Apical sealing is essential for the success of surgical endodontic treatment. Root-end cavity is apt to be contaminated with moisture or blood, and is not always easy to be dried completely. The purpose of this study was to evaluate the influence of dry methods of retrocavity on the apical seal in endodontic surgery. Apical seal was investigated through the evaluation of apical leakage and adaptation of filling material over the cavity wall. To investigate the influence of various dry methods on the apical leakage, 125 palatal roots of extracted human maxillary molar teeth were used. The clinical crown of each tooth was removed at 10 mm from the root apex using a slow-speed diamond saw and water spray. Root canals of the all the specimens were prepared with step-back technique and filled with gutta-percha by lateral condensation method. After removing of the coronal 2 mm of filling material, the access cavities were closed with Cavit$^{(R)}$. Two coats of nail polish were applied to the external surface of each root. Apical three millimeters of each root was resected perpendicular to the long axis of the root with a diamond saw. Class I retrograde cavities were prepared with ultrasonic instruments. Retrocavities were washed with physiologic saline solution and dried with various methods or contaminated with human blood. Retrocavities were filled either with IRM, Super EBA or composite resin. All the specimens were immersed in 2% methylene blue solution for 7 days in an incubator at $37^{\circ}C$. The teeth were dissolved in 14 ml of 35% nitric acid solution and the dye present within the root canal system was returned to solution. The leakage of dye was quantitatively measured via spectrophotometric method. The obtained data were analysed statistically using one-way ANOVA and Duncan's Multiple Range Test. To evaluate the influence of various dry methods on the adaptation of filling material over the cavity wall, 12 palatal roots of extracted human maxillary molar teeth were used. After all the roots were prepared and filled, and retrograde cavities were made and filled as above, roots were sectioned longitudinally. Filling-dentin interface of cut surfaces were examined by scanning electron microscope. The results were as follows: 1. Cavities dried with paper point or compressed air showed less leakage than those dried with cotton pellet in Super EBA filled cavity (p<0.05). However, there was no difference between paper point- and compressed air-dried cavities. 2. When cavities were dried with compressed air, dentin-bonded composite resin-filled cavities showed less apical leakage than IRM- or Super EBA-filled ones (p<0.05). 3. Regardless of the filling material, cavities contaminated with human blood showed significantly more apical leakage than those dried with compressed air after saline irrigation (p<0.05). 4. Outer half of the cavity showed larger dentin-filling interface gap than inner half did when cavities were filled with IRM or Super EBA. 5. In all the filling material groups, cavities contaminated with blood or dried with cotton pellets only showed larger defects at the base of the cavity than ones dried with paper points or compressed air.
Clay-coated rice seeds (clay-coated seeds A and B) were directly sown on dry paddy and their growth and yield were compared with the normal drill-sown seeds on dry flat paddy. In clay-coated seeds, germination was 1 day earlier and the emergence rate was higher up to 5% than that of normal drill-sown seeds. But the apparent number of seedling stand per $m^2$ was lower than that of normal drill-sown seeds, which is due to the smaller amount of seeding in clay-coated seeds. At the early growth stage, the plant height of clay-coated seeds A was taller than that of drill-sown seeds, while the plant height of clay-coated seeds B was 0.7 cm shorter than that of drill-sown seeds. At the late growth stage, however, the difference was insignificant in both cases. The maximum tillering stage was 10 days earlier in drill-sown seeds. Lodging index was the lowest in clay-coated seeds B and there was no difference between clay-coated seeds A and drill-sown seeds. The ratio of stem base weight, culm diameter and culm wall thickness were higher in clay-coated seeds, while the lower internodes (4th, 5th and 6th) length was shorter in claycoated seeds than in drill-sown seeds. In clay-coated seeds, the number of panicle per $m^2$ was smaller, while the number of spikelet per panicle was a little larger than in drill-sown seeds. The rate of ripened grain and brown rice 1,000 grain weight were lower in the clay-coated seeds, thus the yield was $98{\sim}99%$ level of drill-sown seeds. Considering that the amount of seeding in clay-coated seeds was two-thirds of that in drill-sown seeds, it is expected that clay coating method could become an additional technique for direct seeding cultivation.
Park, Jae-Hyeon;Yoon, Young-Han;Lee, Jae-Man;Song, Hee-Yeon;Ju, Jin-Hee
Journal of Environmental Science International
/
v.28
no.6
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pp.545-552
/
2019
Tropaeolum majus, with a high decorative and food demand for vertical greening systems, has been utilized to revitalize urban agriculture. The effects of number of non-woven fabrics in a non-water environment and the adaptability of T. majus to this system were investigated. Planting ground composition of the container-type wall vertical greening system was made using non-woven fabric in one, two, three, or four layers. The results showed that the soil water content remained the highest when the non-woven fabric comprised 4 sheets. The morphological properties showed more growth with the 4 sheets than with 1, 2, and 3 sheets. In terms of physiological characteristics, chlorophyll content was mostly high in the 4 sheets, while shoot fresh weight value was in the order of 3 > 4 > 2 > 1 sheet, and root fresh weight value was in the order of 4 > 2 > 1 > 3 sheets. The dry weight of the measured values in the shoot was in the order of 4 > 3 > 2 > 1 sheet while no clear difference was found in the root of each treatment. The difference in the flowring characteristics was not different, but in evaluating the characteristics as a whole, the growth in the three layers of non-waven fabric was the best. In addition, the soil moisture contents and the growth characteristics were statistically significant as a positive correlation between the groups. Thus, greater the non-woven fabric, the higher is the adaptability of T. majus to dry stress under soil water-free conditions by maintaining soil moisture content. This showed that it represented an effective alternative as a method of vertical greening system for lower maintenance urban agriculture.
In order to optimize a method to determine the firmness of Chinese cabbage, hardness of midrib tissues was examined based on their chronological order of emergence. Texture measurement using volodkevich bite jaws gave a consistent and highest regression ($r^2=0.85$) between firmness and the order of leaf emergence, while blade set, cylinder probe, and crisp fracture support rig showed a lower coefficient of determination. Thickness of midrib tissue within an individual head from 16 cultivars of Chinese cabbage was positively correlated with the order of emergence, becoming thinner toward inner leaves. Mean thickness of midrib tissue from the head ranged from 7.74 mm for 'CR-shingshing' and 9.28 mm for 'Norangyeorum'. The covariance of leaf thickness within a head was highly cultivar-dependent, ranging from 23.6% for 'Chihili' and 5.8% for 'Bulam'. Firmness of the midrib tissue, defined as maximum peak height per tissue thickness, became higher from outer to inner leaves, showing $2^{nd}$ order of regression. Mean firmness of the midrib tissue from individual head varied from 1.58 N for 'Rangno' to 3.46 N for 'CR-shingshing'. The $10^{th}$ or $11^{th}$ leaf brought the best correlation coefficient (r = 0.81) between firmness of an individual leaf and the mean firmness of the entire leaves in a head, suggesting a reliable and rapid method to estimate the firmness of a head in lieu of examining all leaves in the head. The relationship between firmness of midrib tissue and dry mass ($r=0.70^{**}$) as well as cell wall content ($r=0.58^*$) of the head were positively correlated. Results obtained from the present study suggested that a new method to determine midrib firmness would enable to clarify the relationship between textural quality of fresh Chinese cabbage and their processed product, 'Kimchi'. It will also be important to apply this method to screen textural quality of various genotypes under breeding programs.
Assoumaya, C.;Boval, M.;Weisbecker, J.L.;Saminadin, G.;Archimede, H.
Asian-Australasian Journal of Animal Sciences
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v.20
no.6
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pp.914-919
/
2007
The effect of the addition of exogenous fibrolytic enzymes (mainly xylanase and cellulase activities, 15 ml/15 kg of fresh forage), on intake, total tract digestibility and nylon bag degradability of a chopped fresh Digitaria decumbens grass was studied at 2 stages of regrowth ( 21 and 56-day old grasses). Moreover, comparisons between ground and chopped grass were done using the nylon bag degradability method. DM intake (g/kg $BW^{0.75}$) and organic matter total tract digestibility for control and enzyme treatments respectively were 69.1 vs. 65.9 (p>0.05) and 0.723 vs. 0.727 (p>0.05) with the 21-day old regrowth. Based on the same parameters, values for the 56-day old grass were 58.1 vs. 52.7 (p>0.05) and 0.621 vs. 0.591 (p>0.05). Nylon bag degradation at 24 h of the dry matter for control versus enzyme treatments were 0.653 vs. 0.70 (p<0.05) and 0.644 vs. 0.733 (p<0.0001) for the 21-day old chopped and ground forage respectively, whereas with the 56-day old grass, corresponding values were 0.321 vs. 0.392 (p<0.0001) and 0.463 vs. 0.481 (p>0.05). The positive impact of exogenous fibrolytic enzymes (EFE) on degradability of the young and ground pangola grass may suggest that in some cases, enzyme accessibility to potentially digestible cell wall is a limiting factor in their digestion.
Musco, Nadia;Koura, Ivan B.;Tudisco, Raffaella;Awadjihe, Ghislain;Adjolohoun, Sebastien;Cutrignelli, Monica I.;Mollica, Maria Pina;Houinato, Marcel;Infascelli, Federico;Calabro, Serena
Asian-Australasian Journal of Animal Sciences
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v.29
no.1
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pp.51-61
/
2016
In order to provide recommendations on the most useful forage species to smallholder farmers, eleven grass and eleven legume forages grown in Abomey-Calavi in Republic of Benin were investigated for nutritive value (i.e. chemical composition and energy content) and fermentation characteristics (i.e. gas and volatile fatty acid production, organic matter degradability). The in vitro gas production technique was used, incubating the forages for 120 h under anaerobic condition with buffalo rumen fluid. Compared to legume, tropical grass forages showed lower energy (8.07 vs 10.57 MJ/kg dry matter [DM]) and crude protein level (16.10% vs 19.91% DM) and higher cell wall content (neutral detergent fiber: 63.8% vs 40.45% DM), respectively. In grass forages, the chemical composition showed a quite high crude protein content; the in vitro degradability was slightly lower than the range of tropical pasture. The woody legumes were richer in protein and energy and lower in structural carbohydrates than herbaceous plants, however, their in vitro results are influenced by the presence of complex compounds (i.e. tannins). Significant correlations were found between chemical composition and in vitro fermentation characteristics. The in vitro gas production method appears to be a suitable technique for the evaluation of the nutritive value of forages in developing countries.
The value of Jeju Batdam has been reexamined as it is listed as a nationally important agricultural heritage and globally important agricultural heritage systems. However, it is already exposed to threats such as reduction of agricultural population and cultivation area. Despite efforts like the agricultural heritage system to preserve traditional agriculture, there is few basic investigation into the current status of Jeju Batdam. The purpose of this study is to estimate the length of Jeju Batdam. We used the continuous cadastral map of Jeju area to extract the boundaries of the field lot. In the cadastral map, the farmland was selected by selecting dry fields, paddy fields and orchards. 300 sample site were selected from the extracted farmland, and the boundaries between the Internet map and the parcels were superimposed and the differences were confirmed on the drawing. After that, field survey was conducted to confirm the boundary of the parcels and the existence of actual Batdam. It is estimated that the length estimated from this study is at least 23,983km and maximum 142,353km, which is at least 1,830km longer than 22,108km announced in 2007. Since Jeju Batdam is based on land parcel boundary, it is an objective and efficient method to utilize intellectual information. In addition, because it is subordinate to farmland, new information can be extracted reflecting the change of land use and make the spatial database based on the cadastral maps.
Kim, Do-Hoon;Cha, Min-Hyuck;Lee, Dea-Su;Kim, Kyung-Ryeol;Lee, In-Mo
Journal of Korean Tunnelling and Underground Space Association
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v.12
no.2
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pp.129-144
/
2010
The earth pressure acting on the vertical shaft is less than that acting on the retaining wall due to three dimensional arching effect. Thus, it might be essential to estimate the earth pressure actually acting on the shaft when designing the vertical shaft. In this paper, large-sized model tests were conducted as Part II of companion papers to verify the newly suggested earth pressure equation proposed by Kim et al. (2009: Part I of companion papers) that can be used when designing the vertical shaft in cohesionless soils as well as in c-$\phi$ soils and multi-layered soils. The newly developed model test apparatus was designed to be able to simulate staged shaft excavation. Model tests were performed by varying the radius of vertical shaft in dry soil. Moreover, tests on c-$\phi$ soils and on multi-layered soils were also performed; in order to induce apparent cohesion to the cohesionless soil, we add some water to the dry soil to make the soil partially-saturated before depositing by raining method. Experimental results showed a load transfer from excavated ground to non-excavated zone below dredging level due to arching effect when simulating staged excavation. It was also found that measured earth pressure was far smaller than estimated if excavation is done at once; the final earth pressure measured after performing staged excavation was larger and matched with that estimated from the newly proposed equation. Measured results in c-$\phi$ soils and in multi-layered soils showed reduction in earth pressures due to apparent cohesion effect and showed good matches with analytical results.
Microcapsules consisting of natural, biodegradable polymers for controlled and/or sustained core release applications are needed. Physicochemical properties of whey proteins suggest that they may be suitable wall materials in developing such microcapsules. The objectives of the research were to develop water-insoluble, whey protein-based microcapsules containing a model water-soluble drug using a chemical cross-linking agent, glutaraldehyde, and to investigate core release from these capsules at simulated physiological conditions. A model water soluble drug, theophylline, was suspended in whey protein isolate (WPI) solution. The suspension was dispersed in a mixture of dichloromethane and hexane containing 1% biomedical polyurethane. Protein matrices were cross-linked with 7.5-30 ml of glutaraldehyde-saturated toluene (GAST) for 1-3 hr. Microcapsules were harvested, washed, dried and analyzed for core retention, microstructure, and core release in enzyme-free simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) at 37$^{\circ}C$, A method consisting of double emulsification and heat gelation was also developed to prepare water-insoluble, whey protein-based microcapsules containing anhydrous milkfat (AMF) as a model apolar core. AMF was emulsified into WPI solution (15-30%, pH 4.5-7.2) at a proportion of 25-50% (w/w, on dry basis). The oil-in-water emulsion was then added and dispersed into corn oil (50 $^{\circ}C$)to form an O/W/O double emulsion and then heated at 85$^{\circ}C$ for 20 min for gelation of whey protein wall matrix. Effects of emulsion composition and pH on core retention, microstructure, and water-solubility of microcapsules were determined. Overall results suggest that whey proteins can be used in developing microcapsules for controlled and sustained core release applications.
Dong-Wook Lim;Ga-Yang Lee;Min-Jeong Jung;Byoung-Mok Kim;Joon-Young Jun
Food Science and Preservation
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v.30
no.6
/
pp.1043-1055
/
2023
This study was conducted to suggest an extraction method for preparing the extract from green tea leaves that possess enhanced antioxidant and antibacterial activities. Different ethanol concentrations were tested to recover phenolics and flavonoids, and 50% ethanol was the best under heat treatment (121℃, 15 min). The ethanol extract exhibited excellent DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and growth inhibition against B. cereus, B. licheniformis, S. aureus subsp. aureus, and A. hydrophila subsp. hydrophila. To enhance the antioxidant and antibacterial activities, cell-wall degrading enzymes (2.5% cellulose+2.5% pectinase, v/w dry sample) treatment and Saccharomyces cerevisiae fermentation were applied singly or in combination. The enzymatic treatment of green tea leaves notably increased extraction yield. However, the antioxidant and antibacterial activities of the extract were lower than those of the control (heat-treated 50% ethanol extract). In contrast, the yeast fermentation alone did not affect the yield, but enhanced antioxidant and antibacterial activities, contributing to the increase in the extract's total phenolic and flavonoid contents.
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