• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.361-368
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• 1998

The E2 protein of HCV (hepatitis C virus) is thought to have a potential role in the development of subunit vaccines and diagnostics. To express it by the insect cell/baculovirus expression (Bacu) system, we constructed a recombinant Autographa californica nuclear polyhedrosis virus (AcIL3E2), determined the most appropriate expression conditions in terms of host cell line and culture medium, and characterized the expressed HCV E2 protein. A culture system using Trichoplusia ni BTI-TN5Bl-4 cells and SF 900IISFM medium expressed a relatively high level of HCV E2 protein. It was revealed that its glycosylation properties and subcellular localization were almost the same as the ones in the mammalian cell expression system previously reported, suggesting the recombinant HCV E2 protein derived from our Bacu system can be utilized for development of a subunit vaccine and diagnostics. Interestingly, HCV E2 protein was not degraded at all even at 43 h post-heat shock in the heat shock-induced necrotic cells, probably due to its integration into the microsomal membrane, indicating that heat shock can be employed to purify HCV E2 protein.

• Journal of Aquaculture
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• v.11 no.4
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• pp.557-564
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• 1998

Vitellogenin (VTG) induction in response estradiol-17${\beta}$ ($E_2$) were electrophoretically examined in primary hepatocyte cultures in rainbow trout. The hepatocytes were maintained as monolayers on positively charged dishes for up to 7 days. The viability of hepatocytes on Day 7 in cultures decreased about 20.7% and 23.6% with and without $E_2$, respecitively. The amount of DNA per dish also decreased to 13.7% and 14.0% with and without $E_2$, respectively. There were no differences in viability and DNA content between the control and $E_2$-treated culture. Moreover, the rate of VTG to total protein concentrations reached the maxium level at the addition of $10^{-6}$ M E2, to the incubation medium. However, the higher concentration of $10^{-5}$ M $E_2$ rater depressed the level.

• Progress in Superconductivity and Cryogenics
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• v.24 no.3
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• pp.30-34
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• 2022

Effects of electron beam (EB) irradiation on the mechanical strength of Cu (conducting sheath) and Nb (diffusion barrier) of Cu/Nb/MgB₂ superconducting was investigated. Wire- and tape-type Cu/Nb/MgB₂ samples were irradiated at E-beam energy of 2.5 MeV and 5 mA and a maximum E-beam dose was 5×10¹⁷ e/m². The hardness value of Cu and Nb region was measured by the Vickers micro-hardness method. In the case of the wire sample, the hardness of Cu and Nb increased proportionally as the dose was increased up to 5×10¹⁷ e/m², whereas in the case of the tape sample, the hardness increased up to a dose of 0.5×10¹⁷ e/m², and decreased slightly 5×10¹⁷ e/m². The hardness increase of Cu and Nb is believed to be due to the decrease of the deformability of Cu and Nb due to the defects formed inside the materials by E-beam irradiation.

• Archives of Pharmacal Research
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• v.23 no.4
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• pp.267-282
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• 2000

Cytochrome P45O (CYP) 2E1 catalyzes the metabolism of a wide variety of therapeutic agents, procarcinogens, and low molecular weight solvents. CYP2E1-catalyzed metabolism may cause toxicity or DNA damage through the production of toxic metabolites, oxygen radicals, and lipid peroxidation. CYP2E1 also plays a role in the metabolism of endogenous compounds including fatty acids and ketone bodies. The regulation of CYP2E1 expression is complex, and involves transcriptional, post-transcriptional, translational, and post-translational mechanisms. CYP2E1 is transcriptionally activated in the first few hours after birth. Xenobiotic inducers elevate CYP2E1 protein levels through both increased translational efficiency and stabilization of the protein from degradation, which appears to occur primarily through ubiquitination and proteasomal degradation. CYP2E1 mRNA and protein levels are altered in response to pathophysiologic conditions by hormones including insulin, glucagon, growth hormone, and leptin, and growth factors including epidermal growth factor and hepatocyte growth factor, providing evidence that CYP2E1 expression is under tight homeostatic control.

• Asia pacific journal of information systems
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• v.33 no.3
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• pp.792-811
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• 2023

Recently, Web 3.0 services such as virtual currency, block chain, and NFT are attracting public attention. Users who depended on platforms are moving away from being dependent on service providers and moving to Web 3.0 services. Representative services of Web 3.0 include P2E (Play to Earn) and M2E (Move to Earn). In the case of P2E, various studies have been conducted as it is widely covered in the press and media, but research on M2E is relatively lacking. This study attempts to identify the intention to use M2E by using the expanded technology acceptance model. External factors were selected based on M2E's own characteristics and personal characteristics, a research model was designed, and the proposed hypotheses were verified through factor analysis and goodness of model fit. As a result of the study, it was confirmed that profitability, innovativeness, and self-expression had a positive effect on perceived characteristics, and that perceived usefulness, perceived enjoyment, and social influence had a positive effect on intention to use. Through the research results, practical implications for efficient service operation that meets the needs of users are presented to M2E platform providers.

• Molecules and Cells
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• v.21 no.2
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• pp.294-301
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• 2006

We have previously reported that phosphorylation of eukaryotic elongation factor 2 (eEF2) is related to the differentiation of chick embryonic muscle cells in culture. In the present study, we found that eEF2 phosphorylation declined shortly after induction of differentiation of L6 myoblasts, when the cells prepare for terminal differentiation by withdrawing from the cell cycle. This decrease in phosphorylation was prevented by inhibitors of phosphoinositide 3-kinase (PI3-kinase) that strongly inhibit myoblast differentiation. We hypothesized that PI3-kinase plays an important role in myoblast differentiation by regulating eEF2 phosphorylation in the early stages of differentiation. To test this hypothesis, myoblasts were synchronized at in $G_2/M$ and cultured in fresh differentiation medium (DM) or growth medium (GM). In DM the released cells accumulated in $G_0$/$G_1$ while in GM they progressed to S phase. In addition, cyclin D1 was more rapidly degraded in DM than in GM, and eEF2 phosphorylation decreased more. Inhibitors of PI3-kinase increased eEF2 phosphorylation, but PI3-kinase became more activated when eEF2 phosphorylation declined. These results suggest that the regulation of L6 myoblast differentiation by PI3-kinase is related to eEF2 phosphorylation.

• IMMUNE NETWORK
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• v.11 no.5
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• pp.268-280
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• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.264-267
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• 2003

B2B electronic commerce of various form is gone present and e-Catalog that is used in electronic commerce is important element middle who can express well special quality of corporation's goods and product most effectively from infinite internet space. But, e-Catalog system that is used in electronic commerce is no clear concept and consistent component etc. and is constructing dissimilar system and there is shortcoming that can not use e-Catalog information exchange and transaction thereby. Propose XML(eXtensible Markup Language) in e-Catalog's standard document format to augment interoperability in selfishness species system in treatise that see hereupon, and defined e-Catalog document structure that can process goods information configurationally because using XML Schema. Also, e-Catalog registry system that offer search, registration service using e-Catalog document that is defined by XML design and embody.

• Journal of Korean Society on Water Environment
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• v.39 no.5
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• pp.396-412
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• 2023

Species from the mayfly genus Ephemera (Order Ephemeroptera) was assessed for their physical microhabitat suitability (namely E. strigata, E. separigata, and E. orientalis-sachalinensis). Probability distribution models (Exponential, Normal, Lognormal, Logistic, Weibull, Gamma, Beta, and Gumbel) based on the data collected from 23,957 sampling units of 6,787 sites in Korea from 2010 to 2021 were used. Mode and standard deviation calculated from the best-fitting models to species distribution along a water depth gradient were 265 cm and 159 cm in E. orientalis-sachalinensis; 10 cm and 83 cm in E. strigata; 20 cm and 15 cm in E. separigata, respectively. The current velocity gradient was 22 cm/s and 40 cm/s in E. orientalis-sachalinensis; 60 cm/s and 53 cm/s in E. strigata; 82 cm/s and 25 cm/s in E. separigata, respectively. The mean diameter (phi scale) of substrate grains were -3.6 and 2.2 in E. orientalis-sachalinensis; -7.4 and 1.5 in E. strigata; -5.8 and 0.9 in E. separigata, respectively. Habitat suitability range of E. orientalis-sachalinensis was estimated to be 161~369 cm (water depth), 5~44 cm/s (current velocity), -5.2~-2.0 (mean diameter); 3~34 cm (water depth), 36~94 cm/s (current velocity), -8.1~-6.3 (mean diameter) for E. strigata; 12~32 cm (water depth), 63~96 cm/s (current velocity), -6.3~-5.2 (mean diameter) for E. separigata. In relative comparison, E. orientalis-sachalinensis was estimated to be rheophobic, eurybathophilic, and eurypsephophilic; E. strigata to be euryrheophilic, bathophobic, and lithophilic; E. separigata to be stenomesorheophilic, stenobathophobic, stenolithophilic.

• Korean Journal of Ichthyology
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• v.8 no.1
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• pp.49-55
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• 1996

The growth of total length(TL), total weight(TW) and somatic weight(SW) of rainbow trout, Oncorhynchus mykiss alevin at yolk absorption period was expressed by the Gompertz growth model as $TLt=2.7e^{-1.24{\cdot}e^{-0.11t}}(r^{2}=0.66)$, $TWt=1.8e^{-2.03{\cdot}e^{-0.11t}}(r^{2}=0.66)$ and $SWt=1.8e^{-5.41{\cdot}e^{-0.13t}}(r^{2}=0.83)$ respectively. Yolk length, yolk height and yolk volume of rainbow trout decreased linearly. The relative growth of total weight-total length, somatic weight-total length, yolk length-total length, yolk height-total length, yolk volume-total length, yolk weight-total length, yolk weight-total weight, yolk weight-yolk height and yolk weight-yolk length at yolk absorption period revealed the pattern of yolk absorption in rainbow trout.