• BMB Reports
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• v.36 no.5
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• pp.493-498
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• 2003

The scFv antibody towards the Burkholderia pseudomallei exotoxin was previously constructed by phage display and exhibited good specificity towards the exotoxin. We report here the optimization of the scFv expression in an E. coli expression system. Four different E. coli strains (ER2537, TG1, HB2151, and XL1-Blue) were examined for optimal expression of the scFv protein. Two types of carbon source (i.e. 0.2% glucose and 0.2% glycerol) were also tested for their ability to induce the scFv expression. Cells that carried the scFv construct were grown at $30^{\circ}C$ and induced with 0.05 mM IPTG. The expression was then monitored by SDS-PAGE, Western blotting, and indirect ELISA. The Western blot profile showed different levels of the scFv expression among the host strains; XL1-Blue exhibited the highest level of the scFv protein expression. Glycerol at a concentration of 0.2% (v/v) significantly increased the scFv protein expression level when compared to 0.2% (w/v) glucose. Further optimization demonstrated that the scFv protein expression in XL1-Blue was the most optimal with a glycerol concentration as low as 0.05%. However, by indirect ELISA, only the scFv protein that was expressed in 0.2% (v/v) glycerol exhibited high specificity towards the Burkholderia pseudomallei exotoxin.

• Environmental Analysis Health and Toxicology
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• v.24 no.3
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• pp.219-229
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• 2009

The purpose of this study is to evaluate the skin whitening effect of Scirpi rhizama ethanol extract (SREE) in an animal model. For the experiment of the study, three brown guinea pigs weighing about 450 g to 550 g were exposed to ultraviolet-B rays on the backs at 500 $mJ/cm^2$ once a week, three consecutive weeks and the total quantity of light was 1,500 $mJ/cm^2$. The artificial tanning spots were divided into six different groups including normal (N), control (C), vehicle control (VC), positive control (PC), experimental 1 (E1, 1% SREE), experimental 2 (E2, 2% SREE) groups. Then, 30 ${\mu}L$ of SREE was transdermaly applied on the artificial tanning spots twice a day and 5 days a week for 8 weeks. With the result of a gross observation, it was found that the degree of pigmentation became apparently thinner in the group applied with E2, compared to the control or the vehicle control group. The melanin index of E2 group was significant lower than the control or the vehicle control group. In the observation with a light microscope, it was found that the degree of melanin pigmentation and S-100 protein expression considerably decreased in the groups applied with SREE, compared to the control or the vehicle control group. With the numerical analysis of melanin pigmentation and S-100 protein expression by using image-analysis software, it was found that the tendency was coincide with the results of microscopic observation.

• Journal of Distribution Science
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• v.20 no.11
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• pp.61-69
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• 2022

Purpose: Technological developments make the business competition even tighter. Digitalization makes it easier for business people in their efforts to encourage the formation of consumer buying behavior because of the availability of effective and efficient means of delivering information to be used to make purchasing decisions. The purpose of this study was to determine the effect of E-Wom and Website Attractiveness on E-Trust and Purchase Decision of online sales on Instagram with innovation acting as a moderating variable. Research design, data and methodology: This research sample is 170 Instagram users who have made online purchases from the local fashion brand. This research is quantitative explanative with data collection using questionnaires and analysis techniques using the Structural Equation Modeling (SEM) approach using AMOS. Results: The results showed that E-wom had a significant positive effect on Purchase decisions. Web Attractiveness has no significant effect on Purchase Decision. Ewom has a significant positive effect on E-trust. Web Attractiveness has a significant positive effect on E-trust. E-Trust has no significant effect on purchase decisions. Conclusions: Innovation can moderate the effect of E-trust on Purchase decisions. Purchase Decisions can be influenced by E-wom and E-trust, but not Web Attractiveness.

• Animal Bioscience
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• v.35 no.8
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• pp.1223-1234
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• 2022

Objective: The objectives of this study were to evaluate the effects of daily feed intake during the laying period on embryonic myogenic differentiation 1 (MYOD1), myogenic factor 5 (MYF5), and myogenic factor 6 (MYF6) gene expression in genetically fat and lean lines of chickens. Methods: An experiment in a 2×2 factorial design was conducted with two dietary intake levels (100% and 75% of nutrition recommendation) and two broiler chicken lines (fat and lean). Two lines of hens (n = 384 for each line) at 23th week of age were randomly divided into 4 treatments with 12 replicates of 16 birds. The experiment started at 27th week of age (5% egg rate) and ended at 54th week of age. Hatched eggs from the medium laying period were collected. Real time polymerase chain reaction analysis was used to analyse the MYOD1, MYF5, and MYF6 mRNA levels of E7, E9, E11, E13, and E15 body tissues and E17, E19, and E21 chest and thigh muscle samples. Results: The results indicated that there were significant effects of line, dietary intake, and interactions between them on MYOD1, MYF5, and MYF6 gene mRNA expression levels in embryonic tissues. Low daily feed intake did not change the expression trend of MYOD1 mRNA in either line, but changed the peak values, especially in lean line. Low daily feed intake altered the trend in MYF5 mRNA expression level in both lines and apparently delayed its onset. There was no apparent effect of low daily feed intake on the trends of MYF6 mRNA expression levels in either line, but it significantly changed the values on many embryonic days. Conclusion: Maternal nutrient restriction affects myogenesis and is manifested in the expression of embryonic MYOD1, MYF5, and MYF6 genes. Long term selection for fat deposition in broiler chickens changes the pattern and intensity of myogenesis.

• The Journal of Korean Academy of Prosthodontics
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• v.36 no.2
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• pp.203-244
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• 1998

The human mandible is always under the condition of loading by the various forces extorted by the attached muscles. The loading is an important condition of the stomatognathic system. This condition is composed of the direction and amount of forces of the masticatory muscles, which are controlled by the neuromuscular system, and always influenced by the movement of both opening and closing. Mandible is a strong foundation for the teeth or various prostheses, nevetheless it is a elastic body which accompanies deformation by the external forces on it. The elastic properties of the mandible is influenced by the various procedures such as conventional restorative treatments, osseointegrated implant treatments, reconstructive surgical procedures and so forth. Among the treatments the osseointegrated implant has no periodontal ligaments, which exist around the natural teeth to allow physiologic mobility in the alveolar socket. And so around the osseointegrated implant, there is almost no damping effect during the transmission of occlusal stress and displacements. If the osseointegrated implants are connected by the superstructure for the stabilization and effective distribution of occlusal stresses, the elastic properties of mandible is restricted according to the extent of 'splinting' by the superstructure and implants. To investigate the change of elastic behaviour of the mandible which has osseointegrated implant prosthesis of various numbers of implant installment and span of superstructre, a three dimensional finite element model was developed and analyzed with conditions mentioned above. The conclusions are as follows : 1. The displacements are primarily developed at the area of muscle attachment and distributed all around the mandible according to the various properties of bone. 2. The segmentation in the superstructure has few influence on the distribution of stress and displacement. 3. In the load case of ICP, the concentration of tensional stress was observed at the anterior portion of the ramus($9.22E+6N/m^2$) and at the lingual portion of the symphysis menti($8.36E+6N/m^2$). 4. In the load case of INC, the concentration of tensional stress was observed at the anterior portion of the ramus($9.90E+6N/m^2$) and the concentration of tensional stress was observed at the lingual portion of the symphysis menti($2.38E+6N/m^2$)). 5. In the load case of UTCP, the relatively high concentration of tensional stress($3.66E+7N/m^2$) was observed at the internal surface of the condylar neck.

• Archives of Pharmacal Research
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• v.27 no.2
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• pp.225-231
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• 2004

This study was designed to investigate the effect of Trolox, a hydrophilic analogue of vitamin E, on the alteration of vasoregulatory gene expression during hepatic ischemia and reperfusion (I/R). Rats were subjected to 60 min of hepatic ischemia in vivo. The rats were treated intravenously with Trolox (2.5 mg/kg) or the vehicle as a control 5 min before reperfusion. Liver samples were obtained 5 h after reperfusion for a RT-PCR analysis on the mRNA for the genes of interest. These mRNA peptides are endothelin-1 (ET -1), potent vasoconstrictor peptide, its receptor $ET_A$ and $ET_B$, vasodilator endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), heme oxygenase-1 (HO-1), tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and cyclooxygenase-2 (COX-2). It was seen that serum alanine aminotransferase and lipid peroxi-dation levels were markedly increased after I/R and Trolox significantly suppressed this increase. In contrast, the glutathione concentration decreased in the I/R group, and this decrease was inhibited by Trolox. ET-1 mRNA expression was increased by I/R, an increase which was prevented by Trolox. The mRNA levels for $ET_A$ receptor was significantly decreased, whereas ET$_{B}$ receptor transcript increased in the I/R group. The increase in $ET_A$ was prevented by Trolox. The mRNA levels for iNOS and HO-1 significantly increased in the I/R group and Trolox attenuated this increase. There were no significant differences in eNOS mRNA expression among any of the experimental groups. The mRNA levels for COX-2 and TNF-$\alpha$ significantly increased in I/R group and Trolox also attenuated this increase. Our findings suggest that I/R induces an imbalanced hepatic vasoregulatory gene expression and Trolox ameliorates this change through its free radical scavenging activity.y.

• 한국해양학회지
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• v.22 no.2
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• pp.71-79
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• 1987

We studied the annual variation of water temperatures in the upper 200m off southeast coast of Korea by means of harmonic analysis of the temperatures at 10 standard depths of 51 stations. The distributions of the mean temperature in coastal zone are almost parallel to the coast, whereas those in offshore are almost zonal. With an increase of depth, the annual amplitude decreases nearly exponentially and the annual phase increases nearly linearly. The average e-folding depth for the annual amplitudes is 65m, vertical change of the annual phase is 180 per 200m, and the vertical eddy conductivity is $4\textrm{cm}^2/sec$. Annual variations of temperatures in the coastal zone of a few tens miles from the coast are considerably different from those in offshore area. The e-folding depths in coastal zone are shallower than those in offshore region, and the changes of phase with depths in coastal zone are smaller than those in offshore region.

• Carbon letters
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• v.12 no.3
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• pp.171-176
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• 2011

Polyacrylonitrile-based carbon nanofibers (CNFs) containing Ti and Mn were prepared by electrospinning. The effect of metal content on the hydrogen storage capacity of the nanofibers was evaluated. The nanofibers containing Ti and Mn exhibited maximum hydrogen adsorption capacities of 1.6 and 1.1 wt%, respectively, at 303 K and 9 MPa. Toward the development of an improved hydrogen storage system, the optimum conditions for the production of metalized CNFs were investigated by characterizing the specific surface areas, pore volumes, sizes, and shapes of the fibers. According to the results of Brunauer-Emmett-Teller analysis, the activation of the CNFs using potassium hydroxide resulted in a large pore volume and specific surface area in the samples. This is attributable to the optimized pore structure of the metal-containing polyacrylonitrile-based electrospun CNFs, which may provide better sites for hydrogen adsorption than do current adsorbates.

• Journal of the Korean Society of Clothing and Textiles
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• v.22 no.1
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• pp.18-28
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• 1998

The objective of the study was to observe the difference of moving function of lower-limb in relation to the length 8t silouutte of tight skirt. Four types of tight skirts (2 lengths$\times$2 silhouettes) were made for the experiment. The surface E.M.S in four different locations of leg muscles (Rectos femoris, Semitendinosus, Tibalis anterior, Gastrocnemius) were recorded. The sensory test to decide how to be fatigued after longtime wearing of skirt were examined two times per a day. The fatigue sensory test was scored a Likert-type scale (1= no fatigue, 5=heavy fatigue). Data were analyzed by the repeated ANOVA ann Duncan's multiple range test with use of SAS Package. The main results of this study were as follows: 1. As a result of analysis of E.M.S., in case of walking on the floor there was significant difference in the moving function according to length of skirt and in case of stepping there was significant differnce in three ways (length silhouette, length, silhouette). 2. From the record of walking the step-length, stride-length, step-width were found affected by garments, but foot-angle was not affected. The moving function of slim type was lower than that of semi type and that of ankle-length skirt was lower than that of knee-length skirt. 3. The results of the sensory test agreed with that of E.M.G and Footprints.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.8
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• pp.96-103
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• 2009

The roll forming process is commonly used for the conventional 'Fe' metal products such as a furniture drawer guide or an up-down slide guide. Recently its applications are variously expanded to the sanitary facilities or electronic devices. It is essentially required the cleanness for the high technology application and any corrosion or rust are not allowed. Therefore, in those applications the stainless steel materials are strongly demanded as the substitution of 'Fe' steel. However the mechanical properties of stainless steel are not suitable for forming process compared with those of 'Fe' steel. Up to now, the conventional F.E.M.(Finite Element Method) has been used to analyze and design the roll forming process. The purpose of this research is to obtain the proper production process and the shape of rolls to manufacture the high precision slide rails made of stainless steel material. The commercial program, SHARPE-RF, is used to analyze the entire roll forming process. The results show that the rolling process and the roll design by F.E.M. are useful from the good agreement between the shapes of products estimated by F.E.M. and those of the actual products.