• 동력기계공학회지
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• 제20권6호
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• pp.93-98
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• 2016

Environmental problems are issued throughout all over the world and which are needed the strength management. In case of the diesel cars are also being developing and studying continuously about various after-treatments device such as EGR, LNT, SCR, DPF and DOC etc. which are used for decreasing $NO_X$ and PM. The air temperature goes up to $39^{\circ}C$ in summer and goes down to $-20^{\circ}C$ in winter because of the location. These changing of the temperature can effect to the engine and harmful exhaust gas discharged and it seems to make the increase - decrease different. The result of the evaluate while changing between the test-mode and the air temperature, which expresses that WLTC-mode is 2.2 times and FTP_75 mode is 4.1~6 times increase to the comparison NEDC-mode of the current regulation. The exhaust characteristic of $NO_X$ by the changing temperature increases in the low temperature and 4.3 times in $14^{\circ}C$ and 21.3 times in $-7^{\circ}C$ with maximum when it compares to $23^{\circ}C$. The fuel efficiency of the different weight car and engine with same data is about 5.7 % in maximum.

• Journal of Korean Neurosurgical Society
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• 제65권5호
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• pp.697-709
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• 2022

Objective : The present study aimed to identify the function of ischemic stroke (IS) patients' peripheral blood and its role in IS, explore the pathogenesis, and provide direction for clinical research progress by comprehensive bioinformatics analysis. Methods : Two datasets, including GSE58294 and GSE22255, were downloaded from Gene Expression Omnibus database. GEO2R was utilized to obtain differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs were performed using the database annotation, visualization and integrated discovery database. The protein-protein interaction (PPI) network of DEGs was constructed by search tool of searching interactive gene and visualized by Cytoscape software, and then the Hub gene was identified by degree analysis. The microRNA (miRNA) and miRNA target genes closely related to the onset of stroke were obtained through the miRNA gene regulatory network. Results : In total, 36 DEGs, containing 27 up-regulated and nine down-regulated DEGs, were identified. GO functional analysis showed that these DEGs were involved in regulation of apoptotic process, cytoplasm, protein binding and other biological processes. KEGG enrichment analysis showed that these DEGs mediated signaling pathways, including human T-cell lymphotropic virus (HTLV)-I infection and microRNAs in cancer. The results of PPI network and cytohubba showed that there was a relationship between DEGs, and five hub genes related to stroke were obtained : SOCS3, KRAS, PTGS2, EGR1, and DUSP1. Combined with the visualization of DEG-miRNAs, hsa-mir-16-5p, hsa-mir-181a-5p and hsa-mir-124-3p were predicted to be the key miRNAs in stroke, and three miRNAs were related to hub gene. Conclusion : Thirty-six DEGs, five Hub genes, and three miRNA were obtained from bioinformatics analysis of IS microarray data, which might provide potential targets for diagnosis and treatment of IS.

• BMB Reports
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• 제50권11호
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• pp.590-595
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• 2017

High-mobility group box-1 (HMGB-1) is expressed in almost all cells, and its dysregulated expression correlates with inflammatory diseases, ischemia, and cancer. Some of these conditions accompany HMGB-1-mediated abnormal angiogenesis. Thus far, the mechanism of HMGB-1-induced angiogenesis remains largely unknown. In this study, we performed time-dependent DNA microarray analysis of endothelial cells (ECs) after HMGB-1 or VEGF treatment. The pathway analysis of each gene set upregulated by HMGB-1 or VEGF showed that most HMGB-1-induced angiogenic pathways were also activated by VEGF, although the activation time and gene sets belonging to the pathways differed. In addition, HMGB-1 upregulated some VEGFR signaling-related angiogenic factors including EGR1 and, importantly, novel angiogenic factors, such as ABL2, CEACAM1, KIT, and VIPR1, which are reported to independently promote angiogenesis under physiological and pathological conditions. Our findings suggest that HMGB-1 independently induces angiogenesis by activating HMGB-1-specific angiogenic factors and also functions as an accelerator for VEGF-mediated conventional angiogenesis.

• Asian-Australasian Journal of Animal Sciences
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• 제23권1호
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• pp.17-24
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• 2010

In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communications and interactions between oocytes and cumulus cells. However, information on stage specific gene expression in swine during the phase is not well understood. To investigate the function of cumulus cells during in vitro maturation of porcine oocytes and gene expression, suppression subtractive hybridization (SSH) was performed to screen genes that were differentially expressed between cumulus-oocyte complexes (COCs) and naked oocytes (NOs). Utilizing mRNAs from in vitro maturation oocytes, a SSH cDNA library from COCs as the tester and NOs as the driver was constructed. The SSH cDNA library was then screened using dot blot analysis. Results showed that a total of 70 clones randomly selected from the library were differentially expressed. Among these, 41 exhibited high homology to known genes and 11 were novel expressed sequences tags (ESTs). Four differentially expressed genes, including bfgf, sprouty 2, egr and btc, were further studied by real time quantitative PCR; results confirmed an increased expression of respective mRNA in COCs compared with NOs, which suggests that these factors may play an important role in oocyte development and maturation.

• 한국자동차공학회논문집
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• 제14권3호
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• pp.178-185
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• 2006

This paper investigates the steady-state combustion characteristics of the Homogeneous charge compression ignition(HCCI) engine with variable valve timing(VVT) and dimethyl ether(DME) direct injection, to find out its benefits in exhaust gas emissions. HCCI combustion is an attractive way to lower carbon dioxide($CO_2$), nitrogen oxides(NOx) emission and to allow higher fuel conversion efficiency. However, HCCI engine has inherent problem of narrow operating range at high load due to high in-cylinder peak pressure and consequent noise. To overcome this problem, the control of combustion start and heat release rate is required. It is difficult to control the start of combustion because HCCI combustion phase is closely linked to chemical reaction during a compression stroke. The combination of VVT and DME direct injection was chosen as the most promising strategy to control the HCCI combustion phase in this study. Regular gasoline was injected at intake port as main fuel, while small amount of DME was also injected directly into the cylinder as an ignition promoter for the control of ignition timing. Different intake valve timings were tested for combustion phase control. Regular gasoline was tested for HCCI operation and emission characteristics with various engine conditions. With HCCI operation, ignition delay and rapid burning angle were successfully controlled by the amount of internal EGR that was determined with VVT. For best IMEP and low HC emission, DME should be injected during early compression stroke. IMEP was mainly affected by the DME injection timing, and quantities of fuel DME and gasoline. HC emission was mainly affected by both the amount of gasoline and the DME injection timing. NOx emission was lower than conventional SI engine at gasoline lean region. However, NOx emission was similar to that in the conventional SI engine at gasoline rich region. CO emission was affected by the amount of gasoline and DME.

• Journal of Korean Neurosurgical Society
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• 제55권6호
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• pp.370-374
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• 2014

To present a case of cauda equina syndrome (CES) caused by chronic inflammatory demyelinating polyneuropathy (CIDP) which seemed clinically similar to Charcot-Marie-Tooth disease type1 (CMT1). CIDP is an immune-mediated polyneuropathy, either progressive or relapsing-remitting. It is a non-hereditary disorder characterized by symmetrical motor and sensory deficits. Rarely, spinal nerve roots can be involved, leading to CES by hypertrophic cauda equina. A 34-year-old man presented with low back pain, radicular pain, bilateral lower-extremity weakness, urinary incontinence, and constipation. He had had musculoskeletal deformities, such as hammertoes and pes cavus, since age 10. Lumbar spine magnetic resonance imaging showed diffuse thickening of the cauda equina. Electrophysiological testing showed increased distal latency, conduction blocks, temporal dispersion, and severe nerve conduction velocity slowing (3 m/s). We were not able to find genetic mutations at the PMP 22, MPZ, PRX, and EGR2 genes. The pathologic findings of the sural nerve biopsy revealed thinly myelinated nerve fibers with Schwann cells proliferation. We performed a decompressive laminectomy, intravenous IgG (IV-IgG) and oral steroid. At 1 week after surgery, most of his symptoms showed marked improvements except foot deformities. There was no relapse or aggravation of disease for 3 years. We diagnosed the case as an early-onset CIDP with cauda equine syndrome, whose initial clinical findings were similar to those of CMT1, and successfully managed with decompressive laminectomy, IV-IgG and oral steroid.

• 한국자동차공학회논문집
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• 제19권6호
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• pp.17-22
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• 2011

This study is to investigate the effect of the cetane number in ultra low sulfur diesel fuel on combustion characteristics and exhaust emissions at 1500 rpm and 2.6bar BMEP in low-temperature diesel combustion with 1.9L common rail direct injection diesel engine. Low-temperature diesel combustion was achieved by adopting external high EGR rate with the strategic injection control without modification of engine components. Test fuels are ultra low sulfur diesel fuel (sulfur less than 12 ppm) with two cetane numbers (CN), i.e., CN30 and CN55. For the CN30 fuel, as a start of injection (SOI) timing is retarded, the duration of an ignition delay was decreased while still longer than $20^{\circ}CA$ for all the SOI timings. In the meanwhile, the CN55 fuel showed that an ignition delay was monotonically extended as an SOI timing is retarded but much shorter than that of the CN30 fuel. The duration of combustion for both fuels was increased as an SOI timing is retarded. For the SOI timing for the minimum BSFC, the CN30 produced nearly zero PM much less than the CN55, while keeping the level of NOx and the fuel consumption similar to the CN55 fuel. However, the CN30 produced more THC and CO than the CN55 fuel, which may come from the longer ignition delay of CN30 to make fuel and air over-mixed.

• 대한기계학회논문집B
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• 제22권9호
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• pp.1317-1324
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• 1998

In general, DI gasoline engine has the advantages of higher power output, higher thermal efficiency, higher EGR tolerance and lower emissions due to the operation characteristics of increased volumetric efficiency, compression ratio and ultra-lean combustion scheme. In order to apply the concept of stratified charge into direct injection gasoline engine, some kinds of methodologies have been adapted in various papers. In this study, a reflector was adapted around the injector nozzle to apply the concept of stratified charge combustion which leads the air-fuel mixture to be rich near spark plug. Therefore, the mixture near the spark plug is locally rich to ignite while the lean mixture is wholly introduced into the combustion chamber. The characteristics of combustion is analyzed with the variations of fuel injection pressure and load in a stratified -charge direct injection single cylinder gasoline engine. The obtained results are summarized as follows ; 1. The MBT spark timing approached to TDC with the increase of load on account of the increase of evaporation energy, but has little relation with fuel injection pressure. 2. The stratification effects are apparent with the increase of injection pressure. It is considered by the development of secondary diffusive combustion and the increase of heat release of same region, but proceed rapidly than diesel engine. Especially, in the case of high pressure injection (l70bar) and high load (3.0kgf m), the diffusive combustion parts are developed excessively and results in the decrease of peak pressure than in the case of middle load. 3. The index of engine stability, COVimep value, is drastically decreased with the increase of load. 4. To get better performance of DI gasoline engine development, staged optimizaion must be needed such as injection pressure, reflector, intake swirl, injection timing, chamber shape, ignition system and so on. In this study, the I50bar injection pressure is appeared as the optimum.

• 한국운동역학회지
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• 제18권3호
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• pp.61-69
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• 2008

본 연구는 평균대에서 필수요구조건(EGR)에 해당되는 동작인 옆공중돌기 동작의 성공과 실패를 운동역학적으로 비교 분석하였다. 연구대상은 국가대표 선수들로 하였으며, 연구의 목적은 기술의 실수 요인을 규명하여 지도자 및 선수들에게 과학적으로 유용한 정보를 제공하는데 있다. 성공시 옆공중돌기 동작의 신체중심은 평균대 중앙 중심으로 좌-우축으로 벗어나는 결과가 나타나고 동작의 특성상 회전하는 방향으로 신체중심이 이동한다는 것을 나타내고 있다. 도약구간인 event2와 3에서 성공시 상 하 변화가 크게 나타나 체공시간을 높이는 것으로 투사변인이 실패시 보다 높게 나타나는 결과를 뒷받침 해 주고 있다. 또한 공중 비약을 위해 다리를 차는 동작을 하는 오른쪽 고관절 각도와 속도, 각속도가 실패시 보다 성공시 동작에서 모두 크게 나타나 도약의 최적조건을 수행하고 있다. 어깨를 중심으로 상지분절의 속도와 견관절 각속도를 크게 함으로써 운동량을 증가시켰으며 그중 오른쪽 견관절 각속도가 크게 나타난 이유는 오른쪽 다리를 잡기 위한 결과이다. 옆 공중돌기 동작에서 실패시 보다 성공시 동작에서 x축을 중심으로 각운동량이 크게 나타나 공중 비행 동작은 x축을 중심으로 각운동량을 크게 하는 것이 중요한 요인으로 나타났으며 옆공중돌기 특성상 y축과 z축으로 각운동량도 적정한 비율로 동작이 이루어져야 성공적인 동작을 만들수 있다.

• Journal of Plant Biology
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• 제36권4호
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• pp.363-370
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• 1993

예취 후 재생시 저장질소의 재이동과 재생기관으로서의 전이를 양적 평가하고 xylem을 통한 환원유기질소의 이동형태를 규명하기 위해 알팔파(Medicago sativa L. cv. Europe)를 수경재배하였다. 근류균을 형성하지 않은 알팔파에 예취 전 $^{15}N$ labeling을 실시하여 재생기간 중 각 기관내 분포된 $^{15}N$ dmlexcess를 분석하고 xylem sap을 채취하여 아미노산의 조성 및 함량을 분석하였다. 예취 후 잔여기관내의 $^{15}N$ 함량은 재생이 진행됨에 따라 유의적으로 감소하였고, 감소된 $^{15}N$은 질소의 전이에 의해 새로 재생되는 잎과 줄기에서 발견되었다. 저장기관으로부터 전이된 $^{15}N$의 약 2/3가 잎으로, 나머지가 줄기로 분포되어 잎이 줄기보다 저장질소에 대한 수용력(sink strength)이 높았다. 예취 후 재생초기 10일 동안 잎과 줄기의 재생에 이용된 무기질 질소의 함량은 낮은 반면, 이 기간동안 총 저장질소의 약 72%가 잎과 줄기로 전이되었다. 재생이 진행됨에 따라 무기질 질소의 이용율은 점차 증가하였다. 재생초기 10일 동안 뿌리조직(주근과 지근)의 아미노산태 및 단백질태 질소함량은 유의적으로 감소하였다. 주근과 지근에서 공히 단백질태 질소함량이 아미노산태 질소함량 보다 높았으나, 재생 10일 동안 전이된 비율은 아미노산태가 월등히 높았다. 이는 저장질소는 아미노산태로 우선적으로 이동하여 재생에 이용된다는 것을 보여준다. Asparagine이 xylem sap내 총 아미노산의 75%로서 환원태 유기질소의 주요 이동형태였다. 재생 10일 동안 asparagine의 상대적 함량은 59%까지 감소하였는데, 이러한 감소는 asparatate의 glutamine의 상대적 함량의 보충적 증가를 동반하였다.