• Proceedings of the Polymer Society of Korea Conference
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• 2006.10a
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• pp.46-47
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• 2006

Vascularization is essential for success in regenerative medicine. We have developed in vitro models to study how human microvascular endothelial cells (EC) and endothelial progenitor cells (EPC) colonize polymer scaffolds and express the endothelial phenotype, including angiogenesis. Examples are given of supportive growth and differeniation of EC on microfibre meshes of the silk protein fibroin and blends of starch with poly(epsilon-caprolactone), phenotypic markers being studied at both protein and mRNA level. Experimental models are also shown and concepts discussed to investigate how the stem cell niche, including that responsible for vascularization could be targeted, for example, by using engineered biodegradable polymer nanoparticles.

• Journal of Food Hygiene and Safety
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• v.6 no.3
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• pp.133-137
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• 1991

In order to increase the expression of XMP aminase [EC 6.3.4.1], which catalizes the conversion of 5'-XMP to the DNA fragment containing gua A gene coding for XMP aminase from pLC 34-10 plasmid was subcloned into pBR 322, and 1.7 kb gua A gene fragment was recloned under the control of trp promoter of pDR 720, E. coli expression vector. XMP aminase activity had increased by about 17 times when compared with that of the strain earring pLC 34-10.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.10a
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• pp.297-304
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• 1999

the devastating earthquake which occurred in Turkey on August 187, 1999, reminds again us the necessity of the preparedness for the earthquake hazard. the level of earthquake engineering in Koran is still low due to the short period of the research and development in this field. Therefore the most efficient way of promoting our technology in this field is considered to collect the information on the state of the art and practice developed in the advanced countries in earthquake engineering and to analyze the advantages and disadvantages and then reflect these to the new seismic codes. for this purpose this study reviews the concept and main characteristics of Eurocode 8 and the findings of many researches that have been performed for the improvement of EC8. Finally the seismic performance evaluation of the building structures designed according to EC8. and the relevant recommendations are summarized.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.371-376
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• 2003

The objective of this paper is to compare the site-to-site variability of ISO 10844 pass-by-noise test sites. In order to investigate the site-to-site variance of test surfaces, European commercial tires are tested at seven different test sites. Three Korea test sites and four Europe test sites are selected.. The pass by noise test is executed according to a 2001/43/EC regulation. A]though the ISO surface has a very specific track composition, it does not reduce the variation of pass-by-noise measurements over the surface of test sites. This paper shows that the test results of pass-by-noise level are different depending on the test sites. The correlation obtained in this work is able to predict the pass by noise level for certain test site using the data measured from another test site. 17he prediction value is range with an error within 1dB(A).

• Korean Journal of Environment and Ecology
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• v.24 no.2
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• pp.209-221
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• 2010

This study was carried out to apply basic data of the native trees for planting in the salinity area by the vegetation ecological selection. Which focused on native woody species to the tolerances of saline soil and salt spray drift on the coastal forests in the West-Sea, Korea. The soil salinity($EC_{1:5}$) was 0.11dS$m^{-1}$, ranging of 0.00dS$m^{-1}$~0.68dS$m^{-1}$. The soil salinity was gradually decreasing from Belt I to Belt Ⅳ except the Belt I in some coastal windbreaks. The order of decreasing soil salinity was Belt I>Belt II>Belt III>Belt Ⅳ and the soil salinity was $EC_{1:5}$ 0.14dS$m^{-1}$, 0.11dS$m^{-1}$, 0.10dS$m^{-1}$, and 0.08dS$m^{-1}$, respectively. The total 181 taxa consisted of 52 families, 104 genus, 157 species, and 24 varieties were recorded as the trees tolerating to both soil salinity and salt spray drift. The trees emerged in the highest degree of salinity($EC_{1:5}$ 0.51dS$m^{-1}$) was nothing but appearanced Pinus thunbergii Parl., Smilax china L., Quercus dentata Thunb. ex Murray, Quercus serrata Thunb. ex Murray and so on at the level of singular and ideal value. The emerged trees in the high salinity of $EC_{1:5}$0.41dS$m^{-1}$~0.50dS$m^{-1}$ were Albizia kalkora Prain, Melia azedarach L., Paederia scandens (Lour.) Merr. var. scandens These species were trees of tolerance to saline soil. The emerged woody species in all belts were Pinus rigida Mill., Pinus densiflora Siebold & Zucc., Pinus thunbergii Parl., Juniperus rigida Siebold & Zucc. and so on. The woody species with high important value(I.V.) were Pinus densiflora Siebold & Zucc., Pinus thunbergii Parl., Pseudosasa japonica (Siebold & Zucc. ex Steud.) Makino, Smilax china L., Platycarya strobilacea Siebold & Zucc. var. strobilacea for. strobilacea and so on, which can be classified as highly tolerant native trees to salt spray drift.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.199-205
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• 1997

Water quality of streams and ground water from areas of different agricultural practices in the small catchments of the Han River basin was investigated. Water samples were collected from upper, middle and lower reaches of the Han River Basin where three types of agricultural management have been practiced : (1) highland agriculture and livestocks in Daegwanryung area, (2) typical upland and paddy farmings in Dunnae (Jucheon River) and Chuncheon (Soyang River) areas, and (3) intensive farming in the plastic film house in Guri area (Wangsuk stream). Water quality was monitored for EC, pH, COD, TSS, N, rations and anions. Concentrations of N, especially nitrate, and phosphorus in both stream and ground water exceeded the standard water quality criteria in many cases, but those of heavy metals were non-detectable or trace in most cases, except for Wangsuk stream where a high level was detected in a specific sampling time. Chemical criteria such as pH, EC and COD of the stream were suitable for irrigation purpose, but nitrate concentrations in ground water used in the intensive plastic film house were high enough to require a special management consideration. A model on the irrigation water quality incorporating EC and nitrate concentrations was suggested in view of fertilizer management and environmental quality.

• Journal of Embryo Transfer
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• v.32 no.1
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• pp.25-31
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• 2017

This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.3
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• pp.15-25
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• 2019

The study was conducted to compare the chemical properties and microbial activity of soil and the crop productivity by applying homemade liquid fertilizers (LF) used in leading cucumber farms as well as to evaluate the eco-friendly LFs to substitute for a chemical fertilizer. Three homemade LFs, EM, starfish, and native microbes, and a chemical LF were regularly fertigated per three days during the growing season. Chemical LF contained the highest pH, EC (electrical conductivity), and concentrations of T-N, $P_2O_5$, K, Ca, and Mg, while the lowest EC level was observed for EM LF. Soil EC was the highest to the 3.0 dS/m for chemical LF-plots, with lowering soil pH, OM (organic matter), and Mg concentration. Soil chemical properties mostly increased in native microbes LF-plots. However, soil microbial properties were not significantly different among the LF treatment plots. OTU (operational taxonomic units), richness estimator, and diversity index of bacteria and fungi increased in the chemical LF and EM LF based on the pyrosequencing analysis. SPAD and PS II values on the treated-cucumber leaves were seasonally decreased from 32 to 60 days after transplanting, with the rapid decline observed at 45 days after transplanting. Number of leaves and crop height increased in the treatments with EM and native microbes LF. LF treated-cucumber crops were not significantly different for total fresh weight and fruit yield.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.675-685
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• 2022

A comparative analysis was performed on the soil chemical properties of greenhouse or open field where flower crops were grown from 2018 to 2020. The pH of greenhouse soils was kept slightly higher than the optimum range suggested by Rural Development Administration and that of open field soils was maintained within the optimum range for three years. The contents of organic matter (OM) were within the optimum range without significant change every year in both soils. Available phosphate (Av. P₂O₅) of greenhouse soils was the highest at 560 mg/kg in 2018, but it decreased every year and fell within the appropriate range in 2020. The concentration of Av. P₂O₅ in open field soils have fluctuated for three years, not showing a significant difference. Electrical conductivity (EC) of greenhouse soils was higher every year than the standard, 2.0 dS/m, but EC of open field soils remained below the standard. The contents of exchangeable cations were higher than the standard, showing significant differences among the years in greenhouse soils. In open field soils, other cations except exchangeable K⁺ were maintained higher than the optimal level and only Ca²⁺ showed a significant difference among the years. In Pearson correlation matrices, the value of exchangeable Ca²⁺ had a significantly positive correlation with exchangeable Mg²⁺ content at both greenhouse and open field soils. Based on principal component analysis, the soils of greenhouse were distributed within the range of high concentrations of Av. P₂O₅, EC and exchangeable cations, while the soils of open field were characterized by low contents of OM and exchangeable cations. Therefore, it is essential to lower the concentration of exchangeable cations in greenhouse soils. It is common for the soils of open field to have a low OM content, so that organic fertilizers should be more actively applied to the soils in open field.

• Journal of Marine Life Science
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• v.6 no.2
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• pp.73-79
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• 2021

Bisphenol A is a representative endocrine disruptor and continuously detected in aquatic environment due to wide use, resulting in adverse effects on growth, development, and reproduction in diverse organisms as well as human. Structural analogs have been developed to substitute BPA are also suspected to have endocrine disrupting effects. In the present study, the time-dependent expression patterns of ecdysteroid synthesis (nvd, cyp314a1), receptors (EcRA, EcRB, USP, ERR), and downstream signaling pathway - related genes (HR3, E75, Vtg, VtgR) were investigated using quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) in the brackish water flea Diaphanosoma celebensis exposed to Bisphenol analogs (BPs; BPA, BPF, and BPS) for 6, 12, and 24 h. As results, the expression of nvd, cyp314a1, EcRs, USP, ERR and E75 mRNA was upregulated at 6 h exposure to BPF, which is earlier than BPA and BPS (12 h). On the other hand, HR3, E75 and VtgR mRNA levels were elevated at 6 h earlier at BPS and BPF than at BPA (12 h), but Vtg mRNA level was slightly changed within 24 h. These findings suggest that like BPA, BPF and BPS can also modulate the transcription of ecdysteroid pathway - related genes with different mechanisms, and have a potential as endocrine disruptors. This study will provide a better understanding the molecular mode of action of bisphenols on ecdysteroid pathway in the brackish water flea.