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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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The Bacteriophage λ DNA Replication Protein P Inhibits the oriC DNA- and ATP-binding Functions of the DNA Replication Initiator Protein DnaA of Escherichia coli

  • Datta, Indrani (Department of Biochemistry, Bose Institute) ;
  • Sau, Subrata (Department of Biochemistry, Bose Institute) ;
  • Sil, Alok Kumar (Department of Microbiology, University College of Science) ;
  • Mandal, Mitai C. (Department of Biochemistry, Bose Institute)
  • Published : 2005.01.31
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Abstract

Under the condition of expression of $\lambda$ P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the $\lambda$ P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the $\lambda$ P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of $\lambda$ P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.

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References

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