• KSBB Journal
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• 제14권1호
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• pp.31-35
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• 1999

Penicillium janthinellum의 생균체와 변형 균체를 사용한 azo와 reactive계 염료의 생흡착에 대한 연구가 수행되었다. 반응액의 pH가 초기 흡착율과 탈색에 영향을 주어, 최적 pH는 3.0이었으며, 최적 온도는 $40^{\circ}C$이었다. Reactive계 염료인 Apollocion Red 7EB와 Apollfix Red SF-3B와 Apollocion Red HE-3B는 각각 초기 흡착율이 0.06, 0.086, 0.07mg/g.mm이었다. Azo와 reactive 계 염료들을 함유한 혼합염료도Pen janthinellum 균체에 흡착되어 0.084mg/g.min의 초기 흡착율을 보였다. Detergent 첨가시 탈색 백분율과 초기 흡착율은 대조군의 경우보다 낮았다. 또한 이온의 첨가는 염료의 흡착율에 영향을 미치지 않았다 Pen janthinellum의 변형 균체도 염료의 흡착율을 보였고, Apollocion Red 7EB의 흡착에 있어 이온교환수지보다 월등한 흡착능을 보였다.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.544-552
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• 2002

Several microorganisms from rat and human feces and lumen fluid of cows were screened for their ability to decolorize the synthetic dyes. Consequently, a novel dye-degrading strain AB&J was isolated. Taxonomic identification including 165 rDNA sequencing and phylogenetic analysis indicated that the isolate had 99.9% homology in its 165 rDNA base sequence with Clostridium perfringens. After 27 h Incubation with the strain, brilliant blue R, bromophenol blue, crystal violet, malachite green, methyl green, and methyl orange were decolorized by about 69.3%, 97.7%, 96.3%, 97.9%, 75.1%, and 97.2%, respectively. The triphenlmethane dye, bromophenol blue, was decolorized extensively by growing Clostridium perfringens AB&J cells in liquid cultures under anaerobic condition, although their growth was strongly inhibited in the initial stage of incubation. This group of dyes is toxic, depending on the concentration used. The dye was significantly decolorized at a relatively lower concentration of below 50 $\mu g \;ml^{-1}$, however, the growth of the cells was mostly suppressed at a dye concentration of 100 $\mu g \;ml^{-1}$. The decolorization activity in cell-free extracts was much higher in cytoplasm than in periplasm and cytoplasmic membrane. Therefore, the enzyme related uptake of bromophenol blue seemed to be localized in cytoplasm. The optimal pH and temperature of bromophenol blue uptake fur decolorization activities were 7.0 and 4$0^{\circ}C$, respectively.

• Environmental Engineering Research
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• 제25권4호
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• pp.561-570
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• 2020

In the present study, dye decolorizing bacteria were isolated from water and soil samples, collected from textile industries in Jodhpur province, India. Two bacterial species namely, Bacillus pumilis and Paenibacillus thiaminolyticus were screened and identified based on biochemical characterization. The degradation efficiency of these two microorganisms was compared through optimization of pH, incubation time, initial dye concentration and inoculum size. B. pumilis and P. thiominolyticus were able to degrade 61% and 67% Red HE3B, 81% and 75% Orange F2R, 49.7% and 44.2% Yellow ME4GL and 61.6% and 59.5% Blue RC CT dyes of 800mg/l concentration respectively. The optimum pH and time were found to be 8 within 24 hours. The FT-IR analysis confirmed that microorganisms were able to degrade toxic azo dyes into a non-toxic product as proved through structural modifications to analyze chemical functions in materials by detecting the vibrations that characterize chemical bonds. It is based on the absorption of infrared radiation by the microbial product. Therefore, Bacillus pumilis and Paenibacillus thiaminolyticus are a promising tool for decolorization of dyes due to its potential to effectively decolorize higher azo dye concentrations (10-800 mg/L) and can be exploited for bioremediation.

• 한국환경농학회지
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• 제25권3호
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• pp.211-216
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• 2006

Coriolus hirsutus LD-1 균주의 리그닌분해효소 활성과 몇몇 염료의 탈색능을 조사하였다. 백색부후균인 LD-1 균주는 laccase(16,388.9 U/L)와 manganese- dependent peroxidase (19.81 U/L)는 생산하였으나 lignin peroxidase를 생산하지 않았다. 균주를 염료와 함께 8일간 배양했을때 염료 RBBR과 염료 BB의 탈색율은 각각 70.2%와 98%로 나타났다. Manganese-dependent peroxidase는 8일간 배양 중 효소 활성은 매우 낮은 반면 laccase는 지속적으로 생산되어 대단히 높은 활성을 나타내었다. 백색부후균인 Coriolus hirsutus LD-1에 의한 염료의 탈색은 주로 laccase에 의한 것으로 사료되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.403-408
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• 2003

A newly isolated Rhodopseudomonas palustris P4 could decolorize various synthetic dyes containing different chromogenic groups such as azo linkage (Crocein Orange G, New Coccine, Chromotrope FB, Congo Red, Remazol Black B), anthraquinone Reactive blue 2, or indigo Indigo Carmine. Among them, the degradation rate of Black B was studied in detial. Degradation of Black B followed the Arrhenius equation in 25 - $40^{\circ}C$ with an activation energy of 7.79 kcal/mol. Optimum pH was 8. Glucose in the range of 5 - 50g/l did not affect the Black B decolorization. When Black B increased from 25 mg/l to 2000 mg/l, decolorization activity increased almost linearly but the extent of decolorization was constant at about 86% irrespective of dye concentration. Analyses by HPLC revealed that the Black B molecules were partially degraded and some chromogenic intermediates were produced. These results indicate that Rps. palustris P4 has an outstanding capability to degrade various dyes.

• Journal of the Korean Wood Science and Technology
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• 제32권3호
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• pp.79-87
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• 2004

The objectives of this study were to select superior fungus for lignin degradation and to decolor dyes by selected fungus. Ligninolytic fungi were screened and isolated from decayed woods. Ten ligninolytic fungi were selected by ligninolytic enzyme activity on the PDA media containing rhemazol brilliant blue R, guaiacol and gallic acid. Their lignin degradation abilities were tested on the extractive-free wood powder of Quercus acutissima and Pinus densiflora. As a result, 8J-28 was selected as superior fungus for lignin degradation. Also, decolorization abilities of dyes were examined by shaking and static culture. And congo red, crystal violet, poly R-478, methylene blue used to investigate decolorization abilities of dyes. As a result, 8J-28 showed over 90% in decolorization of congo red, crystal violet, poly R-478.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.500-508
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• 1999

Several white-rot fungi collected from the mountains of Korea were evaluated for their ability to decolorize azo, polymeric, and reactive dyes. Strains CJ-105, CJ-212 and CJ-315, identified as Trametes sp., Pleurotus sp. and Fomes sp., respectively, showed higher potential for decolorization of those dyes in either solid or liquid media. For Trametes sp. CJ-105, 100ppm of Remazol Brilliant blue R and 500ppm of Acid Red 264 were completely decolorized after 2 days under liquid culture. The dominating ligninolytic enzyme existing in the culture broth was laccase (E.C. 1.10.3.2). Also, Pleurotus sp. CJ-212 and Fomes sp. CJ-315 showed similar patterns in decolorization of Remazol Brilliant Blue R and Acid Red 264. The extent of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic of decolorization of the dyes in liquid culture was found to be proportional to the activities of the ligninolytic enzymes produced by each strain. In addition to that Trametes sp. CJ-105 was highly effective in degradation of polycyclic aromatic hydrocarbons and pentachlorophenol by the activity of the ligninolytic enzymes produced. In this study, we found that white-rot fungi, Trametes sp. CJ-105(KFCC 10941), Pleurotus sp. CJ-212(KFCC 10943) and Fomes sp. CJ-315(KFCC 10942), were effective in decolorizing a wide range of structurally different synthetic dyes, as well as some chemical compounds which are known to be hardly degradable.

• 한국균학회지
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• 제42권3호
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• pp.213-218
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• 2014

높은 laccase 활성을 보유한 큰느타리 수확 후 배지추출물(SMSE)은 bromophenol blue과 remazol brillient blue R염료에서 각각 93.7%와 88.7%의 높은 탈색효과를 나타내었다. 그 외의 Congo red는 72.13%을 보인 반면에 다른 염료는 60% 내의 탈색효과를 나타내었다. 특히 직물염색에 사용되는 Rit (red)와 Rit (blue)는 51.6%와 30.4%의 비교적 낮은 탈색율을 보였으며 Methylen blue 염료는 탈색률이 검출되지 않았다. 염료는 $20{\sim}30^{\circ}C$의 온도에서 가장 높은 탈색률을 보였고, $50^{\circ}C$까지 높은 염료 탈색효과를 보였다. 시간에 따른 염료 탈색효과 실험에서 bromophenol blue와 remazol brillient blue R은 SMSE 처리 1시간 후에 반응이 나타나기 시작하여 12시간 후에는 80% 이상의 높은 탈색율을 보였으나 다른 염료들은 탈색 정도가 서서히 증가되어 24시간 후 최고 60%의 탈색율을 보였다. 큰느타리 SMSE는 섬유공장으로부터 수집한 산업 폐 염료의 탈색효과를 나타내어 환경친화적인 염료탈색의 산업적 이용성을 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.843-849
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• 2013

A dye-decolorizing bacterium was isolated from a soil sample and identified as Bacillus thuringiensis using 16S rRNA sequencing. The bacterium was able to decolorize three different textile dyes, namely, Reactive blue 13, Reactive red 58, and Reactive yellow 42, and a real dyehouse effluent up to 80-95% within 6 h. Some non-textile industrially important dyes were also decolorized to different extents. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds to yield sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene. Sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) was further oxidized by the ortho-cleavage pathway to yield 2-(1-amino-2-diazenyl-2-formylvinyl) benzoic acid. There was induction of the activities of laccase and azoreductase during the decolorization of Congo red, which suggests their probable role in the biodegradation. B. thuringiensis was found to be versatile and could be used for industrial effluent biodegradation.

• 한국환경과학회지
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• 제13권10호
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• pp.921-928
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• 2004

The photocatalytic decolorization of Rhodamine B (RhB) was studied using immobilized $TiO_2$ and fluidized bed reactor. Immobilized $TiO_2$(length: 1$\~$2 mm, width: 1$\~$3 mm, thickness: 0.5$\~$2 mm) onto silicone sealant was employed as the photocatalyst and a 30 W germicidal lamp was used as the light source and the reactor volume was 4.8 L. The effects of parameters such as the amounts of photocatalyst, initial concentration, initial pH, superficial velocity, $H_2O_2$ and anion additives. ($NO_3^{-},\;SO_4^{2-},\;Cl^{-},\;CO_3^{2-}$) The results showed that the optimum dosage of the immobilized $TiO_2$ were 87.0 g/L. Initial removal rate of RhB of the immobilized $TiO_2$ was 1.5 times higher than that of the powder $TiO_2$ because of the adsorption onto the surface of immobilized $TiO_2$ In the conditions of acidic pH, initial reaction rate was increased slowly and reaction time was shorted. The effect of anion type on the reaction rate was not much.