• Title/Summary/Keyword: ERE$

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ESTABLISHMENT OF BIOASSAY TO DETECT ESTROGENIC FLAVONOIDS USING STABLE MCF-7-ERE CELL AND MCF-7 CELL PROLIFERASTION ASSAY

  • Joung, Ki-Eun;Kim, Yeo-Woon;Sheen, Yhun-Yhong
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.11a
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    • pp.113-113
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    • 2001
  • Stable MCF-7-ERE cells, in which pERE-Luc reporter gene has been stably integrated into the genome of the MCF-7 cells, were used to detect the estrogenic activity of various dietary flavonoids in either pure chemical or mixtures. Estradiol (E2) induced luciferase activity in dose dependent manner and this activity was inhibited by tamoxifen (Tam) concomitant treatment. A large series of flavonoids showed estrogenic activities, corresponding to EC5O values between 0.2 and 9 microM and their mixtures didn't show additive or synergistic effects. And we could find some structure and activity relationship. First, 4-methoxylation and catechol structure decreased estrogenic activities. Second, hydroxylation of 3 position reduced estrogenic effect. Third glycosides of flavonoids showed weak estrogenic activity or no activity. Interestingly, when tested at high concentrations, genistein, kaempferol, biochanin A and chrysin elicited luciferase induction higher than that of the maximum induction by estradiol. And these effects of genistein and kaempferol could not be fully inhibited with tamoxifen

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The Characteristics of Mathematical Errors & Discourse in a Supplementary Class for the Migrant Students from North Korea (탈북학생들을 위한 수학 보충학습에서 담론 속에 나타난 오류유형과 담론의 특성)

  • ChoiKoh, Sang-Sook
    • Journal of the Korean School Mathematics Society
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    • v.15 no.1
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    • pp.53-80
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    • 2012
  • This study was designed to find the characteristics of mathematical errors and discourse in simultaneous equations and inequalities for migrant students from North Korea. 5 sample students participated, who attended in an alternative school for the migrant students from North Korea at the study in Seoul, Korea. A total of 8 lesson units were performed as an extra curriculum activity once a week during the 1st semester, 2011. The results indicated that students showed technical errors, encoding errors, misunderstood symbols, misinterpreted language, and misunderstood Chines characters of Koreans and the discourse levels improved from the zero level to the third level, but the scenes of the third level did not constantly happen. Nevertheless, the components of discourse, explanation & justification, were activated and as a result, evaluation & elaboration increased in ERE pattern on communication.

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Gait-based Human Identification System using Eigenfeature Regularization and Extraction (고유특징 정규화 및 추출 기법을 이용한 걸음걸이 바이오 정보 기반 사용자 인식 시스템)

  • Lee, Byung-Yun;Hong, Sung-Jun;Lee, Hee-Sung;Kim, Eun-Tai
    • Journal of the Korean Institute of Intelligent Systems
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    • v.21 no.1
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    • pp.6-11
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    • 2011
  • In this paper, we propose a gait-based human identification system using eigenfeature regularization and extraction (ERE). First, a gait feature for human identification which is called gait energy image (GEI) is generated from walking sequences acquired from a camera sensor. In training phase, regularized transformation matrix is obtained by applying ERE to the gallery GEI dataset, and the gallery GEI dataset is projected onto the eigenspace to obtain galley features. In testing phase, the probe GEI dataset is projected onto the eigenspace created in training phase and determine the identity by using a nearest neighbor classifier. Experiments are carried out on the CASIA gait dataset A to evaluate the performance of the proposed system. Experimental results show that the proposed system is better than previous works in terms of correct classification rate.

Comparative Estrogenic Effects of Yak-Kong and Soy Bean on the Proliferation of Human Osteoblastic Cell Line, MG-63 (MG 63 조골세포에서 약콩과 대두의 천연 에스트로겐 효과 비교)

  • 조윤희;박수진;신호정;장기효;강순아;조여원
    • Journal of Nutrition and Health
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    • v.34 no.8
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    • pp.905-911
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    • 2001
  • Phytoestrogens, especially soy-derived isoflavones, are receiving great scrutiny as a food supplement for preventing hormone dependent diseases such as cardiovascular diseases, cancer, and osteoporosis. These beneficial effects of phytoestrogens are caused by functioning as partial agonists or antagonists of estrogens. In contrast to the common usage of soy bean, Yak-kong(Rhynchosia Molubilis ; ) has been used as supplements of estrogen fir preventing postmenopausal osteoporosis in Oriental medicine. To investigate estrogenic effects of Yak-kong and soy bean on the proliferation of MG-63 osteoblastic cells, each bean was extracted with 70% methanol and dried by freeze-drying. Yak-kong treatment of MG-63 cells resulted in an increase of cell proliferation to a maximum of 76% compared to 68% of soy bean treatment. Treatment of MG-63 cells with Yak-kong extract also resulted in an increase of transactivation of an ERE(estrogen response element)-luciferase reporter plasmid and IGF-I expression selectively. Despite increased effects of both bean treatments on the expression of estrogen receptor $\alpha$(ER$\alpha$) and $\beta$(ER$\beta$), soy bean treatment decreased transactivation of an ERE-luciferase reporter plasmid and did not further enhance IGF-I expression. Together, our data demonstrates that the greater estrogenic response of Yak-kong extract for MG-63 cell proliferation is mediated by ER derived transactivation of ERE and selective induction of IGF-I expression.

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Economic Ripple Effect of the TKR on the Logistics Industry

  • KIM, Sun-Ju
    • Journal of Distribution Science
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    • v.19 no.3
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    • pp.25-34
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    • 2021
  • Purpose: The purpose of this study is to analyze the economic ripple effect(ERE) of logistics industry by construction of Trans-Korea Railway (TKR) and present policy measures to minimize the economic loss of South Korea (SK). Research design, data and methodology: As the analysis method, exponential smoothing was used for demand forecasting, Input-Output analysis was used to estimate the economic ripple effect coefficient, and scenario analysis was used to an efficient way to invest in TKR to minimize SK's economic losses. Results: 1) the production(logistics fares) of TKR for 10 years after its completion is about 11.42 trillion won in positive relations, and 26.89 billion won in negative relations. 2) the ERE of SK in positive relations is 24.32 trillion won in production inducement effect, 8.1 trillion won in value-added inducement effect, 3.54 trillion won in import inducement effect, and 70,930 persons in employment inducement effect. But the ERE was insufficient in the negative relations. 3) SK's efficient investment method is providing materials and equipment by SK and building the TKR by North Korea in positive inter-Korea relations. Conclusions: For the successful operation of TKR, international cooperation, legalization and stable peace settlement on the Korean Peninsula are required.

STUDY OF RESVERATROL AND ITS DERIVATIVES ON THE REGULATION OG GENE EXPRESSION IN MCF-7 CELLS TRANSFECTED WITH EITHER pERE-LUC OR phCYP1A1-LUC

  • Joung, Ki-Eun;Kim, Yeo-Woon;Sheen, Yhun-Yhong
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.11a
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    • pp.111-111
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    • 2001
  • Resveratrol (trans-3,4',5-trihydroxystilbene), which is a polyphenolic compound found in a variety of plants such as grapes and wine, has been reported to have a variety of anti-inflammatory, anti-platelet, and anti-carcinogenic effects. Recently resveratrol of was reported to serve as an estrogen agonist in MCF-7 cells Based on its structural similarity to diethylstilbestrol, a synthetic estrogen, we examined whether resveratrol and its derivatives might be estrogenic using stable MCF-7-ERE cells. Resveratrol functioned as a superagonist at high concentrations (i.e., produced a greater maximal transcriptional response than estradiol) Among the resveratrol derivatives, 10 compounds showed significant estrogenic activity.

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STUDY OF RESVERATROL AND ITS DERIVATIVES ON THE REGULATION OF GENE EXPRESSION IN MCF-7 CELLS TRANSFECTED WITH EITHER pERE-LUC OR pCYP1A1-LUC

  • Joung, Ki-Eun;Kim, Yeo-Woon;Sheen, Yhun-Yhong
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.10a
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    • pp.161-161
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    • 2001
  • Resveratrol (trans-3, 4', 5-trihydroxystilbene), which is a polyphenolic compound found in a variety of plants such as grapes and wine, has been reported to have a variety of anti-inflammatory, anti-platelet, and anti-carcinogenic effects. Recently resveratrol was reported to serve as an estrogen agonist in MCF-7 cells Based on its structural similarity to diethylstilbestrol, a synthetic estrogen, we examined whether resveratrol and its derivatives might be estrogenic using stable MCF-7-ERE cells. (omitted)

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STRUCTURE OF IDEMPOTENTS IN POLYNOMIAL RINGS AND MATRIX RINGS

  • Juan Huang;Tai Keun Kwak;Yang Lee;Zhelin Piao
    • Bulletin of the Korean Mathematical Society
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    • v.60 no.5
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    • pp.1321-1334
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    • 2023
  • An idempotent e of a ring R is called right (resp., left) semicentral if er = ere (resp., re = ere) for any r ∈ R, and an idempotent e of R∖{0, 1} will be called right (resp., left) quasicentral provided that for any r ∈ R, there exists an idempotent f = f(e, r) ∈ R∖{0, 1} such that er = erf (resp., re = fre). We show the whole shapes of idempotents and right (left) semicentral idempotents of upper triangular matrix rings and polynomial rings. We next prove that every nontrivial idempotent of the n by n full matrix ring over a principal ideal domain is right and left quasicentral and, applying this result, we can find many right (left) quasicentral idempotents but not right (left) semicentral.