• Molecules and Cells
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• 제37권1호
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• pp.24-30
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• 2014

Inductive expression of early growth response 1 (Egr-1) in neurons is associated with many forms of neuronal activity. However, only a few Egr-1 target genes are known in the brain. The results of this study demonstrate that Egr-1 knockout (KO) mice display impaired contextual extinction learning and normal fear acquisition relative to wild-type (WT) control animals. Genome-wide microarray experiments revealed 368 differentially expressed genes in the hippocampus of Egr-1 WT exposed to different phases of a fear conditioning paradigm compared to gene expression profiles in the hippocampus of KO mice. Some of genes, such as serotonin receptor 2C (Htr2c), neuropeptide B (Npb), neuronal PAS domain protein 4 (Npas4), NPY receptor Y1 (Npy1r), fatty acid binding protein 7 (Fabp7), and neuropeptide Y (Npy) are known to regulate processing of fearful memories, and promoter analyses demonstrated that several of these genes contained Egr-1 binding sites. This study provides a useful list of potential Egr-1 target genes which may be regulated during fear memory processing.

• BMB Reports
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• 제53권12호
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• pp.628-633
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• 2020

WNT11 is a member of the non-canonical Wnt family and plays a crucial role in tumor progression. However, the regulatory mechanisms underlying WNT11 expression are unclear. Tumor necrosis factor-alpha (TNFα) is a major inflammatory cytokine produced in the tumor microenvironment and contributes to processes associated with tumor progression, such as tumor invasion and metastasis. By using site-directed mutagenesis and introducing a serial deletion in the 5'-regulatory region of WNT11, we observed that TNFα activates the early growth response 1 (EGR1)-binding sequence (EBS) in the proximal region of WNT11 and that the transcription factor EGR1 is necessary for the TNFα-induced transcription of WNT11. EGR1 bound directly to the EBSs within the proximal 5'-regulatory region of WNT11 and ectopic expression of EGR1 stimulated WNT11 promoter activity, whereas the knockdown of EGR1 expression by RNA interference reduced TNFα-induced WNT11 expression in T47D breast cancer cells. We also observed that mitogen-activated protein kinases (MAPK), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 kinase mediated TNFα-induced transcription of WNT11 via EGR1. Our results suggest that EGR1 directly targets WNT11 in response to TNFα stimulation in breast cancer cells.

• Journal of Advanced Marine Engineering and Technology
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• 제22권6호
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• pp.827-835
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• 1998

The effects of recirculated exhaust gas on the wear of cylinder liner piston and piston rings have been investigated by the experiment with a two-cylinder four cycle indirect injection diesel engine operating at 75% load and 1600 rpm speed For the purpose of comparison between the rates of two cylinders with and without EGR the recirculated exhaust gas is sucked into one of two cylinders after the soot among exhaust emissions is removed by an intntionally designed cylinder-type scrubber equipped with 6 water injectors(A water injector has 144 nozzles of 1.0 mm diame-ter) while only the fresh air into another cylinder. These experiments are carried out on the fuel injection at a fixed $15.3^{\circ}$ BTDC timing. It is found that firstly the mean wear amount of cylinder liner with EGR is more increased in the measurement positions of the second half than of the first half and the mean wear amount without EGR is almost uniform regardless of measurement posi-tions secondly the wear rates of the first and second piston ring(compression ring)thickness with EGR are more than twice but the wear rate of oil ring thickness without EGR is more increased than that with EGR and finally the wear rate of piston skirt with EGR is a little bit increased but the piston hed diameter is rather increased owing to soot adhesion and corrosion wear and espe-cially larger with EGR.

• Journal of Advanced Marine Engineering and Technology
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• 제24권2호
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• pp.57-62
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• 2000

The effect of exhaust gas recirculation(EGR) on the characteristics of exhaust gas emissions, and SFC are experimentally investigated by four-cylinder, four-cycle and direct injection marine diesel engine. In order to reduce the soot contents in the recirculated exhaust gas to intake system of the engines, a soot removal system of a cylinderical-type scrubber is specially designed and manufactured for the experimental system. (1) SFC is increased in downward convex curve style with increasing excess air ratio, it is increased with increasing EGR rate at the same excess air ratio. (2) NOx emission is reduced in downward convex curve style with increasing excess air ratio, it is reduced with increasing EGR rate at the same excess air ratio. (3) Soot emission is decreased in downward convex curve style with increasing excess air ratio, it is reduced with increasing EGR rate at the same excess air ratio. (4) CO emission is increased in nearly straight line style with increasing excess air ratio, it is increased with increasing EGR rate at the same excess air ratio. (5) HC emission is not constant tendency with increasing excess air ratio, it is increased with increasing EGR rate at the same excess air ratio.

• Journal of Advanced Marine Engineering and Technology
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• 제22권4호
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• pp.481-489
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• 1998

The effects of recirculated exhaust gas on the characteristics of fuel economy combustion and exhaust emissions have been experimentally investigated by a four-cylinder four cycle indirect injection water-cooled and marine diesel engine operating at several loads and speeds. in order to reduce the soot contents in the recirculated exhaust gas to intake system of the engine a novel diesel soot removal system with a cylinder-type scrubber which has 6 water injectors(A water injector has 144 nozzles in 1.0 mm diameter) is specially designed and manufactured for the experiment system The experiments in this study are performed at the fixed fuel injection timing of $15.3^{\circ}$ BTDC regardless of experimental conditions, The brake specific fuel consumption rate is slightly fluctuated with EGR in the range of experimental conditions, The maximum value of premixed combustion for the rate of heat release is decreased with EGR at engine load 25% and the ignition is slightly delayed with EGR at engine load 100% NOx emissions are markedly decreased with EGR especially at high loads while soot emissions are increased as the EGR rate rises.

• Journal of Advanced Marine Engineering and Technology
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• 제33권6호
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• pp.896-902
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• 2009

EGR is applied in order to lower temperature of combustion chamber by using the specific heat of carbon dioxide in engine exhaust gas. However, the problem of EGR system in diesel engine is high PM concentration. Combined EGR system can be reduced it by mixing exhaust gas of gas engine into the intake air of diesel engine. This experimental study was designed for EGR system for both engines use. The results of EGR experimental study by using diesel engine and gas engine are as follows. 1) The pressure of combustion and rate of heat release decreased. 2) The specific fuel consumption increased. But, up to middle load, it little increased. 3) NO concentration has decreased up to 50% in almost all combustion area. 4) The variation of the PM concentration at low load is not so seen. But at high load, PM increased rapidly when concentration of oxygen is decreased and most of it caused the increasing of Dry Soot.

• 한국자동차공학회논문집
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• 제3권1호
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• pp.76-88
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• 1995

The effects of exhaust gas recirculation(EGR) on the characteristics of NOx emissions and specific fuel consumption rate have been investigated using an eight-cylinder. four cycle. direct injection diesel engine operating at several loads and speeds. The theoretical NO formation concentration is calculated with the equivalence ratio as a parameter of flame temperature to study the effect of EGR on NOx emissions in the diesel combustion. The experiments in this study are conducted on the fixed fuel injection timing of $38^{\circ}$ BTDC regardless of experimental conditions. It is found that the specific fuel consumption rate is slightly increased with EGR rate. and NOx emissions are markedly reduced owing to the drop of the incoming oxygen concentratio and the increase of equivalence ratio as the EGR rate increases.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2009년도 추계학술발표논문집
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• pp.534-537
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• 2009

최근 자동차 배기가스 규제가 더욱 강화됨에 따라 완전 연소를 통한 배기가스 저감에 관하여 많은 연구가 진행되고 있다. 특히, 고온 연소시 문제가 되는 질소 산화물의 저감을 위해 많은 노력을 기울이고 있다. 질소 산화물을 저감하는데 효과적인 EGR 기술의 도입이 보편화되고 있지만, 각 실린더별 EGR 분배 특성에 따라 엔진의 성능이 달라지는 역효과가 발생한다. 시제품을 동한 EGR 분배특성 파악은 비용과 시간 측면에서 상당한 난제가 산재해있다. 본 연구에서는 이러한 실험시간과 비용 절감을 위하여 1D 성능해석 프로그램인 GT-Power를 적용 EGR의 분배특성을 예측하였다.

• 한국자동차공학회논문집
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• 제14권5호
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• pp.58-64
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• 2006

Homogeneous charge compression ignition(HCCI) combustion is an advanced technique for reducing the hazardous nitrogen oxide(NOx) and particulate matter(PM) in a diesel engine. NOx could be reduced by achieving lean homogeneous mixture resulting in combustion temperature. PM could be also reduced by eliminating fuel-rich zones which exist in conventional diesel combustion. However previous researches have reported that power-output of HCCI engine is limited by the high intensive knock and misfiring. In an attempt to extend the upper load limit for HCCI operation, supercharging in combination with Exhaust Gas Recirculation(EGR) has been applied: supercharging to increase the power density and EGR to control the combustion phase. The test was performed in a single cylinder engine operated at 1200 rpm. Boost pressures of 1.1 and 1.2 bar were applied. High EGR rates up to 45% were supplied. Most of fuel was injected at early timing to make homogeneous mixture. Small amount of fuel injection was followed near TDC to assist ignition. Results showed increasing boost pressure resulted in much higher power-output. Optimal EGR rate influenced by longer ignition delay and charge dilution simultaneously was observed.

• Journal of Advanced Marine Engineering and Technology
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• 제24권3호
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• pp.70-78
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• 2000

The effects of recirculated exhaust gas on the characteristics of ;$NO_x$ and soot emissions under a wide range of engine load have been experimentally investigated by a water-cooled, four-cylinder, indirect injection, four cycle and marine diesel engine operating at two kinds of engine speeds. The purpose of the present study is to develop the EGR control system for reducing $NO_x$ and soot emissions simultaneously in diesel engines. The EGR system is used to reduce NOx emissions, and a novel diesel soot removal apparatus with a cylinder-type scrubber for the experiment system which has 6 water injectors(A water injector is made up 144 nozzles with 1.0mm in diameter) is specially designed and manufactured to reduce the soot contents in the recirculated exhaust gas to intake system of the engines. The intake oxygen concentration obtained by the intake air flow and the oxygen concentration in the recirculated exhaust gas, and the exhaust oxygen concentration measured in exhaust manifold are used to analyse and discuss the influences of EGR on NOx and soot emissions. The experiments are performed at the fixed fuel injection timing of $15.3^{\circ}$ BTDC regardless of experimental conditions. It is found that $NO_x$ emissions decrease and soot emissions increase owing to the drop of intake oxygen concentration and exhaust oxygen concentration as EGR rate rises. Also, one can conclude that it is sufficient for the scrubber EGR system with a novel diesel soot removal apparatus to reduce $NO_x$ emissions, but not to reduce soot emissions.