• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.739-747
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• 2018

The low solubility and high-cost recovery of Propionibacterium acnes polyunsaturated fatty acid isomerase (PAI) are key problems in the bioproduction of high value-added conjugated linoleic acid (CLA). To improve the solubility of recombinant PAI, six chaperone proteins were coexpressed with PAI. Introduction of GroELS proteins dramatically improved the PAI solubility from 29% to 97%, with increased activity by 57.8%. Combined expression of DnaKJ-GrpE and GroELS proteins increased the activity by 11.9%. In contrast, coexpression of DnaKJ-GrpE proteins significantly reduced the activity by 57.4%. Plasmids pTf16 harboring the tig gene and pG-Tf2 containing the tig and groEL-groES genes had no visible impact on PAI expression. The lytic protein E was then introduced into the recombinant Escherichia coli to develop a cell autolysis system. A 35% activity of total intracellular PAI was released from the cytoplasm by suspending the lysed cells in distilled water. The PAI recovery was further improved to 81% by optimizing the release conditions. The lipase from Rhizopus oryzae was also expressed in E. coli, with an extracellular activity of 110.9 U/ml. By using the free PAI and lipase as catalysts, a joint system was established for producing CLA from sunflower oil. Under the optimized conditions, the maximum titer of t-10,c-12-CLA reached 9.4 g/l. This work provides an effective and low-cost strategy to improve the solubility and recovery of the recombinant intracellular PAI for further large-scale production of CLA.

• Polymer(Korea)
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• v.28 no.6
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• pp.524-530
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• 2004

Polystyrene particles (PS) with poly(ethylene glycol) units on surface were formed by an emulsifier-free emulsion polymerization using styrene, poly(ethylene glycol) methacrylate (PEG-MMA) or poly(ethylene glycol) dimethacrylate (PEG-diMMA) at pH 7, and followed by freeze-drying to give the corresponding powders. The structures of PS particles were confirmed by FT-IR spectroscopy, and the particle size and distribution the PS particle were observed by scanning electron microscopy and particle analyzer. Monodisperse polymer particles were obtained at a concentration of PEG-MMA 2∼5 mol% or PEG-diMMA 1 mol% relative to styrene. The highest zeta potential of polymer surface was measured to be 183 mV at a polymer of PEG-MMA 5 mol%, which was measured in dielectric medium by means of ELS-8000 dynamic light scattering.

• Explosives and Blasting
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• v.37 no.3
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• pp.1-12
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• 2019

The analysis methods of blast analysis models are classified into direct analysis and indirect analysis, and the latter is divided into semi-empirical and numerical analysis methods. In order to evaluate the applicability of the ELS blast analysis program, which is a program for analyzing the semi-empirical models, this study selected a simplified analytical model and examined the blast load characteristics of free-air burst explosion and surface burst explosion by using AT-Blast, RC-Blast, and Kinney and Graham's empirical equations, which are the semi-empirical analysis programs. As a result of analyzing the explosion pressure for the scaled distance and the incidence angle for the simplified analytical model, an appropriate analysis can be performed when the range of the scaled distance in the free-air burst explosion analysis was 0.3~0.461 and when the range of the scaled distance in the surface burst explosion analysis was 0.378~0.581. In terms of the incidence angle, the results analyzed within $45^{\circ}$ were considered to be appropriate.

• Journal of Advanced Marine Engineering and Technology
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• v.25 no.5
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• pp.1140-1147
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• 2001

This paper describes the effect of various molding pressure for Mode I. Mode II interlaminar fracture toughness of carbon fiber reinforced plastic composites by using double cantilever beam(DCB), end notched flexure(ENF) and end loaded split(ELS) Specimen. The value of $G_{IC}$, $G_{IIC}$ as a function of various molding pressure is almost same at 307, 431, 585 kPa, however it shows highest value under 307kPa molding pressure, The SEM photographs show good fiber distribution and interfacial bonding of composites when the molding pressure is the 307kPa.

• Korean Journal of Computational Design and Engineering
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• v.8 no.1
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• pp.10-18
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• 2003

FEM (Finite Element Method) is a fundamental numerical analysis technique in wide spread use in engineering application. As the solving time occupies small portion of entire FEM analysis time because of development of hardware, the relative lime to the whole analysis time to make mesh mod-els is growing. In particular, in the case of stiffeners such as features attached to plate in ship structure, the line constraints are imposed on mesh model together with other constraints such as holes. To auto-matically generate two dimensional quadrilateral mesh with the line constraints, an algorithm is pro-posed based on the constrained Delaunay triangulation and Q-Morph algorithm in which the line constraints are not considered. The performance of the proposed algorithm is evaluated. And some numerical results of our proposed algorithm ate presented.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.24 no.12
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• pp.214-220
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• 2010

This paper presents the results of a study on an electrodeless high intensity discharge (HID) lamp system that is powered by circularly polarized microwaves (CPMs). The technique to generate CPMs enables an electrodeless high intensity discharge lamp to be turned on without the retation of the bulb but conventional electrodeless high intensity discharge lamps use rotating bulbs in order to prevent a puncture in a hot spots that are formed by the linearly polarized microwaves in the circular cavity. The technique to generate CPMs is described and the salient features of the lamp characteristics are presented.

• Bulletin of the Korean Mathematical Society
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• v.50 no.6
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• pp.2035-2051
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• 2013

In this paper, we study numerical schemes for solving multi-dimensional option pricing problem. We compare the direct solving method and the Operator Splitting Method(OSM) by using finite difference approximations. By varying parameters of the Black-Scholes equations for the maximum on the call option problem, we observed that there is no significant difference between the two methods on the convergence criterion except a huge difference in computation cost. Therefore, the two methods are compatible in practice and one can improve the time efficiency by combining the OSM with parallel computation technique. We show numerical examples including the Equity-Linked Security(ELS) pricing based on either two assets or three assets by using the OSM with the Monte-Carlo Simulation as the benchmark.

• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1887-1892
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• 2008

Novel poly(aspartic acid) derivatives conjugated with L-lysine moieties and their amphiphilic analogs were synthesized and characterized. The chemical structures of these polymers were confirmed using FT-IR and $^1HNMR$ spectroscopy. The physicochemical properties of amphiphilic copolymers were characterized using an electrophonetic light scattering spectrophotometer (ELS) and transmission electron microscopy (TEM). These results indicated a stable nanoparticle formation within aqueous media. These polymers have potential applications in the pharmaceutical and cosmetic fields as delivery vehicles for bioactive molecules.

• Bulletin of the Korean Chemical Society
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• v.30 no.12
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• pp.3025-3030
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• 2009

Novel poly(aspartic acid) derivatives conjugated with $\gamma$-amino butyric acid, GABA, moieties, and their amphiphilic analogs were synthesized and characterized. The chemical structures of these polymers were confirmed by FT-IR and $^1H$ NMR spectroscopy. Their physicochemical properties in aqueous media were characterized by electrophonetic light scattering spectrophotometry (ELS), acid-base titration, and UV-spectroscopy. In addition, the in vitro cell activity of the GABA-conjugated polymer was examined. These results indicated that GABA-conjugated poly(aspartic acid) derivatives showed cell-growth activity and nanoparticle formation of a suitable size within aqueous media. These polymers have potential application in the cosmetic and pharmaceutical fields.

• Proceeding of KASS Symposium
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• 2006.05a
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• pp.170-174
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• 2006

This paper presents a new nonlinear analysis algorithm which uses the equivalent nodal load for the element stiffness. The equivalent nodal load represents the influence of the stiffness change such as the addition of elements, the deletion of elements, and/or the partial change of element stiffness. The nonlinear analysis of structures using the equivalent load improves the efficiency very much because the inverse of the structural stiffness matrix, which needs a large amount of computation to calculate, is reused in each loading step. In this paper, the concept of nonlinear analysis using the equivalent load for the element stiffness is described and some numerical examples are provided to verify it.