DOI QR코드

DOI QR Code

Effect of Dietary Phytase on the Excreta Excretion of Laying Hens

사료 내 Phytase 첨가가 산란계의 분뇨 배설량에 미치는 영향

  • 홍의철 (농촌진흥청 국립축산과학원) ;
  • 나승환 (농촌진흥청 국립축산과학원) ;
  • 유동조 (농촌진흥청 국립축산과학원) ;
  • 김학규 (농촌진흥청 국립축산과학원) ;
  • 박미나 (농촌진흥청 국립축산과학원) ;
  • 정기철 (농촌진흥청 국립축산과학원) ;
  • 추효준 (농촌진흥청 국립축산과학원) ;
  • 박희두 (농촌진흥청 국립축산과학원) ;
  • 정완태 (농촌진흥청 국립축산과학원) ;
  • 황보종 (농촌진흥청 국립축산과학원)
  • Published : 2009.09.30

Abstract

This work was conducted to investigate the effects of phytase on N and P excretion of laying hens excreta. Three hundred sixty ISA Brown layers were selected to investigate excreta excretion for 65 weeks and to investigate the effect of phytase on total excretion and N and P excretion of layers from 55 weeks. The experimental diets were fed the starter (0~5 wk), grower (5~12 wk), developer (12~16 wk), prelay diets (16 wk~first egg), layer diets for starting period (first egg~32 wk), middle (32~45 wk), finishing diets (45~55 wk, 55 wk~). Thirty ISA Brown layers were selected to investigate the effect of phytase supplementation on total N and P excretion of layers at 55 weeks and assigned randomly to 3 treatments groups (10 birds/treatment) and phytase was added to basal diets at 300 and 600 FTU/kg. Average body weight, feed intake, water intake, and excreta excretion were 1,622, 105.7, 187.2 and 124.7 g/bird/day, respectively. Excreta of birds fed phytase were DM (33.2, 31.2, 30.5 g/day), N (0.46, 0.42, 0.40 g/day) and P (0.51, 0.49, 0.48 g/day) and reduced as dietary phytase increased, Finally, dietary phyase can reduce the N and P excretion, but the amount of excreta was not different depending from the phytase addition in layers. This work investigated the N and P excretion of laying hens excreta and were considered that dietary phytase can reduce the N and P excretion for short period.

본 시험은 사료 내 phytase 첨가가 산란계의 분뇨 배설량 및 질소와 인의 배설량에 미치는 영향을 구명하기 위해 수행되었다. 시험에 사용된 닭은 1일령 ISA Brown종 산란계(평균체중 $44.7{\pm}1.5\;g$) 360수이며, 65주령에 걸쳐서 분뇨 배설량 및 55주령에서 phytase의 첨가에 따른 총 배설량과 질소와 인의 배설량에 미치는 영향을 조사하였다. 시험 사료는 옥수수-대두박 위주의 육계 사료를 육성기와 산란기로 분류하고, 육성기는 초생추(0~5주령), 중추(5~12주령), 대추(12~16주령)와 산란 전(16주령~초란), 산란기는 산란 초기(초란~32주령), 전기(32~45주령), 후기(45~55주령), 말기(55주령~)로 나누어 급여했다. Phytase의 첨가에 따른 55주령의 산란계 총 배설량 및 질소와 인의 배설량에 미치는 영향을 조사하기 위하여 후기 기초사료에 phytase(0, 300, 600 FTU/kg)를 첨가하여, 총 3처리구, 처리구당 10수씩 대조구와 유사한 체중별로 선별하여 완전임의 배치하였다. 산란계의 전 시험기간 중 평균 체중과 사료 섭취량, 음수량 및 분뇨 배설량은 각각 1,622 과 105.7 g, 187.2 mL 및 124.7 g/수/일로 조사되었다. Phytase의 첨가에 따른 분뇨 배설량은 건물(33.2, 31.2, 30.5 g/일) 및 질소(0.46, 0.42, 0.40 g/일)와 인(0.51, 0.49, 0.48 g/일)으로서 Phytase의 첨가에 따라 감소하였으며, phytase의 첨가량에 따른 차이는 없었다. 그러나 건물 분뇨 배설량에서 phytase 무첨가구와 300 FTU/kg phytase 첨가구간에는 유의적 차이가 없었다. 본 시험을 통하여 산란계의 총 배설량 및 질소와 인의 배설량을 조사하고, 단기간의 phytase 첨가도 산란계의 분뇨 배설량 및 질소와 인의 배설량을 감소시킬 수 있을 것으로 사료된다.

Keywords

References

  1. AOAC 2000 Official Method of Analysis. 17th ed. Association of Official Analytical Chemists. Washington DC USA P1-43
  2. Boucque ChV, Fiems LO, Sommer M, Cottyn BG, Buysse FX 1987 Effects of the beta-agonist cimaterol on growth, food efficiency and carcass quality of finishing Belgian White-Blue Bulls. In: J.P. Hanrahan (Editor), Beta-agonists and their Effects on Animal Growth and Carcass Quality. Commission of the European Communities. Elsevier Applied Science UK pp 93-10
  3. Camden BJ, Morel PCH, Thomas DV, Ravindran V, Bedford MR 2001 Effectiveness of exogenous microbial phytase in improving the bioavailabilities of phosphorus and other nutrients in maize-soya-bean meal diets for broilers. Anim Sci 73:289-297 https://doi.org/10.1017/S1357729800058264
  4. Chilliard Y 1989 Long term effects of recombinant bovine somatotrophin (rBST) on dairy cow performances: a review. In: K. Serjrsen, M. Vestergaard and A. Neimann-Sorensen (Editors), Use of Somatotrophin in Livestock Production, Commission of the European Communities. Elsevier Scientific UK
  5. Correll DL 1999 Phosphorus: a rate limiting nutrient in surface waters. Poult Sci 78:674-682 https://doi.org/10.1093/ps/78.5.674
  6. Cowieson AJ, Acamovic T, Bedford MR 2004 The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. Br Poult Sci 45(1): 101-108 https://doi.org/10.1080/00071660410001668923
  7. Cowieson AJ, Acamovic T, Bedford MR 2006 Phytic acid and phytase: Implications for protein utilization by poultry. Poult Sci 85:878-885 https://doi.org/10.1093/ps/85.5.878
  8. Creswell O, Swick RA 2001 Formulating with digestible amino acids (part 3). Asian Poul Magaz (May/June) 21-22. 24
  9. Cromwell GL, CoVey RD 1995 Nutrient management from feed to Weld. In: Proceedings of World Pork Expo., Des Moines IA USA pp. 13-31
  10. Dilger RN, Onyango EM, Sands JS, Adeola O 2004 Evaluation of microbial phytase in broiler diets. Poult Sci 83:962-970 https://doi.org/10.1093/ps/83.6.962
  11. Duncan DB 1995 Multiple range and multiple F tests. Bio-metrics 11:1-42
  12. Eeckhout W, de Paepe M 1994 Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Anim Feed Sci Technol 47:19-29 https://doi.org/10.1016/0377-8401(94)90156-2
  13. Godoy S, Chicco C, Meschy F, Requena F 2005 Phytic phosphorus and phytase activity of animal feed ingredients. Interciencia 30:24-28
  14. Hanrahan TJ 1989. Use of somatotrophin in livestock production: growth in pigs. In: K. Serjrsen, M. Vestergaard and A. Neimann-Sorensen (Editors), Use of Somatotrophin in Livestock Production, Commission of the European Communities. Elsevier Scientific UK
  15. Harada Y 1996 Animal manure recycle systems and its utilization in Japan. Proceedings of the 8th AAAP Animal Science Congress 99-108
  16. Kirby LK, Nelson TS 1988 Total and phytate phosphorus content of some feed ingredients derived from grains. Nutr Reports Int 37:277-280
  17. Mahan DC, Howes D 1995 Environmental aspects with particular emphasis on hosphrus, selenium, and chromium in livestock feeds. In: OYcial Proceedings 13th Annual PaciWc Northwest Animal Nutrition Conference, October 10.12, Marriott Hotel Portland OR USA, pp. 75-89
  18. Nahm KH 2007 Feed formulations to reduce N excretion and ammonia emission from poultry manure. Bioresource Technology 98:2282-2300 https://doi.org/10.1016/j.biortech.2006.07.039
  19. Nelson TS, Ferrara LW, Storer NL 1968 Phytate phosphorus content of feed ingredients derived from plants. Poult Sci 47:11372-11374
  20. North MO 1972 Commercial Chicken Production Manual. The Avian Publishing Company Inc
  21. Ostrander CE 1965 Poultry manure disposal. Amer Soc Agr Eng Trans 8(1):105-106 https://doi.org/10.13031/2013.40442
  22. Ouyang J, Yan M, Kong D, Xu L 2006. A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei. Biotechnol J 1(11):1266-74 https://doi.org/10.1002/biot.200600103
  23. Paik I 2003 Application of phytase, microbial or plant origin, to reduce phosphorus excretion in poultry production. Asian-Aust J Anim Sci 16:124-135 https://doi.org/10.5713/ajas.2003.124
  24. Ravindran V, Morel PCH, Partridge GG, Hruby M, Sands JS 2006 Influence of an E. coli-derived phytase on nutrient utilization in broiler starter fed diets containing varying concentrations of phytic acid. Poult Sci 85:82-89 https://doi.org/10.1093/ps/85.1.82
  25. Ravindran V, Ravindran G, Sivalogan S 1994 Total and phytate phosphorus contents of various foods and feedstuffs of plant origin. Food Chem 50:133-136 https://doi.org/10.1016/0308-8146(94)90109-0
  26. Rutherfurd SM, Chung TK, Morel PCH, Moughan PJ 2004 Effect of microbial phytase on ileal digestibility of phytate phophorus, total phosphorus, and amino acids in a low-phosphorus diet fro broilers. Poult Sci 83:61-68 https://doi.org/10.1093/ps/83.1.61
  27. SAS 1999 SAS suer guide. release 6.11 edition. SAS Inst Inc Cary NC USA
  28. Selle PH, Ravindran V 2007 Microbial phytase in poultry nutrition. Anim Feed Sci Tech 135:1-41 https://doi.org/10.1016/j.anifeedsci.2006.06.010
  29. Selle PH, Ravindran V, Caldwell RA, Bryden WL 2000 Phytate and phytase: sonsequences for protein utilisation. Nutr Res Rev 13:255-278 https://doi.org/10.1079/095442200108729098
  30. Selle PH, Walker AR, Bryden WL 2003 Total and phytate-phosphorus contents and phytase activity of Australian sourced feed ingredients for pigs and poultry. Aust J Expt Agric 45: 475-479 https://doi.org/10.1071/EA02155
  31. Sharpley A 1999 Agricultural phosphorus, water quality and poultry production: are they compatible? Poult Sci 78:660-673 https://doi.org/10.1093/ps/78.5.660
  32. Shirley RB, Edwards HM 2003 Graded levels of phytase past industry standards improves broiler performance. Poult Sci 82:671-680 https://doi.org/10.1093/ps/82.4.671
  33. Simons PCM, Versteegh HAJ, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MGE, Beudeker RF, Verschoor GJ 1990 Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br J Nutr 64:525-540 https://doi.org/10.1079/BJN19900052
  34. Tyrrell HF, Brown ACG, Reynolds PJ, Haaland GL, Bauman DE, Peel CJ, Steinhour WD 1988 Effect of bovine somatotrophin on metabolism of lactating dairy cows: energy and nitrogen utilisation as determined by respiration calorimetry. J Nutr 118:1024-1030 https://doi.org/10.1093/jn/118.8.1024
  35. Van Weerden EJ 1987 Effects of clenbuterol on nitrogen deposition and carcass composition in castrated male pigs. In: J.P. Hanrahan (Editor), Beta-agonists and their Effects on Animal Growth and Carcass Quality. Commission of the Eu ropean Communities. Elsevier Applied Science UK pp. 152-162
  36. Viveros A, Conteno C, Brenes A, Canales R, Rozano A 2000 Phytase and acid phosphatase activities in plant feedstuffs. J Agric Food Chem 48:4009-4013 https://doi.org/10.1021/jf991126m
  37. Zyla K, Koreleski J, Swiatkiewicz S, Piironen J, Ledoux DR 2001 Influence of supplemental enzymes on the performance and phosphorus excretion of broilers fed wheat-based diets to 6 weeks of age. Anim Feed Sci Technol 89:13-118
  38. 日本中央畜産會. 1989. '家畜尿汚水の處理利用技術と事例', 中央畜産會
  39. 齊藤守2001 ニワトリおよびブタから環境負荷物質の低減化に關する榮養飼料學的硏究の動向. Anim Sci J 72(8):177-199
  40. 농촌진흥청/농업과학기술원 1999 친환경농업을 위한 가축 분뇨 퇴비.액비 제조와 이용
  41. 한국사양표준(가금) 2007 농림부. 농촌진흥청 국립축산과학원
  42. 황보종, 안정현, 정완태, 오상집, 이현정, 김원, 이선업, 홍의철 2007b Phytase 수준별 급여가 육계의 생산성, 인의 배설과 흡수 및 회장과 분에서 소화율에 미치는 영향. 한국가금학회지 34(3):207-215 https://doi.org/10.5536/KJPS.2007.34.3.207
  43. 황보종, 안정현, 정완태, 오상집, 이현정, 김학규, 이선업, 홍의철 2007a Phytase 첨가가 산란계의 생산성, 난질, 질소와 인의 배설량 및 외장 소화율에 미치는 영향. 한국가금학회지 34(2):119-128 https://doi.org/10.5536/KJPS.2007.34.2.119