• Journal of Ginseng Research
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• v.40 no.4
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• pp.315-324
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• 2016

Background: Biocontrol agents are regarded as promising and environmental friendly approaches as agrochemicals for phytodiseases that cause serious environmental and health problems. Trichoderma species have been widely used in suppression of soil-borne pathogens. In this study, an endophytic fungus, Trichoderma gamsii YIM PH30019, from healthy Panax notoginseng root was investigated for its biocontrol potential. Methods: In vitro detached healthy roots, and pot and field experiments were used to investigate the pathogenicity and biocontrol efficacy of T. gamsii YIM PH30019 to the host plant. The antagonistic mechanisms against test phytopathogens were analyzed using dual culture, scanning electron microscopy, and volatile organic compounds (VOCs). Tolerance to chemical fertilizers was also tested in a series of concentrations. Results: The results indicated that T. gamsii YIM PH30019 was nonpathogenic to the host, presented appreciable biocontrol efficacy, and could tolerate chemical fertilizer concentrations of up to 20%. T. gamsii YIM PH30019 displayed antagonistic activities against the pathogenic fungi of P. notoginseng via production of VOCs. On the basis of gas chromatography-mass spectrometry, VOCs were identified as dimethyl disulfide, dibenzofuran, methanethiol, ketones, etc., which are effective ingredients for antagonistic activity. T. gamsii YIM PH30019 was able to improve the seedlings' emergence and protect P. notoginseng plants from soil-borne disease in the continuous cropping field tests. Conclusion: The results suggest that the endophytic fungus T. gamsii YIM PH30019 may have a good potential as a biological control agent against notoginseng phytodiseases and can provide a clue to further illuminate the interactions between Trichoderma and phytopathogens.

• Journal of the Korea Society of Computer and Information
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• v.17 no.9
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• pp.165-169
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• 2012

To analogize the result of the test through explaining the correlation of bone mineral density (BMD) test value between proximal femur and lumbar spine at age. It is based on 62 cases who visited the out-patient department. They were classified into three groups by age, group I (30~40 yr) and II (40~60 yr) and III (60~80 yr). Then we evaluated the average and the degree of correlation between the three groups and analyzed the correlation of the three sites according to the BMD result and T & Z-score through the t-test analysis. The results are listed below, first, if rise in age T-score is lower and over 60age occurred osteopenia, second, compared three groups, the correlation of BMD results was some of the highest between Lumbar spine and Proximal femur of T-score & Z-score. the correlation of BMD results is very high. It is regarded that there will be a clinical availability which can analogize the result of a part by using the result of the other part.

• The Korean Journal of Physiology and Pharmacology
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• v.14 no.4
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• pp.241-248
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• 2010

The present sutdy aimed to determine whether olmesartan, an angiotensin II (Ang II) type 1 ($AT_1$) receptor blocker, can influence the CA release from the isolated perfused model of the rat adrenal medulla. Olmesartan ($5{\sim}50{\mu}M$) perfused into an adrenal vein for 90 min produced dose- and time-dependent inhibition of the CA secretory responses evoked by ACh (5.32 mM), high $K^+$ (56 mM, a direct membrane-depolarizer), DMPP (100 ${\mu}M$) and McN-A-343 (100 ${\mu}M$). Olmesartan did not affect basal CA secretion. Also, in adrenal glands loaded with olmesartan (15 ${\mu}M$), the CA secretory responses evoked by Bay-K-8644 (10 ${\mu}M$, an activator of voltage-dependent L-type $Ca^{2+}$ channels), cyclopiazonic acid (10 ${\mu}M$, an inhibitor of cytoplasmic $Ca^{2+}$-ATPase), veratridine (100 ${\mu}M$, an activator of voltage-dependent $Na^+$ channels), and Ang II (100 nM) were markedly inhibited. However, at high concentrations ($150{\sim}300{\mu}M$), olmesartan rather enhanced the ACh-evoked CA secretion. Taken together, these results show that olmesartan at low concentrations inhibits the CA secretion evoked by cholinergic stimulation (both nicotininc and muscarinic receptors) as well as by direct membrane depolarization from the rat adrenal medulla, but at high concentrations it rather potentiates the ACh-evoked CA secretion. It seems that olmesartan has a dual action, acting as both agonist and antagonist at nicotinic receptors of the isolated perfused rat adrenal medulla, which might be dependent on the concentration. It is also thought that this inhibitory effect of olmesartan may be mediated by blocking the influx of both $Na^+$ and $Ca^{2+}$ into the rat adrenomedullary chromaffin cells as well as by inhibiting the $Ca^{2+}$ release from the cytoplasmic calcium store, which is thought to be relevant to the $AT_1$ receptor blockade, in addition to its enhancement on the CA secreton.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1881-1890
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• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• Journal of Community Nutrition
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• v.1 no.2
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• pp.98-107
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• 1999

The purpose of this study was to investigate Bone Mineral Density(BMD) and affecting factors on BMD of college women in Seoul. The subjects were 47 healthy college women aged 18-25 years. Antrophometric and body fat measurements were performed by Bioelectrical Impedance Fatness Analyzer(Tanita TVF 202). Blood pressure and pulse frequency were measured. Dietary intakes and general living habits were examined through questionnaires and nutrient intakes were analyzed by Computer Aided Nutritional Analysis(CAN) program for professional. Serum total cholesterol, TG(triglyceride), HDL-cholesterol, total protein, albumin, GOT, calcium were measured by Spotchem(SP-4410). Serum osteocalcin and alkaline phosphatase(ALP) were measured to monitor bone formation. BMD of lumbar spine(L2-L4), right hip(neck, ward's triangle, trochanter) and right forearm were measured by Dual Energy X-ray Absorptiometry(DEXA). Muscle strength was measured by examining leg flexion strength(right and left), leg extension power(right and left), handgrip power(right and left) and back strength. All data were statistically analyzed by the SAS PC package program. BMD of college women was normal(by WHO, 1994). Their muscle strength was bad(by national fitness guidebook, 1995). Only a mall number of them exercised (32.6% of subjects). There was no significant difference among BMD, muscle strength and % body fat(p<0.05). There were significant differences between BMD and total cholesterol as well as TG and VLDL-cholesterol(p<0.05). Total cholesterol was associated with decreasing BMD of the right forearm(p<0.05). TG and VLDL-cholesterol are associated with increasing BMD of right hip ward's triangle(p<0.05). There were significant differences among BMD, ALP and serum total protein. ALP was associated with decreasing BMD of the right forearm(p<0.05). There were significant differences between BMD and Ca as well as between Na and K intakes (p<0.05). Intakes of Na and K were associated with decreasing BMD of the right forearm (UD)(p<0.05). There were significant differences between in BMD and pulse frequently and serum albumin (p<0.05). Serum albumin is associated with increasing BMD of L3-L4(p<0.05), right hip neck(p<0.05). %Body fat, TG, VLDL-C, Ca intake, pulse frequency and serum albumin were associated with increasing BMD(p<0.05). Intakes of Na and K, ALP, total cholesterol, total cholesterol, total protein and height are associated with decreasing BMD(p<0.05). Overall results indicate that Ca intake but to be moderate in protein and Na intakes in order to increase BMD. Body exercise was recommended to increase BMD as well.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.522-525
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• 2018

In this paper, we design a UWB / WAS microstrip antenna for wireless local high - speed communication. The substrate of the proposed antenna is FR-4 (er = 4.3) and its size is $30[mm]{\times}30[mm]$. It is designed to have characteristics that can be used in the frequency bands of 3.5 [GHz] and 5.2 [GHz], which are UWB / WAS frequency bands. The simulation was performed using CST Microwave Studio 2014. Simulation results show that the gain is 1.592 [dBi] at 3.5 [GHz] and 2.210 [dBi] at 5.2 [GHz]. The S-parameter also showed a result of less than -10 [dB] (WSWR 2: 1) in the desired frequency band. Microstrip antennas have been miniaturized, high performance, and light weight, and excellent and low cost systems are continuously being developed. In addition, many people use wireless local area network systems used in homes, companies, and public facilities. Since the UWB / WAS technology is proposed according to the development of the system and the demand increase, the antenna that satisfies the above conditions will be designed and the technology applicable to the system will be used more conveniently.

• Korean Journal of Construction Engineering and Management
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• v.14 no.2
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• pp.3-11
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• 2013

Recently according to increasement of the single family and dual-earner couples, the number of infants and young children stays on a long time to the childcare facilities. this is increasing the importance to improve the physical environment. However, the operating and maintenance costs of the national-public childcare facilities have taken effect of the unfair support issues of municipality's financial situation. Especially, because of the lack of the operating and maintenance costs of the facility and the shortage of facilities equipment maintenance repair costs, nursery operations and Maintenance items are needed to distribute appropriately. Therefore, this study is to estimate the importance to facility operations and maintenance items based on "Kindergarten and Childcare Facilities"presented at the Child Care Policy Study. we are expected to allocate appropriately of operation and maintenance cost of a limited budget. In particular, those can be considered for operation and maintenance cost about the National-Public Childcare Facilities and be determined to a safe and pleasant environment to kindergartens through the appropriate operations and maintenance support.

• Korean journal of communication and information
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• v.75
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• pp.43-79
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• 2016

An increase in the number of immigrants to Korea since the late 1980s' has signified the proliferation of globalization and global capitalism. In Korea, most married immigrants are women, as the culture emphasizes patrilineage and the stability of the institution of marriage, particularly in rural areas. Immigrant women have experienced dual ordeals. The Aogra Internet community in Korea has been one of the most representative sites that has shown the power of communities in cyberspace since 2002, leading the discussion of social issues and deliberative democracy both online and offline. This paper analyzed Koreans' writings (such as long comments) on immigrant women in the Agora community. The analysis revealed the following results: first, immigrant women were referred to using terms related to prostitution, with excessive expression of disgust, which is called a "narrative of identity." Second, anti-multiculturalists called Korean men victims of married immigrant women and expressed hatred toward immigrant women, which is called a "narrative of sacrifice." Third, anti-multiculturalists justified their emotions as just resentment based on ideas of justice, equality, and patriotism, concealing the emotion of disgust, which is called the "narrative of justice, equality." Fourth, antimulticulturalists played roles to spread the emotion of disgust, by repeatedly referring to international marriage fraud and immigrant workers' crimes, which is called "narrative of crime." Fifth, some positive writings on immigrant women were based on empathy(a concept defined in this context by Martha Nussbaum), but they can be analyzed as narratives encouraging cultural integration through the perspective of orientalism. Therefore, comments on immigrant women in the Agora represent a "catch-22" dilemma. To deal with conflicts arising from disgust and violations of human rights, civic education focusing on humanism is needed in this multicultural era.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.1
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• pp.9-17
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• 2013

In this study, an efficient analytical model for the dynamic analysis of tall buildings with a shear wall-frame structural system has been proposed. A shear wall-frame structural system usually consists of a core wall showing flexural behavior and a frame presenting shear behavior. Therefore, the deformed shape of the shear wall-frame structural system is shown by the combination of flexural mode and shear mode. These characteristics should be considered when an efficient analytical model is developed. To this end, the effect of shear wall and frame on the dynamic behavior of a tall building with a dual system has been separately investigated. In this study, the structural characteristics of a separated individual shear wall model and the frame model without shear wall has been evaluated. In order to consider the effect of the shear wall in the frame model without shear wall, a rigid body was used instead of the shear wall. Each equivalent model for the separated shear wall part and frame part has been independently developed and two equivalent models were then combined to create an efficient analytical model for tall buildings with a shear wall-frame structural system. In order to verify the efficiency and accuracy of the proposed method, time history analyses of tall buildings with a shear wall-frame system were performed. Based on analytical results, it has been confirmed that the proposed method can provide accurate results, requiring significantly reduced computational time and memory.

• The Korean Journal of Malacology
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• v.26 no.2
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• pp.185-188
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• 2010

The Web-based the genus Haliotis SNP database was constructed on the basis of Intel Server Platform ZSS130 dual Xeon 3.2 GHz cpu and Linux-based (Cent OS) operating system. Haliotis related sequences (2,830 nucleotide sequences, 9,102 EST sequences) were downloaded through NCBI taxonomy browser. In order to eliminate vector sequences, we conducted vector masking step using cross match software with vector sequence database. In addition, poly-A tails were removed using Trimmest software from EMBOSS package. The processed sequences were clustered and assembled by TGICL package (TIGR tools) equipped with CAP3 software. A web-based interface (Haliotis SNP Database, http://www.haliotis.or.kr) was developed to enable optimal use of the clustered assemblies. The Clustering Res. menu shows the contig sequences from the clustering, the alignment results and sequences from each cluster. And also we can compare any sequences with Haliotis related sequences in BLAST menu. The search menu is equipped with its own search engine so that it is possible to search all of the information in the database using the name of a gene, accession number and/or species name. Taken together, the Web-based SNP database for Haliotis will be valuable to develop SNPs of Haliotis in the future.