• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.55-61
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• 2011

Forty yeast strains isolated from soils taken from different locations in Egypt were tested for their P-solubilizing activities on the basis of analyzing the clear zone around colonies growing on a tricalcium phosphate medium after incubation for 5 days at $25^{\circ}C$, denoted as the solubilization index (SI). Nine isolates that exhibited P-solubilization potential with an SI ranging from 1.19 to 2.76 were genetically characterized as five yeasts belonging to the genus Saccharomyces cerevisiae and four non-Saccharomyces, based on a PCR analysis of the ITS1-26S region amplied by SC1/SC2 species-specific primers. The highest P-solubilization efficiency was demonstrated by isolate PSY- 4, which was identified as Saccharomyces cerevisiae by a sequence analysis of the variable D1/D2 domain of the 26S rDNA. The effects of single and mixed inoculations with yeast PSY-4 and Bacillus polymyxa on the P-uptake and growth of corn were tested in a greenhouse experiment using different levels of a phosphorus chemical fertilizer (50, 100, and 200 kg/ha super phosphate 15.5% $P_2O_5$). The results showed that inoculating the corn with yeast PSY-4 or B. polymyxa caused significant increases in the shoot and root dry weights and P-uptake in the shoots and roots. The P-fertilization level also had a significant influence on the shoot and root dry weights and P-uptake in the shoots and roots when increasing the P-level from 50 up to 200 kg/ha. Dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 200 kg/ha gave higher values for the shoot and root dry weights and P-uptake in the shoots and roots, yet these increases were nonsignificant when compared with dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha. The best increases were obtained from dual inoculation with yeast strain PSY-4 and B. polymyxa at a P-fertilization level of 100 kg/ha, which induced the following percentage increases in the shoot and root dry weights, and P-uptake in the shoots and roots; 16.22%, 46.92%, 10.09%, and 31.07%, respectively, when compared with the uninoculated control (fertilized with 100 kg/ha).

• 한국초지조사료학회지
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• 제15권1호
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• pp.30-36
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• 1995

Pasture composed of about 30% botanical composition of white clover (Trijolium repem L.) is desirable in its productivity and quality. To get information on maintaining its productivity in the pasture, the experiment was canied out to determine the effect of infrequent, frequent defoliation or their alternation on change of its harvest yield and growth. Individual plants of Regal, Louisiana S-1, Grasslands Huia and Aberystwyth S184 were grown in 22cm plastic pot containing a 2 : 1 : 1 soil : sand : Peat moss mixture for 27 days after transplanting 50day seedlings raised on 3cm pots, and then all their fully expanded leaves are removed. Defoliation treatments were forced every 1 (CC, frequent), 4 week (RR, infrequent) or their alternations (CR, RC) after 8 weeks from the removal. To analyze the treatment effects, plants were sampled on 0 (the removal day), 4, 8, 12 and 16 weeks after the removal and seperated to leaves, petioles, stolons and roots. Each harvest yield of infrequent defoliation (RR) was higher than that of frequent defoliation (CC). Their alternations (RC, CR), however, forced fluctuation of the yield according to defoliation interval. Fraction weights from their altemations showed intermediate ones of infrequent and Frequent defoliation whose weights did the similar result to their harvest yields. Fraction and total dry weights per plant, shoot/root ratio were changed by relative span of defoliation interval, which resulted from the weights of leaves and petioles, removed parts. Root and total dry weight per plant of Regal were greater than the other cultivm in infrequent defoliation interval regardless of continuous or alternations. Productivity of white clover in pasture, therefore, can be controlled by alternation of infrequent and frequent defoliation or reverse.

• 한국초지조사료학회지
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• 제14권2호
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• pp.120-124
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• 1994

Browse from oriental white oak(Quercus aliena Blume) was analysed for nutritive value and biomass estimation. The content of cmde protein was high on August and September. NDF, ADF and lignin contents were increased with progressing the seasons but IVDMD, TDN, DE and ME levels were not different from the variation of seasons. Tannin content was high on May and June. Basal stem diameter $\times$ height of tress was highly correlated with browse dry weights(r=0.80**) and the linear regression equation are the follows; Y = 8.23 + O.57X(Y = browse dry weight(g), X= basal stem diameter $\times$ height(cm)). Using the traditional cutting method, the amounts of browse was determined as 823 g dry weight per tree, while the new method by the above equation gave a similar results, i. e., 793 g dry weight per tree. The possibility of browse biomass estimation of oriental white oak by the basal stem diameter $\times$ height index was found.

• 한국작물학회지
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• 제37권4호
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• pp.339-346
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• 1992

콩 품종간의 상호접목을 통하여 지상부와 지하부의 능력을 비교하여 콩품종개량이나 재배법 개선의 기초자료를 제공하고자 황금콩, 팔달콩, 보광콩 및 장경콩을 공시하여 자가접목 및 품종간 상호접목을 하여 이식재배한 결과를 요약하면 다음과 같다. 1. 보광콩의 초장이 106.3-109.8cm로 가장 컸으며 팔달콩이 68.6-70.3cm로 제일 작았는데, 접목에 의하여 초장이 감소되기는 하였지만 대목의 영향은 인정되지 않았다. 2. 엽수는 팔달콩이 9.6-9.8매로 가장 적었으며, 장경콩이 14.2-15.3매로 제일 많았는데 처리간의 차이는 없었다. 3. 개화일수는 접목에 의하여 2-4일 지연되어 유의한 차이를 보였는데, 황금콩과 팔달콩은 자가접목보다는 상호접목에서 지연정도가 켰으나 보광콩과 장경콩은 상호접목보다는 자가접목에서 지연정도가 더 커서 품종간에 접목에 대한 반응이 다른 것으로 나타났다. 4. 부위별 건물중에 대한 접목의 영향도 품종간에 유의한 차이를 보여 황금콩과 팔달콩에서는 엽중에 대한 접목의 영향이 인정되었고, 보광콩과 장경콩의 상호접목에서도 장경콩이 보광콩의 부위별 건물중을 유의하게 감소시킨 것으로 나타났다. 5. 경태는 접목에 의하여 증가하였으며, 분지수는 감소하였고, 보광콩은 접목에 의하여 증중이 감소하였다. 6. 수양은 자가접목이 비접목에 비하여 11%, 상호접목은 자가접목에 비하여 14%그리고 상호접목은 비접목에 비하여 24% 감소하였으며, 경중은 수양과 같은 경향이었으나 접목에 의한 감소가 29-32%로 정도가 수량에 비하여 더 켰으나 자가접목과 상호접목간의 차이는 거의 없었다. 7 .수확지수는 접목에 의하여 황금콩과 장경콩은 1.4-6.1% 그리고 팔달콩과 보광콩은 4.4-8.6% 높아졌다. 8. 접목하였을 때의 수양이나 경중은 대목보다는 접수로 사용된 품종의 유전적 특성에 의하여 좌우되었으며, 품종과 특성에 따라서 접목에 대한 반응의 차이가 인정되었다.

• 한국초지조사료학회지
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• 제14권3호
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• pp.177-185
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• 1994

Slow seedling growth rate and nodulation failure of white clover (Trifolium repens L.) has been limited its good establishment to pastures. The experiment was done to determine the effect of removal of cotyledon and unifoliolate on the shoot, root growth, and nodule formation of 4 white clover cultivars for 8 weeks after the treatment. Four white clover cv. Regal (large leaf), Louisiana S-I (medium-large leaf), Grasslands Huia (mediumsmall leaf), and Aberystwyth S184 (small leaf), were grown in IOcm plastic pot containing 2:l:l soi1:sand:peat moss mixture until grown to cotyledon or unifoliolate stage and then removed one (Cl) or two cotyledons (C2) at cotyledon stage, and unifoliolate only (U), unifoliolate and one cotyledon (UCl) or unifoliolate and two cotyledons (UC2) at the unifoliolate stage, and the plants were sampled at 4, 6 and 8 weeks after the treatments. The intact plants had greater shoot and root dry weights, and no. of nodules than removal-treated ones. Removal treatments at cotyledon stage, the dry weight and no. of nodules more decreased in C1 and C2 than that of unifoliolate stage. While the severer cotyledon removal, the more reduction. Although the dry weights and no. of nodules steadily inclined with regrowing period, the former were higher in Regal and La. S-1 than in the others since 6 weeks after removal treatment but the latter was more in S 184 than in the others 8 weeks after removal treatment. Relationship between no. of nodules and shoot or root dry weight was analysed as linear mode while the earlier and severe removal, the steeper slope. It was concluded that severer damage of cotyledon and unifoliolate had detrimental effects on the shoot and root growth, nodule formation, and aftermath establishment of white clover.

• 생물환경조절학회지
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• 제15권1호
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• pp.8-20
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• 2006

This research was conducted to determine the plant growth and nutrient contents of potted chrysanthemum 'Lima Honey' as influenced by application rate of dolomite in three root media, peatmoss+vermiculite (1:1, v/v), peatmoss+composted rice hall (1:1, v/v), and peatmoss+composted pine bark (1:1, v/v). All root media contained polyacrylic acid sodium salt (PASS) at a rate of $4.5g L^{-1}$. In crop growth at 40 and 80 days after transplanting, elevated application rates of dolomite up to $6.0g L^{-1}$ in three root media increased fresh and dry weights per plant. But the treatment of $9.0g L^{-1}$ had less fresh and dry weights than those of $6.0g L^{-1}$. Tissue phosphorus content decreased and those of Ca and Mg increased by the elevated application rates of dolomite. Elevated application rates of dolomite in three root media resulted in the increase of pH, EC, and the concentrations of K, Ca, and Mg at 43 days after transplanting. Those at 80 days after transplanting were also increased, but the differences among treatments were less significant as compared to those at 43 days after transplanting. From the results of dry weight in potted chrysanthemum 'Lima Honey', the proper application rate of dolomite to three root media containing PASS at a rate of $4.5g L^{-1}$ were $6.0g L^{-1}$.

• 한국작물학회지
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• 제45권2호
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• pp.88-92
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• 2000

Excessive soil water in paddy field induces growth losses during the vegetative stages of soybean plants. Our objectives were to know growth responses of soybean as affected by the level of ridge heights. A field experiment was conducted at the Research Farm of Korea University near Seoul in 1996. Ten cultivars of soybean (Glycine max (L.) Merrill) were planted at the ridge heights of 10, 30, and 50cm in paddy soil on 27 June. Total dry matter accumulation at the 10cm ridge height was severely decreased until the growth stage of R5 as compared to the ridge heights of 30 and 50cm, and this decrement was mainly due to both reduced leaf and stem dry weights. However, seed dry weight was not significantly decreased at the lower ridge height with the increment of harvest index. Cultivar differences were found on seed dry weight, crop growth rate from R1 to R5, and leaf/stem ratio of R5. From the above results, soybean growth responses to excessive soil water in paddy field were different between seed filling stage and late vegetative to early reproductive growth stages.

• 한국축산식품학회지
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• 제23권4호
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• pp.315-320
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• 2003

A total of 240 crossbred(Landrace ${\times}$ Yorkshire ${\times}$ Duroc) pigs were housed from 70 kg live weight and slaughtered at weights of 95, 105, 115 and 125 kg. The left side loins of carcass were obtained at 24 hr postmortem to measure pork quality. There were significant differences(p<0.01) in eye muscle area of pork loins between the slaughter weights of 95 and 105 kg. However, no differences were observed in pork than 105 kg of slaughter weight. Ultimate pH values were decreased with increasing slaughter weight and cooking loss was also reduced(p<0.01) at the heavier weights. Slaughter weights did not affect the shear force and intramuscular fat. However, dry matter(DM) and crude protein(CP) contents of loin were increased, and cooking loss and sarcomere length were decreased with increasing slaughter weight. The lightness(L$^{*}$) and redness(a$^{*}$) of pork loin were increased with increasing slaughter weight. Results suggested that pork quality may be improved when pig slaughter weight is increased from 95 to 125 kg.

• 대한예방한의학회지
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• 제18권2호
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• pp.133-145
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• 2014

Objective : In this study, the addition of dried pomegranate concentrate powder (PCP) was affected the anti-climacterium activity of red clover dry extracts (RC) in ovariectomized (OVX) rats. Materials and methods : After bilateral OVX surgery, RC 40 mg/kg, PCP 20 mg/kg and RC:PCP 2:1 mixture (g/g) 120, 60 and 30 mg/kg (of body weight) were orally administered, once a day for 84 days, and then the changes on the serum estradiol levels, abdominal fat pad and uterus weights were observed for estrogenic effects. In addition, liver weights, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were also evaluated for hepatoprotective effects, and serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglyceride (TG) levels were monitored for hypolipidemic effects. Results : As a result of OVX, the estrogen-deficient climacterium symptoms, increments of abdominal fat pad weights, serum AST, ALT, TC, LDL and TG levels with decrease of uterus and liver weights, serum estradiol levels, were demonstrated. However, these estrogen-deficient climacterium symptoms induced by bilateral OVX in rats were significantly inhibited by continuous oral treatment of RC 40 mg/kg, PCP 20 mg/kg and RC:PCP 2:1 mixture (g/g) 120, 60 and 30 mg/kg, respectively. Conclusion : The results suggested that RC:PCP 2:1 mixtures synergistically increased the anti-climacterium effects of RC in OVX rats. It, therefore, is expected that RC:PCP 2:1 mixture will be promising as a new potent protective agents for relieving the climacterium symptoms.

• 농업생명과학연구
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• 제43권1호
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• pp.1-8
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• 2009

본 연구의 목적은 강원도 강릉시 강릉국유림 관리소 관할의 국유림 내에 위치한 27년생 잣나무 조림지를 대상으로 하여 바이오매스 및 바이오매스 구성비, 바이오매스 추정식, 순생산량, 줄기밀도 및 바이오매스 확장계수 등을 조사 분석하는데 있다. 선정된 표준지에 대하여 $20m{\times}20m$ 조사구를 설치한 후 매목조사를 실시하고, 직경급 분포를 고려하여 총 5주의 표본목을 벌채하였으며, 이 중 3주는 뿌리까지 전량 굴취하였다. 27년생 잣나무에 대한 임목 전체 건중량은 117.6 kg/tree로 나타났으며, 잣나무 임분의 지상부 건중량은 59.9 ton/ha, 지하부를 포함한 임목 전체 건중량은 82.4 ton/ha로 나타났다. 뿌리 대 지상부 비율은 0.38이였으며, 순생산량은 지상부 건중량 9.4 ton/ha, 지하부를 포함한 임목 전체 건중량 11.3 ton/ha로 나타났다. 줄기밀도는 $0.49g/cm^{3}$, 지상부 바이오매스 확장계수는1.78, 지상부와 지하부를 포함한 임목 전체 바이오매스 확장계수는 2.19로 나타났다. 이와 같은 자료는 잣나무 조림지에 대하여 바이오매스 및 탄소축적량을 추정하는데 활용될 수가 있다.