Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant activity of ten Lamiaceae plant seed extracts

10종 꿀풀과(Lamiaceae) 식물 종자 추출물의 항산화 활성

  • Received : 2022.04.23
  • Accepted : 2022.06.14
  • Published : 2022.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC50 value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 ㎍/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 ㎍/mL), and Prunella vulgaris var. lilacina Nakai (33.3 ㎍/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 ㎍/mL), E. ciliata (25.9 ㎍/mL), and M. dianthera (27.6 ㎍/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 µM Fe(II)/g of extract), E. ciliata (2836.2 µM Fe(II)/g of extract), and M. dianthera (2754.4 µM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

본 연구는 꿀풀과 식물 종자 추출물의 항산화 활성 평가를 통해 식물 유래 천연 항산화제를 탐색하기 위해 진행되었다. 충실률 90% 이상이고 발아율이 50% 이상인 종자를 대상으로 하였다. 총 페놀성 화합물 함량은 속단(6.2 mg GAEs/g seed), 향유(4.5 mg GAEs/g seed) 순으로 높게 나타났다. 총 플라보노이드 함량은 향유(1.0 mg QEs/g seed), 속단(0.6 mg QEs/g seed) 순으로 높게 나타났다. DPPH 라디칼 소거활성은 종자 추출물의 EC50 값을 기준으로 향유(27.5 ㎍/mL), 쥐깨풀(29.2 ㎍/mL), 꿀풀(33.3 ㎍/mL) 순으로 높게 나타났다. ABTS 라디칼 소거활성은 꿀풀(25.6 ㎍/mL), 향유(25.9 ㎍/mL), 쥐깨풀(27.6 ㎍/mL)순으로 높게 나타났다. FRAP 평가는 꿀풀(2910.4 μM Fe(II)/g extract), 향유(2836.2 μM Fe(II)/g extract), 쥐깨풀(2754.4 μM Fe(II)/g extract) 순으로 높게 나타났다. 항산화 활성을 기반으로 한 군집분석에 따라 10종은 항산화 활성이 낮은 group 1에서 활성이 높은 group 3까지 3개의 group으로 분류하였으며, 향유, 쥐깨풀, 꿀풀 종자는 group 3에 속하였다.

Keywords

Acknowledgement

This study was carried out with the support of R&D Program for Forest Science Technology (Project No. 2021400B10-2225-CA02) provided by Korea Forest Service (Korea Forestry Promotion Institute).

References

  1. Kehrer JP, Klotz LO (2015) Free radicals and related reactive species as mediators of tissue injury and disease: implications for health. Crit Rev Toxicol 45(9):765-798. doi: 10.3109/10408444.2015.1074159
  2. Forman HJ, Davies KJ, Ursini F (2014) How do nutritional antioxidants really work: nucleophilic tone and para-hormesis versus free radical scavenging in vivo. Free Radical Bio Med 66:24-35. doi: 10.1016/j.freeradbiomed.2013.05.045
  3. Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10):7313-7352. doi:10.3390/molecules15107313
  4. Erdemoglu N, Turan NN, Cakoco I, Sener B, Aydon A (2006) Antioxidant activities of some Lamiaceae plant extracts. Phytotherapy Research. Phytother Res 20(1): 9-13. doi: 10.1002/ptr.1816
  5. Carovic-StanKo K, Petek, M, Grdisa M, Pintar J, Bedekovic D, Herak Custic M, Satovic Z (2016) Medicinal plants of the family Lamiaceae as functional foods-a review. Czech J Food Sci 34(5): 377-390. doi:10.17221/504/2015-CJFS
  6. Korean National Arboretum (2022) Korea Biodiversity Information System. http://www.nature.go.kr. Assessed 25 January 2022
  7. Korean National Arboretum (2017) Ethnobotany in Korea: The traditional knowledge and use of indigenous plants. Korean National Arboretum Pocheon-si, Gyeonggi-do, Korea, pp 7
  8. Uritu CM, Mihai CT, Stanciu GD, Dodi G, Alexa-Stratulat T, Luca A, Leon-Constantin M, Stefanescu R, Bild V, Melnic S, Tamba BI (2018) Medicinal plants of the family Lamiaceae in pain therapy: A review. Pain Res Manag 2018. doi: 10.1155/2018/7801543
  9. Kim JW, Hong JH (2021) Physicochemical properties and physiological activities of Agastache rugosa extracts. Korean J Food Preserv 28(1): 88-98. doi: 10.11002/kjfp.2021.28.1.88
  10. Lee JH, Yim DS, Choi SS (2021) Antibacterial Activity and Anti-inflammatory Effect of Methanol Extracts of Saliva miltiorrhiza Against Oral Pathogenic Bacteria. Korean J Pharmacogn, 52(1): 41-48. doi:10.22889/KJP.2021.52.1.41
  11. Krishnaiah D, Sarbatly R, Nithyanandam R (2011) A review of the antioxidant potential of medicinal plant species. Food Bioprod Process, 89(3): 217-233. doi: 10.1016/j.fbp.2010.04.008
  12. Song YJ, Chang JP, Yoo JH (2016) Antioxidant Activities of Water Extracts from Different Parts of Lycopus lucidus Turcz. Ex Benth. Kor J Herbol 31(6): 21-28. doi: 10.6116/kjh.2016.31.6.21
  13. Seo JK, Kang MJ, Shin JH, Lee SJ, Jeong HG, Sung NJ, Chung YC (2010) Antibacterial and Antioxidant Activities of Solvent Extracts from Different Parts of Hagocho (Prunella vulgaris). J Korean Soc Food Sci Nutr 39(10): 1425-1432. doi: 10.3746/jkfn.2010.39.10.1425
  14. Kim JH, Im JH, Park CY, Lee DH, Lee MH, Jung YH, Park CH, Na CS (2021) Antioxidant and α-glucosidase inhibitory effects of Pethorum chinense Purch seed extracts. Korean J Food Preserv 28(3): 384-390. doi: 10.11002/kjfp.2021.28.3.384
  15. Kim JH, Lee DH, Lee MH, Jung YH, Park CH, Lee HH, Na CS (2021) Antioxidant Activity of Asteraceae Plant Seed Extracts. J Life Sci 31(6): 543-549. doi: 10.5352/JLS.2021.31.6.543
  16. Royal Botanic Gardens, Kew (2017) Seed information database (SID), ver. 7.1. Assessed 25 January 2022
  17. Davies R, Di Sacco A, Newton R (2015) Germination testing: procedures and evaluation. Technical Information Sheet_13a. Royal Botanic Gardens, KewDezfuli PM, Sharif-Zadeh F, Janmohammadi M (2008) Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN J Agric Biol Sci 3(3): 22-25
  18. Dezfuli PM, Sharif-Zadeh F, Janmohammadi M (2008) Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN J Agric Biol Sci 3(3): 22-25
  19. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Method Enzymol 299: 152-178. doi:10.1016/S0076-6879(99)99017-1
  20. Christ B, Muller KH (1960) Zur serienmassigen Bestimmung des Gehaltes an Flavonol-Derivaten in Drogen. Archiv der Pharmazie. 293(65): 1033-1042 https://doi.org/10.1002/ardp.19602931202
  21. Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181(4617): 1199-1200 https://doi.org/10.1038/1811199a0
  22. Miller NJ, Rice-Evans C, Davies M, Gopinathan V, Milner A (1993) A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84: 407-412 https://doi.org/10.1042/cs0840407
  23. Molina-Diaz A, Ortega-Carmona I, Pascual-Reguera MI (1998) Indirect spectrophotometric determination of ascorbic acid with ferrozine by flow-injection analysis. Talanta 47: 531-536. doi: 10.1016/S0039-9140(98)00085-X
  24. Kim GT, Um TW (1995) A Study for the utilization of wild herbaceous species-effects of gibberelic acid treatment on seed germination. Korean J Environ Ecol 9: 56-61
  25. Jo HJ, Chung KH, Yoon JA, Song BC, An JH (2014) Free radical-scavenging activities of amaranth (Amaranthus spp. L.) seed extracts. Food Eng Prog 18(2): 116-123. doi: 10.13050/foodengprog.2014.18.2.116
  26. Elfalleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A (2012) Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. J Med Plants Res 6(32): 4724-4730. doi: 10.5897/JMPR11.995
  27. Cho HD, Kang WS, Kim DH, Ku JJ, Seo KI (2019) Comparison of biological activity between Stellaria aquatica seed extracts. Korean J Food Preserv 26(2): 228-237. doi: 10.11002/kjfp.2019.26.2.228
  28. Arnao MB (2000) Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practical case. Trends Food Sci Tech 11(11): 419-421. doi: 10.1016/S0924-2244(01)00027-9
  29. Orak H, KARAMAC M, ORAK A, AMAROWICZ R, JANIAK M (2018) Phenolics content and antioxidant capacity of mung bean (Vigna radiata L.) Seed. YYU J Agric Sci 28: 199-207
  30. Zhou K, Wang H, Mei W, Li X, Luo Y, Dai H (2011) Antioxidant activity of papaya seed extracts. Molecules 16(8): 6179-6192. doi:10.3390/molecules16086179
  31. Soong YY, Barlow PJ (2004) Antioxidant activity and phenolic content of selected fruit seeds. Food chem 88(3): 411-417. doi: 10.1016/j.foodchem.2004.02.003
  32. Lee SE, Sung JS, Jang IB, Kim GS, Ahn TJ, Han HS, Kim JE, Kim YO, Park CB, Cha SW, Ahn YS, Park HK, Bang JK, Seong NS (2008) Investigation on antioxidant activity in plant resources. Korean J Medicinal Crop Sci 16(5): 356-370
  33. Ma J, Xu RR, Lu Y, Ren DF, Lu J (2018) Composition, antimicrobial and antioxidant activity of supercritical fluid extract of Elsholtzia ciliata. J Essent Oil Bear Pl 21(2): 556-562. doi: 10.1080/0972060X.2017.1409657
  34. Pudziuvelyte L, Liaudanskas M, Jekabsone A, Sadauskiene I, Bernatoniene J (2020) Elsholtzia ciliata (thunb.) hyl. extracts from different plant parts: phenolic composition, antioxidant, and anti- inflammatory activities. Molecules 25(5): 1153. doi: 10.3390/molecules25051153
  35. Kanyal J, Prakash OM, Kumar R, Rawat DS, Pant AK (2019) Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim: Chemical compositions, biochemical assay, in vitro antioxidant and anti-inflammatory activity of chloroform extract. Int J Chem Stud 7(4): 2807-2813
  36. Lee EJ, Kwon YJ, Noh JE, Lee JE, Lee SH, Kim JK, Kim KS, Choi YH, Kwon JH (2005) Optimization of Microwave-Assisted Process for Extraction of Effective Components from Mosla dinthera M. Korean J Food Preserv 12(6): 617-623
  37. Hwang YJ, Lee EJ, Kim HR, Hwang KA (2013) In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var. lilacina. BMC Complem Altern M 13(1): 1-9 https://doi.org/10.1186/1472-6882-13-1
  38. Lee SA, Lee DH, Baek, JW, Jung EB, Baek JY, Lee IK Jang TS, Kang KS, Kim KH (2017) In vitro assessment of selected Korean plants for antioxidant and antiacetylcholinesterase activities. Pharm Biol 55(1): 2205-2210. doi: 10.1080/13880209.2017.1397179
  39. Park DS, Park MY, Chon SM, Lee JY, Lee YM, Jang HH, Hwang KA, Kim JH (2011) Antioxidant Activity of Different Solvent Fractions from Prunella vulgaris var. lilacina. Korean J Medicinal Crop Sci 19(6): 484-490. doi: 10.7783/KJMCS.2011.19.6.484