• 한국막학회:학술대회논문집
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• 한국막학회 2003년도 The 4th Korea-Italy Workshop
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• pp.81-85
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• 2003

PU block polymers are constituted by sequences of constitutional units CU which are : a diisocyanate T, a polyol or a polyamine V, an extensor E .Due to the CU different solubility parameters ,hard and soft micro domains are formed;they can aggregate into different phases. A linear PU can be described by two parameters R=T/N and $R_{E}$=(R-1)V/E.A stoichiometric model is proposed which gives the general formula of all the possible PU: X((TV)$_{k}$(TE)$_{n}$)$_{m}$X , where k and m can be calculated from the experimental molecular weights respectively of pre polymer and polymer and n= R-1.Physical properties depend on PU composition and on nature of CU. I.R. analysis is useful for discovering the hydrogen bonds location : DSC gives informations on the presence of the different phases .PALS analysis shows that nano cavities become tighter on increasing n. A series of particular Pus ,obtained from :TDI, a low molecular weight polyol, a cyclic extensor, at different R and RE was prepared and analysed. These PU are amorphous, monophasic and appear to be good candidates for membranes.nes.nes.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.46-46
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• 2021

The first purpose of this study was to evaluate the scavenging capacity (SC) of DPPH and ABTS free radicals for ethanol extract (STR-E) and its active fractions from Sophora tonkinensis root (STR). Four different fractions from STR-E were prepared by using different types of solvents such as chloroform (STR-E-C), ethyl acetate (STR-E-EA), n-butanol (STR-E-B), and water (STR-E-W). STR-E-C showed the highest value of total phenolic content, while STR-E showed the highest value of total flavonoid and terpenoid content. In STR-E and its four fractions, STR-E-EA showed the strongest SC with the lowest SC50 values of the DPPH radicals and ABTS radicals. The second purpose of this study was to evaluate anti-inflammatory activity in the lipopolysaccharide (LPS)-induced RAW 264.7 macrophages treated with STR-E, STR-E-C, and STR-E-EA, respectively. No cytotoxic effect to RAW 264.7 cells was observed at 20 ~ 25 ㎍/ml of STR-E, 10 ㎍/ml of STR-E-C, and 5 ㎍/ml of the STR-E-EA, presenting cell viability values close to that of the untreated control (100%). STR-E, STR-E-C, and STR-E-EA significantly suppressed the LPS-induced nitric oxide (NO) in a dose-dependent manner. Results of reverse-transcription (RT)-qPCR analysis showed that the peak mRNA levels of IL-1β, TNF-α, iNOS, IL-6, and IL-10 were observed in the LPS-stimulated macrophages at 4 h, 2 h, 12 h, 12 h, and 12 h, respectively. The peak mRNA levels of IL-1β, TNF-α, iNOS, and IL-6 were significantly reduced in the LPS-stimulated macrophages co-treated with 20 ㎍/ml and 25 ㎍/ml of STR-E, respectively. In the case of IL-10, its peak mRNA level slightly increased without statistical significance. Compared with the LPS-stimulated macrophages, the peak mRNA levels of IL-1β, TNF-α, iNOS, and IL-6 reduced in the LPS-stimulated macrophages co-treated with 10 ㎍/ml and 20 ㎍/ml of STR-E-C, respectively. In contrast, the peak mRNA level of IL-10 significantly increased at 8 h. Compared with the LPS-stimulated macrophages, the peak mRNA levels of IL-1β, TNF-α, iNOS, and IL-6 reduced in the LPS-stimulated macrophages co-treated with 5 ㎍/ml and 10 ㎍/ml of STR-E-EA, respectively. In contrast, the peak mRNA level of IL-10 increased at 4 h. Taken together, our data indicated that STR-E, STR-E-C, and STR-E-EA activate macrophages to secrete both pro-inflammatory and anti-inflammatory cytokines.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 B
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• pp.855-858
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• 2001

The single transformer appeared the electro-magnetic force must be constructed to support it. it must construct the single transformer to supporting the electro-magnetic force appeared by the cut-off current. the problem is that the electro-magnetic occurs the modification of the single transformer and an serious accident. In the case of the molding-transformer, the part of the molding cast used in the construction occurs the crack, because of the strong force. therefore, in this paper, the molding material used in the molding-transformer is settled by comparing the results from commercial soft ware of F.E.M and a out-equipment circuit.

• 지질공학
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• 제10권3호
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• pp.237-245
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• 2000

본 연구는 터널설계와 관련하여 모량단층의 지질공학적 기본특성을 규명하고 터널설계시 기초자료 제공을 목적으로 수행되었다. 울산광역시 울주군 상북면 덕현리 덕현천 일대에 발달하는 모량단층의 특성을 규명하기 위하여 인공위성 영상을 이용한 선구조 분석, 지표지질조사, 전기비저항 탐사 및 3차원 해석, 시추조사, 광물분석 및 K-Ar절대연령측정 등을 수행하였다. 연구지역내에서 모량단층의 주향은 N20~30$^{\circ}$E, 경사는 70~90NW이고 단층폭은 약 20~60m, 최대 80m, 깊이는 50m이상인 것으로 규명되었다. 단층점토의 K-Ar 절대연령은 5,700$\pm$1.129만년, 1,900$\pm$0.380만년으로 측정되었으며, 수압파쇄시험 결과 최대수평응력의 방향은 N25$^{\circ}$E, N30$^{\circ}$E로 모량단층의 주향과 일치하였다.

• 한국조경학회지
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• 제22권3호
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• pp.79-90
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• 1994

This survey was ocnducted to investgate changes of the plant community structure from 1972 to 1993 in forest of Mt. Kwanak and thirty-five plots that the size of each plot was 10m${\times}$10m(100㎡) were set up and the vegetation analysis was caried out. By the TWINSPAN analysis, the plant community of survey area were divied into Quercus mongolica, Q. mongolica-Pinus densiflora(1), Q. mongolica-P. densiflora(2), Q. acutissima community. The successional trends of the woody species were seemed to be from P. densiflora, Sorbus alnifolia to Q. mongolica in the canopy layer. But the successional trends in the understory and shrub layer were difficult to suppose. The forest vegetation of Mt. Kwanak from 1972 to 1993 was severely decreased in species number and individuals. The sensitive species for the environmental pollution were selected, and the tolerant plants for the acid soil were increased. In comparision with the DBH class distribution from 1972 to 1993, it shows that the ecological succession has stopped. In the analysis of soil characteristics, soil acidification has taken place over last twenty-two years(from pH=5.40 to pH=4.53). The concentration of K+, Ca++ was severly decreased(from K+=0.60m.e./100g to K+=0.06m.e./100g, from Ca++=3.20m.e./100g to Ca++=0.63m.e./100g), which also could seemed to be cause of plant community decline.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.89-97
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• 2015

Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 ± 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40℃, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (k_cat/K_m) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45℃, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176^th residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca²⁺, whichincreased the thermostability of M179.

• 분석과학
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• 제34권4호
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• pp.180-190
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• 2021

This study compared the efficacy of chiral GC and chiral HPLC for the analysis of nicotine. To develop a suitable dispersive liquid-liquid microextraction (DLLME) method, the following parameters were optimized: pH, extraction solvent, dispersive solvent, type and quantity of salt, and laboratory temperature. The validation of the method was carried out by the established HPLC method. The LODs were 0.11 ㎍/mL and 0.17 ㎍/mL for the (S)- and (R)- enantiomers, respectively. The LOQs were 0.30 ㎍/mL and 0.44 ㎍/mL, respectively. The optimal calibration range was between 0.30-18 ㎍/mL and 0.44-4.40 ㎍/mL, respectively, and the correlation coefficient (r²) was 0.9978-0.9996. The intra-day accuracy was 79.9-110.6 %, and the intra-day precision was 1.3-12.0 %. The inter-day accuracy was 87.8-108.0 %, and the inter-day precision was 4.0-12.8 %. E-liquid and biological fluids (urine and saliva) were analyzed using the established method.

• 한국수산과학회지
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• 제29권2호
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• pp.143-149
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• 1996

우리나라 멸치어업에서의 1971~1992년 동안의 22년간 월별 어획량 자료를 시계열 분석하여 어획량을 분석, 예측하였다. 시계열 분석은 다른 생물학적, 해양학적, 사회 경제적인 요소가 없어도 단지 어획량 자료만으로 분석과 예측이 가능하다. 첫 20년간인 1971~1990년 사이의 월별 멸치 어획량 자료를 ARIMA 시계열 모형에 적용시켜 구한 결과는 다음과 같다. 로그 (대수) 변환시켰을 때의 ARIMA 모형: $$(1-0.381B)(1-0.027B^{12}+0.141B^{24})(1-B^1)(1-B^{12})Z_t=(1-0.968B)(1-0.727B^{12})e_t$$, Box-Cox 변환시켰을 때의 ARIMA 모형: $$(1-0.431B)(1-B^{12})Z_t=(1-0.882B^{12})e_t$$, 위의 두 모형중 Box-Cox 변환시킨 것이 로그 (대수) 변환시킨 것보다 예측오차가 적었으며, Box-Cox 변환식은 $Y'=(Y^{0.58}-1)/0.58$ 이었다. 위의 두 모형 중 후자의 모형을 이용하여 1991~1992년 사이의 월별 어획량을 예측하였다. 예측 어획량과 실제 어획량과의 월별 오차범위는 1.0~63.2% (1991년에 1.6~63.2%이고, 1992년에는 1.0~60.4%)였다. 예측 어획량이 각 연도별로 148,201M/T과 148,834M/T인데 비해, 실제 어획량은 170,293M/T, 168,234M/T이었다. 2년 동안의 총어획량에 대한 오차는 12.3%였다. 또한 스펙트럼 분석은 순환변동의 주기가 2.2개월, 6.1개월, 10.2개월, 12개월, 14.7개월에서 상대적으로 큰 성분이 있음을 나타내었다 이 순환변동 성분은 적절한 ARIMA 모형을 결정하는 데도 도움이 된다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.1717-1721
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• 2008

집중호우로 인한 도시 내수배제 능력의 부족으로 월류가 발생하거나 하천의 제방붕괴, 통수능의 부족 등으로 인하여 범람이 발생하는 경우, 재해 예방차원에서 가장 근본적인 것은 침수의 양상을 파악할 수 있어야 한다. 즉, 어느 지역까지 침수지역이 확장될 것인가, 얼마나 빠르게 범람된 흐름이 흘러갈 것인가, 그리고 언제 홍수위가 감소할 것인가를 파악하는 것이 매우 중요하다. 그러므로 국내 도시지역 지형특성에 적합한 침수예측 모형의 개발이 필요하며, 이를 이용하여 다양한 범람 조건을 좀 더 합리적으로 모의할 수 있게 된다. 또한 물리적인 모형과의 비교를 통해 매우 복잡한 지형을 가진 도시침수계산 등이 이루어질 수 있으며, 침수구역에 대한 적절한 예 경보 및 피난대책의 수립이 가능하게 된다. 본 연구에서는 기존의 DEM 기반 침수해석 모형에서 계산시간이 오래 걸린다는 단점을 보완하기 위해 Sub-Grid를 적용한 침수해석 모형을 개발하였다. 하나의 격자는 $e_1$, $e_2$, $e_3$, $e_4$의 표고를 가지는 4개의 보조격자로 구성하였다. $25m{\times}25m$의 Sub-Grid를 갖는 $50m{\times}50m$의 격자에 대한 침수해석 결과와 Sub-Grid가 없이 $25m{\times}25m$ 및 $50m{\times}50m$ 격자로 구성된 대상유역에 대한 침수해석 결과를 비교하였다.

• 한국운동역학회지
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• 제14권1호
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• pp.41-50
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• 2004

The objective of the study is to analyze the myoelectrical activity involved in performing the Swallow movement, a D-level technique, in order to use it as the basic research data in helping train gymnasts in how to perform strength-related techniques. To this end, four national representative athletes who participated in the 2002 Busan Asian Games were selected. The results of the comparison analysis of the individual models are summarized as follows. 1) The results of the E.M.G analysis showed that during the Swallow movement, the myoelectrical activity was detected higher in pectorialis major muscle and bicep brachii muscle than in trapezius muscle and deltoid muscle. 2) The results of the E.M.G analysis showed that during the Swallow movement, the myoelectrical activity was measured high in triceps brachii muscle and palmaris longus muscle, while the myoelectrical activity was recorded low in latissimus dorsi muscle and rectus abdominis muscle. 3) In performing the Swallow in the rings, the mean average (%) was found high in the order of erector spinae, pectorialis major muscle, palmaris longus muscle, triceps brachii muscle, deltoid muscle, latissimus dorsi muscle, and trapezius muscle. All taken together, the athletes showed a difference in the distribution of the muscles during the performance of the Swallow. The muscle that showed a constant distribution among the athletes was pectoralis major muscle, which proves that for a stable performance, it is ideal to increase the myoelectrical activity in pectoralis major muscle.