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Bacterial Leaf Spot and Dry Rot of Lettuce Caused by Xanthomonas campestris pv. vitians

Xanthomonas campestris pv. vitians에 의한 상추 세균성점무늬병

  • Lee, Seung-Don (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Lee, Jung-Hee (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Yong-Ki (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Heu, Sung-Gi (Plant Pathology Division, National Institute of Agricultural Science and Technology) ;
  • Ra, Dong-Soo (Plant Pathology Division, National Institute of Agricultural Science and Technology)
  • 이승돈 (농업과학기술원 식물병리과) ;
  • 이정희 (농업과학기술원 식물병리과) ;
  • 김용기 (농업과학기술원 식물병리과) ;
  • 허성기 (농업과학기술원 식물병리과) ;
  • 나동수 (농업과학기술원 식물병리과)
  • Published : 2007.04.01

Abstract

During 1997 and 1998, a new disease of lettuce (Lactuca sativa) was observed on greenhouse-grown plants in Kwangju and Kwangmyung. Lesions on leaves were irregular, small, pale green to black, water-soaked, and 2 to 5 mm in diameter, Coalescing lesions sometimes caused defoliation of older leaves. Isolations made from diseased leaves on yeast extract dextrose calcium carbonate agar yielded nearly pure cultures of a yellow pigmented bacterium typical of a xanthomonad. Two bacterial strains (SL0246 and SL1352) were purified and used for further tests. Pathogenicity of strains was confirmed on 5-week-old lettuce plants injected with bacterial suspensions containing $10^8$ cfu/ml of phosphate buffered saline. The representative Xanthomonas strains isolated from lettuce were compared with a reference strain X. campestris pv, vitians for fatty acid profiles and metabolic fingerprints using GN2 microplate, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot and dry rot of lettuce in Korea.

1997년과 1998년 광주와 광명의 상추 재배 온실에서 새로운 병해가 발견되었다. 처음에는 잎에 병반이 불규칙적이고 작은 연녹색이었다가 점점 커져 지름이 2$\sim$5 mm 정도인 수침상의 검은색 병반이 형성되었다. 잎의 아래쪽에서 엽맥을 따라 검은색의 줄무늬 병반이 형성되기도 하고 심해지면 잎이 시들어 말라 죽었다. YDC 배지에서 병원세균을 순수 분리하였을 때 Xanthomonas속 세균의 전형적인 특징인 노란색 색소를 띤 세균이 형성되었다. 분리된 세균을 $10^8$ cfu/ml로 현탁한 후 상추의 엽맥에 주사 접종하여 병원성을 확인하였다. 지방산 조성 및 함량과 다양한 탄소원 이용정도를 이용하여 분리세균 SL0246과 SL1352를 X. campestris pv. vitians로 동정하였으며 이 병을 상추의 세균성점무의병으로 명명하였다.

Keywords

References

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