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Expression and Promoter Analyses of Pepper CaCDPK4 (Capsicum annuum calcium dependent protein kinase 4) during Plant Defense Response to Incompatible Pathogen

  • Chung, Eun-Sook;Oh, Sang-Keun;Park, Jeong-Mee;Choi, Do-Il
    • The Plant Pathology Journal
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    • v.23 no.2
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    • pp.76-89
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    • 2007
  • CaCDPK4, a full-length cDNA clone encoding Capsicum annuum calcium-dependent protein kinase 4, was isolated from chili pepper (Capsicum annuum L.). Deduced amino acid sequence of CaCDPK4 shares the highest homology with tobacco NpCDPK8 and chickpea CaCDPK2 with 79% identity. Genomic blot analyses revealed that CaCDPK4 is present as a single copy in pepper genome, but it belongs to a multigene family. CaCDPK4 was highly induced when pepper plants were inoculated with an incompatible bacterial pathogen. Induced levels of CaCDPK4 transcripts were also detected in pepper leaves by the treatment of ethephon, an ethylene-inducing agent, and high-salt stress condition. The bacterial-expressed GST-CaCDPK4 protein showed to retain the autophosphorylation activity in vitro. GUS expression driven by CaCDPK4 promoter was examined in transgenic Arabidopsis containing transcriptional fusion of CaCDPK4 promoter. GUS expression under CaCDPK4 promoter was strong in the root and veins of the seedlings. GW (-1965) and D3 (-1377) promoters conferred on GUS expression in response to inoculation of an incompatible bacterial pathogen, but D4-GUS (-913) and DS-GUS (-833) did not. Taken together, our results suggest that CaCDPK4 can be implicated on signal transduction pathway of defense response against an incompatible bacterial pathogen in pepper.

Effects of Feeding Licury (Syagrus coronate) Cake to Growing Goats

  • Borja, M.S.;Oliveira, R.L.;Ribeiro, C.V.D.M.;Bagaldo, A.R.;Carvalho, G.G.P.;Silva, T.M.;Lima, L.S.;Barbosa, L.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.11
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    • pp.1436-1444
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    • 2010
  • The objectives of this study were to determine the highest inclusion of licury (Syagrus coronate) cake in the diet of growing Boer goats without adverse effects on intake and digestibility and to determine its effects on ingestive behavior and physiological responses. Twenty entire, one year old 3/4 Boer goats, 18.1 kg (DS = 2.2) average body weight (BW), were allocated to dietary treatments in a completely randomized design. Each animal was confined in a $1.0\;m^2$ pen with a suspended floor and given ad libitum access to clean, fresh water. Diets were formulated to meet NRC (2007) requirements and the ingredients were: 50% of Tifton-85 (Cynodon sp.) hay, corn meal, soybean meal, mineral and vitamin premix, and licury cake. The treatments were: i) no addition of licury cake to the diet, ii) 15% (DM basis) addition of licury cake, iii) 30% licury cake and, iv) 45% licury cake. The experiment lasted for 17 days; the first 10 days were used to adapt the animals to the diets and facilities. The inclusion of licury cake increased the fiber concentration of the diets; however, there was no effect on either dry matter (DM) or organic matter (OM) intake. There was a linear increase (p<0.05) in the EE content of the diet as the addition of licury cake increased; however, EE intake did not differ (p>0.05) between treatments. The digestibility of non-fibrous carbohydrates (NFC) decreased with increasing inclusion of licury cake, as did NFC intake. The efficiency of ingestion of DM and NDF presented a negative quadratic effect with the inclusion of licury cake. Results from this study indicate that licury cake can be fed to goats at up to 45% of the diet without adverse effects on either intake or digestibility.

A Study on the Modeling of Three-Dimensional Work Structure Using Web 3D in Cyberspace (Cyberspace에서 Web 3D를 이용한 3차원 공사 구조물 모델링의 활용성 제언)

  • Lee Hui-Chul;Lee Tai-Sik;Kim Young-Hyun
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • autumn
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    • pp.531-534
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    • 2002
  • Complexity in construction projects has increased over the years, which has led to an increase in the number of organizations involved in those projects. In today's environment, these organizations operate in different parts of the world requiring their personnel to be geographically distributed. So, these organizations construct and utilize the information systems. However, looking into this systems' content, those use two-dimensional planes such as pictures and drawings to check the scheduling etc., which cause the defect of communication and problems such as change order. Accordingly, this study introduces the Web 3Ds and 3D Libraries, intends to present the effect on modeling of three-dimensional work structure using Web 3D in cyberspace.

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The Improvement of High Convergence Speed using LMS Algorithm of Data-Recycling Adaptive Transversal Filter in Direct Sequence Spread Spectrum (직접순차 확산 스펙트럼 시스템에서 데이터 재순환 적응 횡단선 필터의 LMS 알고리즘을 이용한 고속 수렴 속도 개선)

  • Kim, Gwang-Jun;Yoon, Chan-Ho;Kim, Chun-Suk
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.1
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    • pp.22-33
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    • 2005
  • In this paper, an efficient signal interference control technique to improve the high convergence speed of LMS algorithms is introduced in the adaptive transversal filter of DS/SS. The convergence characteristics of the proposed algorithm, whose coefficients are multiply adapted in a symbol time period by recycling the received data, is analyzed to prove theoretically the improvement of high convergence speed. According as the step-size parameter ${\mu}$ is increased, the rate of convergence of the algorithm is controlled. Also, an increase in the stop-size parameter ${\mu}$ has the effect of reducing the variation in the experimentally computed learning curve. Increasing the eigenvalue spread has the effect of controlling which is downed the rate of convergence of the adaptive equalizer. Increasing the steady-state value of the average squared error, proposed algorithm also demonstrate the superiority of signal interference control to the filter algorithm increasing convergence speed by (B+1) times due to the data-recycling LMS technique.

NMR peak assignment for the elucidation of the solution structure of T4 Endonuclease V

  • Im, Hoo-Kang;Hyungmi Lihm;Yu, Jun-Suk;Lee, Bong-Jin
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.183-183
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    • 1996
  • Bacteriophage T4 endonuclease V initiates the repair of ultraviolet (UV)-induced pyrimidine dimer photoproducts in duplex DNA. The mechanism of DNA strand cleavage involves four sequential stens: linear diffusion along dsDNA, pyrimidine dimer-specific binding,l pyrimidine dimer-DNA glycosylase activity, and Af lyase activity. Although crystal structure is known for this enzyme, solution structure has not been yet known. In order to elucidate the solution structure of this enzyme NMR spectroscopy was used. As a basis for the NMR peak assignment of the protein, HSQC spectrum was obtained on the uniformly $\^$15/N-labeled T4 endonuclease V. Each amide peak of the spectrum were classified according to amino acid spin systems by interpreting the spectrum of $\^$15/N amino acid-specific labeled T4 endonuclease V. The assignment was mainly obtained from three-dimensional NMR spectra such as 3D NOESY-HMQC, 3D TOCSY-HMQC. These experiments were carried out will uniformly $\^$15/N-labeled sample. In order to assign tile resonance of backbon atom, triple-resonance theree-dimensional NMR experiments were also performed using double labeled($\^$15/N$\^$13/C) sample. 3D HNCA, HN(CO)CA, HNCO, HN(CA)HA spectra were recorded for this purpose. The results of assignments were used to interpret the interaction of this enzyme with DNA. HSQC spectrum was obtained for T4 endonuclease V with specific $\^$15/N-labeled amino acids that have been known for important residue in catalysis. By comparing the spectrum of enzyme*DNA complex with that of the enzyme, we could confirm the important role of some residues of Thr, Arg, Tyr in activity. The results of assignments were also used to predict the secondary structure by chemical shift index (CSI).

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Electrical Characteristics of Tunneling Field-effect Transistors using Vertical Tunneling Operation Based on AlGaSb/InGaAs

  • Kim, Bo Gyeong;Kwon, Ra Hee;Seo, Jae Hwa;Yoon, Young Jun;Jang, Young In;Cho, Min Su;Lee, Jung-Hee;Cho, Seongjae;Kang, In Man
    • Journal of Electrical Engineering and Technology
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    • v.12 no.6
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    • pp.2324-2332
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    • 2017
  • This paper presents the electrical performances of novel AlGaSb/InGaAs heterojunction-based vertical-tunneling field-effect transistor (VTFET). The device performance was investigated in views of the on-state current ($I_{on}$), drain-induced barrier thinning (DIBT), and subthreshold swing (SS) as the gate length ($L_G$) was scaled down. The proposed TFET with a $L_G$ of 5 nm operated with an $I_{on}$ of $1.3mA/{\mu}m$, a DIBT of 40 mV/V, and an SS of 23 mV/dec at a drain voltage ($V_{DS}$) of 0.23 V. The proposed TFET provided approximately 25 times lower DIBT and 12 times smaller SS compared with the conventional $L_G$ of 5 nm TFET. The AlGaSb/InGaAs VTFET showed extremely high scalability and strong immunity against short-channel effects.

Design Procedure of Stress Ribbon Pedestrian Bridges (스트레스 리본 보도교의 설계절차)

  • Han, Ki-Jang;Choi, Young-Goo;Park, Kyoung-Yong;Kim, Kee-Dong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.5
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    • pp.2474-2480
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    • 2013
  • A stress ribbon pedestrian bridge is the structure in which the axial force of prestressed deck, which is developed by introducing prestressed force into the thin deck with the very low value of span to deck-depth ratio which is installed on bearing cables with the specified sag, resists most of external loadings. Since the design of stress ribbon pedestrian bridges should be conducted by assuming the cross-section of deck, the area of bearing cables and post-tensioning cables, and the prestressed force of post-tensioning cables, it requires much more iterative processes than the design of general bridges. In this research, to minimize such iteration processes, regression equations which can reasonably assume the area of bearing cables and post-tensioning cables, and the prestressed force of post-tensioning cables, are suggested for the bridge length of 80m with the sag-span ratios of 1/30, 1/40, and 1/50.

A Distributed Bandwidth-Interference aware CDS (BI CDS) Construction Scheme in Wireless Mesh Network (무선 메쉬 네트워크에서 최소 간섭과 최적의 주파수 대역폭을 이용한 CDS 구성)

  • Yang, Jun-Mo;Kim, Bo-Nam;Kim, Heung-Jun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.1
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    • pp.53-59
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    • 2009
  • As a special type of Wireless Ad Hoc Networks, Wireless Mesh Networks (WMNs) have become the comer stone of research issues. Due to the limited operational environment of WMNs, an efficient connected dominating set (CDS) construction scheme is an important concern since it has been found extremely useful in broadcasting, routing and virtual backbone construction. In this paper, we propose a distributed Bandwidth-Interference aware CDS construction scheme to improve the network performance via two parameters such as node's number of neighbor and link bandwidth. Our CDS construction scheme selects the node that has more neighbors and enough bandwidth to support more end-devices in order to enhance overall network throughput and reliability. We confirm through simulations and show that our scheme constructs the CDS with the small subset of DS and the link that has better bandwidth.

Eigen-Analysis Based Super-Resolution Time Delay Estimation Algorithms for Spread Spectrum Signals (대역 확산 신호를 위한 고유치 해석 기반의 초 분해능 지연 시간 추정 알고리즘)

  • Park, Hyung-Rae;Shin, Joon-Ho
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.12
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    • pp.1013-1020
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    • 2013
  • In this paper the super-resolution time delay estimation algorithms based on eigen-analysis are developed for spread spectrum signals along with their comparative performance analysis. First, we shall develop super-resolution time delay estimation algorithms using the representative eigen-analysis based AOA (angle-of-arrival) estimation algorithms such as MUSIC, Minimum-Norm, and ESPRIT, and apply them to the ISO/IEC 24730-2.1 real-time locating system (RTLS) employing a direct sequence spread spectrum (DS-SS) technique to compare their performances in RTLS environments. Simulation results illustrate that all the three algorithms can resolve multipath signals whose delay differences are even smaller than the Rayleigh resolution limit. Simulation results also show that MUSIC and Minimum-Norm provide a similar performance while ESPRIT is inferior to both algorithms in RTLS environments.

Optimal Information Dispersal Scheme for Survivable Storage Systems (서바이벌 스토리지 시스템을 위한 최적 정보 분할 기법)

  • Song, Sung-Keun;Youn, Hee-Yong;Lee, Hyung-Soo;Lee, Kang-Shin
    • The KIPS Transactions:PartA
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    • v.10A no.6
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    • pp.657-664
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    • 2003
  • Supporting the availability, integrity, and confidentiality of the information is crucial. The survivability storage systems require to encode and distribute data over multiple storage nodes or data base to survive failures and malicious attacks Information dispersal scheme is one of the most efficient schemes allowing high availability and security with reasonable overhead. In this paper, we propose an algorithm determining the optimal (m, n)-lDS in terms of availability, given a set of IDS's. The proposed algorithm will be very useful for designing a highly available and secure storage system since many factors such as node number, storage space, operation speed, etc. interact with each other and thereby finding an optimal information dispersal scheme is very difficult.