• Tuberculosis and Respiratory Diseases
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• v.53 no.3
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• pp.275-284
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• 2002

Background : Gemcitabine is a new anti-cancer agent for treating non-small cell lung cancer. Functioning as an antimetabolite, it induces anti-cancer effects by suppressing DNA synthesis after being incorporated into the DNA as a cytosine arabinoside analogue. When Gemcitabine is incorporated into the DNA, the p53 gene may be activated by induction of the DNA defect. However, there are a few studies on the molecular mechanisms of Gemcitabine-induced cell death. This study examined the role of p53 in Gemcitabine-induced cell death. Methods : A549 and NCl-H358 lung cancer cells were used in this study. The cell viability test was done using a MTT assay at Gemcitabine concentrations of 10nM, 100nM, 1uM, 10uM and 100uM. A FACScan analysis with propium iodide staining was used for the cell cycle analysis. Western blot analysis was done to investigate the extent of p53 activation. For the functional knock-out of p53, stable A549-E6 cells and H358-E6 cells were transfected pLXSN-16E6SD which is over expresses the human papilloma virus E6 protein that constantly degrades p53 protein. The functional knock out of p53 was confirmed by Western blot analysis after treatment with a DNA damaging agent, doxorubicine. Results : Gemcitabine exhibited cell toxicity in dose-dependent fashion. The cell cycle analysis resulted in an S phase arrest. Western blot analysis significant p53 activation in time-dependent manner. Gemcitabine-induced cytotoxicity was reduced by 20-30% in the A549-E6 cells and the 30-40% in H358-E6 cells when compared with the A549-neo and H358-neo control cells. Conclusion : Gemcitabine induces an S phase arrest, as expected for the anti-metabolite, and activates the p53 gene, Furthermore, p53 might play an important role in Gemcitabine-induced cell death. Further investigation into the molecular mechanisms on how Gemcitabine activates the p53 gene and its signaling pathway are recommended.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.1151-1157
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• 2015

Human papillomavirus (HPV) is responsible for one of the most frequent sexually transmitted infections. The first phylogenetic analysis was based on a LCR region fragment. Nowadays, 4 variants are known: African (Af-1, Af-2), Asian-American (AA) and European (E). However the existence of sub-lineages of the European variant havs been proposed, specific mutations in the E6 and LCR sequences being possibly related to persistent viral infections. The aim of this study was a phylogenetic study of HPV16 sequences of endocervical samples from C${\acute{o}}$rdoba, in order to detect the circulating lineages and analyze the presence of mutations that could be correlated with malignant disease. The phylogenetic analysis determined that 86% of the samples belonged to the E variant, 7% to AF-1 and the remaining 7% to AF-2. The most frequent mutation in LCR sequences was G7521A, in 80% of the analyzed samples; it affects the binding site of a transcription factor that could contribute to carcinogenesis. In the E6 sequences, the most common mutation was T350G (L83V), detected in 67% of the samples, associated with increased risk of persistent infection. The high detection rate of the European lineage correlated with patterns of human migration. This study emphasizes the importance of recognizing circulating lineages, as well as the detection of mutations associated with high-grade neoplastic lesions that could be correlated to the development of carcinogenic lesions.

• Journal of the Korean Chemical Society
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• v.36 no.5
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• pp.709-719
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• 1992

We synthesized the binuclear tetradentate Schiff base cobalt(II), nickel(II) and copper(II) complexes such as [Co(II)_2(TSBP)(L)_4], [Ni(II)_2(TSBP)(II)_4] and [Cu(II)_2(TSBP)] (TSBP: 3,3',4,4'-tetra(salicylideneimino)-1,1'-biphenyl, L: Py, DMSO and DMF). We identified the binucleated structure of these complexes by elemental analysis, IR-spectrum, UV-visible spectrum, T.G.A. and D.S.C. According to the results for cyclic voltammogram and differential pulse polarogram of 1 mM complexes in nonaqueous solvents included 0.1M TEAP-L (L; Py, DMSO and DMF) as supporting electrolyte, it was found that diffusionally controlled redox processes of four steps through with one electron for binucleated Schiff base Cobalt(II) complex was Co(III)_2 {^\longrightarrow \\_\longleftarrow^e^-}Co(III)Co(II)_2{^\longrightarrow \\_\longleftarrow^e^-}Co(II){^\longrightarrow \\_\longleftarrow^e^-}Co(I){^\longrightarrow \\_\longleftarrow^e^-}Co(I)_2 and two steps with one electron for Nickel(II) and Copper(II) complexes were M(II)_2 {^\longrightarrow \\_\longleftarrow^e^-}M(I)M(I){^\longrightarrow \\_\longleftarrow^e^-}M(I)_2 (M; Ni and Cu) in nonaqueous solvents.

• Mycobiology
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• v.30 no.1
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• pp.1-4
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• 2002

Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP(restriction fragment length polymorphism) of ribosomal DNA, M-13, $(GTG)_5$ and nucleotide sequences of ribosomal ITS(internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and $(GTG)_5$ markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.523-530
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• 2005

In this study, maximum exposure rate at DAW(Dry Active Waste) drum surface which is satisfying regulation limit was calculated for conceptual design of IP(Industrial Package). DAW can be classified as combustible and non-combustible waste and the calculation was conducted for single and mixed radionuclide for each type of waste. In case of combustible waste that mixed radionuclide is uniformly distributed, the maximum exposure rates at drum surface were 3.60E-01, 8.85E-01 and 1.27E+01 mSv/hr for IP Type 1, 2-a and 2-b, respectively. and 3.60E-01, 8.85E-01, 1.27E+01 mSv/hr for single radionuclide(Co-60). In case of non-combustible waste that mixed radionuclide is uniformly distributed, the maximum exposure rates at drum surface were 7.14E-01, 1.83E+00, 2.69E+01 mSv/hr for IP Type 1, 2-a and 2-b, respectively. and 7.13E-01, 1.81E-01, 2.62E+01 mSv/hr for single radionuclide(Co-60). Through this study, the maximum amount of DAW can be transported by IP was suggested as maximum exposure rate at drum surface and the calculation for the other types of waste will be conducted.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.18 no.2
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• pp.162-169
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• 2009

This paper describes a study on dynamic characteristics analysis and dynamic compliance evaluation of a 5-axis multi-tasking machine tool of ram-head type. Structural dynamics analysis and evaluation are necessary to machine tool design and development to secure good machine tool performance against tough and harsh machining conditions. In this study, natural frequencies and corresponding vibration modes of the machine tool structure were analyzed by using both F.E.M. modal analysis and impulse hammer test. Furthermore, dynamic compliance of the machine tool was analyzed by using F.E.M. and also measured by using a hydraulic exciter test. Both the theoretical analysis and experimental test results showed good agreement with each other.

• Journal of Korean Ophthalmic Optics Society
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• v.17 no.1
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• pp.91-97
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• 2012

Purpose: This purpose of study is to investigate the effect of UV-A-blocking or brown-tinted ophthalmic lens against A2E photooxidation which known as one of the etiologies of AMD(Age-related macular degenaration). Methods: The photooxidation of A2E, synthetic product of two molecules of all-trans-retinal and ethanolamine, was induced by the exposure to blue light (420~470 nm, $94mW/cm^2$) for 3 minutes. The inhibitory effect of UVblocking or brown-tinted ophthalmic lens against A2E photooxidation was evaluated by UV absorbance and HPLC analysis of remained A2E after the exposure to blue light. Results: UV-blocking ophthalmic lens could not inhibit A2E photooxidation induced by blue light irradiation. There was no difference in A2E photooxidation in the presence of brown-tinted ophthalmic lens to block 15% of visible ray, however, those lenses blocking 55% or 86% of visible ray showed the inhibitory effect of A2E photooxidation as 9.98% and 16.55%, respectively. By HPLC analysis, the amount of residual A2E which was not blocked by any lens was $199.29{\pm}26.53{\mu}M$, however, the inhibitory effect against A2E photooxidation was shown in the presence of brown-tinted lens. The remained A2Es were $264.58{\pm}31.91{\mu}M$ and $402.93{\pm}28.68{\mu}M$ in brown-tinted lenses of 55% and 86% blocking visible ray, respectively. However, there was no inhibitory effect against A2E photooxidation in the case of UV-blocking lens by HPLC analysis. Conclusions: In this study, brown-tinted ophthalmic lens was confirmed to have the inhibitory effect against the photooxidation of A2E, a causing substance of AMD onset.

• Journal of the Korean Solar Energy Society
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• v.31 no.6
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• pp.95-102
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• 2011

The purpose of study is to estimate heating, cooling load performance and economic efficiency in office building with applied the functional paint. this paint can reduced SHGC(Solar Heat Gain Coefficient) on the glazing surface by coating. In this study, estimated to compared with double glazing, low-e glazing, IP(Insulation Paint) and IPu(Insulation UV-Cut Paint) coating glazing. As a result of this study, 1)heating & cooling load Analysis, SHGC value and U-factor of double glazing is about 0.70 and 3.29($W/m^2K$). low-E glazing is about 0.65 and 2.70($W/m^2K$). Two-side it is about 0.27 and 3.25($W/m^2K$). When compared to double glazing, annual heating & cooling load of low-E glazing, Two-side IPu and IP paint coating glazing is 3,012MWh($124kWh/m^2$), 2,910MWh($120kWh/m^2$), 2,867MWh($118.4kWh/m^2$) and 2,867MWh($118.4kWh/m^2$). It i sreduced to 2.0%, 5.2%, 6.7%, and 6.7% respectively. 2)the estimation of economic efficiency, low-e glazing installed in office building can not recover the investment within a lifetime 40years. but IPu and IP paint, two-side coating in glazing, have a payback period of 13 years respectively.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.05a
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• pp.66-70
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• 2001

The partial discharge testing is widely used in diagnostic measuring technology because it gives low stress to power equipment which is undertaken tests. Therefore it is very useful method compare to previous destructive methods and effective diagnosis method in power system that requires on-line/on-site diagnosis. But partial discharges have very complex characteristics of discharge pattern, so it is required continuous research to development of precise analysis method. In recent, the study of partial discharge is carrying out discover of initial defect of power equipment through condition diagnosis and system development of degradation diagnosis using HFPD(High Frequency Partial Discharge) detection. In this study, simulated system is manufactured and HFPD occurred from those simulator is measured with broad-band antenna in real time, the degradation grade of system is analyzed through produced patterns in simulated target according to the AI/statistics processing.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.05b
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• pp.75-78
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• 2001

Recently, the HFPD measurement testing is widely used in partial discharge measurement of HV machines because HFPD measurement testing receives less influence of external noise and has a merit of good sensitivity. Also HFPD testing is able to offer the judgement standard of degradation level of HV machine and can detect discharge signals in live-line. Therefore it is very useful method compare to previous conventional PD testing method and effective diagnosis method in power transformer that requires live-line diagnosis. But partial discharges have very complex characteristics of discharge pattern so it is required continuous research to development of precise analysis method. In recent, the study of partial discharge is carrying out discover of initial defect of power equipment through condition diagnosis and system development of degradation diagnosis using HFPD(High Frequency Partial Discharge) detection. In this study, simulated transformer is manufactured and HFPD occurred from transformer is measured with broad band antenna in real time, the degradation grade of transformer is analyzed through produced patterns in simulated transformer according to applied voltages.