Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Production of Lignin Degrading Enzymes and Decolorization of Dye Compounds by White-rotting Fungi Coriolus hirsutus LD-1

백색부후균 Coriolus hirsutus LD-1의 리그닌분해효소 활성과 염료탈색에 관한 연구

  • Nam, Eun-Sook (Department of Food and Nutrition, Kyungwon University) ;
  • Ha, Sang-Woo (Department of Food and Nutrition, Kyungwon University) ;
  • Park, Shin-In (Woogene Central Research Lab.)
  • Published : 2006.09.30
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Abstract

The present research was undertaken to investigate the activities of ligninolytic enzymes and dye-decolorization capabilities of white-rotting fungi Coriolus hirsutus LD-1. The isolated white-rotting fungi (Coriolus hirsutus LD-1) produced laccase (16,388.9 U/L) and manganese-dependent peroxidase (19.81 U/L) but it did not produce lignin peroxidase. When the isolated fungi was incubated with the treatment of dyes for 8 days, the rates of decolorization of remazol brilliant blue R and bromophenol blue were 70.2% and 98%, respectively. The activity for manganese-dependent peroxidase was low, whereas that for laccase was very high. Moreover, the laccase was more effective to decolor when compared to manganese-dependent peroxidase. The results suggested that laccase of Coriolus hirsutus LD-1 might be playing an important role in the decolorization of the dyes.

Coriolus hirsutus LD-1 균주의 리그닌분해효소 활성과 몇몇 염료의 탈색능을 조사하였다. 백색부후균인 LD-1 균주는 laccase(16,388.9 U/L)와 manganese- dependent peroxidase (19.81 U/L)는 생산하였으나 lignin peroxidase를 생산하지 않았다. 균주를 염료와 함께 8일간 배양했을때 염료 RBBR과 염료 BB의 탈색율은 각각 70.2%와 98%로 나타났다. Manganese-dependent peroxidase는 8일간 배양 중 효소 활성은 매우 낮은 반면 laccase는 지속적으로 생산되어 대단히 높은 활성을 나타내었다. 백색부후균인 Coriolus hirsutus LD-1에 의한 염료의 탈색은 주로 laccase에 의한 것으로 사료되었다.

Keywords

References

  1. Belsare, D. K. and Prasad, D. Y. (1988) Decolorization of effluent from the bagasse-base pulp mills by white-rot fungus, Schizophyllum commune. Appl. Microbiol. Biotechnol. 28, 301-309
  2. Cripps, C., Bumpus, J. A. and Aust S. D. (1990) Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 56, 1114-1118
  3. Fukuzumi, T. (1982) Microbial decolorization and defoaming of pulping waste liquors. In: Kirk, T. K., Higuchi, T. and Chang, H. M. (eds) Lignin degradation : microbiology, chemistry and potential applications, Vol. 1, RC Press, Boca Raton, Fla., p.215
  4. Blanchette, R. A. (1984) Screening wood decayed by white rot fungi for preferntial lignin degradation. Appl. Environ. Microbiol. 46, 647-653
  5. Heiss, G. S., Gowan, B. and Dabbs, E. R. (1992) Cloning of DNA from Rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. FEMS Microbiol. Lett. 99, 221-226 https://doi.org/10.1111/j.1574-6968.1992.tb05571.x
  6. Han, S. and Yang, H. (1988) Decolorization of acid red B by immobilized cells of Bacillus cereus No.45. Huanjing Kexue Xuebao. 8, 93-98
  7. Idaka, E., Ogawa, T., Horitsu, H. and Tomoyeda, M. (1978) Degradation of azo compounds by Aeromonas hydrophilia var. 24B. J. Soc. Dyes Colour. 94, 91-94
  8. Zimmermann, T., Kulla, H. G. and Leisinger, T. (1982) Properties of purifies orange II azo reductase, the enzyme initating azo dye degradation by Pseudomonas KF46. J. Biochem. 129, 197-203
  9. Glenn, J. K. and Gold, M. H. (1983) Decolorization of several polymeric dyes by the lignin-degradation basidiomycete Phanerochaete chrysosporium. Appl. Environ. Microbiol. 45, 1741-1747
  10. Pauli, O., Kirsi, A., Veli-Matti, L., Tuomo, G., Timo, R. and Ilari, S. (1993) Decolorization of azo, triphenyl methane, heterocyclic and polymeric dyes by lignin peroxidase isoenzymes from Phanerochaete chrysosporium. Appl. Environ. Microbiol. 59, 4010 -4016
  11. Colleen, C., John, A. B. and Steven, D. A. (1990) Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 56, 1114-1118
  12. Griselda, G. D. and Eduardo, A. T. (1990) Screening of white-rot fungi for efficient decolorization of bleach pulp effluents. Biotechnol. Lett. 12, 869-872 https://doi.org/10.1007/BF01022613
  13. Cao, W., Mahadevan, B., Crawford, D. L., Goszczynski, S., Crawford, D. L. and Crawford, R. L. (1992) Characterium of an extracellular azo dyeoxidizing peroxidase from Flavobacterium sp. ATCC 39723. Enzyme Micorb. Technol. 15, 810-817
  14. Zhou, W. and Zimmerman, W. (1993) Decolorization of industrial effluents contacting reactive dyes by Actinomecetes. FEMS Microbiol. Lett. 107, 157-162 https://doi.org/10.1111/j.1574-6968.1993.tb06023.x
  15. Fredrick, S. A., Michael, G. P. and Lubomir, J. (1990) Decolorization of kraft bleachery effluent chroco phores by Coriolus (Trametes) versicolor. Enzyme Microb. Technol. 12, 846-853 https://doi.org/10.1016/0141-0229(90)90021-H
  16. Kotterman, M. J. J., Heesseis, E., Jong, E. D. and Field, J. A. (1994) Thephysiology of anthraene biodegradation by the white-rot fungus Bjerkandera sp. strain BOS55. Appl. Microbiol. Biotechnol. 42, 179-186 https://doi.org/10.1007/BF00170243
  17. Arora, D. S. and Sandu, D. K. (1986) Degradation of lignocellulotic residures by polyporus versicolor and the effect of moisture contents and phenolic compounds. Acta Biotechnol. 6, 293-297 https://doi.org/10.1002/abio.370060323
  18. Ritter, D., Jaklin-Farcher, S., Messner, K. and Stachellberger. H. (1990) Polymerization and depolymerization of lignosulfonates by Phanerochaete chrysosporium immobilized on foam. J. Biotechnol. 13, 229-241 https://doi.org/10.1016/0168-1656(90)90108-N
  19. Eggert, C., Temp, U. and Eriksson, K. L. (1996) The lignolytic system of the white rot fungus Phanerochaete chrysosporium : Purification and characterization of the laccase. Appl. Environ. Microbiol. 62, 1151-1158
  20. de Jong, E., Field, J. A. and de Bont, J. A. M. (1992) Evidence for a new extracellular peroxidase manganase-inhibited peroxidase from the white rot fungus Bjerkandera sp. BOS55. FEBS Lett. 299, 107-110 https://doi.org/10.1016/0014-5793(92)80111-S
  21. Kirk, T. K. and Connors, W. J. (1987) Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Arch. Microbiol. 117(3), 277-285 https://doi.org/10.1007/BF00738547
  22. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. L. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275
  23. Jung H. C., Park, S. K., Kim, B. S. and Park, C. Y. (1995) Screening and evaluation of wood-rotting fungi for linin degradation and ligninolytic enzyme production (1). Mokchae Konhak 23(4), 108-116
  24. Nishida, T., Kashino, Y., Mimura, A. and Takahara, Y. (1998) Lignin biodegradation by wood-rotting fungi. I. Screening of lignin-degrading fungi. 161, 264-27
  25. 정형재, 박서기, 김병수, 박종열. (1996) 리그닌분해와 리그닌분해 효소생산을 위한 목재 부후균의 선발과 평가 (II). 목재공학 24(4), 74-81
  26. Kim, S. J., Ishikawa, K., Hirai, M. and Shoda, M. (1995) Characteristics of a newly isolated fungus, Geotrichum candidum Dec I, which decolorizes various dyes. J. Ferment. Bioeng. 79, 601-607 https://doi.org/10.1016/0922-338X(95)94755-G
  27. Manzanares, P., Fajardo, S. and Martin, C. (1995) Production of ligninolytic activities when treating paper pulp effluents by Trametes versicolor. J. Biotechnol. 43, 125-132 https://doi.org/10.1016/0168-1656(95)00125-8
  28. Hatakka, A. (1994) Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol. Rev. 13, 125- 135 https://doi.org/10.1111/j.1574-6976.1994.tb00039.x

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