• BMB Reports
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• v.38 no.1
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• pp.97-103
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• 2005

Under the condition of expression of $\lambda$ P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the $\lambda$ P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the $\lambda$ P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of $\lambda$ P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.

• Proceedings of the Korean Society of Computer Information Conference
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• 2015.07a
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• pp.208-210
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• 2015

웹 기반의 스마트 교육 서비스 환경에서 스마트 스크린 단말들 간에 혹은 스마트 스크린 단말과 사용자간의 연결성 및 사용자 경험 증대를 위해서는 직관적이고 손쉬운 사용자 인터페이스 기술 등이 필요하다. 본 논문에서 제안된 방법은 단말에 내장된 웹 브라우저위로 사용자의 손가락 제스쳐 인터랙션 등을 통해 웹소켓 기반의 스마트 스크린 단말간 콘텐츠 제어 서비스를 가능하게 한다.

• Smart Media Journal
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• v.11 no.6
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• pp.51-63
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• 2022

Metaverse provides a simulated environment where a large number of users can participate in various activities. In order for Metaverse to be sustainable, it is necessary to study ethics that can be applied to a Metaverse service platform. In this paper, Metaverse ethics and the rules for applying to the platform are explored. And, in order to judge the ethicality of avatar actions in social Metaverse, the identity, interaction, and relationship of an avatar are investigated. Then, an action-based audit approach to avatar interactions (e.g., dialogues, gestures, facial expressions) is introduced in two cases that an avatar enters a digital world and that an avatar requests the auditing to subjects, e.g., avatars controlled by human users, artificial intelligence (AI) avatars (e.g., as conversational bots), and virtual objects. Pseudocodes for performing the two cases in a system are presented and they are examined based on the description of the avatars' actions.

• The Journal of Korean Association of Computer Education
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• v.11 no.2
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• pp.89-98
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• 2008

Providing appropriate interactions between learner and e- Learning system is an essential factor of a successful e-Learning system. Although many interaction functions are employed in multimedia Web-based Instruction content, learner experience a lack of similar feedbacks from educators in real- time due to the limitation of Human-Computer Interaction techniques. In this paper, an emotion recognition system via learner facial expressions has been developed and applied to a tutoring system. As human educators do, the system observes learners' emotions from facial expressions and provides any or all pertinent feedback. And various feedbacks can bring to motivations and get rid of isolation from e-Learning environments by oneself. The test results showed that this system may provide significant improvement in terms of interesting and educational achievement.

• Journal of Internet Computing and Services
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• v.12 no.6
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• pp.105-116
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• 2011

This paper identifies the determinants of adoption of e-government services. The on-line tax filing and payment system, called Hometax, is a well-known e-government service for taxpayers. Using a theoretical model based on Technology acceptance model, this case study examines the causal relationships among the variables of the adoption behavior for on-line tax filing and payment system. Major research variables include satisfaction, perceived usefulness, perceived ease of use, words of mouth, subjective norms, trust, interaction and facilitating conditions. Results from 279 survey responses indicate that the usefulness and ease of use affect the user satisfaction, which, in turn, with the word of mouth influences intentions to use an e-government service. Subjective norms and trust has been found to affect the usefulness, and interaction as well as facilitating conditions have also some impact on the ease of use. As a conclusion, the academic and practical implications of these findings are discussed.

• Biomolecules & Therapeutics
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• v.30 no.6
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• pp.593-602
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• 2022

The human papillomavirus (HPV)-18 E7 (E7) oncoprotein is a major transforming protein that is thought to be involved in the development of cervical cancer. It is well-known that E7 stimulates tumour development by inactivating pRb. However, this alone cannot explain the various characteristics acquired by HPV infection. Therefore, we examined other molecules that could help explain the acquired cancer properties during E7-induced cancer development. Using the yeast two-hybrid (Y2H) method, we found that the Elk-1 factor, which is crucial for cell proliferation, invasion, cell survival, anti-apoptotic activity, and cancer development, binds to the E7. By determining which part of E7 binds to which domain of Elk-1 using the Y2H method, it was found that CR2 and CR3 of the E7 and parts 1-206, including the ETS-DNA domain of Elk-1, interact with each other. As a result of their interaction, the transcriptional activity of Elk-1 was increased, thereby increasing the expression of target genes EGR-1, c-fos, and E2F. Additionally, the colony forming assay revealed that overexpression of Elk-1 and E7 promotes C33A cell proliferation. We expect that the discovery of a novel E7 function as an Elk-1 activator could help explain whether the E7 has novel oncogenic activities in addition to p53 inactivation. We also expect that it will offer new methods for developing improved strategies for cervical cancer treatment.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.10b
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• pp.273-274
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• 2002

In order to evaluate the flying characteristics of slider, the acoustic emission (AE) as well as friction signals are typically utilized. In this work the frequency spectrum analysis is performed using the AE signal obtained during the head/disk interaction such as load/unload mechanism using ramp, impact situation in the presence of a bump on disk surface and other contact phenomena including particle interaction. It was shown that the influence of impact can be characterized effectively in the AE frequency spectrum. As a result of this work, frequency spectrum analysis will be utilized with better understanding for studying the head/disk interface (HDI) characteristics and monitoring the particle interaction in HDI effectively.

• BMB Reports
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• v.28 no.4
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• pp.363-367
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• 1995

E. coli methionyl-tRNA synthetase is one of the class I tRNA synthetases. The Tryptophane residue at the position 461 located in the C-terminal domain of the enzyme is a key amino acid for the interaction with the anticodon of $tRNA^{Met}$. W461 was replaced with other amino acids to determine the chemical requirement for the interaction with the anticodon of $tRNA^{Met}$. Saturation mutagenesis at the position 461 generated a total of 12 substitution mutants of methionyl-tRNA synthetase. All the mutants showed the same in vivo stability as the wild-type enzyme, suggesting that the amino acid substitutions did not cause severe conformational change of the protein The mutants containing tyrosine, phenylalanine, histidine and cysteine substitutions showed in vivo activity while all the other mutants did not. The comparison of the in vitro aminoacylation activities of these mutants showed that aromatic ring structure, Van der Waals volume and hydrogen bond potential of the amino acid residue at the position 461 are the major determinants for the interaction with the anticodon of $tRNA^{Met}$.

• The Journal of the Acoustical Society of Korea
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• v.25 no.2E
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• pp.85-88
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• 2006

Blade-vortex interaction (BVI) is one of the most important phenomena in rotor flow since it causes undesirable intense vibration and noise. Since three dimensional Euler or Navier-Stokes solutions to BVI require very high computational cost, BVI has been approximated by airfoil-vortex interaction (AVI) in chordwise planes. To describe more realistic situations with AVI, three dimensional vortex informations such as position, core size and strength are embedded artificially to Computational Aeroacoustics (CAA) calculation at each computational time step. To implement this requirement, in this paper, a technique called free vortex embedded method was used. And the solution by this method was compared with the solution by conventional method for interaction between freely convected vortex and airfoil. For the application to three dimensional free vortex embedded CAA, two dimensional free vortex embedded CAA method was validated in advance.

• Structural Engineering and Mechanics
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• v.13 no.3
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• pp.241-260
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• 2002

The effect of shear coupled with axial force variation on the inelastic seismic behaviour of reinforced concrete bridge piers is investigated in this paper. For this purpose, a hysteretic axial-shear interaction model was developed and implemented in a nonlinear finite element analysis program. Thus, flexure-shear-axial interaction is simulated under variable amplitude reversed actions. Comparative studies for shear-dominated reinforced concrete columns indicated that a conventional FE model based on flexure-axial interaction only gave wholly inadequate results and was therefore incapable of predicting the behaviour of such members. Analysis of a reinforced concrete bridge damaged during the Northridge (California 1994) earthquake demonstrated the importance of shear modelling. The contribution of shear deformation to total displacement was considerable, leading to increased ductility demand. Moreover, the effect of shear with axial force variation can significantly affect strength, stiffness and energy dissipation capacity of reinforced concrete members. It is concluded that flexure-shear-axial interaction should be taken into account in assessing the behaviour of reinforced concrete bridge columns, especially in the presence of high vertical ground motion.