• Title/Summary/Keyword: Dry spot

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Development of DNA Chip Microarrayer

  • Yoon, Sung-Ho;Choi, Jong-Gil;Lee, Sang-Yup
    • Journal of Microbiology and Biotechnology
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    • v.10 no.1
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    • pp.21-26
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    • 2000
  • A microarrayer system was developed mainly for manufacturing DNA chips. The 3-axis robot was designed to automatically collect samples from 96-or 384-well microtiter plates using up to 16 simultaneously moving pens and to deposit them on a surface-modified slide glass. This is followed by a wash/dry operation in a clean station. The cycle is repeated with a new set of samples, This system can deposit cDNA or oligonucleotides with spot intervals of $150{\;}\mu\textrm{m}$ and the spot size of $80\mu\textrm{m}$, thus allowing a high density DNA chip containing about 5,000 spots per $\textrm{cm}^2$. The entire procedure is controlled by the Visual C++ program that was written in our laboratory by using a personal computer with Pentium 100 CPU.

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Morphological and Molecular Characterization of Pseudocercospora chionanthi-retusi Causing Leaf Spot on Chionanthus retusus in Korea

  • Choi, In-Young;Abasova, Lamiya;Choi, Joon-Ho;Shin, Hyeon-Dong
    • Research in Plant Disease
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    • v.28 no.2
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    • pp.57-60
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    • 2022
  • Leaves of Chionanthus retusus were found to be damaged by leaf spot disease associated with a fungus in Iksan, Korea. Leaf spots were angular to irregular, vein-limited, scattered, 1-8 mm diameter, brownish-gray to dark brown when dry, with heavy fructification. The pathogen causes premature defoliation of C. retusus plant and was identified as Pseudocercospora chionanthi-retusi based on morphological and molecular-phylogenetic analyses. The phylogenetic tree was constructed using multi-locus DNA sequence data of partial actin (actA), partial translation elongation factor 1-alfa (tef1), partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes, and internal transcribed spacer regions. Current study provides detail morphological description of P. chionanthi-retusi on C. retusus in Korea, with supports of phylogenetic analysis and pathogenicity test.

Development of Coke Breeze Combustion Technology in the Calcining Rotary Kiln (Rotary Kiln 식석회소성로에서의 분코크스 연소 기술)

  • Kim, J.G.;Cho, H.C.;Kim, Y.W.
    • 한국연소학회:학술대회논문집
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    • 2004.11a
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    • pp.41-45
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    • 2004
  • A dust injection system was developed for the lime calcining rotary kiln for the coke dust from the coke dry quenching(CDQ) facility to be used as a fuel. The CDQ dust was injected with the gaseous fuel through the hole in the burner. In order to prevent the spot heating large particles should be removed from dust and dust should be injected as fast as possible so that particle combustion lasts as long as possible without precipitation. This is especially necessary when dust is burned together with gaseous fuel because the gaseous fuel can not go so far and in addition dust combustion aggravates hot spot heating. In this research a rotation drum screen was used to remove particles with diameter larger than 4mm and dust injection speed was 40m/sec. And the burner was adjusted not to use swirl that hinders flame go far away. With these measures scale generation iside the kiln could be reduced to be negligible and in addition NOx emission could be reduced from 150ppm to 20ppm. The fuel reduction was about 85Mcal/T-lime.

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Identification and Characterization of Pseudocercospora pyricola Causing Leaf Spots on Aronia melanocarpa

  • Park, Sung-Hee;Choi, In-Young;Seo, Kyoung-Won;Kim, Jin-Ho;Galea, Victor;Shin, Hyeon-Dong
    • Mycobiology
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    • v.45 no.1
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    • pp.39-43
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    • 2017
  • Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.

Bacterial Leaf Spot and Dry Rot of Lettuce Caused by Xanthomonas campestris pv. vitians (Xanthomonas campestris pv. vitians에 의한 상추 세균성점무늬병)

  • Lee, Seung-Don;Lee, Jung-Hee;Kim, Yong-Ki;Heu, Sung-Gi;Ra, Dong-Soo
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.66-70
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    • 2007
  • During 1997 and 1998, a new disease of lettuce (Lactuca sativa) was observed on greenhouse-grown plants in Kwangju and Kwangmyung. Lesions on leaves were irregular, small, pale green to black, water-soaked, and 2 to 5 mm in diameter, Coalescing lesions sometimes caused defoliation of older leaves. Isolations made from diseased leaves on yeast extract dextrose calcium carbonate agar yielded nearly pure cultures of a yellow pigmented bacterium typical of a xanthomonad. Two bacterial strains (SL0246 and SL1352) were purified and used for further tests. Pathogenicity of strains was confirmed on 5-week-old lettuce plants injected with bacterial suspensions containing $10^8$ cfu/ml of phosphate buffered saline. The representative Xanthomonas strains isolated from lettuce were compared with a reference strain X. campestris pv, vitians for fatty acid profiles and metabolic fingerprints using GN2 microplate, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot and dry rot of lettuce in Korea.

A Study on the Estimation of Adhesive Stability According to Organic.lnorganic Mixed Tile Bond Type for Application of Polishing Tile to Dry Wall System (건식벽체에 폴리싱타일을 적용하기 위한 유기.무기질 혼합계 타일접착제 종류에 따른 부착안정성 평가에 관한 연구)

  • Oh, Sang-Keun;Lee, Gi-Jang;Yoo, Jae-Kang;Kim, Su-Ryun;Lee, Sung-Il
    • Journal of the Korea Institute of Building Construction
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    • v.2 no.3
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    • pp.163-170
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    • 2002
  • Recently, polishing tile(porcelain homogeneous polished tile) was used in the construction field as a finishing material. But, there happened some problems such as tile exfoliation by construction condition in early ages. Also, for use of polishing tile in the dry wall system which used to lightweight wall, the examination of adhesive stability of polishing tile is needed. In this study, adhesive strength of Polishing tile was investigated by tile bond types on gypsum board and non asbestos board coated by tar-urethane and Polymer modified cementitious waterproofing membrane(Series I). Then, the effect of heat stress and vibration was estimated on gypsum and non asbestos board(Series II). As the result of study are the follows; (1) Polishing tile(600$\times$400mm) construction on waterproofing layer : Both laboratory estimation and spot examination sieve were happened that fall of tile because their hardening speed is late. (2) To using powder style adhesives in the dry wail with waterproofing layer : Adhesive strength of tile is Influenced by interface bond area and base side condition. (3) Shock and heat stresses : obvious decline of adhesive strength is not happened

Vegetation Water Status Monitoring around China and Mongolia Desert using Satellite Data (위성자료를 이용한 중국과 몽골 사막주변의 식생수분상태 모니터링)

  • Lee, Ga-Lam;Kim, Young-Seup;Han, Kyoung-Soo;Lee, Chang-Suk;Yeom, Jong-Min
    • Journal of the Korean Association of Geographic Information Studies
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    • v.11 no.4
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    • pp.94-100
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    • 2008
  • Recently, global warming for climate system is a crucial issue over the world and it brings about severe climate change, abnormal temperature, a downpour, a drought, and so on. Especially, a drought over the earth surface accelerates desertification which has been advanced over the several years mainly originated from a climatic change. The objective of this study is to detect variation of vegetation water condition around China and Mongolia desert by using satellite data having advantage in observing surface biological system. In this study, we use SPOT/VEGETATION satellite image to calculate NDWI (Normalized Difference Water Index) around study area desert for monitoring of status of vegetation characteristics. The vegetation water status index from remotely sensing data is related to desertification since dry vegetation is apt to desertify. We can infer vegetation water status using NDWI acquired by NIR (Near infrared) and SWIR (Short wave infrared) bands from SPOT/VGT. The consequence is that NDWI decreased around desert from 1999 to 2006. The areas that NDWI was decreased are located in the northeast of Mongolian Gobi desert and the southeast of China Taklamakan desert.

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Forecasting Bulk Freight Rates with Machine Learning Methods

  • Lim, Sangseop;Kim, Seokhun
    • Journal of the Korea Society of Computer and Information
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    • v.26 no.7
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    • pp.127-132
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    • 2021
  • This paper applies a machine learning model to forecasting freight rates in dry bulk and tanker markets with wavelet decomposition and empirical mode decomposition because they can refect both information scattered in the time and frequency domain. The decomposition with wavelet is outperformed for the dry bulk market, and EMD is the more proper model in the tanker market. This result provides market players with a practical short-term forecasting method. This study contributes to expanding a variety of predictive methodologies for one of the highly volatile markets. Furthermore, the proposed model is expected to improve the quality of decision-making in spot freight trading, which is the most frequent transaction in the shipping industry.

Factor Analysis Affecting on Chartering Decision-making in the Dry Bulk Shipping Market (부정기 건화물선 시장에서 용선 의사결정에 영향을 미치는 요인 분석)

  • Lee, Choong-Ho;Park, Keun-Sik
    • Journal of Korea Port Economic Association
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    • v.40 no.1
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    • pp.151-163
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    • 2024
  • This study sought to confirm the impact of analytical methods and behavioral economic theory factors on decision-making when making chartering decisions in the dry bulk shipping market. This study on chartering decision-making model was began to verify why shipping companies do not make rational decision-making and behavior based on analytical methods such as freight prediction and process of alternative selection in the same market situation. To understand the chartering decision-making model, it is necessary to study the impact of behavioral economic theory such as heuristics, loss aversion, and herding behavior on chartering decision-making. Through AHP analysis, the importance of the method factors relied upon in chartering decision-making. The dependence of the top factors in chartering decision-making was in the following order: market factors, heuristics, internal factors, herding behavior, and loss aversion. Market factors, heuristics, and internal factors. As for detailed factors, spot freight index and empirical intuition were confirmed as the most important factors relied on when making decisions. It was confirmed that empirical intuition is more important than internal analysis, which is an analytical method. This study can be said to be meaningful in that it academically researched and proved the bounded rationality of humans, which cannot be fully rational, and sometimes relies on experience or psychological tendencies, by applying it to the chartering decision-making model in the dry bulk shipping market. It also suggests that in the dry bulk shipping market, which is uncertain and has a high risk of loss due to decision-making, the experience and insight of decision makers have a very important impact on the performance and business profits of the operation part of shipping companies. Even though chartering are a decision-making field that requires judgment and intuition based on heuristics, decision-makers need to be aware of this decision-making model in order to reduce repeated mistakes of deciding contrary to market situation. It also suggests that there is a need to internally research analytical methods and procedures that can complement heuristics such as empirical intuition.