• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.40-44
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• 1995

This study was aimed to mutant induction in Gentiana scabra BUNGE through the tissue culture techniques and comparison of gentiopicroside content in each mutant plants derived from tissue culture. In order to induce the mutants of Gentiam scabra, seed, apical and lateral bud were soaked in $NaN_3$, EMS and MNU solutions and cultured on MS basal medium containing 0.5mg/l NAA, BA $NaN_3$, MNU-treated buds survived about 50% but not survived in EMS treatment. Seed germination rate was extremely low in EMS and MNU treatments but various types of mutant plantlets were obtained by those treatments. 63% of elongated type and 37% of dwarf type were obtained from tissue culture treated by various mutagen. Gentiopicroside contents of regenerated plantlets was analyzed. Root of dwarf type contained more gentiopicroside(1.383%) than that of elongation type (0.083%).

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.83-83
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• 2003

돼지 난자의 유리화 동결 처리 방법 중 난자를 담는 용기(loading vessel)의 재료로 최근에 알려진 것으로 open-pulled straws(OPS)[Vajta등, Mol Reprod Dev, 51:53-58, (1998)], electron microscope grids(EMG) [Martino등,Biol Reprod, 54:1059-1069, (1996)〕, nylon loop system(NLS) [Lane등, Fertil Steril,72: 1073-1078, (1999)] 등이 보고되고 있다. OPS는 1/4cc straws를 열을 가하여 길게 뽑아 내벽을 얇게 함으로써 filing된 난자나 수정란이 액체 질소와 접촉했을 때 유리화가 신속하게 되도록 하는 방법으로 돼지에서는 별로 보고된 것이 없다. EMG는 열전도가 예민한 전자현미경용 copper grid를 이용한 방법으로 최근 국내 기술진의 연구성적을 포함한 몇몇 학자들에 의하여 보고되었고 NLS는 0.5mm직경의 nylon loop를 이용하여 급속 동결한 성적이 보고되었으나, 돼지 난자에 응용 된 것은 없다. 따라서 이와 같은 동결 재료는 사람과 반추류, mouse외에 돼지 난자에 대하여는 전혀 시도되지 않았지만 유리화 동결기술에서 가장 중요한 실험으로 생각된다. 성공적인 유리화 동결을 위해서는 수정란이 냉각의 전도성이 빠르고, 작은 용액을 수정란과 같이 filling해야 하며 모든 동작이 신속 간편해야 하며 융해 방법도 초급속도의 융해가 요구되므로 이에 부합되어야 한다. 연구 목적은 돼지 난자를 유리화 동결/융해 시 동결 재료-straw/glass, copper grid, nylon 3가지에 대한 제작 방법, 난자 loading, 동결 처리, 보관 방법, 융해 방법 등을 난자의 회수, 수정 후 생존율을 비교 조사하여 가장 우수한 방법을 선택할 목적이었다. 수행 내용은 3가지의 재료의 sample을 제작하고 소독한 다음 준비된 돼지 COCs을 40시간동안 IVM한 후 난자를 5~l5개 정도로 선정 하여 준비된 VS 용액에 평형처리 하였다. 각 재료의 용기에 loading 한 후 동결/보관하였고, 융해는 역순으로 평형하여 maturation 배지에 3~4시간 배양한 다음 경검하고 IVF한 후 NCSU-23 배지에 담아 IVC 배양하면서 cell cleavage상태를 확인하였다.

• Journal of Advanced Navigation Technology
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• v.21 no.6
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• pp.614-619
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• 2017

In this paper, we optimize the wireless power transmission (WPT) coil, and then compare the EM simulation and measurement using magnetic coupling at 6.78 MHz. As transmission efficiency is affected by various factors such as the shape of the system, the size of the coils, the coil structure is proposed to consist of a helical resonant for transmission and a spiral resonant for reception. The size of the coil and the distance between the coils are determined to minimize the volume problem, and the shape of the coil are confirmed by EM simulation. A WPT system is designed with 860mm diameter top plate and cylindrical structure of column spaced 600mm apart, and the characteristics are simulated and measured. The simulation shows that ${\mid}S_{21}{\mid}$ is -0.53 dB with the efficiency of 88%, and the measurement result is that ${\mid}S_{21}{\mid}$ is -0.71 dB with the efficiency of 85%.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.05a
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• pp.63-67
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• 2009

In RFID system, Tag's Identification data is processed in host computer by application program. Example of application program is parking administration program, library state program etc.. Tag's ID that is recognized in reader is inputted to the Host computer. Application program of computer searches data in DB of computer such as Tag ID. After finding the same ID, host computer send to control command to driver H/W in accordance with application purpose. But, It need to confirm in long distance whether achievement process is acted normally. There will be the 2 methods, when we monitors the process in long distance. One is wired monitoring system, another is wireless monitoring system. Among wire method, internet communication network is useful. RFID system manufacture first in this treatise. RFID system Embody using EM4095 chip that is doing 125KHz by carrier wave. Tag's characteristic ID is sent in remote place through module that use W3100A chip. This system Manufacture, and data send-receive confirmed using simple application program. Reception confirm by result, and pictures show by whole system and each part. And a control program explained of each part.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.18 no.6A
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• pp.51-61
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• 2008

This paper suggests a new way to implement high speed AES on Intel Core2 processors and AMD Athlon64 processors, which are used all over the world today. First, Core2 Processors of EM64T architecture's memory-access-instruction processing efficiency are lower than calculus-instruction processing efficiency. So, previous AES implementation techniques, which had a high rate of memory-access-instruction, could cause memory-bottleneck. To improve this problem we present the partial round key techniques that reduce the rate of memory-access-instruction. The result in Intel Core2Duo 3.0 Ghz Processors show 185 cycles/block and 2.0 Gbps's throughputs in ECB mode. This is 35 cycles/block faster than bernstein software, which is known for being the fastest way. On the other side, in AMD64 processors of AMD64 architecture, by removing bottlenecks that occur in decoding processing we could improve the speed, with the result that the Athlon64 processor reached 170 cycles/block. The result that we present is the same performance of Matsui's unpublished software.

• Korean journal of applied entomology
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• v.38 no.1
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• pp.53-57
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• 1999

Simple diagnostic kits for monitoring insecticide susceptibility of beet armyworm, Spodoptera exigua (Hiibner) were developed and applied to the field populations. The operation of the kits was based on the correlations between enzyme activities of esterase (EST) and acetylcholinesterase (AChE) and the insecticide susceptibilities. Four different kinds of diagnostic kits (ED, EM, AD, and AM) were designed and classified by diagnostic enzymes (E for esterases and A for acetylcholinesterase) and inhibitors (D for dichlorvos and M for monocrotophos). Diagnostic inhibitor concentrations were 1 mM for ED, 10 mM for EM, 100 mM for AD, and 100 mM for AM. Resistant larvae which were not inhibited by the diagnostic amounts of insecticides developed positive staining (red color), but susceptible~ s howed negative (no color). An insect was used for both EST and AChE diagnostic kits, but different in their samples: hemolymph for EST and the head for AChE. These four diagnostic kits were applied to 1 1 different populations which showed variations of insecticide susceptibilities. Four kits were different in the capability discriminating the insecticide susceptibilites according to insecticides: ED to bifenthrin, AD to methomyl, and ED and AM to chlorpyrifos-methyl. These diagnostic devices can be used for insecticide-resistance management program for this insect pest. It also provide a technical guide to insect pest management for farmers, directors, and researchers.

• KSBB Journal
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• v.11 no.1
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• pp.8-14
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• 1996

Bacillus subtillis strains with transition state regulator mutations and a spore mutation were developed for the overexpression of apsE and for the enhancement of expression level. Among the many regulator genes, degU and hpr were chosen as a representative positive and negative regulator for the aprE, respectively. Spo II G was used for the construction of asporogeneous strains. All the mutants were constructed from two protease-deleted strain DB104 and the apsE gene was transformed with an integration vector pMK101. DB104(deg$U^h$(32) $his^+$)::pMK101(Cm) and DB104($\Delta$her(Em))::pMKl01(Cm) show 7-fold and about 2-fold increase in aprE expression level, respectively. But the effect of transition state regulator mutation on the aprE expression was diminished when the integrated aprE gene was amplified by the high concentration of chloramphenicol, i. e. 30 $\mu\textrm{g}$/ml. DB104($\Delta$spoIIG(Pm) degUh(32) his+)::pMK101(Cm) and DB104($\Delta$spoIIG(Pm) $\Delta$hpr(Em))::pMK101 double mutant show 10-fold and 3-fold increase in aprE expression level, respectively. The results suggest that sporulation mutation and transition state regulator mutation have independent and additive effect on the aprE expression, and the same gene dosage effect on the transition state regulator mutation was also identified.

• Journal of the Korean Society for Railway
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• v.18 no.1
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• pp.63-70
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• 2015

Track irregularities have a strong influence on the safety and the ride comfort of trains as well as track maintenance work. A statistical analysis was conducted for track irregularities data measured in Kyoungbu high speed line using a track recording car(EM120). First, the characteristics of EM120, which adopted versine measurement system, were investigated and the transfer function of EM120 was examined. The distributions of the surface and the alignment which have short wavelength were considerably high and the distribution decreased as wavelength increases. A distribution analysis of the wavelength was carried out according to EN standards and most of the track irregularities were in a D1 wavelength range. The distributions of the amplitude of track irregularities were also investigated and the results show that the distributions of track irregularities decrease rapidly as amplitude increases. These results may be helpful in making track maintenance plans and revising track irregularities criteria.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.323-331
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• 1996

Predetermination of sex in mammalian species has many aspects of application including the prenatal diagnoses of genetic disorders in humans and sex-selected breeding programs in the animal industry. Embryos sexing can be carried out using the polymerase chain reaction (PCR) to amplify specific sequences present in the sex chromosomes, or by fluorescent in situ hybridization (FISH) of specific probes to the X and Y chromosomes. A 3.3 kb porcine male-specific DNA fragment (pEM39) was cloned previously in our laboratory. In this study, FISH and PCR methods were employed to examine if the pEM39 can be used a sex-specific DNA probes Porcine ovaries were obtained from a local slaughter house and oocytes collected. All oocytes were subjected to in vitro maturation followed by 1n vitro fertilization. Parthenogenetically activated embryos were served as a negative control. Embryonic samples were collected at the 2-cell stages and PCR was performed to analyze DNA. Among 10 embryos examined, four embryos were identified as males and six were females. The cloned male-specific DNA fragment showed male-specificity for the cells in the liver tissue and the porcine early embryos by FISH. It was also demonstrated that the cloned male-specific DNA is localized on the hetero chromatic region of the long arm in the Y chrom-osome (Yq) as shown by the FISH and karyotyping. The results suggest that the cloned male-specific DNA fragment may be useful for predetermination of sex with a few embryonic cells. The porcine male-specific sequence can be a reliable index for embryo sexing by PCR.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.22 no.12
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• pp.1180-1183
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• 2011

In this paper, we present a design of AEM radome with frequency selective surface using unit-cell EM simulation method and verified the performance of AEM radome by the radiation pattern measurements of $16{\times}16$ array-antenna. Measured results under near-field condition showed a good agreement with the simulation and far-field measured ones. And there were no radiation pattern variation between antenna only and antenna with AEM radome. From these results, we verified that AEM radome with frequency selective surface can be a solution to reduce the RCS of conventional mast and radar.