• BMB Reports
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• 제29권1호
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• pp.17-21
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• 1996

Ile91 of restriction endonuclease EcoRV, which has not been known to take part directly in catalytic activity, was substituted with Leu by site-directed mutagenesis. The Ile91Leu mutant shows over 1000-fold less activity than the wild type EcoRV under standard reaction condition. The metal ion dependency of the reaction was altered. In contrast to the wild type EcoRV, the mutant prefers $Mn^{2+}$ to $Mn^{2+}$ as the cofactor. In $Mn^{2+}$ buffer the mutant is as active as the wild type enzyme in $Mn^{2+}$ buffer. Like the wild type enzyme, the mutant shows an unspecific binding of DNA in gel shift experiments. In contrast to the wild type enzyme, the mutant did not cleave at noncognate sites of DNA under star condition.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제1권2호
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• pp.99-103
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• 1997

The bacteriophages lytic for Vibrio furnissi, Vibrio fluvialis and Vibrio parahaemolyticus were isolated from fish gills and shellfish. Nucleic acid of bacteriophages was prepared and restriction endonuclease profile was compared. All isolates contained deoxyribonucleic acid. V. furnissi bacteriophage from fish gills showed 2 bands with Bgl II, 1 with Pst, 3 with Hind III, I with Bam HI and 2 with EcoR 1. V fluvialis phage represented 7 fragments with Bgl II, 1 with Pst, 4 with Hind III, and 2 with EcoR 1. V. parahamolyticus produced 13 sites with Hind III and 4 sites with EcoR 1. The fragment types were varied depending on the phage isolation. All three phages were digested with Hind III and EcoR I with different sizes. V. furnissi phage were digested with 5 different restriction enzymes.

• Environmental Engineering Research
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• 제14권2호
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• pp.102-110
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• 2009

Application of artificial intelligence (AI) approaches in eco-environmental modeling has gradually increased for the last decade. Comprehensive understanding and evaluation on the applicability of this approach to eco-environmental modeling are needed. In this study, we reviewed the previous studies that used AI-techniques in eco-environmental modeling. Decision Tree (DT) and Artificial Neural Network (ANN) were found to be major AI algorithms preferred by researchers in ecological and environmental modeling areas. When the effect of the size of training data on model prediction accuracy was explored using the data from the previous studies, the prediction accuracy and the size of training data showed nonlinear correlation, which was best-described by hyperbolic saturation function among the tested nonlinear functions including power and logarithmic functions. The hyperbolic saturation equations were proposed to be used as a guideline for optimizing the size of training data set, which is critically important in designing the field experiments required for training AI-based eco-environmental modeling.

• 한국콘텐츠학회논문지
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• 제12권11호
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• pp.458-468
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• 2012

본 연구의 목적은 U-City와 Eco City에서 파생된 U-Eco City의 계획요소를 도출하고, 이들 계획요소로부터 도출된 서비스의 공간적용성을 분석하는데 있다. U-Eco City 서비스의 공간적용성을 분석하기 위하여 먼저 도시공간을 '건물', '가로', '시설' 그리고 '지구'의 4개 위계로 구분하였다. 이들 공간은 각각의 용도 등에 따라 보다 세분화한 후 분석에 적용하였다. U-Eco City의 계획요소 중 U-City의 계획요소는 도시의 활동에 따라 생활(Living), 일(Working), 이동(Moving) 및 놀이(Playing) 부문별로 총 228개 단위 서비스를 도출하였다. Eco City 서비스는 태양과 빛, 물, 녹지, 공기와 바람, 토지와 토양, 식생과 생태 등 자연요소와 에너지, 환경오염, 소음, CO2 및 가스, 환경폐기물 그리고 경관 등 자연요소 55개 부문에 대하여 도출하였다. U-Eco City 각 계획요소의 공간적용성은 U-City 및 Eco City 계획요소가 어떤 공간단위에 적용되는지를 교차분석(Cross-tabulation)을 통해 분석하였다. 분석결과, U-City 계획요소는 도시공간 중 '지구' 단위(35.7%), Eco City 계획요소는 '건물' 단위(38.8%)에 적용될 가능성이 가장 높은 것으로 분석되었으며, 각 계획요소의 공간적용성은 공간의 위계에 따라 각각 다르게 나타났다. 따라서 U-City와 Eco City계획요소가 공간에서 융합된 U-Eco City를 구현하기 위해서는 공간위계에 따른 차별적 계획요소를 도입하고 이에 대한 구체적 계획 및 설계지침이 제시되어야 할 것으로 판단된다.

• 자원리싸이클링
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• 제26권2호
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• pp.11-17
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• 2017

시멘트 공정에서는 산업부산물 및 생활폐기물 적용에 따른 여러 문제점들이 발생한다. 이들 폐기물 사용에 따라 발생하는 염소 바이패스 더스트의 주성분은 칼륨과 염소이며, 소량의 중금속이 함유되어 있다. 따라서 폐기물 재활용 측면에서, 염소 바이패스 더스트 내 중금속 제거가 필요하다. 본 연구에서는 염소 바이패스 더스트를 용해하여 제조되는 염화칼륨 내 중금속 제거 실험을 진행하였다. 최적의 중금속 제거 조건을 도출하기 위해 증류수 함량, 침전제 투입량을 제어하였다. 이에 따라 제조된 염화칼륨 분말 내 존재하는 중금속 종류 함량 등을 분석하였다. 침전제 투입량 증가에 따라 중금속 Pb의 함량이 감소하였다. A더스트와 증류수의 배합비 1:2, 침전제(NaOCl) 3%, B 더스트와 증류수의 배합비 1:2, 1:3.5, 침전제 3% 조건에서 중금속 Pb, Cd 및 As가 모두 불검출 되었다.

• 복식문화연구
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• 제21권2호
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• pp.195-205
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• 2013

Eco T-shirts have become a part of environmental campaigns following social trends toward eco-friendly designs. With the demand for such designs expected to grow, there has arisen a need for higher product variety. In the past, eco T-shirts were designed to convey messages about the protection of nature through the use of environmentally friendly text or images. However, in order to respond to the preferences and emotional needs of Koreans, designs should cater to their characteristics and tastes. To this end, this study used a Whan-ki Kim-inspired motif to develop an eco-friendly design tailored to Korean perspectives. It is believed that this process can not only result in a distinctive eco T-shirt design, but also make it globally competitive. With regard to research methodology, a total of 41 paintings of Whan-ki Kim were analyzed and classified into four major themes or key values expressed in modern eco-friendly fashion designs: naturalness, indigenousness, harmony between nature and man, and harmony between natural and artificial beauty. Employing a wide range of CAD techniques and varying forms, the themes were developed into 28 T-shirt designs after their forms, tones, and textures were fully explored. This study demonstrates the possibility of designing creative, aesthetic, and high value-added eco T-shirts through motif development. In addition, it successfully integrates Korean paintings in the development of T-shirt designs aimed at communicating environmentally friendly messages.

• 대한산업공학회지
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• 제40권2호
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• pp.223-232
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• 2014

Life-cycle assessment (LCA) is often employed to quantify the environmental impact of a product in a comprehensive manner. The aspects of performance and usability as well as that of eco-friendliness should be considered in an integrated manner for the market competitiveness of an eco-friendly product. The present study developed a product improvement plan for an eco-friendly electric heater by benchmarking two small-size electric heaters (companies 'H' and 'T') in terms of performance, usability, and eco-friendliness. The performance measurements such as temperature, humidity, wind speed, noise, and power consumption were collected while the two heaters were operated in a laboratory setting. Then, the usability evaluations such as aesthetics, operation satisfaction, performance satisfaction, and overall satisfaction were surveyed for the two heaters using a 5-point scale (1 for very unsatisfied and 5 for very satisfied). Lastly, the LCA analysis was conducted by following the six-step process of eco-friendly product design provided by KEITI. The analysis results of the two products being integrated with the aspects of product, service, and user, four design improvement directions such as eco-efficient, smart, modularized, and user-support were recommended for an eco-friendly electric heater. These proposed concepts would be useful to develop an eco-friendly electric heater design with a high level of market competitiveness.

• Journal of Microbiology
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• 제45권2호
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• pp.153-157
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• 2007

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

• 교육녹색환경연구
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• 제12권1호
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• pp.25-34
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• 2013

As the construction industry develops, environmental pollution gets increasingly serious, giving damage including the increase of incidence of respiratory diseases and skin diseases among children with weakened immune systems, rather than adults. In daycare centers, infants and children spending much of their time, have high frequency of contact with the interior floor finish material. However, the majority of the child care centers don not use eco-friendly flooring but ordinary monorium flooring, because the initial investment cost of the eco-friendly flooring is higher than ordinary monorium flooring. Therefore, in this study, life cycle costs including the initial investment cost of the eco-friendly flooring and ordinary monorium flooring were calculated, demonstrating that the eco-friendly flooring is more economical than ordinary monorium flooring in terms of life-cycle cost. In addition, the analysis of the environmental performance also showed the excellence of the eco-friendly floor finishes. It is expected that the use of the eco-friendly floor finishes will increase due to their excellence in the aspect of life cycle cost and eco-friendly performance, through this study.

• 한국주조공학회지
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• 제30권6호
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• pp.224-230
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• 2010

To prevent oxidation of Mg melt, $SF_6$ gas has been generally used for Mg alloys during melting and casting as a cover gas. The use of $SF_6$ gas, however, will be restricted owing to its crucial impact on global warming. Non-$SF_6$ process during melting and casting in diecasting industry has been proved with Eco-Mg alloys by a simple addition of small amount of CaO into Mg alloys. This paper shows non-$SF_6$ diecasting procedures for 0.7wt% CaO added AZ91D Eco-Mg alloys. Cold-chamber diecasting was performed under $CO_2$ atmosphere without $SF_6$ gas. An increment of mechanical properties, especially strength and ductility of Eco-Mg alloys is, in part, due to high-quality melt, refined grain size and $Al_2Ca$ second phase strengthening. Microstructures and mechanical properties of 0.7wt% CaO added AZ91D Eco-Mg alloys are evaluated in comparison with those of conventional AZ91D Mg alloy.