• Journal of The Korean Society of Grassland and Forage Science
• /
• v.34 no.2
• /
• pp.129-140
• /
• 2014

This study was performed to evaluate the effects of replacing basic total mixed ration (TMR) with fermented soybean curd, Artemisia princeps Pampanini cv. Sajabal, and spent coffee grounds by-product on rumen microbial fermentation in vitro. Soybean in the basic TMR diet (control) was replaced by the following 9 treatments (3 replicates): maximum amounts of soybean curd (SC); fermented SC (FSC); 3, 5, and 10% FSC + fermented A. princeps Pampanini cv. Sajabal (1:1, DM basis, FSCS); and 3, 5, 10% FSC + fermented coffee meal (1:1, DM basis, FSCC) of soybean. FSC, FSCS, and FSCC were fermented using Lactobacillus acidophilus ATCC 496, Lactobacillus fermentum ATCC 1493, Lactobacillus plantarum KCTC 1048, and Lactobacillus casei IFO 3533. Replacing dairy cow TMR with FSC treatment led to a pH value of 6 after 8 h of incubation-the lowest value measured (p<0.05), and FSCS and FSCC treatments were higher than SC and FSC treatment after 6 h (p<0.05). Gas production was higher in response to 3% FSC and FSCC treatments than the control after 4-10 h. Dry matter digestibility was increased 0-12 h after FSC treatment (p<0.05) and was the highest after 24 h of 10% FSCS treatment. $NH_3-N$ concentration was the lowest after 24 h of FSC treatment (p<0.05). Microbial protein content increased in response to treatments that had been fermented by the Lactobacillus spp. compared to control and SC treatments (p<0.05). The total concentration of volatile fatty acids (VFAs) was increased after 6-12 h of FSC treatment (p<0.05), while the highest acetate proportion was observed 24 h after 5% and 10% FSCS treatments. The FSC of propionate proportion was increased for 0-10 h compared with among treatments (p<0.05). The highest acetate in the propionate ration was observed after 12 h of SC treatment and the lowest with FSCS 3% treatment after 24 h. Methane ($CH_4$) emulsion was lower with A. princeps Pampanini cv. Sajabal and spent coffee grounds treatments than with the control, SC, and FSC treatments. These experiments were designed to replace the by-products of dairy cow TMR with SC, FSC, FSCS, and FSCC to improve TMR quality. Condensed tannins contained in FSCS and FSCC treatments, which reduced $CH_4$ emulsion in vitro, decreased rumen microbial fermentation during the early incubation time. Therefore, future experiments are required to develop a rumen continuous culture system and an in vivo test to optimize the percentages of FSC, FSCS, and FSCC in the TMR diet of the dairy cows.

• Journal of Animal Science and Technology
• /
• v.52 no.5
• /
• pp.413-426
• /
• 2010

This study was conducted to investigate the effects on fermentation characteristics of rumen microorganism by different types and levels of lignosulfonate treated soybean meal (LSBM) in in vitro test and rumen simulation continuous culture (RSCC) system in dairy cows. The experiment I was control and 12 treatments (each with 3 replications) in vitro test to demonstrate composition of different types of treatments with lignosulfonate (Desulfonate, Na, Ca and solution) and levels (2, 4 and 8%) of soybean meal in the dairy cow diet. LSBM source treatments in the dairy cow diet showed pH value, $NH_3$-N concentration and total VFA concentration lower than control at all levels and incubation times (p<0.05). Dry matter digestibility of LSBM source treatments showed lower than control (p<0.05). Gas production and rumen microbial synthesis was decreased by rumen microbial fermentation for incubation times. Undegradable protein (UDP) concentration of all LSBM treatments was decreased for incubation times, and significantly higher than control (p<0.05). In the experiment II compared diets of the control, LSBM Na 2%, LSBM Sol 2%, which are high performance to undegradable protein (UDP) concentration experiment I in vitro test, and heated treatment lignosulfonate (LSBM Heat) 2% in the dairy cow diet from four station RSCC system ($4{\times}4$ Latin square). A rumen microbial fermentation characteristic was stability during 12~15 days of experimental period in all treatments. The pH value of LSBM treatments was higher than control treatment (p<0.05). The $NH_3$-N concentration, VFA concentration and rumen microbial synthesis of LSBM treatments were lower than control (p<0.05). The undegradable protein (UDP) showed LSBM Na 2% (45.28%), LSBM Sol 2% (43.52%) and LSBM Heat 2% (43.49%) higher than control (41.55%), respectively (p<0.05). Those experiments were designed to improve by-pass protein of diet and milk protein in the dairy cows. We will conduct those experiments the in vivo test by LSBM treatments in dairy cows diet.

• Korean Journal of Organic Agriculture
• /
• v.21 no.3
• /
• pp.451-465
• /
• 2013

This study was conducted to estimate Hanwoo carrying capacity when whole crop barley, rye, wheat and tritcale as winter forage crops was grown on different applying sources (chemical fertilizer, cattle or organic fertilizer) and mixed sowing combination with hairy vetch or forage pea during the period of 2011~2012. The experimental plots within whole crop barley or rye were consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (P+K), chemical fertilizer (N+P+K), organic fertilizer, cattle slurry, cattle slurry with hairy vetch, and cattle slurry with forage pea. Each plot was triplicates and experimental treatments were allocated in the randomized complete block design. For whole crop barley, annual mean dry matter (DM) and total digestible nutrients (TDN) yields were the highest in N+P+K plots, but there were no significant differences among organic fertilizer, cattle slurry and mixed sowing with legumes. The TDN were the highest in mixed sowing plots of forage pea plus cattle slurry application. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley, organic fertilizer, cattle slurry application and mixed sowing plots of forage pea is capable of raising average 2.8 to 3.1 heads/ha a year. For whole crop rye, annual mean DM were the highest in N+P+K plots, but there were no significant differences among cattle slurry. Organic fertilizer application significantly increased TDN and relative feed value (RFV) in comparison with treatments of N+P+K fertilization as chemical fertilizers. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 2.8~ 3.2 heads/ha a year. For whole crop wheat, annual DM, crude protein, and TDN yields of application groups and mixed sowing treatment with legumes showed 6.90~7.44, 0.53~0.60 and 4.35~5.04 ton/ha, respectively. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.1~3.7 heads/ha a year. For Triticale, TDN yield was significantly (P<0.05) higher N+P+K plots, organic ferilizer, cattle slurry, cattle slurry with legumes than for no fertilizer and N+P+K plots. The Crude protein (CP) contents were the highest in mixed sowing plots of forage pea plus cattle slurry application. In case of 450 kg Hanwoo heifers fed diets included 70% forage triticale, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.4~3.7 heads/ha a year. It can be concluded that, on the basis of DM yield, not only mixed sowing with legumes by applying cattle slurry rather than single sowing of whole crop barley or whole crop rye enhanced production yield and feed values, but also it could be a substitute for imported grains as dietary protein sources in the case of feeding Hanwoo.