• The Plant Pathology Journal
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• 제32권3호
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• pp.216-227
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• 2016

Biological control agents (BCAs) from different microbial taxa are increasingly used to control bacterial wilt caused by Ralstonia solanacearum. However, a quantitative research synthesis has not been conducted on the role of BCAs in disease suppression. Therefore, the present study aimed to meta-analyze the impacts of BCAs on both Ralstonia wilt disease suppression and plant (host) growth promotion. The analysis showed that the extent of disease suppression by BCAs varied widely among studies, with effect size (log response ratio) ranging from -2.84 to 2.13. The disease incidence and severity were significantly decreased on average by 53.7% and 49.3%, respectively. BCAs inoculation also significantly increased fresh and dry weight by 34.4% and 36.1%, respectively on average. Also, BCAs inoculation significantly increased plant yield by 66%. Mean effect sizes for genus Pseudomonas sp. as BCAs were higher than for genus Bacillus spp. Among antagonists tested, P. fluorescens, P. putida, B. cereus, B. subtilis and B. amyloliquefaciens were found to be more effective in general for disease reduction. Across studies, highest disease control was found for P. fluorescens, annual plants, co-inoculation with more than one BCA, soil drench and greenhouse condition were found to be essential in understanding plant responses to R. solanacearum. Our results suggest that more efforts should be devoted to harnessing the potential beneficial effects of these antagonists, not just for plant growth promoting traits but also in mode of applications, BCAs formulations and their field studies should be considered in the future for R. solanacearum wilt disease suppression.

• 한국축산식품학회지
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• 제30권4호
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• pp.575-581
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• 2010

This investigation was aimed at developing a ready-to-reconstitute beverage by utilizing probiotics and whey protein hydrolysates carrying bioactive peptides. Cheddar cheese whey was ultrafiltered. The 18% protein retentate was subjected to protein hydrolysis using Neutrase. The hydrolyzed retentate was further condensed to 35% total solids and spray-dried at $75^{\circ}C$ outlet air temperature. Different levels of sugar, citric acid and stabilizer were blended for spray-dried hydrolysates. Spray-dried hydrolysate was further inoculated with different levels of probiotics grown in a whey medium and dried in fluidized-bed drier at $40^{\circ}C$ to obtain a ready-to-reconstitute beverage. Hydrolysis was greatest at an enzyme:substrate ratio of 1:25 for 3 h. Spray-dried hydrolysate reconstituted to 1% protein and blended with 15% sugar, 0.2% citric acid and 0.15% xantham gum resulted in a superior product with no sedimentation. Accordingly, sugar, citric acid and xanthum gum were dry-blended with spray-dried hydrolysates. Bifidobacterium bifidum and Lactobacillus acidophilus that was grown separately in a whey medium, blended to produce 2% spray-dried hydrolysate and dried as described above resulted in a readyto-reconstitute beverage mix. The fluidized dried product typically exhibited a probiotic count of $10^8$colony forming units (CFU)/g. However, blending of probiotic to the retentate and direct spray-drying precipitously reduced the probiotic count to $10^4$ CFU/g of powder.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.1011-1018
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• 2015

This study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of Opisthorchis viverrini (OV)-related cholangiocarcinoma (CCA). Plasma phosphoproteomes from CCA patients (10) and non-CCA subjects (5 each for healthy subjects and OV infection) were investigated using gel-based and solution-based LC-MS/MS. Phosphoproteins in plasma samples were enriched and analyzed by LC-MS/MS. STRAP, PANTHER, iPath, and MeV programs were applied for the identification of their functions, signaling and metabolic pathways; and for the discrimination of potential biomarkers in CCA patients and non-CCA subjects, respectively. A total of 90 and 60 plasma phosphoproteins were identified by gel-based and solution-based LC-MS/MS, respectively. Most of the phosphoproteins were cytosol proteins which play roles in several cellular processes, signaling pathways, and metabolic pathways (STRAP, PANTHER, and iPath analysis). The absence of serine/arginine repetitive matrix protein 3 (A6NNA2), tubulin tyrosine ligase-like family, member 6, and biorientation of chromosomes in cell division protein 1-like (Q8NFC6) in plasma phosphoprotein were identified as potential biomarkers for the differentiation of healthy subjects from patients with CCA and OV infection. To differentiate CCA from OV infection, the absence of both serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform and coiled-coil domain-containing protein 126 precursor (Q96EE4) were then applied. A combination of 5 phosphoproteins may new alternative choices for CCA diagnosis.

• 한국수산과학회지
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• 제22권5호
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• pp.332-341
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• 1989

경상남도 일광 연안으로 흘러내리는 하천의 하구 기수역에서 미끈날망둑온 채집하여 이석과 위내용물을 분석하여, 자치어기의 나이, 성장율 및 식성을 추정하였다. 이석 미세성장선의 하루주기성은 약 15일 간격으로 연속하여 채집된 어류 이석을 각 채집일을 감안하여 성장선 배열의 공통성을 비교 분석하여 증명하였다. 체장의 성장은 Gompertz의 식으로 유의하게 표시되었다. $L=5.73{\cdot}\;e^{15.06}(1-e^{-0.0015t})$관찰 범위에서 체장의 일일성장율은 부화 후 $50\~60$일 사이에는 0.40 mm/day로 작았으나 자라면서 빨라져 $90\~100$일 사이에는 0.85 mm/day로 증가하였다. 위내용물 분석 결과, 미끈날망둑은 전형적인 육식성 어종으로 밝혀졌는데, 주된 먹이생물은 polychaetes, amphipods, copepods 였으며 이들은 위내용물의 대부분을 차지하였다. 미끈날망둑은 성장에 따라 다소 먹이 선택에 있어 변화를 보이는데, $15\~30$mm 크기군에서는 copepods가 비교적 많이 섭이 되었지만, 성장함에 따라 amphipods의 섭이가 증가되었다. 한편 polychaetes의 선호도는 모든 크기군에 걸쳐 가장 높았다.

• Fisheries and Aquatic Sciences
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• 제19권9호
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• pp.41.1-41.7
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• 2016

Background: The transgenic approach using estrogen-responsive regulator in fish has been given much attention as a potential means to detect and/or address estrogen-related aquatic pollutions. In order to address the development stage- and reproduction status-dependent expression patterns of the chgH-rfp transgene (red fluorescent protein transgene driven by choriogenin H promoter) in marine medaka Oryzias dancena, naturally occurring red fluorescent protein (RFP) signals under non-exposed conditions as well as the transgenically induced RFP signals under estrogen-exposed conditions were assayed. Results: Female transgenics begun to show naturally occurring RFP signals from the age of 7 weeks post hatching (WPH) without experimental estrogen exposure. Afterward, these RFP signals in female transgenics became robust with the progress of ovarian maturation. On the other hand, male transgenics did not show any naturally occurring RFP signal under non-exposed conditions irrespective of developmental stages and maturation statue. Upon exposures using estradiol-$17{\beta}$ (E2) and $17{\alpha}$-ethinylestradiol (EE2), RFP signals were significantly induced specifically in the livers of transgenic males. Conclusions: Male chgH-rfp transgenics were able to keep the "off" state of RFP expression during their entire life cycle unless exposed to exogenous estrogens. Owing to their tight regulation capability of estrogen-responsive transgene, transgenesis of chgH-rfp in male marine medaka could offer a useful model system for future ecotoxicogenomic studies regarding estrogenicity-related issues in aquatic and marine environments.

• Clinical and Experimental Pediatrics
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• 제53권2호
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• pp.244-247
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• 2010

자발성 종격동 기흉은 신생아기에 발생하는 호흡곤란의 드문 원인 중의 하나이며 대부분의 증례에서는 인공호흡, 출산손상, 태변흡입 등이 선행하는 경우가 많으며, 또한 대다수가 미숙아로 알려져있다. 저자들은 신생아 진찰 중 심음이 적게 들리는 소견으로 발견되었던, 위와 같은 위험인자가 없는 건강한 만삭아에서 발생한 자발성 종격동 기흉 1예를 경험하였기에 이를 보고하는 바이다.

• 한국약용작물학회지
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• 제21권2호
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• pp.105-111
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• 2013

This study was performed to investigate the enhancement of immunomodulatory activities of Lithospermum erythrorhizon by extreme process. The extracts are WE100 (water extract for 24 hours at $100^{\circ}C$), WE80 (water extract for 24 hours at $80^{\circ}C$), EE (70% ethyl alcohol extract for 24 hours at $80^{\circ}C$) and EPE (extreme process for 30 minutes at $25^{\circ}C$, 500 MPa after 70% ethyl alcohol extracts for 3 hours at 40, 50, $60^{\circ}C$). Extraction yield was increased up to 5~10% by extreme process, compare to the normal extraction such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts was showed in the range of 12.68~15.89% at $1.0mg/m{\ell}$ for human lung cell (HEL299). The EPE40 was showed the lowest cytotoxicity 12.68%. The EPE60 extracted by extreme process increased the growth of human B and T cells up to $12.12{\times}10^4\;cells/m{\ell}$ and $14.88{\times}10^4\;cells/m{\ell}$, respectively and the EPE60 greatly increased the cytokine secretion of both IL-6 and TNF-${\alpha}$. The extracts by extreme process also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It can be concluded that Lithospermum erythrorhizon has immune activities and The extreme process could increase higher immune activities possibly by immunomodulatory compounds.

• 한국항공우주학회지
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• 제43권2호
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• pp.179-185
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• 2015

우주용 고해상도 전자광학카메라의 개발에 있어 초점면 배열 장치(Focal Plane Assembly, FPA)의 열제어 설계는 영상품질을 결정하는 중요한 요소이다. FPA 열제어 설계의 목표는 영상 촬영 기간 중 안정적인 열 환경을 제공하는 것으로, 촬영 기간 동안 운용 온도를 주어진 범위 내로 유지해야 하며 후속 촬영에 문제가 없도록 빠른 시간 내에 온도 초기화를 완료해야 한다. 상기의 두 항목인 온도 유지와 온도 초기화 중에서 FPA 구조 설계에 따른 영향을 가장 많이 받으며, 설계 변경의 자유도가 상대적으로 낮은 온도 유지 항목에 대하여 FPA 구조설계 초기 단계부터 검토 및 반영이 가능한 단순화 된 열 저항 해석 기반의 온도 예측 방안을 정의하였다. 또한 열해석 결과와의 비교를 통해 온도 예측 방안을 검증, FPA 설계를 위한 trade-off에 효과적으로 사용할 수 있음을 확인하였다.

• 기술혁신학회지
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• 제16권2호
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• pp.424-443
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• 2013

교육과학기술부와 한국연구재단은 창의적 도전적 연구를 적극 장려하고자 2012년 기초연구사업 중점 추진방향으로 '한국형 그랜트' 지원 방식을 도입하였으며, 이에 따라 연구자가 추후 과제 신청시 기존 연구수행 성과를 집중적으로 평가 받게 되었다. 이에, 선정평가에서 과제관리 및 종료평가에 이르는 전주기적 성과관리 시스템의 필요성이 크게 대두되었다. 본 연구에서는 전주기적 성과관리 시스템의 첫 단계로 분야별 연구실적의 정량적 가이드라인를 제시하여 연구역량평가의 보조적 수단으로 사용할 방안을 도출하고자 하였다. 그 결과, 상위그룹 간 비교 시에는 순위보정영향력지수(Ranked Normalized Impact Factor, rnIF), 하위그룹 간 비교 시에는 영향력지수(Impact Factor, IF) 또는 rnIF를 정량가이드라인으로 제안하였다. SCI급 논문 수는 동일 분류 내 위상비교 또는 동일 위상내 분류별 비교 등에 사용할 것을 제안하였다.

• 한국산업보건학회지
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• 제30권2호
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• pp.196-204
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• 2020

Objective: Chronic obstructive pulmonary disease(COPD) is characterized by persistent airflow limitations associated with chronic inflammatory response due to noxious particles or gases in the lung. Increasing oxidative stress associated with COPD. The aim of this study was to evaluate the influencing factors of biomarkers for oxidative stress in retired miners with COPD. Methods: The levels of total peroxide(TPx), total antioxidant capacity(TAC), and oxidative stress index(TPx/TAC ratio, OSI) in plasma as biomarkers for oxidative stress, serum C-reactive protein(CRP) as a biomarker for inflammation, and general characteristics were measured in 93 male subjects with COPD. COPD was defined as post bronchodilator FEV₁/FVC<0.7 by spirometry. Results: Mean levels of TPx(p=0.013), TAC(p=0.010), OSI(p=0.040), and CRP(p=0.024) were higher in current smokers. Levels of TPx(β=0.445, p<0.001), TAC(β=0.490, p<0.001), and OSI(β=0.351, p<0.001) were related to CRP levels, and CRP levels were related to %FEV₁ predicted(β=-0.295, p=0.003) and current smoking(β=0.214, p=0.032). Conclusions: These results suggest that oxidative stress was related to inflammation, and inflammation were related to decreasing %FEV₁ predicted and current smoking in retired miners with COPD.