• Journal of the Korean Society of Hazard Mitigation
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• v.3 no.4 s.11
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• pp.103-109
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• 2003

A numerical analysis of cable-stayed bridge is conducted to determine optimum longitudinal modulus of elasticity which represents the boundary condition between the tower and main girder. The effect of longitudinal modulus of elasticity is investigated for different loading condition (live load, wind load, seismic load), respectively. There are significant changes in the member forces as variations of longitudinal modulus of elasticity, such as, $k_h$=e=100tonf/m/bearing (live load), $k_h$=e=1000tonf/m/bearing (seismic load), However, the wind loads do not affect member forces. The optimum longitudinal modulus of elasticity is determined from considering minimum member forces in the numerical analysis results.

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.456-459
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• 1992

The genes of Halohacteria. gvpD and gvpE. take part in formation of gas vesicle. These mRNA cause a lot of experimental prohlems due to its eharacteristic instahility in the analysis of transcripts. This study allowed easy cloning and sequencing of RNA hy substituting a stable complementary DNA for the mRNA of the genes for an analysis. The weak 111 RNA was reverse transcribed to DNA using reverse transcriptase. and was amplified using PCR method. The transcripts confirmed in this ~,tudy have not heen round in the northern hybridization covering almost all ranges of ORF of the gene. gvpD.

• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.121-128
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• 2022

This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC₅₀ value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 ㎍/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 ㎍/mL), and Prunella vulgaris var. lilacina Nakai (33.3 ㎍/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 ㎍/mL), E. ciliata (25.9 ㎍/mL), and M. dianthera (27.6 ㎍/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 µM Fe(II)/g of extract), E. ciliata (2836.2 µM Fe(II)/g of extract), and M. dianthera (2754.4 µM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

• The Korean Journal of Zoology
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• v.31 no.1
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• pp.56-61
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• 1988

The taxonomic status of Moroco Jagowskii and M oxycephalus a pair of sibling species inhabiting in Korean fresh waters, has been unclear up to date. Recently a sympatric area of these species was found (Kang, 1987). The purpose of this study was to clarify their specific status by analysing specimens collected from the sympatric area of these species. Isozyme analysis and morphometric comparison were performed. Among 26 loci screened 6 loci (Aco, Est-2, E-X, Gk-3, Ipo, Me) showed fixed allelic difference between them and these loci could be used as genetic markers to distinguish them. Isozyme analysis indicates that no hybridization occurs and therefore it is assumed that isolating mechanism is completed and they are distinct species. The mean number of scales above lateral line (SAL) of M Jagowskii and M oxycephalus at sympatric area was 24.93 $\pm$1.95 and 17.33$\pm$0.72 respectively, and it seems as the result of character displacement. A finer microhabitat segregation between them is noticed. M oxycephalus is found along the effluent streams whereas M lagowskii is distributed mostly in the main stream at sympatric area.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.501-504
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• 2008

A concrete carbonation model has been constructed that takes account of the diffusion of carbon dioxide through a coating and reaction with calcium hydroxide, and this model has been validated by an accelerated carbonation experiment. (1) By using values for the coatings calculated on the basis of a diffusion.permeation theory as input data for the analysis of diffusion.reaction carbonation in an unsteady state, the effect of the coatings in reducing carbonation can be represented with high accuracy. (2) Through a sensitivity analysis of the diffusion.reaction carbonation model and the experimental results, we found that the diffusion coefficient of calcium hydroxide shows a high interrelationship at 1e-12($m^2/s$). The reaction rate constant for carbonation shows a high interrelationship at 5e-5($m^3/mol/s$).

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.457-462
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• 2014

Prostaglandin (PG) $E_2$ is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, $PGE_2$ has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular $PGE_2$ levels in HaCaT cells and inhibited 15-PGDH ($ED_{50}$ : $168.4{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$ : $250.0{\mu}g/mL$). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE ($168.4{\mu}g/mL$) was comparable to that of TGF-${\beta}1$ (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of $PGE_2$ level dependent diseases.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.286-290
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• 2006

A genomic library of Streptococcus vestibularis ATCC49124 was constructed in an E. coli plasmid vector, and the urease-positive transformants harboring the urease gene cluster were isolated on Christensen-urea agar plates. The minimal DNA region required for urease activity was located in a 5.6 kb DNA fragment, and a DNA sequence analysis revealed the presence of a partial ureI gene and seven complete open reading frames, corresponding to ureA, B, C, E, F, G, and D, respectively. The nucleotide sequence over the entire ure gene cluster and 3'-end flanking region of S. vestibularis was up to 95% identical to that of S. salivarius, another closely related oral bacterium, and S. thermophilus, isolated from dairy products. The predicted amino acid sequences for the structural peptides were 98-100% identical to the corresponding peptides in S. salivarius and S. thermophilus, respectively, whereas those for the accessory proteins were 96-100% identical. The recombinant E. coli strain containing the S. vestibularis ure gene cluster expressed a high level of the functional urease holoenzyme when grown in a medium supplemented with 1 mM nickel chloride. The enzyme was purified over 49-fold by using DEAE-Sepharose FF, Superdex HR 200, and Mono-Q HR 5/5 column chromatography. The specific activity of the purified enzyme was 2,019 U/mg, and the Michaelis constant ($K_{m}$) of the enzyme was estimated to be 1.4 mM urea. A Superose 6HR gel filtration chromatography study demonstrated that the native molecular weight was about 196 kDa.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.258-267
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• 2002

A flux determination methodology has been built which enables to develop constrained stoichiometric relationships and metabolic balances. The analysis differs from those developed for anaerobic growth conditions in that cell mass formation is a significant sink for carbon. When combined with experimental measurements, a determined system of equations results yielded tricarboxylic acid (TCA) cycle and glycolytic fluxes. The methodology was implemented to determine the fluxes of E. coli B/r and K12, and it was found that as the growth rate in a glucose minimal medium increased, the cells became increasing glycolytic and the TCA fluxes either leveled off or declined. The pattern identified for the TCA fluxes corresponded to ${\alpha}$-ketoglutarate dehydrogenase's induction-repression pattern, thereby suggesting that the induction-repression of the enzyme could result in significant flux changes. When the minimum flux solution was contrasted to the glycolytic and TCA fluxes determined, two observations were made. First, the minimum flux could provide the cell's biosynthetic ATP requirements. Second, at a high growth rate in a glucose medium, the excess glycolytic flux exceeded that of the TCA cycle, which appeared to more closely match the biosynthetic needs.

• Journal of Environmental Health Sciences
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• v.29 no.3
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• pp.35-42
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• 2003

Nitric Oxide(NO) is produced in many organs of the body, including the lung and airways, and it is detectable in the exhaled air. The measurement of exhaled NO(eNO) provides a simple non-invasive means for measuring airway inflammation, such as asthma. We measured eNO among adult male workers to examine the distribution of eNO in healthy people and to find factors affecting eNO. We measured eNO in a sample of 921 adult workers who also performed lung function test and skin prick test. Exhaled NO was measured in a sitting posture without using a nose clip and NO free gas. NO was measured at three expiratory rates(l8; 42; 71 $m\ell$/sec) and the flow rate of 71 $m\ell$/sec was used in analysis. The average eNO concentration was 5.29 $\pm$ 2.98 ppb. The level increased with age but not significantly(P=0.0529). Exhaled NO showed positive relations to the height(P=0.0001), pollen 1 (P=0.0124), asthma history(P=0.0212), allergic rhinitis symptom(P=0.0302). Exhaled NO Concentration of smokers( 4.62 ppb) was significantly lower than that of nonsmokers(5.99 ppb; P<0.0001).

• Aerospace Engineering and Technology
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• v.10 no.2
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• pp.82-86
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• 2011

This paper presents an RF Electric field assessment at 2.290 [GHz] inside launcher PROTON M/Breeze M long fairings, produced by COMS TM. COMS intentional RE due to TM ON under fairing has been assessed considering PROTON M / BREEZE M fairing characteristics. As fairings of all launchers present similar sizes, a similar E-field level under fairing is expected for the other launchers. As a result, the RE level under faring is compliant with COMS RS limits, but is not compliant with RS requirements of the launcher PROTON M/Breeze M.