• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4469-4475
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• 2015

Transient receptor potential melastain 7 (TRPM7) is a bifunctional protein with dual structure of both ion channel and protein kinase, participating in a wide variety of diseases including cancer. Recent researches have reported the mechanism of TRPM7 in human cancers. However, the correlation between TRPM7 and prostate cancer (PCa) has not been well studied. The objective of this study was to investigate the potential the role of TRPM7 in the apoptosis of PC-3 cells, which is the key cell of advanced metastatic PCa. In this study, we demonstrated the influence and potential function of TRPM7 on the PC-3 cells apoptosis induced by TNF-related apoptosis inducing-ligand (TRAIL). The study also found a novel up-regulated expression of TRPM7 in PC-3 cells after treating with TRAIL. Suppression of TRPM7 by TRPM7 non-specific inhibitors ($Gd^{3+}$ or 2-aminoethoxy diphenylborate (2-APB) ) not only markedly eliminated TRPM7 expression level, but also increased the apoptosis of TRAIL-treated PC-3 cells, which may be regulated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway accompany with up-regulated expression of cleaved Caspase-3, (TRAIL-receptor 1, death receptors 4) DR4, and (TRAIL-receptor 2, death receptors 5) DR5. Taken together, our findings strongly suggested that TRPM7 was involved in the apoptosis of PC-3 cells induced by TRAIL, indicating that TRPM7 may be applied as a therapeutic target for PCa.

• The KIPS Transactions:PartB
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• v.19B no.2
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• pp.99-106
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• 2012

The purpose of this study was to determine the evaluation parameters' osteoporosis predictability in accordance with measuring regions by analyzing the correlations between bone mineral density and trabecular patterns derived from different measuring regions. Experimental subjects were a total of 40 female patients after menopause aged over 40 years, and were classified into 20 control and 20 osteoporotic groups according to the T-score. Bone mineral density was measured on femoral neck, trochanter and ward's triangle by DEXA(Dual Energy X-ray Absorptiometry). We designated ROI(Region of Interest) with $50{\times}50$ pixel size on each measuring regions, and extracted trabecular patterns by using existing image processing method. We also selected a total of eight evaluation parameters that are categorized into structural(mean gray level, area, perimeter, thickness and terminal distance), skeletonized parameters(number, length) and fractal dimension. As a result, it was observed that area, perimeter, thickness, terminal distance, number, length and fractal dimension reflected the bone mineral density with high statistical validity(p<0.003). We also confirmed that the evaluation parameters could predict the osteoporosis more efficiently.

• Clinical and Experimental Pediatrics
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• v.46 no.10
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• pp.958-965
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• 2003

Purpose : Recently the number of juvenile delinquents have been increasing in Korea and their misdeeds were getting worse. This study was conducted to find out the basic cause of juvenile delinquency and a counterplan of overcoming this distress. Methods : The authors reviewed the statistics of the Supreme Public Prosecutors Office to know the juvenile delinquents and Korean national statistics to know the changes of family environments. The author conducted a study on the family environments between student group and juvenile delinquent group in jail, and also investigated the value judgement of middle and high school students to know the difference between the recent adolescents and the youth before 1945. Results : Nuclear families, dual-income families and divorce rates have remarkably been increased recently. All of which are considered to be the basic cause of getting worse in home teaching. It was found that the delinquent group have more defective families, poorer economic status, poorer parent's educational level than the student group, and also found that the value judgement of recent youth were quite different from that of youth before 1945. Conclusion : Industrialization have changed our home-environment rapidly which made home-teaching poorer, and the children were being insecure and melancholy, which consequently made them more abnormal youth and juvenile delinquents in Korea.

• Korean Journal of Community Nutrition
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• v.21 no.4
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• pp.378-385
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• 2016

Objectives: This study was conducted to investigate the association between sarcopenia and sarcopenic obesity and cardiovascular disease risk in Korean postmenopausal women. Methods: We analyzed data of 2,019 postmenopausal women aged 50-64 years who participated in the Korea National Health and Nutrition Examination Survey in 2008-2011 and were free of cardiovascular disease history. Blood pressure, height, and weight were measured. We analyzed the serum concentrations of glucose, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglyceride levels. Waist circumference was used to measure obesity. Appendicular skeletal muscle mass was measured by dual-energy X-ray absorptiometry. Sarcopenia was defined as the appendicular skeletal muscle mass/body weight<1 standard deviation below the gender-specific means for healthy young adults. The estimated 10-year risk of cardiovascular disease risk was calculated by Pooled Cohort Equation. Subjects were classified as non-sarcopenia, sarcopenia, or sarcopenic obesity based on status of waist circumference and appendicular skeletal muscle mass. Results: The prevalence of sarcopenia and sarcopenic obesity was 16.3% (n=317) and 18.3% (n=369), respectively. The 10-year risk of cardiovascular disease risk in the sarcopenic obesity group was higher ($3.82{\pm}0.22%$) than the normal group ($2.73{\pm}0.09%$) and sarcopenia group ($3.17{\pm}0.22%$) (p < 0.000). The odd ratios (ORs) for the ${\geq}7.5%$ 10-year risk of cardiovascular disease risk were significantly higher in the sarcopenic obesity group (OR 3.609, 95% CI: 2.030-6.417) compared to the sarcopenia group (OR 2.799, 95% CI: 1.463-5.352) (p for trend < 0.000) after adjusting for independent variables (i.e., exercise, period of menopausal, alcohol use disorders identification test (AUDIT) score, income, education level, calorie intake, %fat intake and hormonal replacement therapy). Conclusions: Sarcopenia and sarcopenic obesity appear to be associated with higher risk factors predicting the 10-year risks of cardiovascular disease risk in postmenopausal women. These findings imply that maintaining normal weight and muscle mass may be important for cardiovascular disease risk prevention in postmenopausal women.

• Journal of the Korean Home Economics Association
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• v.43 no.12 s.214
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• pp.61-78
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• 2005

This study was peformed to assess the relationships among bone mineral density(BMD), nutrient intake and lifestyle factors in postmenopausal women. A trained nutritionist examined the interviewed usual dietary intake and physical activity of 85 subjects in Chungnam with a questionnaire interview, and BMDs of the lumbar spines($L_2-L_4$), femoral necks(FN), ward's triangles(WT) and trochanters(TR) were measured by dual energy X-ray absorptiometry (DEXA). The BMDs(T-score) of $L_2-L_4$, FN, and WT were $0.996g/cm^2(-1.601),\;0.697g/cm^2(-1.697)$, and $0.793g/cm^2(-1.512)$, respectively, which were assessed as osteopenia by T-score,. and TR was normal at $0.718g/cm^2(-0.675)$. Subjects included 85 in Chungnam were divided into three groups according to the BMD measurement of the $L_2-L_4$ and FN assessed by T-score. The percentages of the osteoporosis, osteopenia, and normal groups were $32.9\%,\;42.4\%$ and $24.7\%$ respectively. The average age was significantly the highest of the osteoporosis group than in the other two osteopenia or normal groups(P<0.001). Among lifestyle factors, the BMD of lifestyles was significantly related with exercise but it was not significant with medication., salt. intake, bone fracture, coffee consumption, drinking and smoking. The nutrient intake of the subjects was most nutrient intake adequate to the Korean RDA level for most nutrients excepting energy intakes and calcium. The BMD of lumbar spines $L_2-L_4$ showed significant positive association with the intake of most nutrients except carbohydrate, especially in the normal group. The Higher BMD levels were found for those with high intake of protein and vitamin B1. The BMDs of $L_2-L_4$, and FN were positively correlated with showed energy expenditure, exercise, miscellaneous activity, per weekend and week(P<0.05) in normal group but that was showed negatively correlated in the osteopenia group. Therefore, this study confirmed that one of the most effective ways to minimize bone less in postmenopausal women is to maintain an adequate intake of calcium and other nutrients and regular physical activity.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1103-1109
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• 2018

Objective: This study aimed to screen and identify the target genes of miR-375 in pig Sertoli (ST) cells and to elucidate the effect of miR-375 on the proliferation of ST cells. Methods: In this study, bioinformatics software was used to predict and verify miR-375 target genes. Quantitative polymerase chain reaction (PCR) was used to detect the relationship between miR-375 and its target genes in ST cells. Enzyme-linked immunosorbent assay (ELISA) of rearranged L-myc fusion (RLF) and hypoxia-induced gene domain protein 1A (HIGD1A) was performed on porcine ST cells, which were transfected with a miR-375 mimics and inhibitor to verify the results. Dual luciferase reporter gene assays were performed to assess the interactions among miR-375, RLF, and HIGD1A. The effect of miR-375 on the proliferation of ST cells was analyzed by CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS). Results: Five possible target genes of miR-375, including RLF, HIGD1A, colorectal cancer associated 2, POU class 3 homeobox 1, and WW domain binding protein 1 like, were found. The results of quantitative PCR suggested that mRNA expression of RLF and HIGD1A had a negative correlation with miR-375, indicating that RLF and HIGD1A are likely the target genes of miR-375. The ELISA results revealed that RLF and HIGD1A were negatively correlated with the miR-375 protein level. The luminescence results for the miR-375 group cotransfected with wild-type RLF and HIGD1A vector were significantly lower than those of the miR-375 group co-transfected with the blank vector or mutant RLF and HIGD1A vectors. The present findings suggest that RLF and HIGD1A are target genes of miR-375 and that miR-375 inhibits ST cell proliferation according to MTS analysis. Conclusion: It was speculated that miR-375 affects cell proliferation through its target genes, which play an important role in the development of testicular tissue.

• Korea journal of population studies
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• v.21 no.2
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• pp.37-57
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• 1998

In recent, Korea introduced the local self-government system. And the local government tried to establish and implement plans for women. Those plans were established on the basis of not the particular environments of regional societies but similar policies of central government. Despite of geographical migrations, we can find differences of population structure and economic status among regions. As the result, most of regional policies for women were ineffective. This paper examines population structure and economic status of women by regions and recommends the long and medium direction for local policies for women. For analysis, it utilizes the 2% sample data-set of National Statistical Office's '1995 Population and Housing Census' in Korea. The findings of this paper are summarized as following; Firstly, the dependency rate of Chungchong-do, Cholla-do, Kyongsangnam-do will reach about 45% in 2000. Therefore, as soon as possible, those local government should establish the labor policies to enhance the economically active participation rate of the elderly persons and welfare policy for them. Secondly, the level of women's economic participation. Thus, local governments should set up labor market policies for women according to regional situations. For examples, in Kyonggi-do, Seoul, Daejeon, Daeku which unemployment rate of the highly educated women are relatively high, those local governments should seek the measures to tackle unemployment problem of them. Thirdly, due to the differences regional economic structure, women's employment structures differed also by region. Hence, local governments should formulate suitable plans to consider the working status of regional women's workers.

• The Journal of the Korea Contents Association
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• v.12 no.6
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• pp.353-360
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• 2012

The purpose of this study was to determine whether there are relationships between radiologic parameters for osteoporosis by the proximal femur radiograhps and bone mineral density, as assessed by Dual Energy X-ray Absorptiometry(DEXA). Proximal femur anterior-posterior projection were taken from 112 individuals who undergoing DEXA. Radiographic parameters including canal-to-calcar ratio(CCR) and cortical thickness indices(CTI) were measured and compared with bone mineral density($g/cm^2$), T-score. The intramedullary femoral canal width(FW) and calcar width(CW) at osteoporosis group was significantly larger than that of control group. The CCR were correlated negatively with bone mineral density($g/cm^2$)(r=-0.340, p<0.01), and the CTI were correlated positively(r=0.624). The diagnostic accuracy of CCR for osteoporosis was 63.4%, and kappa value was 0.271. And the accuracy of CTI was 67.0%, and kappa value was the level of fair agreement(${\kappa}$=0.258). The proximal femur with large CCR and small CTI had lower T-scores. When evaluating proximal femur anterior-posterior radiographs, those patients with the thinnest femur diaphyseal cortices should be referred for further osteoporosis investigation.

• Korean Journal of Social Welfare
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• v.52
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• pp.201-221
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• 2003

This paper aims to examine whether there are significant differences in various aspects of a household's arrangements by type of employment of married woman; 1) the extent of the division of labor, 2) the authority of decision making, and 3) financial and expenditure responsibilities. It also investigates the determinants of gender equality in the family. Based on data collected in the fall of 2002 from a representative sample of the Korean population, this study finds that nonstandard employment of married woman including temporary work and daily basis work does not contribute to gender equality within the household, although most of nonstandard employees are full-time workers. However, standard employment of married woman contribute to gender equality in the family. The results of this study show that husbands whose wives are standard-employed are more likely to take part in housework chores that are female-dominated, and standardly employed wives are more likely than non-standardly employed or housewives to take part in the household's financial and expenditure responsibilities. Standardly employed wives also have more power in decision making process within households. On the contrary, non-standardly employed wives gain no advantage over housewives within their families, due to lack of bargaining resources that enable them to affect the household's arrangements. Thus, they have confronted additional burdens, which stem from carrying the dual role of doing house work as well as paid work. Such increasing work-family conflict may bring about disruption of family. Therefore, this study maintains that it is high time that government-level efforts should be made in order to improve the status of irregularly employed wives in the workplace.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.214-226
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• 1992

In this paper, the characteristics of fatigue crack growth in the spot welded joint of the same kinds of specimens($HS{\times}HS,\;GA{\times}GAB$) and different kinds of specimens($HS{\times}GA,\;HS{\times}GAB$) which consist of dual phase high strength steel(HS) and monogalvanized steel(GA) were examined with static tension tests and axial tension fatigue tests. Some of the important results are as follows : 1. The divergence of tensile strengths among the same and different kinds of spot welds under the same conditions is comparatively low regardless of the difference of stiffness. 2. At the low load bevel and long life legion, the fatigue crack is initiated near the nugget. However, in the high load level and short life region, it occurs a tittle far from the nugget. 3. It has shown a linear relation between maximum stress Intensity factor, Kmax and fatigue life, $N_f$ among each of the spot welds and has gathered in a narrow band on the log-log graph paper. $Kmax=H{\cdot}{N_f}^{P}$ where H and P are a material constant.