• Journal of Microbiology
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• v.44 no.1
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• pp.121-125
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• 2006

Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about $10\%$ of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase).

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.4
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• pp.252-263
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• 2001

Nematode infections compromise human health and reduce agricultural productivtiy. Experiments that exploit the powerful molecular genetics of the free-living nematode Caenorhabdl - elegans have contributed to our understanding of how the major classes of anthelmintic nema-tocides kill worms and how worms might evolve resistance to these drugs In C. elegans, as in parasites, benzimidixoles interfere with microtubule polyumerization the imidazothiazoles/tetra-hydropyrimidines activate nicotinic acetylcholine receptors, and the macrocyclic la ctones activate qlutamate-gate chloride chanels. Mutant alleles of genes that encode drug targes often confer resistance in C. elegans. Preliminary evidence suggests that alleles of homologous genes in parasites will, in many cases, also play a role in resistance. Thus information acquired from C. elegans can be usefully applied to understand the mechanisms of drug sensitivity and the genetics of resis-tance in parasites.

• BMB Reports
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• v.39 no.3
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• pp.263-269
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• 2006

In the genome of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, open reading frame 39 (Ha39) is the only gene predicted to encode an RNA recognition protein. Computer analysis revealed that Ha39 homologues were found in 15 NPVs, but not in GVs. Its transcripts were detected from 3 through 72 hours post infection (h p.i.) using RT-PCR and Northern blot analysis. The protein was detected in infected-cell lysates from 6 h p.i. Western blot assay of ODV and BV preparations revealed that Ha39 encodes a structural protein associated with BVs. Additionally, immunofluorescence microscopy demonstrated that the protein was present within cytoplasm in virus-infected cells, but not in the nuclear region.

• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.666-669
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• 2010

A target bacterial strain of interest for use in Red-based recombineering may already encode resistance to antibiotic markers used with current Red recombination tools, such that the resistance cannot be removed. Such cases include those where markers are needed to maintain an unstable genetic element co-resident in the strain or those where the genetic source of resistance is not known. We report the availability of PCR templates with FRT-flanked mutagenesis cassettes and plasmids encoding Red recombination functions that contain marker combinations not currently available on widely disseminated lambda Red molecular reagents. The functionality of these convenient alternative tools is demonstrated.

• Journal of Information Processing Systems
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• v.4 no.2
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• pp.67-76
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• 2008

This research proposes a new strategy where documents are encoded into string vectors and modified version of k means algorithm to be adaptable to string vectors for text clustering. Traditionally, when k means algorithm is used for pattern classification, raw data should be encoded into numerical vectors. This encoding may be difficult, depending on a given application area of pattern classification. For example, in text clustering, encoding full texts given as raw data into numerical vectors leads to two main problems: huge dimensionality and sparse distribution. In this research, we encode full texts into string vectors, and modify the k means algorithm adaptable to string vectors for text clustering.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.6B
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• pp.1174-1182
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• 1999

Recently the shape information of the visual object in the scene is more important, as the completion of the MPEG-4 standard and the progress of the MPEG-7 standard. This paper represents the study of effective coding method of vertices that are used in the polygonal approximation to represent the feature of visual object. In the proposed method, we make the centers of gravity of triangles that are made using the vertices of polygonal approximation and encode them sequentially We can get a coding gain because the centers of the gravity of triangles have narrower dynamic ranges.

• Investigative Magnetic Resonance Imaging
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• v.1 no.1
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• pp.79-85
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• 1997

Fast spin echo imaging utilizes multiple spin echoes to encode multiple k-space lines instead of multiple $T_2-weighted$ images. As results, intensities in k-space data are varying according to T2 decay, which generates Gibb's artifact in the reconstructed image. The echo time for e encoding dc block determines contrast, as is specified by the effective echo time, however, all location of other echoes to different k-space frequency blocks in fast spin echo imaging is not f fully investigated. In this study, symmetric arrangement of multiple echoes in k-space is investigated to reduce Gibb's artifact. Design of filters based on the measurement of multiple e echo intensities is also proposed in two stage manner, i.e., equalization and filtering. From s simulation and experiment, it was observed that Gibb's phenomena were substantially reduced b by the proposed methods.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.7
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• pp.120-127
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• 2003

A novel three degrees of freedom sensing method utilizing binary grid pattern image and vision camera is presented. The binary grid pattern image is designed by Pseudo-Random Binary Arrays and referenced to encode in-plane position of a moving stage of the contact-free planar actuator. First, the yaw motion of the stage is detected using fast image processing and then the other planar positions, x and y, are decoded with a sequence of images. This method can be applied to the system that needs feedback of in-plane position, with advantages of a good accuracy and high resolution comparable with the encoder, a relatively compact structure, no friction, and a low cost. In this paper, all the procedures of the above sensing mechanism are described in detail, including simulation and experiment results.

• The Plant Pathology Journal
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• v.36 no.5
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• pp.491-496
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• 2020

An understanding of the contribution of secondary metabolites (SMs) to the antagonistic and biocontrol activities of bacterial biocontrol agents serves to improve biocontrol potential of the strain. In this study, to evaluate the contribution of each SM produced by Pseudomonas fluorescens NBC275 (Pf275) to its antifungal and biocontrol activity, we combined in silico analysis of the genome with our previous study of transposon (Tn) mutants. Thirteen Tn mutants, which belonged to 6 biosynthetic gene clusters (BGCs) of a total 14 BGCs predicted by the antiSMASH tool were identified by the reduction of antifungal activity. The biocontrol performance of Pf275 was significantly dependent on 2,4-diacetylphloroglucinol and pyoverdine. The clusters that encode for arylpolyene and an unidentified small linear lipopeptide influenced antifungal and biocontrol activities. To our knowledge, our study identified the contribution of SMs, such as a small linear lipopeptide and arylpolyene, to biocontrol efficacy for the first time.

• Journal of Biomedical Engineering Research
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• v.13 no.4
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• pp.323-330
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• 1992

A Study of Image Compression Algorithm using DCT This paper describes the system that implement a JPEG(Joint Photographic Experts Group) algorithm based on DCT(Discrete Cosine Transform) uslng CCD kameva, Image Grabber, and IBM PC. After cosine transforms the acquisited image, this algorithm quantize and entropy encode the coefficients by JPEG code table. The coefficients are reconstructed by the Huffman decoding, dequantized procedure, and Inverse cosine transform. The results obtained from the impleulented system are as follows. (1) For effcient storage and easy implementation, this system save Image as a PCX formal (2) Thls system get 7:1 compression ratio(3.8 RMSE value) without large distortion. (3) With a low pass filtering, thls system eliminate high frequency components and get 20% enhanced compression ratio. (4) Thls system enhance the reconstructed Image using histogram modeling.