• 환경사랑
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• s.66
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• pp.11-11
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• 2012

• Journal of Biomedical Engineering Research
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• v.37 no.1
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• pp.46-52
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• 2016

Nanoparticles have been studied as therapeutic and imaging agents for the early detection and cure of cancer, Cancer Theranostics. Nanoparticles were considered to effectively target cancer cells due to Enhanced Permeability and Retention (EPR) effect and most nanoparticles have been evaluated by using spherical shapes. However, the problem that the EPR effect is not so effective for human cancer therapy was recently brought up. Therefore, in this study, we suggest novel discoidal nanoparticles to overcome this problem, focusing on their manufacturing process and quality control. Herein, we demonstrate the improved manufacturing method of discoidal nanoparticles and their potential to apply to MCF 7, human breast cancer treatment.

• Journal of the Korea Computer Industry Society
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• v.6 no.2
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• pp.357-364
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• 2005

The prefabricated type used generally in Korea to join cable runs on new installations or to repair broken Cable runs on existing installations, because installation is very simple and save time. This type is a permanent, shielded and submersible cable joint for direct burial or vault application. It confirms to the requirements of IEEE std. 404-1993 by factory testing, but many problems of insulated cable systems are caused by internal defects of the joint part which have to be mounted ensile. Faults arise from impurities or voids. A suitable solution for a monitoring of cable joints during the after-laying test and service is partial discharge detection. Specimen obtained 1mm thickness from the insulation of real power cable and cable joint. <중략>The partial discharges are measured to determine their time dependence for 60 minutes and the influence of applied electrical stress under 30kV. $\Phi-q-n$ properties were measured using detection impedance, high pass filter and computerized data acquisition system. Statistic Value like maximum charge, repetition rate, average charge, etc. are calculated. It is possible to quantitative analysis of $\Phi-q-n$ properties from this statistic value and pattern analysis.

• Journal of the Korea Computer Industry Society
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• v.6 no.2
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• pp.279-286
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• 2005

The prefabricated type used generally in Korea to join cable runs on new installations or to repair broken Cable runs on existing installations, because installation is very simple and save time. This type is a permanent, shielded and submersible cable joint for direct burial or vault application. It confirms to the requirements of IEEE std. 404-1993 by factory testing, but many problems of insulated cable systems are caused by internal defects of the joint part which have to be mounted ensile. Faults arise from impurities or voids. A suitable solution for a monitoring of cable joints during the after-laying test and service is partial discharge detection. <중략> $\Phi-q-n$ properties were measured using detection impedance, high pass filter and computerized data acquisition system. Statistic Value like maximum charge, repetition rate, average charge, etc. are calculated. It is possible to quantitative analysis of $\Phi-q-n$ properties from this statistic value and pattern analysis.

• BMB Reports
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• v.30 no.6
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• pp.397-402
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• 1997

A thiol-specific spin label was attached to cysteine-102 of yeast cytochrome c and electron paramagnetic resonance (EPR) spectra were measured as a function of added cytochrome c oxidase concentration. The intensity decreased due to line broadening as cytochrome c formed a complex with cytochrome c oxidase and reached a minimum when the ratio of cytochrome c to cytochrome c oxidase became one. Replacement of either Lys-72 or Lys-87 of cytochrome c by Glu did not result in a significant change in binding affinity. Interestingly the K72E mutant, unlike K87E, had a much lower rate of electron transfer than the wild type. These results indicate that many positively charged residues as a group participate in complex formation but Lys-72 might be important for cytochrome c to be locked in an orientation for an efficient electron transfer. A stoichiometry of 1 was also confirmed by optical absorption of the cytochrome c-cytochrome c oxidase complex which had been run through a gel chromatography cloumn to remove unbound cytochrome c. The EPR spectrum of this 1:1 complex, however, was a mixture of two components. This explains a biphasic kinetics for a single binding site on cytochrome c oxidase without invoking conformational transition.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.28 no.8
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• pp.501-507
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• 2015

In this paper, EPR (ethylene propylene rubber) insulation material was accelerated degradation test at $121^{\circ}C$, $136^{\circ}C$, $151^{\circ}C$, and experiment the typical EAB (elongation at break) at mechanical characteristics analysis. It is shown that the failure-time at the point of 50% of the initial value of Elongation rate to obtain the activation energy. The failure-time was shown each 5,219 hr, 3,165 hr, and 668 hr at three temperatures. In order to derive the activation energy, Arrhenius methodology was applied. Also, we got the Arrhenius plot from three accelerated temperatures. The activation energy values got 0.98 eV from EAB test. The experimental data were evaluated for estimating the probability density, and the suitable distribution by using statistical program MINITAB. It is shown that EAB data by the acceleration thermal degradation is most suitable for the Weibull distribution.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.49-54
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• 2000

Cr-Mo-V steel is widely used as a material for the turbine structural component in fossil power plants. It is well known that this material shows the various material degradation phenomenons such as temper embrittlement, carbide coarsening. and softening etc. or ins to the severe operation conditions as high temperature and high pressure. These deteriorative factors cause tile change of mechanical properties as reduction of fracture toughness. Therefor it is necessary to evaluate tile extent of degradation damage for Cr-Mo-V steel in life assessment of turbine structural components. In this paper. the electrochemical potentiokinetic reactivation(EPR) test in $50wt%-Ca(NO_3)_2$ solution is performed to develop the newly technique for degradation damage evaluation of Cr-Mo-V steel. The results obtained from the EPR test are compared with those in small punch(SP) tests recommended by semi-nondestructive testing method using miniaturized specimen. The evaluation parameters used in EPR test are tile reactivation current density$(I_R)$ and charge$(Q_{RC})$ reactivation rate$(I_R/I_{Crit},\;Q_R/Q_{Crit})$. The results suggest that $I_R/I_{Crit}$ in these parameters shows a good correlation with SP test results.

• Ocean and Polar Research
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• v.40 no.3
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• pp.115-125
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• 2018

Production from copepodite IV to adult of two euchaetid species Euchaeta plana and Paraeuchaeta russelli was measured at the southeastern sea of Korea from April to November, 2014. The mean density was $2.0ind\;m^{-3}$ for E. plana and $4.1ind\;m^{-3}$ for P. russelli, with the high contribution of copepodite V to total density. The densities of total individuals, adult females and eggs were highest in November for both species. The mean egg production rate (EPR) was $1.7eggs\;female^{-1}\;d^{-1}$ for E. plana and $3.1eggs\;female^{-1}\;d^{-1}$ for P. russelli. Both of them showed the highest EPR in September but zero EPR in summer. The mean weight-specific EPR was $0.038d^{-1}$ for E. plana and $0.079d^{-1}$ for P. russelli. The mean total production rates of E. plana and P. russelli were $5.3{\mu}g\;C\;m^{-3}\;d^{-1}$ and $17.8{\mu}g\;C\;m^{-3}\;d^{-1}$, respectively, with the largest production in November. The mean Production/Biomass ratio was $0.06d^{-1}$ for E. plana and $0.07d^{-1}$ for P. russelli, with its peak in September for both. The total production of E. plana and P. russelli was positively correlated with the density of a copepod Oncaea venusta, rather than chl-a concentration, indicating that the two copepods might be carnivores. This study evaluates the contribution of euchaetids to the copepod community in the southeastern sea of Korea.

• Analytical Science and Technology
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• v.8 no.4
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• pp.869-876
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• 1995

The active sites of the nickel and iron-containing enzyme, carbon monoxide dehydrogenase (CODH) from clostridium thermoaceticum were investigated using Electron Paramagnetic Resonance (EPR) technique. CODH exhibits several spectral features called NiFeC, $g_{ave}=1.82$, $g_{ave}=1.86$. FCII signals which are originated from different clusters in this enzyme. CODH is know to catalyze two different kinds of reactions - acetyl-CoA synthesis and CO oxidation. The acetyl-CoA synthesis activity can be followed by monitoring CO/acetyl-CoA exchange. The addition of 1,10-phenanthroline (phen) to CODH selectively destroyed the CO/acetyl-CoA exchange activity and eliminated the NiFeC signal completely. CO oxidation activity and other EPR signals were unaffected. Such behavior demonstrates that CODH has two distinct active sites and that the NiFe complex is only responsible for the CO/acctyl-CoA exchange activity. Phen caused the removal of only 30% of Ni in the NiFe complex ($0.3Ni/{\alpha}{\beta}$) as shown by the quantitative metal analysis. The phen-treated CODH could be reactivated fully by incubation In $Ni^{2+}$ solution. Radioactive $^{63}Ni^{2+}$ was used to quantitate the amount of the $Ni^{2+}$ incorporated into phen-treated enzyme and showed that the amount was the same as the removed by the phen treatment. i.e. $0.3Ni/{\alpha}{\beta}$. This indicates that only 30% of NiFe complexes are labile and responsible for the CO/acctyl-CoA exchange activity, the other 70% are non-labile and have no exchange activity. This is the first clear evidence that the NiFe complex is heterogencous and labile and non-labile Ni sites arc interacting differently with substrates and chelating agents like phen.

• Macromolecular Research
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• v.16 no.1
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• pp.15-20
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• 2008

The enhanced permeability and retention (EPR) effect is used extensively for the passive targeting of many macromolecular drugs for tumors. Indeed, the EPR concept has been a gold standard in polymeric anticancer drug delivery systems. This study investigated the tumoral distribution of self-assembled nanoparticles based on the EPR effect using fluorescein and radio-labeled nanoparticles. Self-assembled nanoparticles were prepared from amphiphilic chitosan derivatives, and their tissue distribution was examined in tumor-bearing mice. The size of the nanoparticles was controlled to be 330 run, which is a size suited for opening between the defective endothelial cells in tumors. The long-circulating polymer nanoparticles were allowed to gradually accumulate in the tumors for 11 days. The amount of nanoparticles accumulated in the tumors was remarkably augmented from 3.4%ID/g tissue at 1 day to 25.9%ID/g tissue at 11 days after i.v. administration. The self-assembled nanoparticles were sustained at a high level throughout the 14 day experimental period, indicating their long systemic retention in the blood circulation. The ${\gamma}$-images provided clear evidence of selective tumor localization of the $^{131}I$-labeled nanoparticles. Confocal microscopy revealed the fluorescein-labeled nanoparticles to be preferentially localized in the perivascular regions, suggesting their extravasation to the tumors through the hyperpermeable angiogenic tumor vasculature. This highly selective tumoral accumulation of nanoparticles was attributed to the leakiness of the blood vessels in the tumors and their long residence time in the blood circulation.