crude protein diet formulated to meet the age and breed specification for Hy-Line Brown containing 21, 18, 16, and 14% dietary CP during 0 to 6, 6 to 12, 12 to 15, 15 to 18 weeks, respectively (CON); 2) 2% CP deficiency from CON diet containing 19, 16, 14, 12% dietary CP during 0 to 6, 6 to 12, 12 to 15, 15 to 18 weeks, respectively (LP). Growth performance, total excreta, and nitrogen balance were investigated for 18 weeks every 3 weeks, and two-way ANOVA was used for statistical analysis of data. The LP group had lower total excreta and N intake than CON group in the whole period of the experiment (p < 0.001). Moreover, birds in the LP group showed lower N excretion than the CON group (p < 0.05). However, the CON group exhibited higher BW, ADG than the LP group (p < 0.001). Application of low-protein diet to laying hens during the growing period reduced N excretion, which contribute to the sustainable development of the laying hen industry by minimizing environmental pollution.

뽕잎과 잠분 추출물 급여가 육계의 생산성, 혈액 성상 및 맹장 내 미생물군에 미치는 영향 (Effects of Dietary Supplementation of Mulberry Leaves and Silkworm Excreta Extracts on Weight Performance, Blood Characteristics and Cecal Microflorae of Chicks)

  • 한상훈;김동욱;지상윤;홍성구;김상호;이희삼
    • 한국잠사곤충학회지
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    • 제50권2호
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    • pp.150-160
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    • 2012
  • 본 연구는 뽕잎과 잠분의 에탄올 추출물을 첨가 급여하였을 경우, 육계의 생산성 및 저장성에 미치는 영향을 규명하기 위하여, 부화 1일령 육계 수평아리(Ross) 240수를 공시하여 항생제 무첨가군, 항생제 첨가군을 대조군으로 하고, 뽕잎 에탄올 추출물 0.1%와 1% 첨가군, 잠분 에탄올 추출물 0.1%와 1% 첨가군 등 6 처리군으로 분류하고 5주간 사육하여 육계의 생산성, 혈액 생화학, 혈액 내 항산화 관련 인자, 계육의 저장성, 맹장 내 미생물 균총 등에 대한 조사를 수행하였다. 뽕잎 추출물 및 잠분 추출물 첨가 급여 시 육계의 증체량에서는 대조군들에 비해 유의한 수준의 차이를 보이지 않았고 및, 사료 요구율은 항생제 첨가 사료를 사용한 대조구에 비해 개선되어 생산성 향상 효과를 관찰할 수 있었고, 혈액 총항산화 활성을 나타내는 TEAC 수치 및 혈액 내 지질과산화물인 malondialdehyde(MDA) 함량을 조사한 결과 대조군에 비해 잠분 추출물 첨가 급여 시 혈액 성상의 긍정적 영향을 관찰할 수 있었다(P = 0.001). 또한 큰 차이는 아니었지만, 저장 기간별 지질산패도를 조사한 결과 뽕잎 추출물 및 잠분 추출물 첨가 급여 시 저장기간에 따른 지질 산화가 다소 감소하는 경향을 보였는데, 사육 종료 후 혈액을 채취하여 혈중 MDA 농도를 측정한 결과는 SEA 실험군에서 가장 낮은 값을 보였지만, 10일 동안 총 증가한 MDA의 농도는 MLB군에서 가장 낮았다($P=4.417{\times}10^{-3}$). 맹장 내 미생물 균총에 대한 영향은 잠분 추출물 첨가 급여 시 항생제 무첨가군에 비해 총 균수의 감소가 유의한 수준에서 관찰되었고($P=6.462{\times}10^{-5}$), coliform bacteria의 경우 항생제 무첨가군에 비해 유의한 수준에서 ($P=1.182{\times}10^{-7}$) 감소하는 경향을 보여주었다. Salmonella sp.에 대한 생장저해 효과는 전실험군에서 유의한 차이를 보이지 않았다(P = 0.343). 흥미로운 점은 다른 처리군들과 달리 MLB 실험군의 경우 무항생제 실험군에 비해 유의한 수준은 아니지만 Lactobacillus sp.의 성장을 향상시키는 경향을 보였다. 따라서 본 연구의 결과는 육계용 사료에 있어서 뽕잎과 잠분의 에탄올 추출물 첨가 급여가 생산성을 저하시키지 않으면서도 혈액성상에 유익한 영향을 제공함으로써 계육의 품질 개선에 긍정적인 효과를 제공함은 물론 항생제 대체제로서의 효과를 제공할 수 있을 것으로 사료되며, 실용화 될 경우 잠업 농가의 수익성 개선에 도움이 될 것으로 기대된다.