• Korean Journal of Environment and Ecology
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• v.10 no.2
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• pp.171-183
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• 1997

Diversity, abundance, species distributions and distributions of two strategists (specialist and geceralist) of butterflies were investigated monthly from June 1996 to Oct. 1996 by line transect method in the Chunbul-dong valley from park gate to Yangpok Sanjang. The butterfly faunas of the eight regions in Kwangwon province, Gyunggi proavince and Pusan were also studied by the same method to assess diversity and abundance of butterfly community in the study region. The 18 butterfly species in 7 families were found in this study and the 48 butterfly species in 7 families were distributed in the study area from thes study and the other researches. The 30 butterfly species reported in the other studies were not found in this study and Kaniska canace L. of them would be to decrease in density. In the study area, the common species were Artogeia melete M., Neptis sappho P. and Libythea celtis F.. The representative Korean common butterflies such as Artogeia rapae L., Colias erate E. and Papillio xuthus L. were rare. The PCA(Principal Component Analysis) ordinations indicate that habitat disturbance by human would be major factor for butterfly community and species distribution. Difference was not found in diversity according to habitat disturbance. Abundance, however, was higher in the disturbed region than the undisturbed region. Compared with the other regions, abundance was greatly lower and diversity was slightly lower than that of the other regions. Two strategists exhibited different distribution patterns. Generalists were found more commonly in the disturbed region and widely distributed in both of the disturbed region and the undisturbed region than specialists.

• The Journal of Korean Academy of Prosthodontics
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• v.49 no.1
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• pp.22-28
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• 2011

Purpose: This article attempted to examine how teeth for restoration is made in a clinical practice and utilize it as future educational material of teeth formation and basic data for additional research. Materials and methods: This experiment investigated the models sent to milling center for production of zirconia crowns. After scanned with Lava CAD/CAM System (3M ESPE, Seefeld, Germany), they are measured on 'ImageJ (version 1.32j, National Institutes of Health, USA)' program and compared and analyzed. Convergence angle from mesio-distal surfaces and bucco-lingual surfaces of each teeth are measured. Also, bucco-lingual diameter of the region lowered as much as 0.4 mm from incisal edge in anterior teeth except canines.(This measure is defined as the Peak 0.4) The analysis of data between each group was conducted by Windows SPSS statistic program, and was proved significant on 95% confidence level by independent t-test, one-way ANOVA and multiple analysis (Sheff${\'{e}}$ test). Results: The mean value of convergence angle was $18.67^{\circ}$ It is ranked as molar ($26.70^{\circ}$) > premolar ($16.87^{\circ}$) > anterior teeth ($14.81^{\circ}$) in the order of mesio-distal convergence angle; anterior teeth ($22.32^{\circ}$) > molar ($20.93^{\circ}$) > premolar ($15.41^{\circ}$) in the order of bucco-lingual convergence angle. The mean value of Peak 0.4 was 1.18 mm. Conclusion: Convergence angle of abutment of zirconia all ceramic crown has difference depending on the location in the arch. Due to the nature of production of zirconia all ceramic crown, convergence angle of abutment and line angle finishing degree can have an effect on internal suitability of restoration.

• BMB Reports
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• v.34 no.4
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• pp.334-341
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• 2001

Even though three isotypes of thioredoxins (-f, -m and -h types) have been identified in a variety of plant cells, there are only a few reports on thioredoxin-h that were recently identified. In this study, a cDNA encoding a h-type of thioredoxin was isolated from a cDNA library of Chinese cabbage, and named here CTrx-h. An open reading frame of the gene contained a polypeptide of 133 amino acids with a conserved active center, WCGPC, which appeared in all of the thioredoxin proteins. A deduced amino acid sequence of the CTrx-h showed the highest sequence identity with those of Arabidopsis thioredoxin-h2 (75.2%) and thioredoxin-h5 (46.6%) proteins, but it shared a low sequence homology to other isotypes of plant thioredoxinm and thioredoxin-f. The CTrx-h protein that is expressed in E. coli represented not only an insulin reduction activity, but also electron transferring activity from NADPH to thioredoxin-dependent peroxidase. A genomic Southern blot analysis using the cDNA insert of CTrx-h revealed that the gene consisted of a small multigene family in Chinese cabbage genome. On the contrary to other thioredoxin-h proteins that were widely distributed in most tissues of the plant, the CTrx-h was predominantly expressed in flowers. The expression was very low in other tissues. The data of the Northern blot analysis suggests that the CTrx-h may have other functions in flower development or differentiation, in addition to its defensive role.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.589-596
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• 2008

This study was carried out in order to estimate site indices in un-stocked land by the physico-chemical properties of forest soil and to investigate the distribution of the physico-chemical properties by soil horizons. In Larix leptolepis Gordon stand, 80 sites were selected respectively to A and B horizon in soil. About the analysis method of data there was used stepwise regression analysis. Soil pH and T.N. of the physico-chemical properties turned out to be positively related to the site index. However, O.M. and Avail. $P_2O_5$ in soil were found out negatively related. In the degree of contribution of the variables to site index by each one of the stands, the highly effective variables were Base Sat., C.E.C., Sand, Exch. $Ca^{{+}{+}}$, and Exch. $Mg^{{+}{+}}$ in A horizon, Exch. $Ca^{{+}{+}}$, Base Sat., T.N., Avail. $P_2O_5$, and Clay in B horizon, and Exch. $Ca^{{+}{+}}$ and Base Sat. in both of A and B horizon. In conclusion, these results will provide not only the important criteria for establishment of management forest plan in un-stocked land but also the useful guidance for selecting the suitable sites and trees.

• The Korea Journal of Herbology
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• v.26 no.1
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• pp.41-46
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• 2011

Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin $(PE)E_2$ were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on $PGE_2$ production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.181.2-181.2
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• 2014

A single-layer graphene has been uniformly grown on a Cu surface at elevated temperatures by thermally processing a poly (methyl methacrylate) (PMMA) film in a rapid thermal annealing (RTA) system under vacuum. The detailed chemistry of the transition from solid-state carbon to graphene on the catalytic Cu surface was investigated by performing in-situ residual gas analysis while PMMA/Cu-foil samples being heated, in conjunction with interrupted growth studies to reconstruct ex-situ the heating process. We found that the gas species of mass/charge (m/e) ratio of 15 ($CH_3{^+}$) was mainly originated from the thermal decomposition of PMMA, indicating that the formation of graphene occurs with hydrocarbon molecules vaporized from PMMA, such as methane and/or methyl radicals, as precursors rather than by the direct graphitization of solid-state carbon. We also found that the temperature for dominantly vaporizing hydrocarbon molecules from PMMA and the length of time, the gaseous hydrocarbon atmosphere is maintained, are dependent on both the heating temperature profile and the amount of a solid carbon feedstock. From those results, we strongly suggest that the heating rate and the amount of solid carbon are the dominant factors to determine the crystalline quality of the resulting graphene film. Under optimal growth conditions, the PMMA-derived graphene was found to have a carrier (hole) mobility as high as ${\sim}2,700cm^2V^{-1}s^{-1}$ at room temperature, which is superior to common graphene converted from solid carbon.

• Korean Journal of Environmental Agriculture
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• v.10 no.2
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• pp.113-118
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• 1991

The soil residual amount of herbicides nitralin and napropamide was determined in 6 winter crops field using a chemical assay method. Chemical analysis on soil residue revealed that a relatively high amount of nitralin was detected between 150 and 200 days after treament(DAT). However, regardless of soil types the residue at 220 DAT ranged from 0.053 to 0.141ppm and from 0.134 to 0.308ppm, at the rate of 75 and 150 g a.i./10a, respectively. The residue of napropamide applied at the rate of 75 g a.i./l0a ranged from 0.006 to 0.072ppm at 150 DAT, but not detectable at 220 DAT. When application rate increased to 150 g a.i./10a, napropamide residue was between 0.005 and 0.16ppm at 150 DAT and less than 0.004ppm at 200 DAT, and was not detected at 220 DAT, irrespective of soil types. The results of chemical analysis for the two herbicides showed a similar trend to those obtained from the experiments in agar medium and fields.

• Journal of Biosystems Engineering
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• v.25 no.2
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• pp.97-106
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• 2000

To develop the grain moisture meter using microwave free space transmission technique, a 10.5GHz microwave signal with the power of 11mW generated by an oscillar with a dielectric resonator is transmitted to an isolator and radiated from a transmitting $2{\times}2$ microstrip patch array antenna into the sample holder filled with the 12 to 26%w.b. of Korean Hwawung paddy rice. the microwave signal, attenuated through the grain with moisture, is collected by a receiving $2{\times}2$ microstrip patch array antenna and detected using a Shottky diode with excellent high frequency characteristic. A pair of light and simple microstrip patch array antenna for measurement of grain moisture content is designed and implemented on atenflon substrate with trleative dielectric constant of 2.6 and thickness of 0.54 by using Ensemble ver. 4.02 software. The aperture of microstrip patch arrays is 41 mm width and 24mm high. The characteristics of microstrip patch antenna such as grain. return loss, and bandwidth are 11.35dBi, -38dB and 0.35GHz($50^{\circ}$ at far-field pattern of E and H plane. The width of the sample holder is large enough to cover the signal between the antennas temperature and bulk density respectively. The calibration model for measurement of grain moisture content is proposed to reduce the effects of fluectuations in bulk density and temperature which give serious errors for the measurements . From the results of regression analysis using the statistically analysis method, the moisture content of grain samples (MC(%)) is expressed in terms of the output voltage(v), temperature (t), and bulk density of samples(${\rho}b$)as follows ;$$MC(%)\;=\;(-3.9838{\times}10^{-8}{\times}v^{3}+8.023{\times}10^{-6}{\times}v^{2}-0.0011{\times}v-0.0004{\times}t+0.1706){\frac{1}{{\rho}b}}{\times}100$ Its determination coefficient, standard error of prediction(SEP) and bias were found to be 0.9855, 0.479%w.b. and -0.0.369 %w.b. respectively between measured and predicted moisture contents of the grain samples.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.174-185
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• 2010

Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. To elucidate the functions of a large population of Chinese cabbage (Brassica rapa) genes and to search efficiently for agriculturally useful genes, we have been taking advantage of the full-length cDNA Over-eXpresser (FOX) gene hunting system. With oligo dT column it purify the each mRNA from the flower organs, leaf and stem tissue. And about 120,000 cDNAs from the library were transformed into $\lambda$-pFLCIII-F vector. Of which 115,000 cDNAs from the library were transformed into T-DNA binary vector, pBigs for transformation study. We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. Full-length Chinese cabbage cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Selfed seeds were harvested from transgenic Arabidopsis. We had selected 2,500 transgenic plants by hygromycin antibiotic tolerant test, and obtained a number of transgenic mutants. Each transgenic Arabidopsis was investigated in morphological changes, fertility and leaf colour. As a result, 285 possible morphological mutants were identified. Introduced cDNA was isolated by PCR amplification of the genomic DNA from the transgenic mutants. Sequencing result and BLAST analysis showed that most of the introduced cDNA were complete cDNAs and functional genes. Also, we examined the effect of Bromelain on enhancing resistance to soft rot in transgenic Chinese cabbage 'Osome'. The bromelain gene identified from FOX hunting system was transformed into Chinese cabbage using Agrobacterium methods. Transformants were screened by PCR, then RT-PCR and real time PCR were performed to analyze gene expression of cysteine protease in the T1 and T2 generations. The anti-bacterial activity of bromelain was tested in Chinese cabbages infected with soft rot bacteria. The results showed that the over-expressed bromelain gene from pineapple conferred enhanced resistance to soft rot in Chinese cabbage.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.5
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• pp.47-62
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• 2020

Rice paddy accounts for approximately 52.5% of all farmlands in South Korea, and it is closely related to the water environment. Climate change is expected to affect not only agricultural productivity also the water and the nutrient circulation. Therefore this study was aimed to evaluate changes of nitrogen load from rice paddy considering climate change scenario uncertainty. APEX-Paddy model which reflect rice paddy environment by modifying APEX (Agricultural Policy and Environmental eXtender) model was used. Using the AIMS (APCC Integrated Modeling Solution) offered by the APEC Climate Center, bias correction was conducted for 9 GCMs using non-parametric quantile mapping. Bias corrected climate change scenarios were applied to the APEX-Paddy model. The changes and uncertainty in runoff and nitrogen load were evaluated using multi-model ensemble. Paddy runoff showed a change of 23.1% for RCP4.5 scenario and 45.5% for RCP8.5 scenario compared the 2085s (2071 to 2100) against the base period (1976 to 2005). The nitrogen load was found to be increased as 43.9% for RCP4.5 scenario and 76.0% for RCP8.5 scenario. The uncertainty analysis showed that the annual standard deviation of nitrogen loads increased in the future, and the maximum entropy indicated an increasing tendency. And Duncan's analysis showed significant differences among GCMs as the future progressed. The result of this study seems to be used as a basis for mid- and long-term policies for water resources and water system environment considering climate change.