• Journal of Sensor Science and Technology
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• v.28 no.6
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• pp.355-359
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• 2019

Exosomes, known as nanoscale extracellular vesicles in the range of 30-150 nm, are known to contain clinically significant information. However, there is still insufficient information on exosomal membrane proteins for cancer diagnosis. In this work, we investigated the characteristics of the membrane proteins of exosomes shed by cultured breast cancer cell lines using a surface plasmon resonance (SPR) spectroscopy and pre-activated alkanethiols modified sensor chips. The antibodies of breast cancer biomarkers such as MCU-16, EpCAM, CD24, ErbB2, and CA19-9 were immobilized on the pre-activated alkanethiols surfaces without any activation steps. The purified exosomes were loaded onto each antibody surface. The affinity rank of the antibody surfaces was decided by the relative capture efficiency factors for the exosomes. In addition, an antibody with a relative capture efficiency close to 100% was tested with exosome concentration levels of 10⁴/µl, 10⁵/µl, and 10⁶/µl for quantitative analysis.

• Journal of Chest Surgery
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• v.12 no.4
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• pp.361-364
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• 1979

The first Mitral Commissurotomy was performed for tight mitral stenosis on March 1957. The patient was at that time 22-year-old male, student. The longest follow 9p for 22 years and 8 months has been obtained. During the follow up period, late deterioration due to restenosis developed 4 years after initial good result and reoperation was succeeded by transventricular Mitral Valvotomy with Tubb`s ilator on April 1964. The possible cause of restenosis was attributed to recurrent rheumatic activity. After more than 13 years long-good life following 2nd operation, Endocarditis such as episode of high fever & chill intermittently followed by mild fever and night sweat, I t. tibial artery embolization and rupture of aortic cusp. At present, patient complained of no subjective symptom, enjoying ordinary life {NYHA II]. Blood pressure has been 110/50-60 mmHg, trivial diastolic murmur at apex and moderate degree of mechanical murmur on diastole at Erb`s rea. Neither signs of RVH for mitral stenosis nor sign of LVH. ST-T change for aortic regurgitation appeared yet during last 2 yrs. The patient`s are for prevention of Rheumatic activity and development of endocarditis is important for obtaining the better long-term result.

• Proceedings of the KIEE Conference
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• 2004.07e
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• pp.99-102
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• 2004

21세기 정보화시대에 맞추어, 도시철도운영기관의 전략적 발전과 도시철도의 정보화 기술 축적 및 표준화된 정보 인프라 구축의 일환으로 도시철도 시설물 유지보수체계를 표준화하고 정보화시스템을 구축중에 있다. 이중, 도시철도 통신 설비의 효율적인 유지보수를 위해서 유지보수체계의 표준화 및 정보화시스템 구축을 통한 실시간 정보의 구축 및 기존 유지보수작업 이력정보의 관리가 매우 중요하다. 본 연구에서는 도시철도 통신 설비의 효율적인 유지관리를 위한 데이터베이스 설계 및 구축방안에 대하여 제시하였다. 통신 설비의 데이터베이스 구축을 위하여 표준분류체계를 수립하였고, 관리항목 및 유지보수작업항목을 표준화하였으며, 유지 보수업무절차를 표준화하고 도면 및 각종 문서양식을 표준화하였다. 통신 설비의 데이터베이스는 개체관계모델(ERB)을 사용하여 설계하였고 특히, 통신 설비는 위치정보와 밀접한 관계를 가지고 있으므로 일반 데이터 외에 GIS용 공간 데이터베이스를 구축하여 서로 연관된 Key를 가지고 상호조회가 가능하도록 설계하였다. 그리고, 통신 설비에 대한 기초 데이터를 구축하기 위한 방안으로 엑셀조사 양식을 통하여 방대한 기초 자료를 작성하고, $SQL^*$Loader 등의 데이터 변환프로그램을 활용하여 데이터베이스에 일괄 등록되도록 하는 방안을 제시하였다 또한. 도면반입절차서 및 표준도면작성지침서, 그리고 GIS DB 구축지침서를 통하여 표준화된 데이터베이스 구축이 이루어지도록 하였다.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1609-1615
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• 2016

Breast cancer is one of among all cancers with increased incidence, high mortality rate, and high economic and social costs. The the most common type of cancer among females worldwide, breast cancer is actually the uncontrolled proliferation of cells which attain malignancy. Recently it has shown that breast cancer contributes 11% among all types of cancer diagnosed globally on an annual basis and it is one of the leading causes of death among women. The human epidermal growth factor receptor 2 (HER-2) is a receptor tyrosine-protein kinase erbB-2 normally involved in the proliferation and division of breast cells. In some abnormal cases the HER2 gene does not work correctly and makes too many copies of itself. HER2-positive (HER2+) breast cancers constitute an aggressive type of breast cancer and tend to grow faster and are more likely to spread. However, therapies that specifically target HER2, such as Herceptin$^{(R)}$ (traztuzumab), are very effective. HER2 targeted therapies, has significantly improved the therapeutic outcome for patients with HER2 positive breast cancer.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2008.04a
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• pp.77-86
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• 2008

It's been known that overexpression of the oncoprotein Her2 (eu/ErbB2), transmembrane receptor protein, occurs in human breast cancer. Her2-positive breast cancer patients who have Her2 overexpression show less therapeutic efficacy with enhanced metathesis and increased resistance to chemotherapy. So far, a humanized monoclonal antibody against Her2 protein called Herceptin is the only drug approved by Food and Drug Administration for treatment of Her2-overexpressing breast tumors. However, antibody therapy of Herceptin may not be ideal method for therapeutic intervention of Her2 protein expression. The therapeutic intervention of Her2 protein expression may be more efficiently achieved by inhibiting the expression of Her2 gene rather than by down-regulating the Her2 protein already overexpressed. Here, we found that the interaction of two proteins of ESX (an epithelial-restricted transcription factor) and DRIP130/CRSP130/Sur2 (a Ras-linked subunit of human mediator complexes) mediates the expression of Her2 gene. The association of ESX with Sur2 is mediated by a small hydrophobic face of 8-amino acid helix in ESX, suggesting that the ESX-Sur2 interaction can be a new novel target for Her2-positive cancer. The process to develop potent ESX-Sur2 interaction inhibitors targeting for Her2-positive cancer therapeutics will be discussed.

• Bulletin of the Korean Chemical Society
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• v.30 no.8
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• pp.1827-1831
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• 2009

Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDAMB- 231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.

• MALSORI
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• no.58
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• pp.19-34
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• 2006

The present study investigates the vowels of Bahasa Malaysia and Bahasa Indonesia in terms of the first two formant frequencies and provides a three-dimensional formant chart of vowels by plotting F1, F2, and the frequency of datapoints on 4 different scales: Hz, mel, bark, and the number of ERB. For this study, we recruited 30 male native speakers of Bahasa Malaysia and Bahasa Indonesia (15 each) which include 6 vowels (i, e, a, o, u, e) in various contexts. The three-dimensional formant chart showed geophysics of vowel space, such that mountain peaks stand in particular locations with a higher frequency of occurrence of datapoints. The geophysics of vowel space may shed lights on the perceptual structure of vowel space. The results also showed that vowels in utterance-final positions have a significantly higher F1 and a significantly lower F2 than those in utterance-medial or utterance-initial positions, which means that vowels in utterance-final positions are more back and lower in vowel space than those in utterance-medial or utterance-initial positions.

• Hwankyungkyoyuk
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• v.11 no.1
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• pp.237-250
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• 1998

'Self-esteem' is defined as 'the lived status of one's individual competence and personal worthiness in dealing with the challenges of Life over Time'. High self-esteem is associated with self-confidence, effectively coping, well-being, and responsibility and it is essential for the responsible choice and determination of environments. The purposes of this study were to develop a strategy to enhance the self-esteem and to verify the effects. A new strategy, 'Cognitive Restructuring Strategy' was based on the characteristics of self-esteem and the key idea of this was to eliminate negative thoughts and to reinforce affirmative thoughts. We developed the statement to embody this strategy and applied to the experimental group. According to the results, self-esteem for the control group(155) did not changed but that for the experimental group(158) was significantly enhanced. Continuously, environmental learning instructions of 3 units were carried out on two groups. By applying the t-test, achievement-test scores for the experimental group per unit were significantly higher than those of the control group as regards the four respective goals of EE. Therefore this strategy and statement are helpful in enhancing self-esteem and it was found that 'self-esteem' is a influential factor to form environmental responsible behaviors(ERB).

• Earthquakes and Structures
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• v.20 no.4
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• pp.389-403
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• 2021

Skewed bridges, being irregular structures with complicated dynamic behavior, are more susceptible to earthquake damage. Reliable seismic-resistant design of skewed bridges can be achieved by accurate determination of nonlinear seismic demands. However, the effect of geometric characteristics on the response modification factor (R-factor) is not accounted for in bridge design practices. This study attempts to investigate the effects of changes in the number of spans, skew angle and bearing stiffness on R-factor values and to assess the seismic fragility of skewed bridges. Results indicated that changes in the skew angle had no significant effect on R-factor values which were in consonance with code-prescribed R values. Also, unlike the increase in the number of spans that resulted in a decrease in the R-factor, the increase in bearing stiffness led to higher R-factor values. Findings of the fragility analysis implied that although the increase in the number of spans, as well as the increase in the skew angle, led to a higher failure probability, greater values of bearing stiffness reduced the collapse probability. For practicing design engineers, it is recommended that maximum demands on substructure elements to be calculated when the excitation angle is applied along the principal axes of skewed bridges.

• Herbal Formula Science
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• v.31 no.1
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• pp.41-51
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• 2023

Objectives : Raphani Semen (Raphanus sativus L.) is known for the various beneficial effects in Korean medicine. This study aimed to investigate the effect of Raphani Semen extract (RSE) against arachidonic acid (AA)+iron-induced oxidative stress in cells. Methods : Ingredients, their target information, oxidative stress liver injury-related proteins was obtained from various network pharmacology databases and software. A hypergeometric test and enrichment analysis were conducted to evaluate associations between protein targets of RSE. The cell viability was assessed by MTT assay, and immunoblot analysis was used to confirm the molecular mechanisms. Results : A compound-target network of RSE was constructed, which consisted of 336 edges between 18 ingredients and 123 protein targets. PI3K-Akt signaling pathway, ErbB signaling pathway, HIF-1 signaling pathway, PPAR signaling pathway, and AMPK signaling pathway was significantly associated with protein targets of RSE. RSE protected HepG2 cells against AA+iron-induced oxidative stress as mediated with AMPK signaling. Conclusion : RSE was found to protect the cells against oxidative stress via the AMPK signaling pathway.