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Production of monoclonal antibodies against marine birnavirus (Marine birnavirus (MABV)에 대한 단클론 항체 생산)

  • Kong, Kyoung-Hui;Oh, Myung-Joo;Kim, Wi-Sik
    • Journal of fish pathology
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    • v.33 no.2
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    • pp.171-175
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    • 2020
  • We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.

Fabrication and characterization of glass with E-glass fiber composition by using silica-alumina refused coal ore (사암계 석탄폐석을 활용한 E-glass fiber 조성의 유리 제조 및 특성)

  • Lee, Ji-Sun;Lim, Tae-Young;Lee, Mi-Jai;Hwang, Jonghee;Kim, Jin-Ho;Hyun, Soong-Keun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.4
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    • pp.180-188
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    • 2013
  • The glass of E-glass fiber composition was fabricated by using refused coal ore which is obtained as by-product from Dogye coal mine in Samcheok. We used silica-alumina refused coal ore which has low carbon content relatively, and the amount of refused coal ore has been changed from 0 to 35 % in batch composition. E-glass was fabricated by the melting of mixed batch materials at $1550^{\circ}C$ for 2 hrs with different refused coal ore composition of 0~35 %. We obtained a transparent and clear glass with high visible light transmittance value of 81~84%, thermal expansion coefficient of $5.39{\sim}5.61{\times}10^{-6}/^{\circ}C$ and softening point of $851{\sim}860^{\circ}C$. The glass fiber samples were also obtained through fiberizing equipment at $1150^{\circ}C$, and tested chemical resistance and tensile strength to evaluate the mechanical property as a reinforced glass fiber of composite material. As the result, we identified the properties of E-glass fiber by using refused coal ore are plenty good enough compare to that of normal E-glass without refused coal ore, and confirmed the possibility of refused coal ore as for the raw material of E-glass fiber.

An Empirical Study on the Effects of e-Mail Marketing : A focus on e-Mail Campaign for Credit Card Consumers (이메일 마케팅 성과에 관한 연구: 신용카드 고객을 대상으로 한 캠페인을 중심으로)

  • Shin, Sung-Hoon;Chung, Soo-Yeon;Park, Cheol
    • Information Systems Review
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    • v.11 no.1
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    • pp.49-67
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    • 2009
  • E-mail marketing is the cheapest channel in target marketing. The channel works amazingly well for marketers who know how to use it. The e-mail marketers are able to integrate transactional and behavioral data to improve the targeting content of e-mail marketing campaigns. The cost in e-mail marketing is low and e-mail marketing makes no pollution. But, the e-mail response rate is lower than all the other channels. So, it is very hard for companies to increase their sales volumes, though the companies are ready to execute e-mail marketing campaigns on the side of computer systems. Marketers can send messages easily to target customers compared to other channels. But, the possibility to be read by the customers is low. Normal e-mails are continually devalued by spam mails. This study shows the influence of e-mail marketing to increase sales used by credit cards, on the basis of the real data promoted by A bank, in the Republic of Korea. The analysis on the traits of the respondent can help marketers to target customers. If additional studies on the response prediction model on the basis of traits of potential respondents are done, the targeting method to increase the effectiveness of e-mail marketing will be better structured and organized.

A Design of Mobile e-Book Viewer interface for the Reading Disabled People (독서장애인용 모바일 전자책뷰어 인터페이스 설계)

  • Lee, KyungHee;Kim, TaeEun;Lee, Jongwoo;Lim, Soon-Bum
    • Journal of Korea Multimedia Society
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    • v.16 no.1
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    • pp.100-107
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    • 2013
  • As the eBook market grows fast recently, various eBook viewer solutions such as hardware viewers and software readers came out to the market. We can, however, hardly find mobile eBook interfaces for the reading disabled people who have difficulties in reading for their visual impairment or learning disabilities, or dyslexia. An eBook viewer interfaces for the reading disabled people should be carefully and distinctively designed because the reading disabled people cannot use normal versions of eBook viewer. In this paper, we suggest a eBook viewer interface model to make the reading disabled people read eBooks easily. Depending on the type of the reading disabled people: the full blind, the almost blind, the just learning disabled, our model provides an adaptive interface to make them read eBooks effectively. In addition, unlike the existing simple audio books, we also support annotation systems to make the reading disabled people interact with eBook viewer. To show the effectiveness of our model, we implemented an eBook viewer prototype on an android-based mobile device. We are sure that our model and implementation can make the reading disabled people, who is 10% of all the domestic people, read eBooks effectively.

A Study on Mapping Relations between eBook Contents for Conversion (전자책 문서 변환을 위한 컨텐츠 대응 관계에 관한 연구)

  • 고승규;임순범;김성혁;최윤철
    • The Journal of Society for e-Business Studies
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    • v.8 no.2
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    • pp.99-111
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    • 2003
  • By virtue of diverse advantages derived from digital media, eBook is getting started to use. And many market research agencies have predicted that its market will be greatly expanded soon. But against those expectations, copyright-related problems and the difficulties of its accessing inherited from various eBook content formats become an obstacle to its diffusion. The first problems can be solved by DRM technology. And to solve the second problems, each nation has published its own content standard format. But the domestic standards are useful only the domestic level, they still leave the problems in the national level. The variety of content formats has created a demand for mechanisms that allow the exchange of eBook contents. Therefore we study the mapping relations between eBook contents for conversion. To define the mapping relations, first we extract the mapping both between eBook contents and between normal XML documents. From those mappings, we define seven mapping relations and classify them by cardinality. And we analyze the classified relations, which can be generated by automatic, or not. Using these results, we also classify the eBook content conversion as automatic, semi-automatic, and manual. Besides, we provide the conversion templates for mapping relations for automatic generation of conversion scripts. To show the feasibility of conversion templates, we apply them to the eBook content conversion. Experiment shows that our conversion templates generate the conversion scripts properly. We expected that defined mapping relations and conversion templates can be used not only in eBook content conversion , but also in normal XML document conversion.

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Assessment of the Location of the Peroneus Longus Tendon in the Cuboid Groove Using 3D Isotropic Fast Spin-Echo MRI

  • Lee, Ji Yoon;Choo, Hye Jung;Lee, Sun Joo;Jung, Joon-Yong;Kim, Dong Wook;Baek, Jin Wook;Heo, Young Jin;Gwak, Heui-Chul
    • Investigative Magnetic Resonance Imaging
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    • v.24 no.1
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    • pp.21-29
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    • 2020
  • Purpose: To investigate normal location of the peroneus longus tendon (PL) in the cuboid groove by evaluating it between ankles with no significant abnormality (asymptomatic group) and those with retromalleolar PL dislocation (dislocation group) using three-dimensional isotropic fast spin-echo (3D-FSE) magnetic resonance imaging (MRI) of the ankle. Materials and Methods: Thirty-six and 32 3D-FSE ankle MRI were assigned to the asymptomatic group and the dislocation group, respectively. Using multiplanar reformatted 3D-FSE, qualitative PL location (i.e., outside, overlying, and inside in relation to the cuboid groove), quantitative PL location (i.e., distance between the proximal margins of PL and cuboid groove), and cuboid groove size were measured in lateral, middle, and medial levels of the cuboid groove. Results: In the asymptomatic group, 64%, 42%, and 11%, respectively, had the outside or overlying-located PL in lateral, middle, and medial levels of the cuboid groove and the quantitative location gradually decreased from lateral to medial level. Qualitative and quantitative PL locations were not significantly different between the asymptomatic group and dislocation group. Cuboid groove size showed significant negative correlation with quantitative PL location in both groups. Conclusion: Outside- or overlying-located PL in lateral and middle levels of the cuboid groove would be a normal finding, regardless of PL status at the retromalleolar level.

Orbit Analysis for KOMPSAT-2 During LEOP and Mission Lifetime (아리랑위성 2호 초기운용 및 임무기간 중 궤도 분석)

  • Kim, Hae-Dong;Jung, Ok-Chul;Kim, Eun-Kyou
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.38 no.9
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    • pp.914-924
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    • 2010
  • In this paper, results on the orbit analysis for the KOMPSAT-2 satellite using a real orbit data during the LEOP and normal mission lifetime are presented. In particular, the preparation and performance of an orbit operations during the LEOP is emphasized and the effects of space environments (i.e., Solar activity) on orbit evolutions are investigated comparing to those of the KOMPSAT-1 satellite. The summarized results in this paper would be an important reference to improve the stability and effectiveness of satellite operations during the LEOP and normal mission lifetime in case of LEO satellites such as successors of KOMPSAT-2 (i.e., KOMPSAT-3, KOMPSAT-3A, KOMPSAT-5).

Breakdown Characteristics and Lifetime Estimation of Rubber Insulating Gloves Using Statistical Models

  • Kim, Doo Hyun;Kang, Dong Kyu
    • International Journal of Safety
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    • v.1 no.1
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    • pp.36-42
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    • 2002
  • This paper is aimed at predicting the life of rubber insulating gloves under normal operating stresses from relatively rapid test performed at higher stresses. Specimens of rubber insulating gloves are subject to multiple stress conditions, i.e. combined electrical and thermal stresses. Two modes of electrical stress, step voltage stress and constant voltage stress are used in specimen aging. There are two types of test for electrical stress in this experiment: the one is Breakdown Voltage (BDV) test under step voltage stress and thermal stress and the other is lifetime test under constant voltage stress and temperature stress. The ac breakdown voltage defined as the break-down point of insulation that leakage current excesses a limit value, l0mA in this experiment, is determined. Because the very high variability of aging data requires the application of statistical model, Weibull distribution is used to represent the failure times as the straight line on Weibull probability paper. Weibull parameters are deter-mined by three statistical methods i.e. maximum likelihood method, graphical method and least squares method, which employ SAS package, Weibull probability paper and FORTRAN, respectively. Two chosen models for predicting the life under simultaneous electrical and thermal stresses are inverse power model and exponential model. And the constants of life equation for multistress aging are calculated using numerical method, such as Gauss Jordan method etc.. The completion of life equation enables to estimate the life at normal stress based on the data collected from accelerated aging test. Also the comparison of the calculated lifetimes between the inverse power model and the exponential model is carried out. And the lifetimes calculated by three statistical methods with lower voltage than test voltage are compared. The results obtained from the suggested experimental method are presented and discussed.

A Paleomagnetic Study of Cretaceous Rocks from the Euiseong Area (의성지역에 분포하는 백악기 지층에 대한 고지자기 연구)

  • Doh, Seong-Jae;Kim, Kwang-Ho
    • Economic and Environmental Geology
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    • v.27 no.3
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    • pp.263-279
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    • 1994
  • Paleomagnetic and rock-magnetic data of Cretaceous sedimentary and volcanic rocks from the Euiseong area indicate that the stable components of remanence are carried by single and pseudo-single domain magnetite, with the exception of the Shinyangdong Formation which has been remagnetized. The Hayang Group, except for the remagnetized Shinyangdong Formation, yields the mean characteristic direction of $D/I=22.5^{\circ}/57.2^{\circ}$ (${\alpha}_{95}=4.6^{\circ}$, N=14 sites) and the pole position is $72.0^{\circ}N$, $206.4^{\circ}E$ ($dp/dm=4.9^{\circ}/6.7^{\circ}$). The Yucheon Group shows two polarities and the mean characteristic direction of $D/I=351.2^{\circ}/60.5^{\circ}$ (${\alpha}_{95}=11.2^{\circ}$, N= 19 sites) and the pole position is $81.3^{\circ}N$, $79.0^{\circ}E$ ($dp/dm=13.0^{\circ}/17.0^{\circ}$). The mean directions of both the Hayang and the Yucheon Groups are supported by the McElhinny's fold test at the 99% confidence level and that of the Yucheon Group by a reversal test at the 95% confidence level. A magnetostratigraphic correlation between polarities of the study formations and the Geomagnetic Time Scale indicates that the Hayang Group can be correlated to the Cretaceous Long Normal Superchron (CLNS), and the Yucheon Group to the boundary between the CLNS and the Polarity Chron 33R or later boundaries between normal and reverse polarities. Comparison of the paleopoles from this study with those from the surrounding areas both within the Gyeongsang basin and in the northeastern Asia indicates that the study area was not undergone significant tectonic rotations with respect to the other parts of the Gyeongsang basin and that the Korean Peninsula was the part of the single terrane of the northeastern Asia at least since the CLNS. The Yucheon Group can be divided into four sub-groups based on the paleomagnetic data, suggesting that there were at least four times of volcanic activities in the study area.

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Anti-inflammatory Activity of Extracts from Ultra-Fine Ground Saururus chinensis Leaves in Lipopolysaccharide-Stimulated Raw 264.7 Cells

  • Kim, Dong-Hee;Cho, Jun-Hyo;Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • v.59 no.1
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    • pp.37-43
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    • 2016
  • Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of $100{\mu}g/mL$. The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at $100{\mu}g/mL$ of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin $E_2$, tumor necrosis factor ${\alpha}$, interleukin $1{\beta}$ (IL-$1{\beta}$), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at $50{\mu}g/mL$ of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.